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1.
乳酸脱氢酶(LDH)是糖无氧代谢的重要酶,有5种同工酶,在各组织中不均匀分布形成特定酶谱.酶谱含量改变可判断运动对组织的影响.本文通过血液、肌肉LDH同工酶比较,观察不同组织在剧烈运动疲劳时变化规律及其相互关系.  相似文献   

2.
目的 :探讨补充不同剂量的谷氨酰胺对运动员机体各血液生化指标和血浆谷氨酰胺水平的影响。方法 :12名运动员随机分成 4组 ,进行 2周大强度训练 ,训练期间分别补充不同剂量的谷氨酰胺 (0 .1g/kgbodywt/day ,0 .2 g/kgbodywt/day ,0 .3g/kgbodywt/day)和安慰剂。采集运动员训练周期开始前第 1天及最后 1天训练前后的血液 ,分别测定肌酸激酶 (CK)、乳酸脱氢酶 (LDH)、血尿素氮 (BUN)、血氨 (Ammonia)及血浆谷氨酰胺(Gln)的值。结果 :大强度训练会引起血液生化指标肌酸激酶、乳酸脱氢酶、血尿素氮、血氨的显著上升 (P <0 .0 5 ) ,补充谷氨酰胺并不改善上述指标的变化。大强度训练会导致血浆谷氨酰胺水平显著下降 (P <0 .0 5 ) ,补充中剂量 (0 .2 g/kgBW /day)谷氨酰胺能显著提高运动后血浆谷氨酰胺水平 (P <0 .0 5 )。  相似文献   

3.
以Wistar雄性大鼠为研究对象,探讨不同运动方式对同龄大鼠代谢终产物乳酸（lactic acid）和血清乳酸脱氢酶（lactate dehydrogenase,LDH）、肌酸激酶（creatine kinase,CK）的影响。结果：经过8周的跑台运动训练后,与对照组相比,无氧、有氧训练组大鼠血乳酸水平出现非常显著性差异（P〈0.01）,无氧组的LDH活性值显著升高（P〈0.01）,而有氧组没有显著性差异;无氧训练组组内第8周与静息值相比出现非常显著性差异（P〈0.01）,而有氧训练组组内LDH活性始终无明显变化。经过8周的训练,无氧组大鼠血清中肌酸激酶的活性有了很大提高（P〈0.01）,组间组内均具有显著性差异;有氧组肌酸激酶的活性也有了一定的提高,但较无氧组变化较小;对照组几乎无明显变化。结论：经过8周的跑台训练后,两种训练方式均有效提高了大鼠的血乳酸水平。不同方式的跑台运动训练后,大鼠血清乳酸脱氢酶的活性变化出现在无氧组中,显示无氧组的运动强度更能刺激大鼠血清LDH水平。两个训练组大鼠血清CK值,横向组间比较和纵向组内对比均出现显著性变化,表明大鼠血清CK值对运动负荷量比较敏感;无氧组横向组间比较和纵向组内对比,均比有氧组有更显著的变化,表明大鼠血清CK值能够反映运动负荷强度的变化。  相似文献   

4.
以参加第 9届全运会决赛的安徽省散打队 1 2名运动员为研究对象 ,应用血乳酸 ( BLA)、血红蛋白 ( HB)、红细胞压积 ( HCT)、丙氨酸氨基转移酶 ( ALT)、门冬氨酸氨基转移酶 ( AST)、碱性磷酸酶 ( ALP)、尿素氮 ( BUN)、血尿酸 ( UA)、血肌酐( CRE)、乳酸脱氢酶 ( LDH)、线粒体门冬氨酸氨基转移酸 ( ASTM)、肌酸激酶同功酶( CKMB)、α-羟丁酸脱氢酶 ( HBDH)、肌酸激酶 ( CK)、声光反应时等生理生化指标 ,对运动员赛前训练过程中的运动强度、运动量、机能状态、心功能、肝功能和肾功能等进行了监测、诊断和评定。结果提示 :以上生理生化指标对散打赛前的训练控制具有重要意义。  相似文献   

