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为研究祁术的组织培养及快速繁殖技术,选用祁术的叶片和下胚轴为外植体材料,接种于附加植物生长调节剂NAA,6-BA及其组合的MS固体培养基上.结果表明:下胚轴为外植体明显好于叶片,对于愈伤组织形成,叶片采用MS NAA(0.5mg/L) 6-BA(2mg/L);下胚轴MS NAA(0.5mg/L) 6-BA(2mg/L);MS NAA(0.5mg/L) 6-BA(1mg/L)为宜.对于叶片丛生芽愈伤组织培养基用MS NAA(0.2mg/L) 6-BA(2.8mg/L),下胚轴丛生芽愈伤组织培养基用MS NAA(0.01mg/L) 6-BA(0.1mg/L);MS NAA(0.05mg/L) 6-BA(0.1mg/L)为宜,诱导生根以1/2MS NAA0.3mg/L最佳,生根率达95%以上. 相似文献
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樱桃番茄的组织培养与快速繁殖研究 总被引：1，自引：0，他引：1
以樱桃番茄新品种“圣女”和“金珠”的叶片、茎段和幼芽为外植体进行试管培养 ,结果表明 :叶片、茎段和幼芽均可诱导形成愈伤组织 ,愈伤组织可分化出芽丛 ,腋芽可直接萌生 ,不定芽可诱导生根成完整植株 .经试验筛选出各培养阶段的最适培养基为 :(1)愈伤组织诱导 :叶片以MS Kt4mgL IAA8mgL为最佳 ;茎段和幼芽以MS NAA0 1mgL 6 -BA1 5mgL为最佳 ;(2 )愈伤组织芽的分化 :MS IAA0 2mgL 6 -BA2mgL ;(3)腋芽萌生 :MS 6 -BA2 5mgL IBA0 1mgL ;(4)生根 :无激素的Ms或Mso . 相似文献
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牡丹鳞芽离体培养的初步研究 总被引：4，自引：0，他引：4
以“银粉金鳞”鳞芽为材料进行离体培养,研究了植物生长调节剂6-BA和NAA对鳞芽不定芽诱导分化、增殖培养的影响．结果表明：MS＋Ca^2＋ ＋6-BA1．0mg／L＋NAA0．3mg／L为丛生芽诱导最佳培养基,诱导率高达81．67％;MS＋Ca^2＋ ＋6-BA1．6mg／L＋NAA0．2mg／L为最佳增殖培养基．增殖系数为3．21． 相似文献
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柏林百合组织培养的研究 总被引：1，自引：0，他引：1
朱建华 《宁波职业技术学院学报》2003,2(1):76-78
对柏林百合品种组织培养试验结果表明：初代培养的最佳培养基为MS 6-BA1．0mg／l(单位下同) NAA0．5；继代培养的配方为MS 6-BA2．0 NAA0．01；生根培养的配方为MS IAA0．5 活性炭5g／I；驯化的最佳基质组合为珍珠岩：腐殖土(1：1)。 相似文献
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以黄荆种子作为实验材料,分别研究不同质量浓度的植物激素和植物生长调节剂对黄荆种子萌发及根茎叶等生理指标的测定。通过研究黄荆种子在不同处理下萌发的最佳条件,以筛选适合黄荆种子萌发的植物激素和植物生长调节剂,为黄荆的繁殖培育提供依据。结果表明：在棉花、土壤、纱布3种不同的基质处理中,黄荆种子均能萌发,但在土壤基质中的发芽效果最好;在不同质量浓度的植物激素和植物生长调节剂处理中,NAA、IAA、GA3、6-BA处理都有利于黄荆种子萌发,其中,200 mg/L的GA3处理对黄荆种子的发芽和生长效果最好;在不同的植物激素和植物生长调节剂浸种时间中,用GA3浸泡处理6 h可以对黄荆种子的发芽率、发芽指数、活力指数以及对黄荆幼苗的平均根长有促进作用,效果最好。 相似文献
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菊花花瓣的组培快繁技术研究 总被引：2，自引：0，他引：2
以菊花的花瓣作外植体进行组培快繁技术研究,结果表明:外植体用0.1%升汞消毒10min为宜;诱导愈伤组织的最佳培养基为MS 6-BA 1.5mg/L NAA0.1mg/L;丛生芽形成的最佳培养基为MS 6-BA1.5mg/L NAA0.1mg/L;用1/2MS NAA0.5mg/L作生根培养基,生根率可达100%. 相似文献
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二色补血草的组织培养与快速繁殖 总被引：6，自引：0，他引：6
以二色补血草的茎为试材,采用MS和LS两种基本培养基,组配不同浓度的植物生长激素,诱导植株再生。本文探讨了培养基和激素对诱导愈伤组织和不定芽的影响,找出了较适宜茎诱导植株再生的基本培养基为MS。最佳生长激素6-BA浓度为0.3～0.6mg/L。初步建立了二色补血草的茎诱导一次成芽培养基为MS+6-BA0.3～0.6mg/L+NAA0.05mg/L+Augar30g/L;对诱导再生植株生根的几种激素也进行了研究。结果显示:最佳生根激素为IAA,其适宜浓度为0.2mg/L。生根培养基为1/2MS〕IAA0.2mg/L+Sugar20g/L。 相似文献
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以密叶罗勒的茎尖为外植体,以期筛选出诱导愈伤组织分化和丛生芽生根的最佳培养基。同时研究了密叶罗勒组织培养中材料的消毒时间对消毒效果的影响。试验结果表明,培养基MS＋6-BA5．0mg/L＋NAA0．1mg/L是愈伤组织诱导分化相对较好的培养基;培养基MS＋6-BA0．3mg/L＋NAA0．6mg／L是罗勒生根培养较好的培养基。在以75％的酒精消毒30秒后,用0．1％的升汞消毒2min,对试验材料的消毒效果相对较好。 相似文献
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以牛大力茎段离体培养的无菌芽作为试验材料,以MS为基本培养基,进行双因子和三因子激素组合处理比较试验,探讨外源激素因子对牛大力离体培养芽增殖的影响。结果表明:在双因子激素组合处理中,与NAA0.2mg/L组合的6-BA适宜浓度为3.0mg/L,芽增殖倍数分别为3.70倍(30d)、5.43倍(60d);KT适宜浓度为4.0mg/L,芽增殖倍数分别为2.03倍(30d)、2.54倍(60d);与6-BA3.0mg/L组合的生长素IAA适宜浓度为0.2mg/L,芽增殖倍数分别为4.38倍(30d)、6.55倍(60d)。在三因子激素组合处理中,对牛大力芽增殖效应依次为:6-BA+IAA+TDZ组合处理>6-BA+IAA+CPPU组合处理>6-BA+IAA组合处理(作对照);适宜的三因子激素组合为6-BA3.0mg/L+IAA0.2mg/L+TDZ0.03mg/L,其芽增殖倍数分别高达6倍(30d)和9.65倍(60d)。 相似文献
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罗艳萍 《读与写:教育教学刊》2008,5(6)
The communication of people partially is the communication of cultures. Culture has a direct effect on international commercial activities in all aspects. Different conceptions about time, space, equality, law and the like, lead people to deal with things in different ways. So to know cultures of the counterpart is to facil-itate our enterprises so as to have a smooth and successful communication in commercial activity. 相似文献
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Richard Denner 《海外英语》2007,(4):14-15
Rosco and I wait for the fishermen to return.I sit at a wooden bench near the store at Mt.Baker Resort and watch the clouds change shape. Rosco has my belt around his neck and an eight foot tow chain hooked to a tree. Dogs must be on a leash. Ducks and rabbits are loose. 相似文献
