共查询到20条相似文献，搜索用时 78 毫秒
1.
目的:研究与胰岛素抵抗有关的三个基因的多态性GPR10(G-protein-coupled re-ceptor 10)(-62 G/A)、FOXC2(Forkhead box C2)(-512 C/T)、PGC1(Peroxisome proliferatoractivated receptorγcoactivator-1)(Thr394Thr)与2型糖尿病相关性.方法:利用等位基因专一性PCR的方法,对来自上海地区的正常人和肥胖的2型糖尿病患者进行基因分型,并通过X2比较基因型频率及等位基因频率在两组人群中是否存在显著性差异.结果:GPR10基因-62G/A多态性基因型频率在糖尿病人群和正常人群中的分布分别为:糖尿病人AA 14、AG 39、GG 34;正常人AA 2、AG 44、GG 42,具有显著性差异,等位基因频率分布为:糖尿病人A 67 G107、正常人A48、G 128,也有显著性差异.结论:GPR10基因-62G/A多态性可能与2型糖尿病相关,但该结果仍有待于进一步扩大样本量进行验证. 相似文献
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目的:通过检测张家口地区45例健康女性和34例卵巢癌患者CYP1A1基因Ile/Val位点多态性,探讨Ile/Val位点多态性与卵巢癌发生的关系.方法:采用等位基因特异性扩增和PCR-RFLP技术,检测CYP1A1基因Ile/Val基因多态性.结果:健康女性Ile、Val等位基因频率为68.9%,31.1%;Ile/Ile,Ile/Val,Val/Val基因型分布频率为44.4%,48.9%,6.7%.卵巢癌患者Ile及Val等位基因的频率分别为63.2%,36.8%;其Ile/Ile,Ile/Val,Val/Val基因型分布频率分别为38.2%,50%,11.8%.结论:张家口地区健康人群和卵巢癌患者CYP1A1基因MspI位点均呈多态性分布,两者基因型分布频率存在显着性差异,说明卵巢癌发病率可能与CYP1A1基因型有关. 相似文献
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1 哈代 (Hardy ,1 90 8年 )和范堡 (Weinberg ,1 90 9年 )先后独立证明了遗传平衡定律 :对于无限大 ,随机交配 ,无突变 ,无自然选择的理想种群而言 ,各基因型的比例可以从一代到另一代维持不变 ,即平衡群体中各基因型的比例与各代相应比例相同。结合该定律 ,可以推导基因频率和基因型频率的公式。1 1 基因频率和基因型频率的概念基因频率 ,指特定基因在一个群体中出现的频率 ;基因型频率 ,指特定基因型个体在一个群体中出现的频率。1 2 基因频率的公式推导假设一个平衡群体中的一对等位基因A、a ,其基因型组合AA、Aa、aa在群体中和… 相似文献
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《中学生物教学》2 0 0 2年第 9期有柴军老师的一篇题为“几种求基因频率的遗传题”的文章 ,其中例 4与例 6涉及伴性遗传 ,原例题及解析过程如下 :例 4 据调查 ,某小学的学生中 ,基因型为XBXB的比例为 42 3 2 % ,XBXb 为 7 3 6% ,XbXb 为 0 3 2 % ,XBY为 46% ,XbY为 4% ,则在该地区XB 和Xb 的基因频率分别为 :A .6% 8% B .8% 92 %C .78% 92 % D .92 % 8%解析 因为女性的性染色体组成为XX ,男性为XY ,而B和b这对等位基因位于X染色体上 ,而Y染色体上无该等位基因 ,因此 ,雌性和雄性不尽相同。XB 的基因频率为… 相似文献
