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INTRODUCTION Gene duplication provides a main resource of new genes in genomes (Ohno, 1970; Brown, 1999). Sequences of two paralogous genes from a duplication event will become different from each other along with evolutionary processes. And the difference in sequences caused by substitution, deletion, insertion of nucleotide acid will cause maximal sequence lengths of exact match (MALE) between paralogous members from a gene family to become shorter during evolution. As for example, o… 相似文献
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根据拟南芥Flowering Locus T（FT）基因和水稻Heading date 3a（Hd3a）基因的序列设计引物,通过PCR扩增的方法分别从麻竹和龙竹基因组DNA中各得到长为334bp的DNA片段.经BLAST检索、基因结构分析和编码蛋白功能域预测等分析,初步确定所得DNA片段可能是麻竹和龙竹中的FT同源基因的部分序列. 相似文献
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Two ancient rounds of polyploidy in rice genome 总被引：10，自引：0，他引：10
An ancient genome duplication (PPP1) that predates divergence ofthe cereals has recently been recognized. We report here another potentially older large-scale duplication (PPP2) event that predates monocot-dicot divergence in the genome of rice(Oryza saliva L.), as inferred from the age distribution of pairs of duplicate genes based on recent genome data for rice. Our results suggest that paleopolyploidy was widespread and played an important role in the evolution of rice. 相似文献
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An ancient genome duplication (PPP1) that predates divergence of the cereals has recently been recognized. We report here another potentially older large-scale duplication (PPP2) event that predates monocot-dicot divergence in 相似文献
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INTRODUCTION Cytokinins play a major role in many differentdevelopmental and physiological plant processes,such as cell division, regulation of root and shootgrowth and branching, chloroplast development,leaf senescence, stress response and pathogen re-sistance (Mok and Mok, 2001). Significant ad-vances have been achieved in our understanding ofcytokinin signaling, providing important insightsinto the molecular partners involved (Hutchisonand Kieber, 2002; Sheen, 2002). It had been… 相似文献
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AtCRE1 is known to be a cytokinin receptor in Arabidopsis. The AtCRE1 protein contains CHASE domain at the N-terminal part, followed by a transmitter (histidine kinase) domain and two receiver domains. The N-terminal CHASE domain of AtCRE1 contains putative recognition sites for cytokinin. Five CHASE domains containing proteins were found in rice, OsCRL1a, OsCRL1b, OsCRL2, OsCRL3, and OsCRL4. OsCRL1a, OsCRL1b, OsCRL2 and OsCRL3 contain the four domains existing in CRE1, whereas OsCRL4 only contains the CHASE domain and a putative Ser/Thr protein kinase domain. The authors cloned the encoding gene OsCRL4 and found that it represents a new member of the cytokinin receptor protein in rice. 相似文献
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Production of bacterial blight resistant lines from somatic hybridization between Oryza sativa L. and Oryza meyeriana L. 总被引：2，自引：0，他引：2
Novel bacterial blight (BB) resistance gene(s) for rice was (were) introduced into a cultivated japonica rice variety Oryza sativa (cv. 8411), via somatic hybridization using the wild rice Oryza meyeriana as the donor of the resistance gene(s). Twenty-nine progenies of somatically hybridized plants were obtained. Seven somatically hybridized plants and their parents were used for AFLP (amplified fragment length polymorphism) analysis using 8 primer pairs. Results confirmed that these plants were somatic hybrids containing the characteristic bands of both parents. The morphology of the regenerated rice showed characters of both O. sativa and O. meyeriana. Two somatic hybrids showed highest BB resistance and the other 8 plants showed moderate resistance. The new germplasms with highest resistance have been used in the rice breeding program for the improvement of bacterial blight resistance. 相似文献
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Novel bacterial blight (BB) resistance gene(s) for rice was (were) introduced into a cultivated japonica rice variety Oryza sativa (cv. 8411), via somatic hybridization using the wild rice Oryza meyeriana as the donor of the resistance gene(s). Twenty-nine progenies of somatically hybridized plants were obtained. Seven somatically hybridized plants and their parents were used for AFLP (amplified fragment length polymorphism) analysis using 8 primer pairs. Results confirmed that these plants were somatic hybrids containing the characteristic bands of both parents. The morphology of the regenerated rice showed characters of both O. sativa and O. meyeriana. Two somatic hybrids showed highest BB resistance and the other 8 plants showed moderate resistance. The new germplasms with highest resistance have been used in the rice bfeeding program for the improvement of bacterial blight resistance. 相似文献
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孙亮先 《泉州师范学院学报》2006,24(2):83-88
基因表达系列分析(Serial analysis of gene expression,SAGE)是功能基因组学研究中的一项功能强大的工具.对水稻幼苗SAGE文库的构建进行了探索试验,其主要操作步骤为:以磁珠法提取PolyA mRNA用于合成双链cDNA,再经过一系列的酶切、加接头序列、连接、PCR扩增,获得到26-bp的水稻双标签体,再将26-bp Ditag连接成标签链接体,并将其克隆进pZero-1载体中用于测序.DNA测序结果证明获得了水稻的SAGE标签.试验的各个中间步骤的结果均经过PCR验证.yh 相似文献
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Insertion mutagenesis has become one of the most popular methods for gene functions analysis. Here we report a two-elementAc/Ds transposon system containing enhancer trap and gene trap for gene tagging in rice. The excision ofDs element was examined by PCR amplification. The excision frequency ofDs element varied from 0% to 40% among 20 F2 populations derived from 11 differentDs parents. Southern blot analysis revealed that more than 70% of excisedDs elements reinserted into rice genome and above 70% of the reinsertedDs elements were located at different positions of the chromosome in rice. The result of histochemical GUS analysis indicated
that 28% of enhancer trap and 22% of gene trap tagging plants displayed GUS activity in leaves, roots, flowers or seeds. The
GUS positive lines will be useful for identifying gene function in rice.