5.
G804.7 20010553模拟高原训练对小鼠骨骼肌 LDH 同工酶谱的影响=Onthe effects Of mimic altitude training on micemuscle LDH isozyme patterns[刊,中,A]/李世成,田野//武汉体育学院学报.-2000.-34(3).-74-77参8(XH)高原训练//模拟训练//骨骼肌//乳酸脱氢酶//同工酶//代谢//鼠//动物实验  相似文献   

6.
支链氨基酸对运动力竭大鼠心肌损伤的保护作用   总被引：5，自引：0，他引：5  
以大鼠力竭运动为模型,研究了支链氨基酸(BCAA)对血清和心肌中乳酸脱氢酶(LDH),谷草转氨酶(GOT),肌酸激酶(CK),α-羟丁酸脱氢酶(α-HBDH)等酶的活性以及心肌中Ca2 ,Mg2 含量的影响。结果发现:力竭运动可造成大鼠血清和心肌中LDH,GOT,CK,α-HBDH 等酶的活性显著升高,心肌中Ca2 含量也显著升高,Mg2 含量显著下降。补充BCAA 可使大鼠力竭运动后血清和心肌中LDH,GOT,CK,α-HBDH 等酶的活性显著降低,心肌中Ca2 含量显著下降。提示,BCAA 可抑制大鼠力竭运动后血清和心肌中LDH,GOT,CK,α-HBDH 等酶的活力显著升高和心肌中钙超载。说明BCAA 有减少力竭运动后心肌损伤和维持心肌正常功能的作用。  相似文献   

7.
在备战全国柔道锦标赛的冬季训练期间的一个典型大运动量训练日,选用心律(HR)、血尿素(BU)、血乳酸(BLA)、血肌酸激酶(CK)、心肌型血肌酸激酶同工酶(CK-MB)、血红蛋白(HB)、血肌酐(CREA)和血谷草转氨酶(GOT)为测试指标,观察训练中及次晨变化.结果表明:女子柔道训练对磷酸原与糖酵解代谢作用有良好反应:女子柔道运动员对研究当日的身体负荷具有较好的适应性.  相似文献   

8.
目的探讨"高住低练"过程中,运动员安静时血清酶的变化规律.方法20名运动员随机分为高住低练组和低住低练组,每组10名,分别测定运动员在此过程中安静状态下血清肌酸激酶(CK)、乳酸脱氢酶(LDH)和谷丙转氨酶(ALT)的活性.结果在高住低练的初期,运动员血清CK、LDH活性明显高于对照组,而高住低练4周后,血清酶活性则明显低于对照组,并可连续到高住低练后2周.结论提示运动员在2 500m高度进行4周高住低练,机体对缺氧负荷和运动负荷的适应后,能降低由于运动引起的血清酶活性的升高,对防止肌肉组织的损伤和增加细胞膜的稳定性有一定的作用.  相似文献   

9.
叶春  罗晶  许豪文  何执静 《体育科学》2005,25(9):53-55,82
实验旨在观察预热休克对一次性大负荷运动造成大鼠骨骼肌运动性损伤是否具有保护作用。实验采用大鼠热休克模型,用物理加热方法使大鼠体温升高到40.5℃~41.5℃,持续10~15min,在室温下恢复24h后进行一次性大负荷运动(速度为27m/min,坡度0°,时间为90min)。通过对比预热和室温组大鼠运动后即刻、24h、48h血清中的肌酸激酶(CK)、乳酸脱氢酶(LDH),来确定预热休克对大鼠运动所致骨骼肌损伤是否具有保护作用,以及是否能够加速骨骼肌运动性损伤的恢复。结果发现,预热组运动后即刻、24h,血清中激酶和乳酸脱氢酶活性均比室温组低(P<0.05);运动后48h,预热组较室温组血清中肌酸激酶和乳酸脱氢酶活性略低,没有显著性差异,两组肌酸激酶值比室温基础组略高,也没有显著性差异;预热运动后48h组乳酸脱氢酶与室温基础组相比略高,没有显著性差异;室温运动后48h组乳酸脱氢酶较室温基础组明显偏高,具有显著性差异(P<0.05)。提示,预热休克对于一次性大负荷运动所致的大鼠骨骼肌运动性损伤具有保护作用,并且能够促进骨骼肌运动性损伤的恢复。  相似文献   