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目的:探讨胰岛素样生长因子1(insulin-like growth factor1,IGF1)基因单核苷酸多态性(SNP)与埃及水牛生长性状的关联性。创新点:发现IGF1基因多态性对埃及水牛的生长性状、血液生化指标和基因表达有显著影响,并为埃及水牛的选育提供重要的分子遗传标记。方法:以200头埃及水牛为试验对象,对其IGF1基因的多态性进行检测,并分析其与生长性状、血液生理生化指标和基因表达的相关性。结论:在水牛IGF1基因多态性分析中,发现两个新的SNP位点(G64A和G280A)分别分布在外显子1和外显子4的非编码区。不同基因型的统计分析表明,G64A和G280A位点的GG基因型水牛个体分别在3~6月龄和6~9月龄的体重(BW)和平均日增重(ADG)均显著高于其他基因型(P0.05)。这两个SNPs位点的组合基因型产生了三种单倍体GG/GG、AG/AG和AA/AA。在3~12月龄的水牛个体中,单倍体基因型与BW和ADG存在显著关联(P0.0001)。纯合的GG/GG单倍体基因型水牛生长性能优于其他水牛。两个SNP位点与半腱肌中IGF1和IGF1R的mRNA水平以及IGF1血清浓度水平相关。此外,GG/GG单倍体水牛表现出较高的mRNA和血清浓度水平。综上所述,这两种SNP位点G64A和G280A可作为埃及水牛生长性状选育的重要分子遗传标记。 相似文献
6.
采用PCR-RFLP方法分析了太湖猪(二花脸)群体中生长激素(growth hormone,GH)、心脏脂肪酸结合蛋白(heart fatty acid-binding protein,H-FABP)和肌细胞生成素(Myogenin,MyoG)基因的多态性.结果表明:在检测的二花脸群体中,GH基因经DraⅠ、MspⅠ酶切无多态性,ApaⅠ酶切出现2个等位基因、3种基因型,基因型频率呈AA>AB>BB的趋势,等位基因A的基因频率大于等位基因B.MyoG基因在PCR1/MspⅠ位点上,基因型AB占据一定的优势;在PCR2/MspⅠ位点上,等位基因A占据绝对的优势,基因型频率趋势为AA>AB>BB.H-FABP基因的HinfⅠ位点上,等位基因h的基因频率有一定的优势.经χ2适合性检验表明,GH、MyoG、H-FABP3个基因在太湖猪(二花脸)群体中的分布均符合Hardy-Weinberg平衡(P>0.05). 相似文献
7.
目的:研究猪PIT-1基因多态性及其对生长性状的遗传效应,旨在寻找改良猪生长性能的分子标记。方法:采用PCR-RFLP方法检测猪PIT-1基因的单核苷酸多态性(SNP),并分析基因多态性与生长性状的关联性。结果:猪PIT-1基因的Rsa I酶切位点存在多态性。其中,杜洛克猪未检测到AA型,BB基因型频率高于AB型,等位基因B频率高于等位基因A,SNP处于低度多态(PIC0.25);淮猪新品系未检测到BB型,AA基因型频率高于AB型,等位基因A频率高于等位基因B,SNP处于低度多态(PIC0.25);杜洛克猪×淮猪新品系未检测到BB型,AB基因型频率高于AA型,等位基因A频率高于等位基因B,SNP处于中度多态(0.25PIC0.5)。关联分析表明,PIT-1基因型对猪平均日增重和饲料转化率的遗传效应都未达到显著水平(P0.05)。结论:杜洛克猪、淮猪新品系、杜洛克猪×淮猪新品系PIT-1基因Rsa I酶位点都存在多态性,但PIT-1基因对猪生长性状没有显著影响。 相似文献
8.
