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目的:从葱的根、茎、叶中分离内生真菌,并进行初步抗菌活性检测,得到具有高抗菌活性的菌株。方法:1.用PDA培养基分离葱的内生真菌;2.用查氏培养基进行发酵实验。3.进行滤纸片抑菌实验。结果:从葱中分离出的17株内生真菌,均具有抗菌活性,其中,抑菌圈直径大于1.0cm的高抗菌性菌株有11株,占总分离菌株数的64.71%。结论:葱内生真菌具有类似宿主植物葱一样的广泛抗菌性(分离得到的17株内生真菌均具有抗菌性),但由于宿主植物本身所具有的强抗菌性,使分离得到的菌株种属多样性较少。 相似文献
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《商洛学院学报》2019,(6):18-25
采用组织块分离法,从采自商洛的野生艾蒿根、茎和叶中分离到54株内生菌,其中35株细菌,19株真菌。以金黄色葡萄球菌、枯草芽孢杆菌、大肠杆菌为供试靶标菌,采用纸片法对分离的艾蒿内生细菌进行了拮抗活性的筛选,以棉花枯萎病菌、烟草赤星病菌、茄病镰孢霉为植物病原菌,用对峙法对内生真菌的拮抗活性进行了筛选。结果表明:分离到的内生细菌和真菌在野生艾蒿中的数量分布均表现为在根中最多,茎中次之,叶中最少。从内生细菌中筛选出了4株具有较强拮抗活性的菌株,分别是BS-6和BR-6菌株对枯草芽孢杆菌有较强拮抗作用,BR-9和BS-12菌株对大肠杆菌有较强拮抗作用;从内生真菌中筛选到2株对植物病原菌具有拮抗活性的菌株,分别是FS-8菌株对棉花枯萎病菌有较强的拮抗作用,FR-4菌株对茄病镰孢霉具有明显的拮抗作用。由此可见,野生艾蒿组织中内生菌含量较为丰富,而且其中存在对细菌和植物病原菌具有较强拮抗活性的菌株,其内生菌资源应进一步开发利用。 相似文献
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以改良PDA培养基为分离基础培养基,通过切片法从铁皮石斛(Dendrobium offidnale)菌根中分离培养后并用切取培养基分离得到纯化菌株.通过菌落形态观察,初步鉴定为4株内生真菌(Nj2010-1、Nj2010-2、Nj2010-3、Nj2010-4).实验表明材辩表面消毒条件和方法以及分离培养基的选用,均直接影响菌根真菌的分离纯化结果,及分离得到的内生菌的多样性.以75%酒精处理30秒0.1%升汞处理7min进行表面消毒,效果稳定,配合改良的PDA培养基分离内生菌,可以分离出较多的内生菌种类. 相似文献
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从柑橘的枝,叶,果实中分离培养出20株内生真菌,经形态特征鉴定它们分别为镰孢属（Fusarium sp．）、曲霉属（Aspergillus sp.）、毛霉属（Mucor sp．）、青霉属（Penicillium sp.）、葡萄孢属（Botrytis sp．）、盘多毛孢属（Pestalotia sp．）及3株不产孢子的未知菌株,各个菌属都具有较好的抗菌活性。 相似文献
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目的：将深海来源的16株真菌经发酵培养与活性筛选,获取活性菌株以供筛选药源活性产物。分别经真菌普通培养基和人工海水培养基发酵获得样品,采用MTT法测试抗肿瘤活性,纸片法测试抗真菌活性。结果：菌株中有3株经过普通发酵培养基发酵和4株经过海水培养基发酵的样品在100μg/mL浓度下对K562细胞的抑制率大于60%;抗真菌活性测试中,仅有菌株16-02-1的发酵样品对受试白色念珠菌ATCC 10231和土曲霉W-1均呈现一定的抑制活性。结论：深海来源真菌在不同培养基中发酵获得的样品,抗肿瘤活性各不相同,经过筛选获得高活性菌株为寻找药源活性产物提供了菌株。 相似文献
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本文对分离自莲瓣兰(Cymbidium lianpanTang et Wang)根内的5株内生真菌利用氮源的生理学特性进行研究,采用的方法是三点种植实验,培养5天,测量氮源培养基上内生真菌菌落直径,同时,进行液体培养5天,将菌丝过滤,烘干至恒重,计算菌丝干重。结果如下:这5株内生真菌利用氮源能力各不同;其中,属于木霉属的LP161菌株对多数氮源有较好的利用能力,但对尿素利用能力较差;并且,LP161菌株在硝酸钾液体培养基内生长最快,培养5天后菌丝干重达到0·78g/100ml。 相似文献
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从药用植物大黄藤中分离出内生放线菌26株.以金黄色葡萄球菌、炭疽病菌和链格孢菌为受试菌株,对其进行抗菌活性筛选.结果表明,共有14株内生放线菌对1株或1株以上的受试菌株有抗菌活性,其中平板抑菌圈直径大于20mm的菌株有1株(ADG11).研究结果表明,大黄藤内生放线菌具有良好的潜在应用价值,值得做进一步研究. 相似文献
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从峨眉山采集健康的一把伞南星(Arisaema erubescens(Wall)Schott)植株,采用稀释涂布平板法从植物的组织液中分离内生细菌,采用牛津杯法筛选对大肠杆菌(Escherichia coli)有拮抗作用的抗性菌株。研究结果表明,从一把伞南星中分离得到内生细菌23株,经过初筛和复筛共得到2株对大肠杆菌有较强抑菌活性的内生细菌:YC06、YC09,这两个菌株的发酵产物对大肠杆菌也有抑菌作用,抑菌圈直径均在13.0 mm以上。 相似文献
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从关白附（Aconitum Coreanum（Levl.）Rapaics）中分离出内生菌共29株,通过TLC检测筛选出2株可能产生生物碱类成分的内生菌.对筛选出的2株内生菌株的发酵培养物分别以纸片法和MTT法进行抑菌和细胞毒活性测定.实验结果显示,2个内生菌株发酵产物对金黄色葡萄球菌、大肠杆菌具有良好的抑菌作用,对肝癌细胞SMMC-7721、胃癌细胞SGC-7901具有良好的细胞毒活性.通过16S rDNA基因序列分析,2株内生菌分别被鉴定为类芽孢杆菌属和芽孢杆菌属细菌. 相似文献
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对药用植物云南萝芙木进行内生放线菌的分离.使用酒精和次氯酸钠对样品的根、茎、叶和果实进行表面消毒.采用两种分离培养基和三种分离方法并且分别添加重铬酸钾进行分离.共分离到17株内生放线菌. 相似文献
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Si-sheng Sun Xiao-mei Chen Shun-xing Guo 《Journal of Zhejiang University. Science. B》2014,15(2):109-115
