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本文应用扫描电镜和冷冻断裂技术,对16例人体腹膜壁层间皮细胞作了研究,并作了动物实验,结果表明:(1)膈腹膜间皮细胞可区分为立方形细胞和扁平形细胞;(2)在立方形间皮细胞之间有腹膜孔;(3)膈腹膜肌性部和中心腱部均有腹膜孔;(4)在腹膜孔附近,可见由静止型巨噬细胞组成的乳斑;(5)腹膜孔是腹膜腔内物质吸收的主要通道。 相似文献
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用ODO冷冻断裂法观察人膈腹膜间皮细胞和毛细血管的超微结构 总被引：1，自引：0，他引：1
ONO冷冻断裂法是一项新的扫描电镜制样技术。利用这项技术,将样品在-196℃下冷冻,然后把样品掰裂。用扫描电镜观察样品断裂面细胞的内部结构,具有较高的分辨率。并能显示细胞内部细胞器之间的关系。其缺陷是欲观察的断裂面不能预先确定,而是随机的。作者应用ODO冷冻断裂法,对人膈腹膜间皮细胞和毛细血管进行了观察. 相似文献
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借助ODO冷冻断裂扫描电镜制样技术,对人胎膈肌细胞进行研究,获得人体横纹肌细胞、肌原纤维、肌节以及暗带(A带)、明带(I带)和H带的扫描电镜资料。 相似文献
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剖宫产术是解决难产最常施实的手术。随着手术方式的改进 ,以减少手术中的并发症 ,探讨利于产妇的剖宫产术式 ,我院产科自 1 998年 1 2月 1日～1 999年 1 2月 1日 ,1年中分娩总人数 2 71 0人 ,其中剖宫产人数 554人。现将随机选择腹壁横切口腹膜外剖宫产术与腹壁纵切口子宫下段剖宫产术各60例进行比较分析如下 :1 资料与方法两组病例均选择单胎初产妇 ,腹壁横切口腹膜外剖宫产手术组平均年龄 2 6.1岁 ( 2 4～ 32岁 ) ;腹壁纵切口子宫下段剖宫产手术组平均年令 2 5.6岁( 2 4～ 33岁 )。手术指征无明显差异。手术方法依Pfannenstiel s切口 … 相似文献
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间皮细胞微绒毛的超微结构和功能 总被引：1，自引：0，他引：1
以往学者对间皮细胞微绒毛的研究仅限于动物,有些结果也不一致。迄今为止,作者尚未见有关人体腹膜间皮细胞微绒毛超微结构的文献报道。本研究利用透射电镜、扫描电镜和冷冻复型技术对人胚胎(胎龄24～32周)腹膜、胸膜和心包间皮细胞微绒毛的超微结构的观察,为基础和临床医学提供正常的形态学资料。 相似文献
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《西藏科技》2020,(3)
目的对尿毒症临床不同血液净化方式治疗中对机体钙,磷代谢以及心血管病变影响进行研究分析。方法研究采用回顾性分析,将2017年3月份-2018年3月份收治的尿毒症患者采用透析治疗的患者93例,按照透析方式的不同分成两组,腹膜透析组(n=47),血液透析(n=46),对两组患者进行半年的跟踪随访。结果?(1)两组患者的治疗后跟踪随访血钙(2. 19±0. 23) VS(2. 20±0. 17),血磷(1. 79±0. 63) VS(1. 73±0. 96)指标比较无明显差异,P 0. 05,无统计学意义。(2)两组患者接受治疗前各项指标水平比较基本相同,P 0. 05,无统计学意义。经治疗随访半年,两组患者的动脉硬化指数较治疗前有变化,比较有统计学意义(P 0. 05);随访半年两组基线各项指标水平比较无明显差异,P 0. 05,无统计学意义。(3)两组接受治疗前生活质量各项评分比较无差异,经治疗后,生活质量均不同程度改善,腹膜透析组生活质量调查中社会职能,生理评分,情感评分,生命活力均较血液透析组不同程度提高,组间比较差异,P 0. 05,有统计学意义。(4)腹膜透析组不良心血管事件发生率(10. 64%)低于血液透析组(19. 57%),两组比较不良事件发生率有差异,P 0. 05,有统计学意义。结论不同血液净化方式临床应用维持治疗尿毒症患者对钙,磷代谢,动脉硬化指数指数无明显影响;但是腹膜透析组患者生活质量较高于血液透析组,不良心血管事件发生率略低;两种治疗均有优劣势,临床应根据患者的特点选择方式。 相似文献
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异常Q波鉴别的临床价值 总被引：1，自引：0，他引：1
众所周知 ,异常 Q波是心肌梗塞患者典型心电图表现之一 ,但是心电图上所显示的异常 Q波决非均由心肌梗塞所致 ,有很多情况可形成非梗塞性 Q波 ,我院从 1 993年以来发现 1 1 5例 (见下表 )。异常 Q波 115例的分布病种例数 n 男女占 %肺 气 肿 4 5 4 0 5 3 9.1严重心肌缺血 3 0 19112 6.1心 肌 病 15 9613 .0预激症候群 12 5 7 10 .4高 血 钾 85 3 7.0肺 栓 塞 5 4 14 .41 肺栓塞可发生胸骨后闷痛、低血压、呼吸困难 , 、av F导联可出现异常 Q波 ,V1～ 4导联可出现 ST- T改变 ,这些改变常易使人误诊为急性膈面和前间隔心肌梗塞。… 相似文献
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The purpose of this study was to evaluate the efficacy of multi-capillary electrophoresis instrument in clinical laboratory.
