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1.
Objective: to explore a new serological method for detectingHelicobacter pylori (H. pylori) infection. Methods: Serum soluble antigen ofH. pylori was detected by using avidin-biotin ELISA technique to evaluate the status ofH. pylori infection and for comparison with rapid urease test (RUT), histologic examination and serology. Results: The sensitivity,
specificity, positive predictive value and negative predictive value were 77.46%, 91.07%, 91.67% and 76.12%, respectively.
The prevalence rate of serumH. pylori soluble antigen in 138 patients undergoing endoscopy was similar to the rate obtained by14C-UBT methods (P>0.05). Conclusions: The detection of serumH. pylori soluble antigen(HpSAg) could be used as a new serological method which is accurate, and convenient, not affected by the memorizing
reaction of serum antibody; is more sensitive, more specific and suitable for clinical diagnosis, and evaluation of eradication
and for follow-up ofH. pylori as well as for detection in children and pregnant women.
Project supported by Zhejiang Provincial Health Bureau (No. 2000A118), China 相似文献
2.
Objective: To evaluate the interaction between serum levels of soluble intercellular adhesion molecule-1 (sICAM-1) and Helicobacter pylori (H. pylori) infection in patients with chronic gastritis and peptic ulcer. Methods: The serum levels of sICAM-1 in 205 patients with chronic gastric diseases were detected by ELISA method and the status of H. pylori was determined by histologic examination, RUT, 14C - UBT, and serology. The sera obtained from 18 healthy volunteers served as controls. Results: The serum levels of sICAM-1 were significantly higher in patients with H. pylori positive than those of H. pylori negative (889.43±32.52 ng/ml vs. 747.07±30.45 ng/ml, P<0.05). The serum levels of sICAM-1 in patients with mild, moderate and severe infection of H. pylori were 841.68±72.36 ng/ml, 905.43±37.59 ng/ml and 1012.54±49.34 ng/ml,respectively (P<0.05). The serum levels of sICAM-1 proved to be significantly correlated with the density of H. pylori colonization in gastric mucosa (rs =0.316, P<0.001). The serum levels of sICAM-1 in patients with chronic gastritis and peptic ulcer were significantly higher than those in healthy controls (P<0.05). Conclusions: These results indicated that H. pylori infection up-regulates the expression of sICAM-1. 相似文献
3.
Objective: To evaluate the interaction between serum levels of soluble intercellular adhesion molecule-1 (sICAM-1) andHelicobacter pylori (H. pylori) infection in patients with chronic gastritis and peptic ulcer. Methods: The serum levels of sICAM-1 in 205 patients with
chronic gastric diseases were detected by ELISA method and the status ofH. pylori was determined by histologic examination, RUT,14C-UBT, and serology. The sera obtained from 18 healthy volunteers served as controls. Results: The serum levels of sICAM-1
were significantly higher in patients withH. pylori positive than those of H. pylori negative (889.43±32.52 ng/ml vs. 747.07±30.45 ng/ml,P<0.05). The serum levels of sICAM-1 in patients with mild, moderate and severe infection ofH. pylori were 841.68±72.36 ng/ml, 905.43±37.59 ng/ml and 1012.54±49.34 ng/ml, respectively (P<0.05). The serum levels of sICAM-1, proved to be significantly correlated with the density ofH. pylori colonization in gastric mucosa (r
s=0.316,P<0.001). The serum levels of sICAM-1 in patients with chronic gastritis and peptic ulcer were significantly higher than those
in healthy controls (P<0.05). Conclusions: These results indicated thatH. pylori infection up-regulates the expression of sICAM-1. 相似文献
4.
INTRODUCTION Helicobacter pylori (H.pylori) was grouped as a class I carcinogen by the International Agency for Research on Cancer in 1994. A direct relation be-tween H.pylori infection and gastric carcinogenesis was demonstrated in 1998 in an experimental animal model (Watanabe et al., 1998). However, the role of H.pylori in human gastric carcinogenesis is sup-ported almost exclusively by epidemiological data and prospective histopathological studies. So far the mechanism of H.pylor… 相似文献
5.
