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1.
通过实验确定活性焦化学需氧量(COD)吸附值测定方法中活性焦的称样量,据此建立针对活性焦性能指标的标准测定方法。实验中以邻苯二甲酸氢钾作为活性焦COD吸附值测定的标准物质,参照活性炭苯酚吸附值测定实验方法,进行活性焦COD吸附值测定的实验研究。对实验结果应用数据检验和回归分析,剔除异常值后,经t、F检验,得到活性焦COD吸附值与称样量之间的关联关系式。考虑满足COD吸附值测定数据受称样量影响变异系数小于5%,确定称样量为0.800~1.200 g,从而得到选定1.000 g作为测定方法中活性焦的合理称样量。  相似文献   

2.
唐睿谦 《广西教育》2015,(6):117-118
酶是具有高效催化能力的生物大分子物质,底物浓度相同时,酶的催化活性主要受温度、p H值等因素影响。温度一定时,酶活力最高时的p H值为该酶在该温度的最适p H。在不同p H值下,通过测定淀粉酶水解底物的程度,测定唾液淀粉酶的最适p H值。在不同p H条件下,淀粉被唾液淀粉酶水解,与碘液反应呈现颜色反应,通过对颜色的观察和分光光度计测量产物吸光值的大小,可知底物的反应程度,从而可以判断出唾液淀粉酶的最适p H。  相似文献   

3.
黄芪水提物对α-葡萄糖苷酶活性的抑制及其降血糖作用   总被引:1,自引:0,他引:1  
应用黄芪水提物与不同浓度的α-葡萄糖苷酶,不同浓度的底物PNP-glucoside进行反应,研究中药黄芪中α-葡萄糖苷酶抑制剂的抑制活性及其对正常小鼠血糖和糖耐量的影响.通过绘制酶量-反应速度曲线和双倒数曲线,确定其抑制剂类型,并对正常小鼠以40~160 mg.kg-1剂量灌胃后,观察其对血糖值和糖耐量的影响.结果表明,黄芪中α-葡萄糖苷酶抑制剂为可逆、非竞争性抑制;可降低正常小鼠血糖值,并且以80 mg.kg-1的剂量时血糖值最低,还可显著提高小鼠糖耐量.黄芪水提物中含有α-葡萄糖苷酶抑制剂成分,可用于糖尿病治疗.  相似文献   

4.
目的:观察通心络胶囊对肾病综合征患者凝血酶活性、纤维蛋白原的影响.方法:配对及肝素治疗对照研究.受试者均接受血常规、尿常规、24h尿蛋白定量、血浆肝功能、血脂、肾功能、凝血酶活性、纤维蛋白原浓度测定.结果:口服通心络胶囊可改善高凝状态,抑制凝血酶活性,降低纤维蛋白原浓度.结论:对于肾病综合征患者在有效控制蛋白尿的同时,加服通心络胶囊可有效抗凝,预防血栓、栓塞并发症出现.  相似文献   

5.
通过酶活性测定和紫外-可见光谱研究了胱氨酸对烟草多酚氧化酶(简称PPO)活性的影响.结果表明,胱氨酸对烟草多酚氧化酶有抑制作用,其机制是胱氨酸中的硫醚与烟草多酚氧化酶活性中心的铜离子配位,但底物或产物抑制胱氨酸与多酚氧化酶的结合;胱氨酸还可以与酶促反应产物结合生成无色复合物.胱氨酸抑制烟草PPO的过程可分为两步:首先胱氨酸与烟草PPO结合生成中间产物,中间产物仍具有一定的酶活性;然后中间产物结构进一步发生变化,成为无活性的形式.  相似文献   

6.
铜对过氧化氢在硫酸介质中氧化甲基橙的褪色反应有催化作用。本文用吸光光度法进行铜(Ⅱ)的测定,取得满意效果,灵敏度达9.00×10~11g/ml,测定范围0~2.0μgCu2十/50ml。并用本法测定发中Cu(Ⅱ)的含量,报道2-4岁男童发中Cu(Ⅱ)的测定值。  相似文献   

