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1.
电路理论研究方法已经非常成熟.由于电路理论中的分析方法和基因网络结构功能很相似,因此可以采用电路理论中的分析方法,利用基因表达谱中的数据噪音,建立基因调控布尔网络.对基因和蛋白质,利用表达谱数据中的噪音建立基因调控布尔网络给出实例. 相似文献
2.
基因表达谱的肿瘤类型的准确判断对当前生物信息学的研究有重大意义.基因表达谱存在样本少、维数高、冗余基因和噪音多等特点,对癌症特征基因的提取方法的研究具有重要的意义.以结肠癌肿瘤基因表达谱数据作为研究对象,提出了结合使用基因选择和数据抽取的有效方法,剔除无关基因选出候选特征集,结合PCA(主元分析)获取低维投影空间中的模式特征,根据各个基因贡献率大小排序选取贡献率大的基因作为特征基因,进而利用支持向量机进行分类检测. 相似文献
3.
张明菊 《黄冈职业技术学院学报》2000,(1)
基因的表达调控有着严格的时空顺序。随着基因文库技术的发展和转基因作物的释放,对基因表达的调控机制的研究越来越受到科学界的重视。本文对转录和翻译水平的基因表达调控机制进行了较系统的综述。 相似文献
4.
生物信息学中的马尔可夫模型 总被引：1，自引：0，他引：1
随着人类基因组研究的不断发展,在世界各个（Genbank,SwissPort等）数据库中积累了大量蛋白质、核酸序列数据,如何分析和处理这些数据以获得更多的信息成为科学家们关心的核心问题,并由此产生了生物信息学。文章介绍了在生物信息学研究中常见的几种用于数据库搜索、序列比较、建立蛋白质模型、发现新基因等研究的马尔可夫模型。 相似文献
5.
随着基因芯片技术的快速发展以及其在基因表达分析等过程中的应用，产生了大量的基因表达谱数据，如何处理和分析这些数据并从中提取出有价值的生物学信息成为一个极为重要的课题，基因分类是进行基因数据处理的常用方法。本文首先利用主成分分析法(PCA)把基因的多个属性转化为少数几个综合属性，将基因表达谱数据映射成一个带权图，并将图论的最小生成树理论引入基因分类分析方法，然后设计了基于最小生成树的基因分类算法，理论分析和仿真结果表明了该算法的可行性和有效性。 相似文献
6.
在人类基因组计划的推动下,生物信息学得到了人们的广泛关注,并呈现出数量多、计算量大等鲜明特征,因此要求在生物信息学中采用计算机算法,以提高生物信息学处理问题的效率。以生物信息学中常用的计算机算法为切入点,进一步从基因表达数据分析、基因组序列信息分析、生物序列差异和相似性分析、遗传数据分析以及蛋白质结构与功能预测5个方面,论述了计算机算法在生物信息学中的典型应用。 相似文献
7.
本文从分子水平上讨论了真核生物基因结构及基因表达的调控,探讨了细胞内调控基因表达的各类顺式作用元件和反式作用因子,其中主要介绍边界元件绝缘子的调控机理。有关各种调控元件的研究对阐明细胞发育分化机理及某些发病机制的可能性也进行了讨论。 相似文献
8.
功能基因组学研究方法 总被引：5，自引：0，他引：5
随着后基因组时代的到来，基因组学的研究从结构基因组学转向了功能基因组学，基因功能的研究也从单一基因转向了大规模、批量分析。本文对功能基因组学研究方法进行了综述，主要包括：微阵列分析、基因表达系列分析、基因剔除和转基因技术、RNA 干涉、蛋白质组学和生物信息学等新方法。 相似文献
9.