例 1 假设一对等位基因Aa的频率为A =0 .9,a= 0 .1,那么AA、Aa、aa 3种基因型的频率分别是多少 ?解析 首先分析不同基因型的来源。AA、Aa、aa3种基因型都是由含有A、a的雌雄配子随机结合形成的 ,其次分析不同基因型的频率。由于基因型是由组成它的各个基因随机结合 (即一对等位基因中的两个基因同时出现 )而形成的 ,因此 ,基因型频率就等于组成该基因型的各个基因频率的乘积 ,据此列出棋盘表 :雄配子雌配子A(0 .9)a(0 .1)A(0 .9)AA(0 .9× 0 .9)Aa(0 .9× 0 .1)a(0 .1)Aa(0 .9× 0 .1)aa(0 .1× 0 .1) AA =0 .9× 0 .9=0 .81Aa… 相似文献
9.
在自然界中 ,由于存在基因突变、基因重组和自然选择等因素 ,种群的基因频率总是在不断变化的。生物进化的过程实质上就是种群基因频率发生变化的过程。1 基因频率与基因型频率1.1 二者的区别 基因频率指某种基因在某个种群中出现的比例。基因频率 =该基因的总数 /该等位基因的总数 ,实质为某基因在一条染色体上出现的概率。如Aa自交后代中 ,A与a的频率即为基因频率。基因型频率是指群体中某一个体的任何一个基因型所占的百分率。实质为控制某一性状的某两个基因组成在一对同源染色体上出现的频率。如Aa自交后代中AA、Aa、aa的频率。1… 相似文献
10.
依据双亲的基因型,遵循基因分离定律,根据产生的配子类型推算出配子中不同基因的基因频率.
1 双亲基因型为确定类型
双亲的基因型为确定类型.如基因型为AA或aa,则所产生配子的基因频率为A或a等于1;双亲的基因型为Aa,则所产生配子的基因频率为A=1/2,a=1/2. 相似文献
11.
SAT问题(可满足性问题)是计算机科学的核心问题,研究问题的方法很多,利用极小不可满足公式的性质来研究SAT问题是近几年兴起的一个热点研究方向. 文章主要利用,(1,*)-消解和分裂方法研究了差为2的唯一极小不可满足公式集(Unique-MU(2))和差为2的对称极小不可满足公式集(SYM-MU(2))的结构和复杂度. 相似文献
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Sedum alfredii Hance has been identified as zinc (Zn) and cadmium (Cd) co-hyperaccumulator. In this paper the relationships of Zn or Cd hyperaccumulation to the generation and the role of H2O2 in Sedum alfredii H. were examined, The results show that Zn and Cd contents in the shoots of Sedum alfredii H. treated with 1000 μmol/L Zn2 and/or 200 μmol/L Cd2 increased linearly within 15 d. Contents of total S, glutathione (GSH) and H2O2 in shoots also increased within 15 d, and then decreased. Total S and GSH contents in shoots were higher under Cd2 treatment than under Zn2 treatment. However, reverse trends of H2O2 content in shoots were obtained, in which much higher H2O2 content was observed in Zn2 -treated shoots than in Cd2 -treated shoots. Similarly, the microscopic imaging of H2O2 accumulation in leaves using H2O2 probe technique showed that much higher H2O2 accumulation was observed in the Zn2 -treated leaf than in the Cd2 -treated one. These results suggest that there are different responses in the generation of H2O2 upon exposure to Zn2 and Cd2 for the hyperaccumulator Sedum alfredii H. And this is the first report that the generation of H2O2 may play an important role in Zn hyperaccumulation in the leaves. Oar results also imply that GSH may play an important role in the detoxification of dissociated Zn/Cd and the generation of H2O2. 相似文献
14.