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Given a graph G,a subgraph C is called a clique of G if C is a complete subgraph of G maximal under inclusion and |C|≥2. A clique-transversal set S of G is a set of vertices of G such that S meets all cliques of G. The clique-transversal number, denoted as TC (G), is the minimum cardinality of a clique-transversal set in G. The clique-graph of G, denoted as K (G), is the graph obtained by taking the cliques of G as vertices, and two vertices are adjacent if and only if the corresponding cliques in G have nonempty intersection. Let F be a class of graphs G such that F={G|K(G) is a tree}. In this paper the graphs in F having independent clique-transversal sets are shown and thus TC (G)/|G|≤1/2 for all G ∈ F. 相似文献
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陈美曦 《山东教育学院学报》2008,23(2):103-107
Different nations have different social cultures, and the social culture of every nation is developing with the development of the nation, language is greatly influenced by social culture, which enables language to possess national and historical characteristics. Word is the smallest, independent, meaningful linguistic unit of language. It can well reflect the national and historical characteristics. From synchronic aspect, the social and cultural influence on word meaning is mainly embodied in conceptual and associative meanings of word. The incorrespondence of word meaning is a phenomenon that exists in both conceptual and associative meanings of word among different languages. From diachronic aspect, the development of social culture causes some changes of word meaning to some extent. Therefore, social culture plays a key role in vocabulary learning and mastery of a language. 相似文献
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邱崇丙 《唐山师范学院学报》2014,(1):21-23
汉字的六书,《说文》对“转注”一类,语焉不详。后世学者提及转注,也仅限于许慎所举出的例字。《汉字例说》一文,作者从转注的角度综合考虑,对部分现代常用字作了分析,跟传统的解释有所不同。希望能抛砖引玉,互相切磋,以推进学术研究。 相似文献
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《海外英语》2007,(4):36
There are numbers of crossroads on our long and unpredictable life journey where we totally have no idea about which direction to choose. No matter what our decision is, we should not turn back, but face the music and go ahead instead. I am this kind of girl who always does try without regretting, one example is how I dealt with my love. 相似文献
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Migration occurs behind a variety of reasons and has a great effect on the whole world. People may migrate in order to improve their economic situation, or in order to escape civil strife, persecution, and environmental disasters. The impact of migration is complex, bringing both benefits anddisadvantages. This paper briefly talks about the causes of migration, the allocation of benefits, and the ways in which individual countries and the international community deal with this important subject. 相似文献
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Anton Franks 《English in Education》1999,33(2):39-49
This paper looks at the way drama works to bring a model of an arts curriculum into reality. He also shows how drama is valuable when discussed as a separate, autonomous subject in the school curriculum as opposed to one subsumed within English. He argues for a view of the arts and gives an example of a sequence of work in an English GCSE class based around Arthur Miller's play View from the Bridge. From this he draws out several strands about the relationship of the drama curriculum to the individual students and teachers, the school, and to culture. He also emphasises the primacy of performance in drama education. 相似文献