为探讨乳腺癌患者大概率患有不孕症的情况,本研究应用生物信息学方法来研究乳腺癌标志物对卵丘细胞基因表达的影响。从NCBI的GEO数据库下载有关乳腺癌标志物的基因芯片数据库和卵丘细胞基因表达的基因芯片数据库。利用在线GEO2R筛选出2个数据库的差异基因,在EXCEL表格中进行筛选,对2个结果作韦恩图获得2个数据库差异基因的交集,利用DAVID6.8数据库对差异基因进行GO功能分析和KEGG通路分析,运用String-db数据库构建蛋白之间的相互作用图（PPI）,导入Cytoscape3.6.1软件获取关键靶基因。本文通过生物信息学方法从不同角度揭示有关乳腺癌标志物对卵丘细胞差异基因表达的影响,为人类乳腺癌患者生育能力的提高带来新思路。 相似文献
10.
抑癌基因Rb与P53的研究进展 总被引：1，自引：0，他引：1
符碧 《海南师范学院学报》2001,14(2):96-98
Rb和453均为抑癌基因，Rb全长大约200kb，定位于人13号染色体的13p14.1，它所编码的104kD磷酸蛋白参与细胞周期的调控，调控细胞增值。而P53窒长16－20kb，定位于人17号染色体的17p13.1，它所编码的53kD磷酸蛋白可通过调控CIPI基因表达而调控细胞生长。在很多肿瘤中已发现Rb和P53基因的突变与缺失，揭示了这两种基因与肿瘤发生有密切关系。 相似文献
11.
Garima Agarwal 《Resonance》2007,12(2):29-38
This article highlights an emerging field known as synthetic biology that envisions integrating designed circuits into living
organisms in order to instruct them to make logical decisions based on the prevailing intracellular and extracellular conditions
and produce a reliable behavior. The attempt is to design cells capable of sensing a bioweapon or an environmental pollutant,
activate its degradation pathway and perform bioremediation or carry out programmed cell death or synthesize complex biomaterials.
Within the living cell, a complex interplay of networks formed by genes and proteins mediate all cellular processes. The networks
in a system can be dissected into small regulatory gene circuit modules. Synthetic biology attempts to construct and assemble
such modules step by step, plug the modules together and modify them, in order to generate a desired behavior. The review
gives an insight into the creation of gene circuits and discusses the potential applications in the field of biotechnology,
medicine and environmental sciences.
Garima Agarwal is a research student in the Molecular Biophysics Unit, Indian Institute of Science. Her areas of research
interest include structural biology, bioinformatics and systems biology. 相似文献
12.
Veronica S. Flodin 《Science & Education》2017,26(1-2):141-170
The purpose of this study is to interpret and qualitatively characterise the content in some research articles and evaluate cases of possible difference in meanings of the gene concept used. Using a reformulation of Hirst’s criteria of forms of knowledge, articles from five different sub-disciplines in biology (transmission genetic, molecular biology, genomics, developmental biology and population genetics) were characterised according to knowledge project, methods used and conceptual contexts. Depending on knowledge project, the gene may be used as a location of recombination, a target of regulatory proteins, a carrier of regulatory sequences, a cause in organ formation or a basis for a genetic map. Methods used range from catching wild birds and dissecting beetle larvae to growing yeast cells in 94 small wells as well as mapping of recombinants, doing statistical calculations, immunoblotting analysis of protein levels, analysis of gene expression with PCR, immunostaining of embryos and automated constructions of multi-locus linkage maps. The succeeding conceptual contexts focused around concepts as meiosis and chromosome, DNA and regulation, cell fitness and production, development and organ formation, conservation and evolution. These contextual differences lead to certain content leaps in relation to different conceptual schemes. The analysis of the various uses of the gene concept shows how differences in methodologies and questions entail a concept that escapes single definitions and “drift around” in meanings. These findings make it important to ask how science might use concepts as tools of specific inquiries and to discuss possible consequences for biology education. 相似文献
13.