An automated clinical capillary electrophoresis system was evaluated for performing serum proteins electrophoresis and immuno-fixation
electrophoresis by subtraction. In this study the performance of capillary electrophoresis was compared with the cellulose
acetate membrane electrophoresis and agarose gel immunofixation electrophoresis for serum proteins. The results of capillary
electrophoresis and cellulose acetate membrane electrophoresis were good (r=0.89∼0.97) for protein fractions and A/G ratio
except for β-gobulin fraction (r=0.60). Both within-run and day to day precisions (CVs) of assay results for 5 main fractions
and A/G ratio (n=10) were between 0.3∼6.3%. The reference ranges of serum protein fractions obtained from 200 healthy individuals
by cellulose acetate membrane electrophoresis were almost equal to that of capillary electrophoresis except for α-1 globulin
fraction. No significant difference of electropherograms between cellulose acetate electrophoresis and capillary electrophoresis
was observed in the abnormal serum such as presence of bilirubin (<20mg/dl), hemoglobin (<300 mg/dl), lipid (Intralipos <1%)
and samples from patients with acute phase response, liver injury, polyclonal hyper gammaglobulinemia or M-proteinemia. The
method of capillary immuno-fixation electrophoresis by subtraction showed good agreement with agarose gel immunofixation electrophoresis
by subtraction identifying 30 monoclonal gammmopathy patient samples. 相似文献
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Herman S D'Souza Geraldine Menezes T Venkatesh 《Indian journal of clinical biochemistry : IJCB》2002,17(1):1-4
Our study aimed at comparing lead and zinc protoporphyrin (ZPP) levels in capillary and venous blood samples in a small population
and to employ an easier method of sample collection for a major screening program in school children in major Indian cities.
An awareness program on lead and its effects was conducted in two different schools. A total of thirty urban school children
from South India, with an age group between 4–12 years consented for dual blood sampling and reported for the study. Venous
and capillary blood samples were obtained simultaneously. Blood lead and zinc protoporphyrin (ZPP) levels were estimated using
ESA Lead Analyzer and Haematofluorometer respectively. A significant correlation between capillary and venous ZPP (r=0.98)
and lead (r=0.99) was observed. Rank sum test showed that there is no statistically significant difference between capillary
and venous ZPP (P=0.891) and lead (P=0.672) values. This pilot study recommends that screening for lead may be done using
capillary blood samples since significant correlation is observed between capillary and venous blood measurements. Obtaining
samples using this mode is a non-invasive, less expensive, quick and easy method in children. Appropriately performed capillary
sampling may be considered as an acceptable alternative to venipuncture for screening of blood for lead poisoning both in
children and adults. 相似文献
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A simple and effective universal serial bus (USB) flash disk type microfluidic chip electrophoresis (MCE) was developed by using poly(dimethylsiloxane) based soft lithography and dry film based printed circuit board etching techniques in this paper. The MCE had a microchannel diameter of 375 μm and an effective length of 25 mm. Equipped with a conventional online electrochemical detector, the device enabled effectively separation of bovine serum albumin, lysozyme, and cytochrome c in 80 s under the ultra low voltage from a computer USB interface. Compared with traditional capillary electrophoresis, the USB flash disk type MCE is not only portable and inexpensive but also fast with high separation efficiency. 相似文献
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The solid phase polymerase chain reaction (PCR) on a gel-based microarray system was studied under various durations of individual stages of the PCR cycle and spatial restriction of the reaction volume. Combining the experimental study with numerical modeling, we demonstrated that the diffusion of the PCR product in and out of a microarray element during the annealing and melting stages, respectively, is the main factor responsible for distinctive features of the studied type of PCR. The restriction of reaction volume leads to faster PCR signal growth. Particularly, the capillary array, whereby gel-based microarray elements are located on a glass bar inserted into capillary chamber, was found to be a suitable format for the development of the platform. 相似文献
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Recent studies have demonstrated the presence of prostate specific antigen (PSA) in cord blood of male as well as female babies. The placental progesterone and estradiol up-regulate the synthesis and secretion of PSA in Placenta. This PSA is presumed to play a role in intrauterine growth of fetus by virtue of its proteolytic action on several substrates including insulin-like-growth-factor-binding-protein-3, insulin chains and Interleukin-2. This study was planned with the objective of correlating the levels of PSA in cord blood to gestation at delivery, the type of delivery and gender of the fetus. Fifty-seven cord blood samples were collected from the umbilical cord during delivery or mid-trimester abortion and analyzed for PSA using ‘Active PSA DSL-9700 ultra sensitive’ kit employing two-site immuno-radiometric assay principle and having a detection limit of 0.001 ng/ml. Mean PSA levels in cord blood were found to be 0.112 ± 0.027 ng/ml. The concentration of PSA in cord blood was found to be higher in case of higher gestational age, male baby and operative delivery. 50 % of cord bloods for female babies had PSA below detection limit (range <0.001–0.460 ng/ml), while all the male samples had detectable PSA (range 0.11–0.973 ng/ml). Higher Progesterone levels found in prenatal maternal blood in case of male babies may be responsible for the higher cord blood PSA. Mean cord blood PSA was 0.150 ± 0.150 ng/ml in forceps delivery and 0.078 ± 0.012 ng/ml in normal vaginal delivery. Forceps delivery causes much more stress and strain as compared to a normal vaginal delivery, resulting in increased levels of adrenal glucocorticoids, and therefore, higher cord blood PSA. 相似文献