Project supported by the Hi-Tech Research and Development Program (863) of China (No. 2002AA2Z1003) 相似文献
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Cheng-qi Yan Kai-xian Qian Gang-ping Xue Zhong-chang Wu Yue-lei Chen Qiu-sheng Yan Xue-qing Zhang Ping Wu 《浙江大学学报(A卷英文版)》2004,5(10):1199-1205
Novel bacterial blight (BB) resistance gene(s) for rice was (were) introduced into a cultivated japonica rice variety Oryza sativa (cv. 8411), via somatic hybridization using the wild rice Oryza meyeriana as the donor of the resistance gene(s). Twenty-nine progenies of somatically hybridized plants were obtained. Seven somatically
hybridized plants and their parents were used for AFLP (amplified fragment length polymorphism) analysis using 8 primer pairs.
Results confirmed that these plants were somatic hybrids containing the characteristic bands of both parents. The morphology
of the regenerated rice showed characters of both O. sativa and O. meyeriana. Two somatic hybrids showed highest BB resistance and the other 8 plants showed moderate resistance. The new germplasms with
highest resistance have been used in the rice breeding program for the improvement of bacterial blight resistance.
Project (No. 98001) supported by Ningbo Agriculture Key Scientific Research Foundation, China 相似文献
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INTRODUCTIONAfterthewholericegenomewassequencedsuccessfully,moreandmoregenescanbepredictedwithdifferentsoftwaresandannotationsystem.However,thefunctionsofpredictedgeneshavetobefurtheridentifiedbybiologicalanalysis.Oneofthemostpowerfulmethodsassigningfunctiontogeneisthroughinsertionmutagenesiswithtrans-posableelementasDNAsequencetag.Ac/Dstrans-posonsystemhasbecomeaverypopulartoolforgenetaggingandfunctionalgenomicsinvariousplantspecies(Altmannetal.,1995;Chinetal.,1999;Enokietal.,1999;Gre… 相似文献
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Insertion mutagenesis has become one of the most popular methods for gene functions analysis. Here we report a two-element Ac/Ds transposon system containing enhancer trap and gene trap for gene tagging in rice. The excision of Ds element was examined by PCR amplification. The excision frequency of Ds element varied from 0% to 40% among 20 F2 populations derived from 11 different Ds parents. Southern blot analysis revealed that more than 70% of excised Ds elements reinserted into rice genome and above 70% of the reinserted Ds elements were located at different positions of the chromosome in rice. The result of histochemical GUS analysis indicated that 28% of enhancer trap and 22% of gene trap tagging plants displayed GUS activity in leaves, roots, flowers or seeds. The GUS positive lines will be useful for identifying gene function in rice. 相似文献
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Xin-chun Zhang Xin Yu Hui-juan Zhang Feng-ming Song 《Journal of Zhejiang University. Science. B》2009,10(10):731-739
We cloned and characterized a rice gene OsBIABPI encoding an AMP-binding protein. The full-length cDNA of OsBIABP1 is 1912-bp long and is predicted to encode a 558-aa protein. OsBIABP1 contains a typical AMP-binding signature motif and shows high similarity to members of AMP-binding protein family. OsBIABP1 is expressed in stems, leaves and flowers of rice plants, but is not expressed, or expressed at a very low level, in rice roots. The expression of OsBIABP1 was induced by some defense-related signal molecules, e.g., salicylic acid (SA), benzothiadiazole, jasmonic acid (JA), and 1-amino cyclopropane-1-carboxylic acid, which mediate SA-and JA/ethylene (ET)-dependent defense signaling pathways, respectively. Furthermore, the expression of OsBIABP1 is activated by the infection of Magnaporthe oryzae, and the induced expression is quicker and stronger during early stages of pathogenesis in incompatible interaction than that in compatible interaction between rice and M. oryzae. Our results suggest that OsBIABP1 may be a defense-related AMP-binding protein that is involved in the regulation of defense re-sponse through SA and/or JA/ET signaling pathways. 相似文献
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Comparative mapping of QTLs for Al tolerance in rice and identification of positional Al-induced genes 总被引：2，自引：0，他引：2
INTRODUCTION Aluminum (Al) toxicity is one of the mostimportant yield-limiting factors for crop grown onacid upland and lowland acid sulphate soils (IRRI,1978). Al toxicity results in a reduced and damagedroot system, which in turn causes the affectedplants to be susceptible to drought stress and min-eral nutrient deficiencies (Foy, 1988). The physio-logical and biochemical mechanisms of the toxiceffect of Al on root elongation had been extensivelyinvestigated (Matsumoto, 2000). T… 相似文献