10.
目的:研究肌酸补剂对小鼠运动能力的影响及其机制.方法:昆明种雄性小鼠60只(15～17g),分为对照组(control group,CG)和肌酸补充组(3.0g/kg体重,creatine supplemented group,SG),每组30只.2周后,分别于运动前、定量负荷运动(负5%体重,游5min)后和力竭运动(负18%体重)后取材.石蜡切片HE染色统计肌纤维横截面积;测定血乳酸和肌乳酸、肝糖原和肌糖原及腓肠肌和血清中的肌酸激酶和乳酸脱氢酶.结果:肌酸补充组小鼠游泳至力竭时间显著高于对照组(P<0.01);定量负荷运动后,肌酸补充组血乳酸和肌乳酸增加幅度显著低于对照组;运动后肌酸补充组血清CK和LDH含量均显著低于对照组(P<0.05);运动前肌酸补充组肝糖原含量显著高于对照组(P<0.01).结论:肌酸补剂能减少运动小鼠血液和肌肉中乳酸堆积,有助于保护肌细胞膜的完整性,肌酸补充还可增加糖原贮备,从而延长小鼠游泳至力竭时间.  相似文献   

11.
目的探讨相对运动强度和绝对运动强度对血清酶活性变化的影响。实验方法受试者为急性心肌梗塞康复病人(PMIP)和正常人(HS) ,实验前后测定了他们血清乳酸脱氢酶(L DH)、乳酸脱氢酶同工酶1(L DH- 1)、肌酸激酶(CK)、肌酸激酶亚单位MB(CK- MB)活性。结果两组受试者运动前酶的活性均无显著差异(P>0 .0 5 ) ;实验中,PMIP与HS的相对强度分别为最大心率的85 .1%、86 .0 % (P>0 .0 5 ) ,绝对强度分别为跑台等级的5 .3与6 .3(P<0 .0 5 ) ,PMIP与HS运动后即刻酶活性与运动前相比均有显著上升(P<0 .0 5 ) ,但两组间无显著差异(P>0 .0 5 ) ,2 4 h后HS组的酶活性基本恢复到运动前水平,但PMIP组仍高于运动前水平。结论运动后酶活性上升的幅度与运动所达到的相对强度有关;强度达85 % HRmax的运动能引起血清酶活性的升高,但此时L DH- 1/L DH和CK- MB/CK的比值与运动前相比无明显变化;如果PMIP的康复运动量等同于测试中的运动量,他们第2天似应停止活动以便能够得到充分的恢复。  相似文献   