7.
几种抗氧化剂对菜籽油的抗氧化效果的研究   总被引:1,自引:0,他引:1  
采用烘箱贮藏法,以菜籽油为底物,测定了几种抗氧化剂的抗氧化活性,结果显示,植酸有很强的抗氧化活性,略优于丁基羟基茴香醚(BHA),但明显优于乙氧基喹琳(EQ)。  相似文献   

8.
本文介绍了一种速率法测定辣根过氧化物酶的方法。以对苯二胺和过氧化氢为底物经辣根过氧化物酶作用生成紫红色化合物.用440nm 单色光进行光电比色,每隔30秒钟记录一次吸光度值,直至3分钟为止。绘出吸光度时间关系曲线。求出斜率后,从标准工作曲线中查得样品中辣根过氧化物酶的含量。该方法可用于竦根过氧化物酶制备过程中每一操作步骤中酶的比活性测定。本法也可用于其他过氧化物酶的测定。  相似文献   

9.
为判定原料肉的新鲜度,建立一种定量测定原料肉中微量氨的方法。采用紫外分光光度法,以一氯胺为测定对象,探究原料肉氨含量与吸光度的关系。结果表明,在pH值为9的缓冲溶液中,1.0 mL肉浸液与1.0 mL次氯酸钠溶液室温下反应,于243 nm处测定吸光度,在0.00~10.00μg/25 mL时氨浓度与吸光度呈现良好的线性关系,据此建立了一种测定原料肉中微量氨的紫外分光光度法。实际应用表明,该方法不仅精确度较高,且操作简便、测定迅速、成本低廉,适宜在中小型肉类加工企业推广应用。  相似文献   

10.
在盐酸介质中,碘酸钾与钙红反应褪色程度与碘酸根浓度在一定范围内呈线性关系,以此建立了碘的光度测定新方法。方法的最大吸收波长在510nm处,碘定量测定的线性范围为0.3mVL,摩尔吸光系数ε=1.3×10^4L/mol·cm。本法所得结果和国家标准(GB5009.24.85)中规定食盐中碘含量的测定方法相当。  相似文献   

11.
A New Potent Inhibitor of Thrombin from the Leech Haemendipsa Yanyuanensis   总被引:1,自引:0,他引:1  
1 Introduction Thrombusdiseaseofheartandbrainbloodvesselhasbecomeakillerofman .Becauseofthesideeffectsofhaemorrhageortheshorthalf lifeinvivo ,thepre sentanticoagulantdrugsareunsatisfied ,andsearch ingfornewanticoagulantswithhigherefficientandmorestabileha…  相似文献   

12.
After screening 368 toddlers and selecting 77 into extremely inhibited, extremely uninhibited, and intermediate groups, 63 children (82%) were followed up at 4 and 7 years. Minority subgroups of both the inhibited and uninhibited children showed continuity on outcomes consisting of questionnaire measures of shyness, inhibitory control, and impulsivity, as well as multiepisode observational measures of behavioral inhibition and exuberance. Change from both inhibited and uninhibited status from the toddler age was more common than remaining extremely inhibited or uninhibited, but that change was largely constrained to the middle of the distribution.  相似文献   

13.
目的:以邻苯二酚为底物,研究白藜芦醇在不同温度与浓度下对酪氨酸酶的抑制作用.方法:用冰丙酮提取新鲜雪梨中的酪氨酸酶,-20℃以下保存.将95%白藜芦醇用60%乙醇配制成不同浓度的溶液,测定白藜芦醇浓度改变、温度变化对酪氨酸酶活性的影响.结果:酪氨酸酶活性在30℃时酶活性最好,且白藜芦醇对酪氨酸酶有较强的抑制作用,其半数抑制浓度(IC50)为5.3mmol/L.结论:白藜芦醇有较明显的酪氨酸酶抑制作用.  相似文献   