Objective: To investigate the distribution of H. pylori antigens in the gastric mucosa in patients with H. pylori infection, and the relationship between the distribution and gastric cancer. Methods: Of 112 patients confirmed by pathological study to have chronic superficial gastritis, precancerous changes (chronic atrophic gastritis, intestinal metaplasia or atypical hyperplasia) and gastric cancer, 28 were H. pylori negative and 84 were H. pylori positive. H. pylori antigens in the gastric mucosa were detected by immunohistochemistry. Results: The H. pylori positive group, comprised 12 of 22 (50.0%) in the chronic superficial gastritis group, 22 of 25 (88.0%) in the precancerous changes group and 13 of 35 (37.1%) in the gastric cancer group. The positive rates of H. pylori antigens in the cytoplasm progressively increased, respectively at 0.0% (0/12),63.6% (14/22) and 84.6% (11/13) for the same groups (χ2=19.76, P=0.000); H.pylori antigens were located in the mucus layer and above the neck of the mucosal gland in 9 of 12 (75.0%) cases with chronic superficial gastritis, at the neck of the mucosal gland and the isthmus in 12 of 22 (54.5%) cases with precancerous changes, below the isthmus in 9 of 13 (69.2%) cases with gastric cancer (x2=25.30, P=0.000). In the H. pylori negative group, no H.pylori antigen was observed. Conclusion: With the progression of chronic superficial gastritis→precancerous changes→gastric cancer, H. pylori antigens progressively migrated from the outer part to the inner part of the cell, and from the superficial to the deep gastric mucosa. 相似文献
6.
通过对51名调查对象进行血清尿素酶抗体检测，同时进行胃镜检查，采取胃粘膜进行幽门螺杆菌培养，探索出一种更适于临床检测幽门螺杆菌感染的方法。 相似文献
7.
以鲁米诺还原纳米银作为信号分子标记癌胚抗原（CEA）,结合具有良好生物相容性的纳米金,固定癌胚抗原抗体于金电极上,成功建立了用于检测人血清中CEA含量的电化学发光（ECL）免疫分析方法.利用该方法可在浓度为0.1-50ng· mL-1的线性范围内稳定监测人血清中CEA的含量,检测限为0.05ng·mL-1.将该电化学发光传感器应用于健康志愿者的血清中进行CEA的检测,结果令人满意.相对标准偏差小于7.2％,平均回收率为97.2-102.9％,具有良好的稳定性和重现性. 相似文献
8.
An integrated approach utilizing proteomics and bioinformatics to detect ovarian cancer 总被引：6，自引：1，他引：6
INTRODUCTION Ovarian cancer is the most lethal gynecologicmalignancy. Poor survival rates are mainly attribut-able to late diagnosis. Most patients at diagnosis haveadvanced stage disease. The 5-year survival rate forlate clinical stage ovarian cancer is only 25%, but forearly stage disease, the survival rate can be as high as90%. CA125, the most widely used biomarker forovarian cancer, does not have a satisfying positivepredictive value. In early stage ovarian cancer,40%–50% pati… 相似文献
9.
目的：研究人体片形吸虫病ELISA检测试剂盒的检测效果。方法：采用云南大理本地采集的牛体片形吸虫成虫制备可溶性粗抗原,包被反应板,采用人体片形吸虫病ELISA法（FAWA-ELISA）进行研究,分别检测26份片形吸虫患者血清、102份其他寄生虫病患者血清和25份健康人血清,评价检测试剂盒的检测效果。结果：检测试剂盒的敏感性为100.0%、特异性分别为95.28%（95%CI：98.9%～91.2%）,约登指数分别为0.95。结论：FAWA-ELISA试剂盒检测效果较好,且成本相对较低,可适宜用于云南片形吸虫病流行区流行病学筛查,减轻病原学检测的工作量。 相似文献
10.
郭钢 《岳阳职业技术学院学报》2010,25(1):87-89
目的通过观察乙型肝炎病毒感染者腮腺组织中HBsAg、HBcAg和HBVDNA的表达情况,研究乙肝血清学指标与它们的相关性。方法免疫组化检测乙肝血清指标与腮腺组织中HbsAg、HbcAg和HBVDNA的相关性。结果23例腮腺组织中HBsAg阳性11例,阳性率为47．83％;HBcAg阳性10例,阳性率为43．48％。其中HBsAg和HBcAg同时阳性8例,占34．78％。14例免疫组化阳性患者检出HBVDNA9例,阳性率为64．29％,乙肝血清学指标与腮腺组织中HBsAg、HBcAg检测结果高度正相关（x^2=3．305,P〉0．05）,其Kendall等级相关系数rs=0．661（P〈0．05）。结论腮腺组织对HBV有较强的亲和力．唾液中HBV的出现可能源于受染的唾液腺组织,含HBV的唾液是乙型肝炎生活接触性传播的媒介。 相似文献
11.
