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1.
Membrane lipid and protein composition was compared in erythrocytes from iron deficiency anemia (IDA) and heterozygous beta
thalassemia patients. The study was planned to correlate the influence of iron deficiency with the intrinsic defect of the
heterozygous condition on the membrane structural integrity as well as to investigate whether there are differences in membrane
changes between the two conditions. Results indicate high levels of saturated fatty acids and low unsaturated fatty acids
in both disorders although arachidonic acid and the unsaturation index were lower in heterozygous thalassemia than IDA. Nevertheless,
neither of the conditions provoked any alterations in membrane protein or glycophorin suggesting alterations in the lipid
moiety only. Present findings indicate that irrespective to the etiology, both, iron deficiency and the heterozygous condition
show a common pattern of lipid derangement, which may in turn result in increased membrane rigidity and decreased cellular
deformability. 相似文献
2.
De Manisha D. Banerjee G. Talukdar D. K. Bhattacharya 《Indian journal of clinical biochemistry : IJCB》1999,14(2):207-212
The aim of the present work is to understand the lipid peroxidation of RBC membrane and the spectrin protein content of RBC
membrane cytoskeleton of thalassaemic carrier state (trait) of β and hemoglobin E variant (HbE). We have measured the hemoglobin
(Hb), malondialdehyde (MDA) and spectrin content of RBC membrane of thalassaemic carrier. The spectrin content (α and β band)
of both β and HbE carrier was not changed than normal individuals. However, lipid peroxidation of RBC membrane was significantly
increased in both β and HbE trait, and Hb level was also decreased in thalassaemic carrier. It may be assumed that oxidative
damage by excess lipid peroxidation may have no role on irreversible membrane damage in β thalassaemia and HbE thalassaemia
carrier. 相似文献
3.
Malaria infection is known to cause severe hemolysis due to production of abnormal RBCs and enhanced RBC destruction through apoptosis. Infected RBC lysis exposes uninfected RBC to the large amount of pro-oxidant molecules such as methemoglobin. Methemoglobin (MetHb) exposure dose dependently makes RBCs susceptible to osmotic stress and causes hemolysis. MetHb mediated oxidative stress in RBC correlated well with osmotic fragility and hemolysis. Interestingly, a reactive oxygen species (ROS) spike at 15 min was responsible for the observed effects on RBC cells. Two natural antioxidants N-acetyl cysteine and mannitol protected the RBC from MetHb-mediated defects, which clearly indicated involvement of oxidative stress in the process. MetHb due to its pseudo-peroxidase activity produces ROS in the external microenvironment. Therefore, classical peroxidase inhibitors were tested to probe peroxidase activity mediated ROS production with defects in RBCs. Clotrimazole (CLT), which irreversibly inactivates the MetHb (CLT-MetHb) and abolishes peroxidase activity, did not produce significant ROS outside RBC and was inefficient to cause osmotic fragility and hemolysis. Hence, initiating a chain reaction, MetHb released from ruptured RBC produces significant ROS in the external microenvironment to make RBC membrane leaky and enhanced hemolysis. Together data presented in the current work explored the role of MetHb in accelerated humorless during malaria which could be responsible for severe outcomes of pathological disorders. 相似文献
4.
Manisha De N. Banerjee G. Talukdar D. K. Bhattacharya 《Indian journal of clinical biochemistry : IJCB》2000,15(1):56-59
The aim of the present work is to understand the effect of low dose of hydroxyurea (HU) therapy on oxidative damage of RBC
membrane in non-transfused Eβ thalassaemia. HU was administered at a dose of 30 mg/kg/day for 90 consecutive days. The percentage
of spectrin and the level of malondialdehyde (MDA), fetal hemoglobin (HbF), hemoglobin (Hb) and packed cell volume (PCV) were
measured. The HbF level was significantly increased after 90 days of HU therapy. MDA level of RBC membrane was decreased.
There was no change in PCV, Hb and spectrin content of RBC membrane after HU therapy for 90 days in Eβ thalassaemia. Increment
of HbF in HU treated Eβ patient may have some role on the correction of oxidative damage of RBC membrane by inhibiting further
degradation of spectrin and by decreasing lipid peroxidation of red cell membrane. 相似文献
5.
