Evaluation of scientific and technological innovation in colleges and universities is an important means to develop and improve the scientific and technological innovation ability . Based on the dynamic development of scientific and technological innovation in colleges and universities, this paper used the subjective and objective method of entropy weight-DEMATEL to evaluate the scientific and technological innovation ability of colleges and universities. The results showed that innovation potential was the main factor driving the development of scientific and technological innovation ability of universities in Jiangsu. Innovation vitality was the key link to restrict the development of innovation strength of university in Jiangsu.The unbalanced development among the innovation potential, innovation vitality and innovation strength were the main reason leading to the low ability of scientific and technological innovation of colleges and universities in Jiangsu.