Objective: The aims of this research were to purify and identify the 130 kDa (CagA) protein of H. pylori clinical isolate HP97002 and evaluate the relationships between the purified 130 kDa (CagA) protein and gastric diseases. Methods: The procedure for isolating the protein included 6 mol/L guanidine extract, size exclusion chromatography and elusion from gel. Sera of 68 patients with gastric diseases (44 with chronic gastritis,15 with atrophic gastritis,7 with peptic ulcer disease,2 with gastric cancer ) were obtained, and the serological response to CagA was studied by Western-blot using the purified protein. Results: The purified protein was 130 kDa and preserved good antigenicity and revealed basic isoelectric point about of 8.1. Among 68 sera, 43 sera could recognize the purified protein associated with chronic gastritis 47.7% (21/44),atrophic gastritis 86.7% (13/15),peptic ulcer disease 100% (7/7),gastric cancer 100% (2/2). Compared with each other, the difference was significant (χ2=13.327, P=0.004), and 130 kDa (CagA) protein was associated with severe gastric diseases (rs=0.442, P=0.001). Conclusion: The 130 kDa (CagA) protein was associated with severe gastric diseases. 相似文献
12.
目的:探讨男性尿道感染主要病原菌的种类及分布;评价10种抗生素对各检出菌的作用效果.方法:对临床确诊的男性尿道感染患者的分泌物标本按临床检验操作规程进行分离鉴定,同时对分离的细菌采用K-B法作药物敏感试验.抽取血清测沙眼衣原体IgM抗体.结果:1018例患者标本,934例检出病原菌,阳性率为91.75%.其中单菌感染82.76%;两种菌混合感染16.49%;三种菌共同感染0.75%.病原体为淋球菌、非淋球菌细菌、解脲支原体和沙眼衣原体共四类,分别为5.72%、73.77%、14.25%和6.26%.阳性球菌的感染(60.62%)在尿道炎中仍占重要地位,表皮葡萄球菌检出率最高,占所有鉴定病原菌的27.86%.药物敏感试验结果显示,临床常见9种843株细菌对10种抗生素的敏感率低于30%的菌株占55.56%(50/90),表现出极强的耐药性.结论:本地区男性尿道炎主要为非淋菌性尿道炎,细菌是导致男性尿道感染的主要病原菌,沙眼衣原体和解脲支原体感染不可忽视.细菌对抗生素的耐药性日趋严重,不容乐观.尿道感染的患者应兼做多种病原体检测,并通过药敏试验进行合理、科学的治疗才是控制该病的有效途径. 相似文献
13.
Ge Song Jia-yu Wu Yan Xie Yong Liu Ya-juan Qian Xue-ping Zhou Jian-xiang Wu 《Journal of Zhejiang University. Science. B》2017,18(12):1075-1082
Potato virus S (PVS) often causes significant losses in potato production in potato-growing countries. In this study, the ordinary strain of PVS (PVSO) was purified from PVS-infected potato plants and used as the immunogen to produce hybridomas secreting monoclonal antibodies (MAbs). Five highly specific and sensitive murine MAbs (1A3, 16C10, 18A9, 20B12, and 22H4) against PVS were prepared using conventional hybridoma technology. Using these MAbs, tissue print-enzyme-linked immunosorbent assay (ELISA), dot-ELISA, and double-antibody sandwich (DAS)- ELISA were developed for sensitive and specific detection of PVS infection in potato plants. The results of sensitivity assays revealed that PVS could be reliably detected in PVS-infected leaf crude extracts diluted at 1:10 240 and 1:163 840 (w/v, g/ml) in phosphate buffer saline (PBS) by dot-ELISA and DAS-ELISA, respectively. Twenty-two samples collected from potato fields in Yunnan Province, China were tested for PVS infection using the serological assays we had developed, and 14 of them were found to be positive. This indicates that PVS is now prevalent in potato fields in Yunnan Province. 相似文献
14.
目的分析全身炎症反应综合征(systemic inflammatory response syndrome,SIRS)评分、序贯器官功能(sequential organ failure assessment,SOFA)评分及快速序贯器官功能(quick sequential organ failure assessment,qSOFA)评分在脓毒症早期筛查中的意义。方法:选取2016年5月30日-2018年5月30日收治于我院重症医学科患者232例感染或疑似感染患者为研究对象,对其病例资料进行回顾性分析。进行SIRS评分、SOFA评分及qSOFA评分,分析三种评分方法对脓毒症患者阳性检出率及28天病死率。结果:232例感染或疑似感染患者中,SIRS组检出141例,阳性检出率60.8%,28天病死率16.3%,SOFA组检出80例,阳性检出率34.5%,28天病死率22.5%,qSOFA组检出42例,阳性检出率18.1%,28天病死率38%。结论:SOFA评分和qSOFA评分能对感染性患者预后能做出较为准确的评估,qSOFA评分最优。 相似文献
15.