Gauri S. Vahalkar Vijaya A. Haldankar 《Indian journal of clinical biochemistry : IJCB》2008,23(3):223-226
Glyco-oxidation is considered as a source of permanent, cumulative, oxidative damage to long lived proteins in ageing and
in diabetes. Although RBC depends solely on glucose for energy purpose, hyperglycemic state glycosylates hemoglobin, creates
oxidative stress and puts the cellular components at risk. In view of this, RBC membrane composition was analyzed in diabetic
patients. The results were compared with healthy age and sex matched control groups. When RBC membrane components such as
protein, sialic acid, phospholipids and cholesterol were determined in insulin dependent diabetes mellitus, a significant
rise in phospholipids and cholesterol and significant fall in sialic acid and protein content was noted. RBC membrane composition
showed pronounced alterations in insulin dependent diabetes mellitus. These changes were accompanied by higher levels of lipid
peroxidation products like Malondialdehyde. 相似文献
6.
Ethanol-induced liver injury may be linked, at least partly, to an oxidative stress resulting from increased free radical
production and/or decreased antioxidant defence. Distinguishing alcoholic and non-alcoholic liver disease has important implications.
This study looked at the possible changes between alcoholic and non-alcoholic liver diseases by examining the presence of
oxidative damage, as monitored by several parameters relating to oxidative stress. Lipid peroxides concentration, superoxide
dismutase activity and glutathione S-transferase activity increased, where as glutathione content, glutathione peroxidase
activity and glutathione reductase activity decreased among the tested subjects in comparison to normal healthy group. Determination
of these parameters may be valuable in the evaluation of liver disease. However, oxidative stress related enzymes and non-enzymes
can not be utilized as a marker for alcoholic liver diseases, as these parameters responded in the same way after liver is
damaged irrespective of their cause. Their level may help in determining the degree of liver damage. Degree of oxidative injury
was similar in patients with non-alcoholic liver disease and in moderate drinkers; while significantly higher in heavy drinkers.
The differences between the groups might be based on the type of liver pathological condition rather than its etiology (i.e.
alcohol and non alcohol related causes). 相似文献
7.
Giovanna Tomaiuolo 《Biomicrofluidics》2014,8(5)
Red blood cells (RBCs) possess a unique capacity for undergoing cellular deformation to navigate
across various human microcirculation vessels, enabling them to pass through capillaries that are
smaller than their diameter and to carry out their role as gas carriers between blood and tissues.
Since there is growing evidence that red blood cell deformability is impaired in some pathological
conditions, measurement of RBC deformability has been the focus of numerous studies over the past
decades. Nevertheless, reports on healthy and pathological RBCs are currently limited and, in many
cases, are not expressed in terms of well-defined cell membrane parameters such as elasticity and
viscosity. Hence, it is often difficult to integrate these results into the basic understanding of
RBC behaviour, as well as into clinical applications. The aim of this review is to summarize
currently available reports on RBC deformability and to highlight its association with various human
diseases such as hereditary disorders (e.g., spherocytosis, elliptocytosis, ovalocytosis, and
stomatocytosis), metabolic disorders (e.g., diabetes, hypercholesterolemia, obesity), adenosine
triphosphate-induced membrane changes, oxidative stress, and paroxysmal nocturnal hemoglobinuria.
Microfluidic techniques have been identified as the key to develop state-of-the-art dynamic
experimental models for elucidating the significance of RBC membrane alterations in pathological
conditions and the role that such alterations play in the microvasculature flow dynamics. 相似文献
8.
Maternal and fetal indicators of oxidative stress during intrauterine growth retardation (IUGR) 总被引:1,自引:0,他引:1
Ullas Kamath Guruprasad Rao Shobha U Kamath Lavanya Rai 《Indian journal of clinical biochemistry : IJCB》2006,21(1):111-115
The present study demonstrates the possibility of increased lipid peroxidation and protein oxidation in both maternal and
fetal erythrocytes as markers of oxygen radical activity during intrauterine growth retardation. The erythrocyte MDA levels
were significantly elevated in mothers of IUGR babies when compared to controls (p<0.01). The endogenous protein damage due
to oxidative stress was significantly higher in IUGR mothers when compared to controls (p<0.05). Similarly the proteolytic
activity in erythrocyte lysates against oxidatively damaged hemoglobin was significantly increased in mothers of IUGR babies
compared to controls (p<0.001).
In fetuses born with IUGR, both lipid peroxidation and proteolytic activity were significantly increased when compared to
normal newborns (p<0.01).
The result of this study indicates that oxidative stress was induced both in IUGR babies and their mothers which is manifested
as increased lipid peroxidation and protein oxidant damage. 相似文献
9.
