In the era of knowledge economy, the core status of knowledge elements is increasingly verified by practice in the process of NIS construction and innovation ecosystem optimization. This paper analyzes the game process of knowledge sharing and multiple dimensions including relational coupling intensity, relational quality and relational stability in innovation network. This paper focuses on analysis of the coordinating role between relational embeddedness and knowledge sharing, and explores legal, management and social coordination mechanism of knowledge sharing to improve the efficiency of the transfer of knowledge resource and innovation development among enterprises. The final goal is to provide policy advice for NIS construction and innovation ecosystem optimization.