Ya-fei Shi Ju-fang Chi Wei-liang Tang Fu-kang Xu Long-bin Liu Zheng Ji Hai-tao Lv Hang-yuan Guo 《Journal of Zhejiang University. Science. B》2013,14(8):696-704
Objective: To test the influence of homocysteine on the production and activation of matrix metalloproteinase-2 (MMP-2) and tissue inhibitors of matrix metalloproteinase-2 (TIMP-2) and on cell migration of cultured rat vascular smooth muscle cells (VSMCs). Also, to explore whether rosuvastatin can alter the abnormal secretion and activation of MMP-2 and TIMP-2 and migration of VSMCs induced by homocysteine. Methods: Rat VSMCs were incubated with different concentrations of homocysteine (50–5 000 μmol/L). Western blotting and gelatin zymography were used to investigate the expressions and activities of MMP-2 and TIMP-2 in VSMCs in culture medium when induced with homocysteine for 24, 48, and 72 h. Transwell chambers were employed to test the migratory ability of VSMCs when incubated with homocysteine for 48 h. Different concentrations of rosuvastatin (10−9–10−5 mol/L) were added when VSMCs were induced with 1 000 μmol/L homocysteine. The expressions and activities of MMP-2 and TIMP-2 were examined after incubating for 24, 48, and 72 h, and the migration of VSMCs was also examined after incubating for 48 h. Results: Homocysteine (50–1 000 μmol/L) increased the production and activation of MMP-2 and expression of TIMP-2 in a dose-dependent manner. However, when incubated with 5 000 μmol/L homocysteine, the expression of MMP-2 was up-regulated, but its activity was down-regulated. Increased homocysteine-induced production and activation of MMP-2 were reduced by rosuvastatin in a dose-dependent manner whereas secretion of TIMP-2 was not significantly altered by rosuvastatin. Homocysteine (50–5 000 μmol/L) stimulated the migration of VSMCs in a dose-dependent manner, but this effect was eliminated by rosuvastatin. Conclusions: Homocysteine (50–1 000 μmol/L) significantly increased the production and activation of MMP-2, the expression of TIMP-2, and the migration of VSMCs in a dose-dependent manner. Additional extracellular rosuvastatin can decrease the excessive expression and activation of MMP-2 and abnormal migration of VSMCs induced by homocysteine. 相似文献
15.
邱碧珍 《福建工程学院学报》2016,(3):289-294
在分析技术接受模型（technology acceptance model,TAM）和任务技术匹配（task technology fit,TTF）整合的必要性的基础上,运用基于TAM和TTF整合的信息技术接受模型,采取二手资料和开放式问卷调查获取影响因素,构建出P2P网络借贷投资者接受模型。模型研究表明:感知风险是阻碍投资者使用P2P平台进行投资的最重要的影响因素。感知有用、感知收益和感知信任从一定程度上能促进投资者使用P2P平台进行投资。政策法规、P2P平台的质量、主观规范、感知行为控制对P2P平台投资也会产生一定的影响。同时,结合本研究模型提出了针对P2P平台应用和推广的建议。 相似文献
16.
The activation of Ca2 entry through store-operated channels by agonists that deplete Ca2 from the endoplasmic reticulum (ER)is a ubiquitous signaling mechanism,the molecular basis of which has remained elusive for the past two decades.Store-operated Ca2 -release-activated Ca2 (CRAC)channels constitute the sole pathway for Ca2 entry following antigen-receptor engagement.In a set of breakthrough studies over the past two years,stromal interaction molecule l(STIM1,tbe ER Ca2 sensor) and Orail(a pore-forming subunit of the CRAC channel)have been identified.Here we review these recent studies and the insights they provide into the mechanism of store-operated Ca2 channels(SOCCs). 相似文献
17.
研究了不同浓度Hg~(2 )对水稻幼苗生长及其过氧化物酶活性的影响．结果表明,经Hg~(2 )胁迫的水稻幼苗,植株矮小,苗鲜重减少;低浓度Hg~(2 )使叶片叶绿素含量增加、POD活性升高．但随着Hg~(2 )浓度增大和处理天数增加,上述指标呈下降趋势,表明植物细胞遭受伤害． 相似文献
18.