Guruprasad Rao Ullas Kamath Chaerkadi Raghothama K. Sujatha Pradeep Pragna Rao 《Indian journal of clinical biochemistry : IJCB》2003,18(2):80-86
The present study demonstrates the incidence of increased lipid peroxidation and protein oxidation in both maternal and fetal
erythrocytes as markers of oxygen radical activity in different complications of pregnancy. In fetuses born after premature
rupture of membranes, lipid peroxidation was significantly elevated as indicated by increased malondialdehyde levels (p<0.05)
as compared to controls. Proteolytic activity in the erythrocytes of mothers in this group was also significantly high (p<0.01).
In patients delivered by lower segment cesarian section, lipid peroxidation and proteolytic activity in maternal erythrocytes
were significantly high (p<0.05 and p<0.001 respectively). In patients with prolonged second stage of labour, lipid peroxidation
and proteolytic activity in maternal erythrocytes was significantly higher than in controls (p<0.001 and p<0.05 respectively).
In this group, endogenous protein damage due to oxidative stress was significantly high both in the mother and the fetus (p<0.001
and p<0.05 respectively). 相似文献
10.
Tiwari AK Mahdi AA Zahra F Chandyan S Srivastava VK Negi MP 《Indian journal of clinical biochemistry : IJCB》2010,25(4):411-418
The present study was conducted to investigate the oxidant–antioxidant status in iron deficient pregnant anemic women. One
hundred thirty pregnant women with iron deficiency anemia (IDA) were divided into three groups, namely mild (50), moderate
(50) and severe (30) anemic along with pregnant healthy women as controls (50). The complete blood count, plasma lipid peroxidation
products, enzymatic and non-enzymatic antioxidants were measured according to respective protocols. The levels of complete
blood count, iron, ferritin along with antioxidant enzymes namely catalase, superoxide dismutase, glutathione peroxidase,
glutathione reductase and reduced glutathione were significantly reduced in all IDA groups. However, the level of oxidized
glutathione, lipid peroxides, protein carbonyls, conjugated dienes were found significantly increased in all anemic patients.
Antioxidant vitamins, namely C, E and A were also found significantly decreased in IDA patients. On the basis of our results,
it may be concluded that IDA tends to increase the pro-oxidant components, which may result in various complications including
peroxidation of vital body molecules resulting in increased risk for pregnant women as well as fetus. 相似文献
11.
S. B. Patil M. V. Kodliwadmath Sheela M. Kodliwadmath 《Indian journal of clinical biochemistry : IJCB》2007,22(1):135-137
Pregnancy is a physiological state accompanied by a high-energy demand and an increased oxygen requirement. Augmented levels
of oxidative stress would be expected because of the increased intake and utilization of oxygen. Evidence of increased oxidative
stress in normal pregnancy in comparison with nonpregnant controls was estimated by measuring Thiobarbituric acid reactive
substance (TBARS), enzymatic antioxidants like Superoxide dismutase, Glutathione peroxidase, Glutathione reductase and catalase.
It was observed that pregnant women were more susceptible to oxidative damage than non-pregnants as indicated by increased
TBARS and decreased antioxidants. 相似文献
12.
Devika Tayal Binita Goswami S. K. Patra Reva Tripathi Alka Khaneja 《Indian journal of clinical biochemistry : IJCB》2014,29(2):139-144
Preeclampsia is a multisystem disorder associated with maternal hypertension, placental abnormalities and adverse fetal outcomes. The various pathways involved in its etiology include endothelial dysfunction, inflammatory milieu, lipid peroxidation and immunological imbalance. The present study was conducted to evaluate the causative and predictive role of nitric oxide, lipid peroxidation end products (MDA) and inflammatory cytokines (IL-6, TNF-α) in clinical presentation, severity and fetal outcome in preeclampsia. The study population was divided into 3 groups- Non- pregnant females comprising the control population; G1 and G2 groups included normal pregnant and pregnant females with preeclampsia with 50 patients in each group. Nitric Oxide and MDA levels were found to be highest in the preeclamptic patients as compared to other two groups. ROC curve analysis shows the superiority of the inflammatory markers as determinants of severity of preeclampsia which suggests the emerging role of pro inflammatory markers in the various pathological changes in preeclampsia. TNF-α emerged as the best marker in multivariate analysis and thus, has the potential for being used as a marker for PIH. Our study illustrates the multifactorial etiology of preeclampsia involving oxidative stress, proinflammatory milieu and endothelial dysfunction. 相似文献
13.