14.
多酚氧化酶是一种氧化还原酶,主要与植物色素的生成及其产品的色变有关,有利于水果或作物营养价值的保持.本论文以绿豆嫩叶多酚氧化酶粗提液为研究对象,采用不同条件下的磁感应强度的稳恒磁场处理,研究其对多酚氧化酶活性的影响.实验过程分别选用不同的磁感应强度以及时间处理,结果表明:在测酶活性过程中,酶与底物反映很快,1min时底物已全部反应,吸光度也不再增加.在反应初,对照组吸光度为0.8822.处理条件为1.6T15min时,磁场处理对PPO活性促进作用最明显,吸光度为1.0023.反应初始阶段完成以后,对照组酶活力为0.668U.处理条件为0.8T10min时,磁场处理对PPO活性促进作用最明显,酶活力为0.781U.  相似文献   

15.
通过离子交换、凝胶过滤、亲和层析及反向高效液相色谱操作,分析鉴定了棒纹牛蛭体内主要抗凝血物质成分为凝血酶抑制蛋白,其组成是三个相对分子质量分别为。7528,7446和7016的小分子量蛋白质,综合分析后认为属水蛭素类物质.  相似文献   

16.
高等教育自学考试是具有中国特色的教育制度,自创立至今,已积累了大量的经验,本文主要从农村自学考试制度演化变迁的角度,分析多年来这一新型教育形式的发展规律,并结合甘肃省的实践,探索自学考试制度变迁的原因及变迁方向,构建影响农村自学考试的指标体系,对指标体系的效度进行检验,其目的是找出农村自学考试制度变迁的路径。  相似文献   

17.
为进一步研究野桑蚕多酚氧化酶的作用机理奠定基础,利用分光光度计检测了金属离子和几种抑制剂对野桑蚕(Bombyx mandarina)多酚氧化酶活性的影响,结果表明:槲皮素和硫脲对该酶的抑制浓度(IC50)分别为0.086 mmol/L和0.092μmol/L,Cu2+对该酶有强烈的抑制作用,而Zn2+、Ca2+和Mg2+有激活作用.  相似文献   

18.
4-month-old infants were specifically selected for patterns of affective and motoric reactivity that were hypothesized to be associated with later inhibited and uninhibited behavior. Infants were classified as high on motor activity and negative affect, high on motor activity and positive affect, or low on motor activity and affect. Brain electrical activity was assessed in these infants at 9 months of age, and behavior toward novelty was observed at 14 months of age. Infants who were high on motor activity and negative affect exhibited greater right frontal EEG activation at 9 months of age and inhibited behavior at 14 months of age. Infants classified as high motor/high positive at 4 months of age exhibited uninhibited behavior at 14 months of age. No relations were found between frontal asymmetry at 9 months of age and inhibited behavior at 14 months of age. However, greater activation in both the left and right frontal hemispheres was associated with higher inhibition scores at 14 months of age. These findings are discussed in terms of the role that affective and physiological reactivity may play in the development of social behavior during toddlerhood.  相似文献   

19.
Two components of anticoagulant protein were isolated from the leech Haemendipsa yanyuanensis by heparin agarose affinity chromatography and ultracentrifugation,The determination of anticoagulant activity and characterization analysis of the protein using the method of chromogenic substrate indicates that the anticoagulant protein is thrombin-specific but not factor Xa-specific.The resylts lay a foundation for the research of the anticoaguklant mechanism and application of anticoagulant protein from H.yanguanensis.  相似文献   

20.
Inhibited and uninhibited types of children   总被引:10,自引:0,他引:10  
An initial group of 100 children who were not selected a priori on any behavioral features were observed in laboratory settings at 14, 20, 32, and 48 months and their behaviors coded for inhibition and lack of inhibition to the unfamiliar. The children who had been extremely inhibited or uninhibited at both 14 and 20 months differed significantly at 4 years of age in behavior and cardiac acceleration to cognitive stress. However, for the entire sample, there was no significant relation between degree of inhibited behavior at 14 or 20 months, on the one hand, and inhibition at 4 years of age, on the other, nor any relation between behavior and heart rate acceleration. These results suggest that the constructs inhibited and uninhibited to the unfamiliar refer to children who fall at the extremes of a phenotypic continuum from shyness and restraint to sociability and affective spontaneity.  相似文献   

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