12.
Indirect markers of muscle damage and collagen breakdown were recorded for up to 9 days after a bout of concentric, followed by a bout of eccentric, muscle actions. Nine untrained participants performed two bouts of 50 maximum effort repetitions on an isokinetic dynamometer (angular velocity 1.05 rad.s-1, range of motion 1.75 rad). An initial concentric bout of muscle actions was followed by an eccentric bout 21 days later, using the same knee extensors. Concentric actions induced no changes in maximum voluntary isometric contraction force (MVC),nor induced any changes in the serum enzyme activities of creatine kinase, a lactate dehydrogenase isoenzyme (LDH-1), or alkaline phosphatase. Similarly, concentric actions induced no change in markers of collagen breakdown,namely plasma hydroxyproline and serum type 1 collagen concentration.In contrast,eccentric actions induced a 23.5 ± 19.0% (mean ± s) decrease in MVC immediately post-exercise (P< 0.05), and increased the serum enzyme activities of creatine kinase and LDH-1 to 486 ± 792 and 90 ± 11 IU.l-1 respectively on day 3 post-exercise, and to 189 ± 159 and 96 ±13 IU.l-1 respectively on day 7 post-exercise (all P < 0.05). Eccentric actions induced no significant changes in plasma hydroxyproline, but increased collagen concentration on days 1 and 9 post-exercise (48.6% and 44.3% increases above pre-exercise on days 1 and 9 respectively; both P < 0.05). We conclude that eccentric but not concentric actions may result in temporary muscle damage, and that collagen breakdown may also be affected by eccentric actions. With caution, indices of collagen breakdown may be used to identify exercise-induced damage to connective tissue.  相似文献   

13.
The aim of this study was to compare the effect of low-load resistance exercise (LLRE) with continuous and intermittent blood flow restriction (BFR) on the creatine kinase (CK), lactate dehydrogenase (LDH), protein carbonyl (PC), thiobarbituric acid-reactive substance (TBARS) and uric acid (UA) levels in military men. The study included 10 recreationally trained men aged 19 ± 0.82 years who underwent the following experimental protocols in random order on separate days (72–96 h): 4 LLRE sessions at a 20% 1RM (one-repetition maximum [1RM]) with continuous BFR (LLRE + CBFR); 4 LLRE sessions at 20% 1RM with intermittent BFR (LLRE + IBFR) and 4 high-intensity resistance exercise (HIRE) sessions at 80% 1RM. The CK and LDH (markers of muscle damage) levels were measured before exercise (BE), 24 h post-exercise and 48 h post-exercise, and the PC, TBARS and UA (markers of oxidative stress) levels were measured BE and immediately after each exercise session. There was a significant increase in CK in the HIRE 24 post-exercise samples compared with the LLRE + CBFR and LLRE + IBFR (P = 0.035, P = 0.036, respectively), as well as between HIRE 48 post-exercise and LLRE + CBFR (P = 0.049). Additionally, there was a significant increase in CK in the LLRE + CBFR samples BE and immediately after each exercise (Δ = 21.9%) and in the HIRE samples BE and immediately after each exercise, BE and 24 post-exercise, and BE and 48 post-exercise (Δ values of 35%, 177.6%, and 177.6%, respectively). However, there were no significant changes in LDH, PC, TBARS, and UA between the protocols (P > 0.05). Therefore, a physical exercise session with continuous or intermittent BFR did not promote muscle damage; moreover, neither protocol seemed to affect the oxidative stress markers.  相似文献   

14.
Indirect markers of muscle damage and collagen breakdown were recorded for up to 9 days after a bout of concentric, followed by a bout of eccentric, muscle actions. Nine untrained participants performed two bouts of 50 maximum effort repetitions on an isokinetic dynamometer (angular velocity 1.05 rad x s(-1), range of motion 1.75 rad). An initial concentric bout of muscle actions was followed by an eccentric bout 21 days later, using the same knee extensors. Concentric actions induced no changes in maximum voluntary isometric contraction force (MVC), nor induced any changes in the serum enzyme activities of creatine kinase, a lactate dehydrogenase isoenzyme (LDH-1), or alkaline phosphatase. Similarly, concentric actions induced no change in markers of collagen breakdown, namely plasma hydroxyproline and serum type 1 collagen concentration. In contrast, eccentric actions induced a 23.5+/-19.0% (mean+/-s) decrease in MVC immediately post-exercise (P < 0.05), and increased the serum enzyme activities of creatine kinase and LDH-1 to 486+/-792 and 90+/-11 IU.l(-1) respectively on day 3 post-exercise, and to 189+/-159 and 96+/-13 IU x l(-1) respectively on day 7 post-exercise (all P< 0.05). Eccentric actions induced no significant changes in plasma hydroxyproline, but increased collagen concentration on days 1 and 9 post-exercise (48.6% and 44.3% increases above pre-exercise on days 1 and 9 respectively; both P < 0.05). We conclude that eccentric but not concentric actions may result in temporary muscle damage, and that collagen breakdown may also be affected by eccentric actions. With caution, indices of collagen breakdown may be used to identify exercise-induced damage to connective tissue.  相似文献   