Susceptibility of Schizophrenic patients to lipid peroxidation relative to healthy control subjects was investigated by measuring
the malondialdehyde (MDA) levels in plasma. The main finding was that Schizophrenic patients were more susceptible than control
subjects to oxidative damage as evident from increased MDA levels in plasma. Antioxidant levels are also depleted in Schizophrenic
patients when compared to normal subjects as evident from decreased levels of vitamins E and C in the plasma. Impaired antioxidant
defense and increased lipid peroxidation suggests that treatment with antioxidants (Vitamin E, Vitamin C, beta carotene) at
the initial stages of illness may prevent further oxidative injury and deterioration of associated neurological deficits in
Schizophrenia. 相似文献
14.
Pushpa Durgawale P. S. Shukla Sonali Mishra 《Indian journal of clinical biochemistry : IJCB》1999,14(2):241-244
An unexpected increase in erythrocyte osmotic resistance during viral hepatitis in two patients prompted study of effects
of hepatitis (in Karad) on osmotic resistance. The test was performed by placing erythrocytes in saline solutions of decreasing
osmolarity and osmotic fragility defined in terms of the saline concentration at which hemolysis begins. Study included 44
infected subjects (acute viral hepatitis non-A non-B). All forty four infected subjects showed abnormal results when compared
to normal subjects. increased erythrocyte osmotic resistance i.e. hemolysis begins between 0.45% to 0.40% of NaCl and is completed
between 0.25% to 0.20% NaCl, whereas in normal subjects. hemolysis begins between 0.50% to 0.45% NaCl and is completed between
0.35% to 0.30% NaCl. Osmotic resistance was observed with increase in total bilirubin (mean±SD) (4.6 ±3.6), direct bilirubin
(3.0±2.5), SGOT (58.8±55.5) SGPT (114.2±150.3) activity. Physiologic shifts in erythrocyte osmotic resistance may be due to
changes in membrane lipid ratio. 相似文献
15.
Manzoor Ahmad Nida Suhail Tariq Mansoor Naheed Banu Shamshad Ahmad 《Indian journal of clinical biochemistry : IJCB》2012,27(4):385-388
In the present study, oxidative stress and lymphocytic DNA damage in both pre-op and post-op benign prostrate hyperplasia (BPH) patients with age >50 years was evaluated and compared with normal healthy subjects (controls- without any evidence of disease) of the same sex and age group. From December 2007 to November 2009, oxidative stress in 45 BPH patients were evaluated both before (pre-op patients) and after 7 days of surgery (post-op patients) in terms of measurements of plasma levels of (1) various anti-oxidative enzymes, (2) non-enzymatic antioxidants and (3) malondialdehyde which is a product of lipid peroxidation. The lymphocyte DNA damage was also evaluated by single cell alkaline gel electrophoresis in terms of tail length migration in these patients. These values were compared with their respective control subjects of similar sex and age group. The activities of antioxidant enzymes and the levels of antioxidant, reduced glutathione were found significantly decreased (p < 0.05) in serum samples of pre-operative group of BPH patients as compared to the controls. These altered parameters increased significantly (p < 0.05) and returned to their near normal control values, but not up to baseline values, in post operative patients i.e. after the cancer load was decreased by surgery. Lymphocytic DNA damage was found to be significantly increased in pre-op group as compared to controls and was reduced after surgery in post-op group. The present study therefore, shows significantly increased levels of oxidative stress and DNA damage in BPH patients which were reduced after removal of tumour load. Thus oxidative damage plays an important role in prostate tumourogenesis and timely management of oxidative stress can be of importance in preventing the occurrence of BPH. 相似文献
16.
T. A. Ajith 《Indian journal of clinical biochemistry : IJCB》2010,25(1):67-73
Iron is an essential nutrient for a number of cellular activities. However, excess cellular iron can be toxic by producing
reactive oxygen species (ROS) such as superoxide anion (O2−) and hydroxyl radical (HO·) that damage proteins, lipids and DNA. Mutagenic and genotoxic end products of lipid peroxidation can induce the decline
of mitochondrial respiration and are associated with various human ailments including aging, neurodegenerative disorders,
cancer etc. Zingiber officinale Roscoe (ginger) is a widely used spice around the world. The protective effect of aqueous
ethanol extract of Z. officinale against ROS-induced in vitro lipid peroxidation and DNA damage was evaluated in this study.