Santalum album Linn. is an evergreen and hemi-parasitic tree, the heartwood-sandalwood of which was used during a long history in traditional Chinese medicine. Kuhnia rosmarinifolia Vent. is a good host for 1- or 2-year-old growing S. album. The interaction between S. album and K. rosmarinifolia is still little known. Many studies have been carried out on a number of plants for identification and diversity of endophytes. In this study, in total 25 taxa of endophytic fungi were isolated from the roots of S. album and the roots of K. rosmarinifolia. The most frequently isolated genera were Penicillium sp. 1 and Fusarium sp. 1 in the roots of S. album and K. rosmarinifolia, respectively. S. album is a root parasite of K. rosmarinifolia. The interesting result is that they apparently do not share the same endophytic fungi isolates. This study for the first time explored the content of endophytic fungi from S. album and K. rosmarinifolia, which provides important information for further studies. 相似文献
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The purpose of this study was to isolate and characterize endophytic fungi from the stem tissue which can produce fragrant
ingredients in Aquilaria sinensis (also called agarwood) to determine their antitumor and antimicrobial activities. Twenty-eight fungal endophytes were isolated
from agarwood by strict sterile sample preparation and were classified into 14 genera and 4 taxonomic classes (Sordariomycetes,
Dothideomycetes, Saccharomycetes, and Zygomycetes) based on molecular identification. Of the 28 isolates, 13 (46.4%) showed
antimicrobial activity against at least one of the test strains by the agar well diffusion method, and 23 isolates (82.1%)
displayed antitumor activity against at least one of five cancer cell lines by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide (MTT) assay. The diameters of inhibition zones of YNAS07, YNAS14, HNAS04, HNAS05, HNAS08, and HNAS11 were equal to
or higher than 14.0 mm against Staphylococcus aureus, Escherichia coli, Bacillus subtilis, B. subtilis, Aspergillus fumigatus, and B. subtilis, respectively. The inhibition rates of YNAS06, YNAS08, and HNAS06 were not less than 60% to 293-T, 293-T, and SKVO3 cells,
respectively. These results suggest that the endophytic fungi associated with agarwood will provide us with not only useful
micro-ecological information, but also potential antimicrobial and antitumor agents. 相似文献
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Liu Zhijing Xu Cong Tian Ran Wang Wan Ma Jiage Gu Liya Liu Fei Jiang Zhanmei Hou Juncai 《Journal of Zhejiang University. Science. B》2021,22(7):533-547
Lactic acid bacteria(LAB) are a representative probiotic. As the dominant flora in the human intestinal tract, LAB can regulate the balance of human intestinal flora and improve host health. The purpose of this study was to isolate and screen LAB that are well suited to the intestinal characteristics of the Chinese population, with excellent probiotics and high antibacterial activity. After 16 S ribosomal RNA(rRNA) homology and phylogenetic tree analysis, potential probiotics were tested for their antibacterial activity, resistance to artificial gastrointestinal fluid and drugs, surface hydrophobicity, and safety.Three strains of LAB with acid resistance, bile salt resistance, epithelial cell adhesion, and no multidrug resistance were selected: Lactobacillus salivarius, Leuconostoc lactis, and Lactobacillus paracasei. Analysis of the antibacterial active substances in the three strains and their fermentation broths revealed that the main antibacterial substances of L. lactis were organic acids, whereas those of L. salivarius and L. paracasei were organic acids and bacteriocins with broad-spectrum antibacterial activity. These three strains of probiotic LAB with high antibacterial activity were identified as bacterial resources that could potentially be used to develop probiotic preparations for the prevention and treatment of intestinal diseases caused by intestinal pathogens. 相似文献