15.
Strenuous physical exercise of the limb muscles commonly results in damage, especially when that exercise is intense, prolonged and includes eccentric contractions. Many factors contribute to exercise-induced muscle injury and the mechanism is likely to differ with the type of exercise. Competitive sports players are highly susceptible to this type of injury. AM3 is an orally administered immunomodulator that reduces the synthesis of proinflammatory cytokines and normalizes defective cellular immune fractions. The ability of AM3 to prevent chronic muscle injury following strenuous exercise characterized by eccentric muscle contraction was evaluated in a double-blind and randomized pilot study. Fourteen professional male volleyball players from the First Division of the Spanish Volleyball League volunteered to take part. The participants were randomized to receive either placebo (n=7) or AM3 (n=7). The physical characteristics (mean+/-s) of the placebo group were as follows: age 25.7+/-2.1 years, body mass 87.2+/-4.1 kg, height 1.89+/-0.07 m, maximal oxygen uptake 65.3+/-4.2 ml.kg(-1).min(-1). Those of the AM3 group were as follows: age 26.1+/-1.9 years, body mass 85.8+/-6.1 kg, height 1.91+/-0.07 m, maximal oxygen uptake 64.6+/-4.5 ml.kg(-1).min(-1). All participants were evaluated for biochemical indices of muscle damage, including concentrations of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, creatine kinase (CK) and its MB fraction (CK-MB), myoglobin, lactate dehydrogenase, urea, creatinine and gamma-glutamyltranspeptidase, both before and 30 days after treatment (over the peak of the competitive season). In the placebo group, competitive exercise (i.e. volleyball) was accompanied by significant increases in creatine kinase (494+/-51 to 560+/-53 IU.l(-1), P < 0.05) and myoglobin (76.8+/-2.9 to 83.9+/-3.1 microg.l(-1), P < 0.05); aspartate aminotransferase (30.8+/-3.0 to 31.1+/-2.9 IU.l(-1)) and lactate dehydrogenase (380+/-31 to 376+/-29 IU.l(-1)) were relatively unchanged after the 30 days maximum effort. AM3 not only inhibited these changes, it led to a decrease from baseline serum concentrations of creatine kinase (503+/-49 to 316+/-37 IU.l(-1), P < 0.05) and myoglobin (80.1+/-3.2 to 44.1+/-2.6 IU.l(-1), P < 0.05), as well as aspartate aminotransferase (31.1+/-3.3 to 26.1+/-2.7 IU.l(-1), P < 0.05) and lactate dehydrogenase (368+/-34 to 310+/-3 IU.l(-1), P < 0.05). The concentration of CK-MB was also significantly decreased from baseline with AM3 treatment (11.6+/-1.2 to 5.0+/-0.7 IU.l(-1), P < 0.05), but not with placebo (11.4+/-1.1 to 10.8+/-1.4 IU.l(-1)). In conclusion, the use of immunomodulators, such as AM3, by elite sportspersons during competition significantly reduces serum concentrations of proteins associated with muscle damage.  相似文献   