目的找出GB-青霉素菌渣脱水的方法,测定菌渣中的含糖量.方放选用不同的沉淀剂,离心脱水,比较脱水量.用紫外分光光度计,采用苯酚-浓硫酸法测定水溶性糖含量,检测波长490nm,狭缝0.02mm.结果选用NaCl和CaO作为沉淀剂,脱水效果较为理想,水溶性糖含量为0.5%.结论用合适的脱水剂可使GB-青霉素菌渣顺利脱水,脱水率达67.5%. 相似文献
16.
郭兴刚 《襄樊职业技术学院学报》2014,(6):27-29
目的研究前列腺特异性抗原（Prostatespecific antigen,PSA）检测用于前列腺癌筛查的临床价值。方法收集1044例住院人群PSA检测资料与前列腺癌检出率进行统计分析。结果 1044例中有16.57%（173例）血清中PSA有不同程度增高,最高值在300 ng/ml以上,且随年龄增大其增高程度及增高比例逐渐增加;前列腺癌检出率为0.957%,在50~79岁人群检出率为6.4%。结论前列腺特异性抗原检测用作前列腺癌的常规筛查临床价值不高,用于高危人群筛查有较高的临床价值。 相似文献
17.
半数效量分析模型在小鼠血清溶血素测定中的应用 总被引：2，自引：0，他引：2
目的 应用半数效量分析模型建立小鼠血清溶血素半数溶血值(HC50)新的计算公式。方法 小鼠血清溶血素分光光度测定法测定正常小鼠血清在不同稀释度的溶血反应率,用半数效量分析模型对测定值进行曲线拟合,建立计算小鼠血清半数溶血值(HC50)的新方法并与原计算方法作统计学比较。 结果1)小鼠血清对数稀释倍数与溶血概率单位符合半数效量分析模型,曲线拟合良好,直线方程为 y=11.4238-2.1911x,r=0.9978,相关系数显著性检验P<0.01,由方程建立的小鼠血清半数溶血值(HC50)概率单位法计算公式为HC50 = lg-1[lg(N)+(5-y)/b]。2)概率单位公式法可在溶血反应率为2.9～97.8%的较大范围内准确计算血清样品溶血素的半数溶血值(HC50),与理论值的误差率范围为2.81～8.30%;与原方法计算结果(误差率范围为4.65～77.11%)比较,除溶血反应率为52.5%的测定值点外,各溶血反应率测定值点的计算结果误差率均有显著性差异(P<0.05)。概率单位公式法计算结果与光密度比值法比较,具有更高的准确性。 结论 小鼠血清对数稀释倍数与溶血概率单位符合半数效量分析模型,由此建立的概率单位公式法可在样品血清较大溶血反应率范围内准确计算血清样品溶血素的半数溶血值。 相似文献
18.
目的:探讨两种家兔急性肾功能衰竭(ARF)模型的方法学差异.方法:皮下注射1%HgCl2(1.5ml/kg.bw,H组)、肌肉注射50%甘油(5.6ml/kg.bw、8ml/kg.bw、10ml/kg.bw三种剂量,依次为G1组、G2组、G3组)复制家兔ARF模型,测定24h和48h血清尿素氮(BUN)和肌酐(Cre)水平,观察比较各组模型复制的成功率、存活率及稳定性.结果:G1、G2两组不能成功复制ARF模型;G3组BUN、Cre较H组降低(P＜0.05).但G3组的存活率及稳定性均显著高于H组(P＜0.05).结论:两种模型复制方法各有优缺点,应根据不同研究目的选择相应的模型. 相似文献
19.
为对心脏疾病患者进行实时心率监测,提出一种心率检测方法。采用 STM32 单片机作为控制芯片,传感器采用 SON1205 心率传感器。首先将心率信号输出成对应的方波,将此方波输入到单片机处理,然后通过设计的心率检测算法得出心率,再经过滤波算法处理,得到准确的心率,最后通过 LabVIEW 软件进行仿真实验,得到心率波形。实验结果表明,与高精度测量仪器相比,该方法误差值最多不超过 3,准确率达到 96%以 上。基于 STM32 的心率检测方法性能稳定,数据可靠,可用于日常心率检测。 相似文献
20.
[目的]了解胃癌患者血清中抗核抗体谱的特点.[方法]以鸡卵核为抗原,应用免疫印迹法对18份胃癌术前患者以及22份正常对照组(年龄和性别分布均无差异)的血清进行分析.[结果]胃癌术前患者血清中特异识别5条抗原条带的抗核抗体的阳性率显著高于正常对照组,靶抗原的蛋白分子质量及胃癌术前组血清中特异识别这5条抗原条带的抗核抗体的阳性率分别为:69KD(44.4％,8/18),62KD(50％,9/18),58KD(33.9％,7/18),44KD(22.2％,4/18),37KD(38.9％,7/18).[结论] 胃癌患者体内存在抗核抗体,其相对的靶抗原有多种. 相似文献