Liu YM Zhu SM Wang KR Feng ZY Chen QL 《Journal of Zhejiang University. Science. B》2008,9(11):895-902
Objective: To evaluate the effects oftramadol on the proinflammatory responses in a rat model of incisional pain by investigating its effects on nociceptive thresholds and serum interleukin-6 (IL-6) and IL-2 levels. Methods: Forty-two male Sprague-Dawley (SD) rats scheduled for plantar incision were randomly divided into 7 groups (n=6 in each group). Rats in Group 1 receiving general anesthesia with no incision were served as control; At 30 min before skin incision, Groups 2～5 were given 5 ml normal saline or 1, 10, and 20 mg/kg tramadol, respectively, intraperitoneally (i.p.); Group 6 received 10 mg/kg tramadol after operation; Group 7 received 10 mg/kg tramadol before incision, followed by 200 μg/kg naloxone after operation. Mechanical allodynia was measured by electronic von Frey filament to evaluate the nociceptive thresholds 1 h before incision, and 1 h and 2 h after operation. Serum IL-6 and IL-2 levels were measured by enzyme-linked immunosorbent assay (ELISA) 2 h after operation. Results: Mechanical thresholds decreased significantly and serum IL-6 level increased significantly after operation in Group 2 compared with control (P＜0.01), and these changes were reversed respectively by tramadol in a dose-dependent manner (P＜0.05 and P＜0.01, respectively). IL-2 level remained unchanged after operation in Group 2, but decreased in Group 3 (P＜0.05), then gradually returned to the normal level in Groups 4 and 5. The intraperitoneally injected tramadol (10 and 20 mg/kg) produced a potent and dose-dependent antinocicptive effect on the lesioned paw. The antinocicptive effects of tramadol were partially an-tagonized by naloxone (200 μg/kg), suggesting an additional non-opioid mechanism. Conclusion: The results suggest that tramadol could be a good choice for the treatment of pain under the conditions that immunosuppression may be particularly contraindicated. 相似文献
19.
Chao LI Da-ren LIU Long-yun YE Ling-na HUANG Sanjay JAISWAL Xiao-wen LI Hou-hong WANG Li CHEN 《Journal of Zhejiang University. Science. B》2014,15(6):582-589
Objective
Numerous studies examining the relationship between human epidermal growth factor receptor 2 (HER-2) overexpression and survival in patients with colorectal cancer (CRC) have yielded controversial results. We therefore performed a meta-analysis more precisely to estimate its prognostic value.Methods
Published studies investigating the effect of HER-2 overexpression on CRC survival were identified; the hazard ratios (HRs) and their corresponding 95% confidence intervals (95% CIs) were pooled in terms of disease-specific or overall survival.Results
Eleven studies were included in the meta-analysis. The pooled data showed that HER-2 overexpression was negatively related to CRC survival (HR=1.10, 95% CI: 0.77–1.44). Subgroup analyses regarding test method and study quality also demonstrated little association between HER-2 overexpression and CRC survival (HR=0.89, 95% CI: 0.50–1.29; HR=0.90, 95% CI: 0.43–1.37, respectively).Conclusions
Regardless of several limitations, our study suggested that HER-2 overexpression probably had little impact on CRC survival. 相似文献20.
目的:研究环氧合酶-2(COX-2)对卵巢癌细胞迁移和耐药性的影响及其机制。创新点:本研究发现COX-2对卵巢癌发生发展有一定的促进作用,可以通过上皮间质转化(EMT)途径促进卵巢癌细胞的迁移和顺铂(CDDP)耐药。其抑制剂塞来昔布(CXB)能起到协同抗癌的效果。方法:CCK-8检测CXB和CDDP对SKOV3和ES2细胞的毒性作用。划痕实验评估COX-2对卵巢癌细胞迁移的作用。蛋白质免疫印迹(western blot)和聚合酶链式反应(PCR)检测EMT相关基因和蛋白的表达水平。结论:COX-2可以通过EMT促进卵巢癌细胞迁移和CDDP耐药;CXB可以起到抑制作用,与CDDP协同抗癌。COX-2可以作为卵巢癌治疗的一个潜在靶点。 相似文献