16.
目的:研究补充FDP对递增运动负荷训练至力竭的运动性疲劳大鼠各时相心肌SOD、MDA、LDH 和CK的影响及其变化规律.方法:实验以SD大鼠为研究对象,测定其运动训练5周、力竭后即刻、力竭后6h、力竭后24h心肌中LDH、CK、SOD活性和MDA水平,安静对照组则测定其安静状态下上述相同的实验指标.结果:①中小强度运动训练5周后,FDP组心肌各指标与对照组相比没有显著性意义;②力竭后即刻及恢复各时相FDP组心肌CK和LDH活性均高于对照组;③力竭即刻及恢复各时相,FDP组心肌SOD活性和SOD/MDA的比值均显著高于对照组,MDA含量显著性低于运动对照组.结论:FDP能提高运动力竭后即刻及恢复期各时相心肌LDH、CK、SOD活性和SOD/MDA的比值,降低MDA的生成;说明FDP能促进心肌组织糖酵解,改善心肌的能量供应,提高抗脂质过氧化作用,对心肌细胞具有良好的保护作用.  相似文献   

17.
目的:探讨五星草胶囊对耐力项目运动员运动能力的影响。方法:将8名男子中长跑运动员分为实验组(服用五星草胶囊4名)和对照组(服用安慰剂4名),在正常训练期间,实验组每人每日早晚各服用五星草胶囊5粒,共服用10天。服药前一天和停药后次日上午测试受试者体成分、血清肌酸激酶(CK)、血尿素(BU)、睾酮(T)、血红蛋白(Hb)、红细胞计数(RBC)指标以及递增负荷实验的心率(HR)和血乳酸(Bla)。结果:实验组在服药后有氧运动后即刻的Bla明显下降(P<0.05),Bla最高值和Bla增值降低,运动后即刻的HR降低,12minHR的恢复率增加,Hb值明显增加(P<0.05),对照组在实验后T明显下降(P<0.05),其余指标在实验前后没有明显变化(P>0.05)。结论:服用五星草胶囊对改善运动员的身体机能状况有一定的效果,对耐力项目运动员有氧能力有一定的促进作用。  相似文献   

18.
剧烈运动对几种血清酶活性和T 细胞的影响   总被引：10，自引：0，他引：10  
通过对大学生剧烈运动时血清酶活性和剧烈运动及中等强度运动时外周血中Ｔ细胞总数变化的观察,发现：剧烈运动后血清磷酸肌酸激酶、磷酸肌酸同工酶、乳酸脱氢酶、α－羟丁酸脱氢酶的活性均显著升高,３ｈ后恢复。外周血中Ｔ细胞总数百分率剧烈运动后２ｈ显著下降,１２ｈ后基本恢复。中等强度运动后则无明显变化。结果提示：剧烈运动可导致骨骼肌和心肌细胞的损伤及细胞免疫功能下降。由此说明健身运动的最大心率不宜超过１８０～１９５次／ｍｉｎ。  相似文献   

19.
过度训练对大鼠血清心肌酶活性的影响   总被引：6，自引：0，他引：6  
为了探讨过度训练对机体心脏功能的影响及其机制 ,通过对大鼠进行力竭性运动训练建立大鼠的过度训练模型 ,测定血清中CK、CK -MB、HBDH的活性以及心肌组织中SOD活性和MDA含量。研究结果 :经过 8周的运动训练后 ,1h训练组中血清CK、CK -MB和HBDH活性与对照组相比有上升趋势 ,但无显著性差异 ;而过度训练组血清CK、CK -MB和HBDH活性均显著高于对照组和 1h训练组。与此同时 ,1h训练组心肌组织中SOD活性、MDA含量和SOD/MDA与对照组相比均无显著性差异 ;而过度训练组心肌组织中 ,MDA含量显著高于对照组 ,SOD活性和SOD/MDA均显著低于对照组和 1h训练组。结果表明过度训练对心肌细胞具有一定的损伤作用 ,过度训练使心肌组织中氧自由基的生成显著增加 ,这可能也是血清心肌酶活性升高的机制。训练过程中定期检测血清心肌酶的活性 ,对于了解运动员的机能状态 ,预防过度训练的发生具有非常重要的意义。  相似文献   

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