The lipid peroxidation was induced by hydroxyl radical generated from Fenton’s reaction in rat liver and brain homogenates
and mitochondrial fraction (isolated from rat liver). The DNA protection was evaluated using H2O2-induced changes in pBR-322 plasmid and Fenton reaction-induced DNA fragmentation in rat liver. The results indicated that
Z. officinale significantly (P<0.001) protected the lipid peroxidation in all the tissue homogenate/mitochondria. The extract
at 2 and 0.5 mg/ml could protect 92 % of the lipid peroxidation in brain homogenate and liver mitochondria respectively. The
percent inhibition of lipid peroxidation at 1mg/ml of Z. officinale in the liver homogenate was 94 %. However, the extract
could partially alleviate the DNA damage. The protective mechanism can be correlated to the radical scavenging property of
Z. officinale. The results of the study suggest the possible nutraceutical role of Z. officinale against the oxidative stress
induced human ailments. 相似文献
17.
Al-Orf SM 《Indian journal of clinical biochemistry : IJCB》2011,26(2):154-160
In this study it was planned to investigate the effect of oxidized phosphatidylcholine (derived from egg) feeding on lipid
peroxidation of different tissues in rats. Male Wistar albino rats were fed oxidized and unoxidized phosphatidylcholine for
2 and 4 weeks, respectively. During the period of study food intake and body weights of animals increased gradually. Animals
fed oxidized phosphatidylcholine for 2 and 4 weeks showed 33 and 15% spontaneous hemolysis of red blood cells in vitro. Under
identical experimental conditions animals given unoxidized phosphatidylcholine showed 14.5 and 13.4% hemolysis for 2 and 4
week’s period, respectively. Thiobarbituric acid reactive substances (TBARS) level in thymus, spleen, kidney, heart, liver
and lung significantly increased in rats given oxidized phosphatidylcholine as compared to unoxidized group. Furthermore,
in oxidized phosphatidylcholine group TBARS values in kidney, liver and lungs continued to rise for 4 weeks of treatment while
TBARS level in heart, spleen and thymus was found to be decreased at the end of 4 weeks of oxidized phosphatidylcholine feeding.
Plasma triacylglycerol and cholesterol was found to increase in rats who had received oxidized phosphatidylcholine for 2 weeks.
These findings suggest that excess and persistent intake of oxidized phosphatidylcholine can cause significant damage to organs. 相似文献
18.
G. L. Soni A. K. Bansal N. Malhotra 《Indian journal of clinical biochemistry : IJCB》1992,7(2):196-198
Lipid peroxidation and hemolysis in humand red blood cells (RBCs) increased on their exposure to increasing concentrations of lead acetate (0.01–1.0mM). However pretreatment of RBCs with ascorbic acid (2mM and 4mM) significantly reduced the effect of lead acetate on lipid peroxidation and hemolysis in human RBCs. 相似文献
19.
D. Vijayakumar K. Suresh S. Manoharan Ph.D. 《Indian journal of clinical biochemistry : IJCB》2006,21(1):105-105
The main objective of the study was to assess the oxidative stress in plasma and erythrocytes of rheumatoid arthritis patients
by measuring the levels of thiobarbituric acid reactive substances (TBARS), non-enzymatic antioxidants (vitamin E, C and reduced
glutathione) and enzymatic antioxidants [superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSHPx)]. This
study has been conducted on twenty-two adult female rheumatoid arthritis patients and an equal number of healthy subjects.
Elevated lipid peroxidation and multidirectional changes in the antioxidant defence system were noticed in patients with rheumatoid
arthritis. The enhanced lipid peroxidation accompanied by disturbance in antioxidant status indicates that rheumatoid arthritis
patients are more prone to free radical mediated oxidative damage. 相似文献
20.
Rahul A. Ghone K. M. Kumbar A. N. Suryakar R. V. Katkam N. G. Joshi 《Indian journal of clinical biochemistry : IJCB》2008,23(4):337-340
Repeated blood transfusion in beta thalassemia major patients may lead to peroxidative tissue injury by secondary iron overload.
In the present study, 72 children with beta thalassemia major were included. Serum levels of total lipid peroxides, Iron,
Total Iron Binding Capacity, Copper, Zinc, Vitamin E, plasma Total Antioxidant Capacity, activity of Erythrocyte Superoxide
Dismutase, were measured. The findings were compared with 72 age matched healthy controls irrespective of sex. A significant
increase in the levels of lipid peroxide and Iron (p<0.001), whereas, significant decrease in the levels of vitamin-E, Total
Antioxidant Capacity and Total Iron Binding Capacity (p<0.001) was observed. Serum Zinc was significantly increased (p<0.001)
with significant decrease in the levels of copper (p<0.001). Non Significant increase in the activity of Erythrocyte Superoxide
Dismutase (p>0.05) was found in the patients when compared with controls. This suggest that oxidative stress and reduced antioxidant
defense mechanism play an important role in pathogenesis of beta thalassemia major. 相似文献