Integration of inquiry-based approaches into curriculum is transforming the way science is taught and studied in undergraduate classrooms. Incorporating quantitative reasoning and mathematical skills into authentic biology undergraduate research projects has been shown to benefit students in developing various skills necessary for future scientists and to attract students to science, technology, engineering, and mathematics disciplines. While large-scale data analysis became an essential part of modern biological research, students have few opportunities to engage in analysis of large biological data sets. RNA-seq analysis, a tool that allows precise measurement of the level of gene expression for all genes in a genome, revolutionized molecular biology and provides ample opportunities for engaging students in authentic research. We developed, implemented, and assessed a series of authentic research laboratory exercises incorporating a large data RNA-seq analysis into an introductory undergraduate classroom. Our laboratory series is focused on analyzing gene expression changes in response to abiotic stress in maize seedlings; however, it could be easily adapted to the analysis of any other biological system with available RNA-seq data. Objective and subjective assessment of student learning demonstrated gains in understanding important biological concepts and in skills related to the process of science. 相似文献
14.
15.
The focus of this Research Experience for Undergraduates (REU) project was on RNA secondary structure prediction by using a lattice walk approach. The lattice walk approach is a combinatorial and computational biology method used to enumerate possible secondary structures and predict RNA secondary structure from RNA sequences. The method uses discrete mathematical techniques and identifies specified base pairs as parameters. The goal of the REU was to introduce upper-level undergraduate students to the principles and challenges of interdisciplinary research in molecular biology and discrete mathematics. At the beginning of the project, students from the biology and mathematics departments of a mid-sized university received instruction on the role of secondary structure in the function of eukaryotic RNAs and RNA viruses, RNA related to combinatorics, and the National Center for Biotechnology Information resources. The student research projects focused on RNA secondary structure prediction on a regulatory region of the yellow fever virus RNA genome and on an untranslated region of an mRNA of a gene associated with the neurological disorder epilepsy. At the end of the project, the REU students gave poster and oral presentations, and they submitted written final project reports to the program director. The outcome of the REU was that the students gained transferable knowledge and skills in bioinformatics and an awareness of the applications of discrete mathematics to biological research problems. 相似文献
16.
传感器网络中的数据管理 总被引：1，自引：0，他引：1
无线传感器网络集成了传感器技术、无线通信技术、嵌入式计算技术、分布式信息管理技术和数据库技术,成为当前学术界和工业界关注的热点。传感器网络以数据为中心的特点,使得感知数据管理成为网络应用的核心技术。介绍了数据管理的概念和特点,分析了数据管理的研究内容和存在问题,并着重对当前国内国外已有的研究进展状况进行了总结和归纳。 相似文献
17.
EST技术在植物基因克隆和基因表达谱研究中的应用 总被引：2，自引：0，他引：2
表达序列标签是cDNA的部分序列，是通过对cDNA库进行大规模上次性测序而获得．对于植物研究而言，该技术已成为一种高效、经济的克隆重要植物基因的工具．而且，通过分析来自特定组织的EST库可以定量地估计该组织中各类基因的表达丰度．这种组织基因表达谱可用于阐明植物的代谢调控网络或植物对生物、非生物胁迫反应的信号转导途径．章以几个典型的实例阐释如何利用EST技术来克隆重要的植物经济性状基因及以此技术进行表达谱研究． 相似文献
18.
Treacy DJ Sankaran SM Gordon-Messer S Saly D Miller R Isaac SR Kosinski-Collins MS 《CBE life sciences education》2011,10(1):18-24
In introductory laboratory courses, many universities are turning from traditional laboratories with predictable outcomes to inquiry-inspired, project-based laboratory curricula. In these labs, students are allowed to design at least some portion of their own experiment and interpret new, undiscovered data. We have redesigned the introductory biology laboratory course at Brandeis University into a semester-long project-based laboratory that emphasizes concepts and contains an element of scientific inquiry. In this laboratory, students perform a site-directed mutagenesis experiment on the gene encoding human γD crystallin, a human eye lens protein implicated in cataracts, and assess the stability of their newly created protein with respect to wild-type crystallin. This laboratory utilizes basic techniques in molecular biology to emphasize the importance of connections between DNA and protein. This project lab has helped engage students in their own learning, has improved students' skills in critical thinking and analysis, and has promoted interest in basic research in biology. 相似文献
19.