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Jeonghun Nam Justin Kok Soon Tan Bee Luan Khoo Bumseok Namgung Hwa Liang Leo Chwee Teck Lim Sangho Kim 《Biomicrofluidics》2015,9(6)
A novel microfluidic device which consists of two stages for particle focusing and separation using a viscoelastic fluid has been developed. A circular capillary tube was used for three-dimensional particle pre-alignment before the separation process, which was inserted in a polydimethylsiloxane microchannel. Particles with diameters of 5 and 10 μm were focused at the centerline in the capillary tube, and the location of particles was initialized at the first bifurcation. Then, 5 and 10 μm particles were successfully separated in the expansion region based on size-dependent lateral migration, with ∼99% separation efficiency. The proposed device was further applied to separation of MCF-7 cells from leukocytes. Based on the cell size distribution, an approximate size cutoff for separation was determined to be 16 μm. At 200 μl/min, 94% of MCF-7 cells were separated with the purity of ∼97%. According to the trypan blue exclusion assay, high viability (∼90%) could be achieved for the separated MCF-7 cells. The use of a commercially available capillary tube enables the device to be highly versatile in dealing with particles in a wide size range by using capillary tubes with different inner diameters. 相似文献
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A transverse diffusion mediated capillary microanalysis method has been developed for screening of neuraminidase inhibitors from traditional Chinese medicine. The enzyme, substrate and inhibitors were sequentially injected, mixed efficiently by transverse diffusion of laminar flow profiles, then incubated and separated in the same capillary. To enhance the mixing efficiency of reactants, running buffer was injected by alternately applying +5 kPa and −5 kPa at the capillary inlet and the procedure was repeated three times. The capillary electrophoresis (CE) separation conditions and reactants mixing conditions were optimized. Dual-wavelength detection was employed to eliminate the interference with natural compounds. The method has been applied to determine the kinetics constant of neuraminidase and screen 12 compounds from traditional Chinese medicine. Four compounds have been found to be positive for enzyme inhibition. The results are in good agreement with those reported in the literature. The method realized the mixing of substrate and enzyme with identical electrophoretic mobility. This novel CE method was simple, rapid, economic, and fully automated. Therefore, it was appropriate for neuraminidase inhibitors screening and could be extended to other high-throughput screening of active components from traditional Chinese medicine. 相似文献
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Amit Kumar Mani Tiwari Abbas Ali Mahdi Sanjay Mishra 《Indian journal of clinical biochemistry : IJCB》2018,33(1):31-37
Iron deficiency anemia is one of the causes that lead to significant mortality and morbidity among pregnant women and fetus. The present study was undertaken to explore oral iron supplementation can modify the metal contents in pregnant anemic women. Iron and folic acid supplementations was given to 500 anemic women (mild = 200, moderate = 200, and severe = 100) and 100 age matched non-anemic controls daily for 100 days. Blood index values and plasma trace minerals were estimated as per standard protocols. Haemoglobin and ferritin levels were found significantly increased (p < 0.001) in anemic and control subjects after treatment. Moreover, the serum transferring receptor levels and total iron binding capacity were found significantly decreased in all treated groups. Iron (Fe), zinc (Zn) and copper (Cu) levels were found increased (p < 0.01) after oral iron supplementation groups. Moreover, selenium (Se) manganese (Mn) and were found to be decreased in all treated groups. Data provides the conclusion that iron and folic acid supplementation recovered the essential trace minerals, except manganese, which may lead to various complications including peroxidation of vital body molecules resulting in increased risk for pregnant women as well as fetus. 相似文献
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Jasna Lenicek Krleza 《Biochemia medica : ?asopis Hrvatskoga dru?tva medicinskih biokemi?ara / HDMB》2014,24(3):350-358
Introduction:
Capillary sampling is increasingly used to obtain blood for laboratory tests in volumes as small as necessary and as non-invasively as possible. Whether capillary blood sampling is also frequent in Croatia, and whether it is performed according to international laboratory standards is unclear.Materials and methods:
All medical laboratories that participate in the Croatian National External Quality Assessment Program (N = 204) were surveyed on-line to collect information about the laboratory’s parent institution, patient population, types and frequencies of laboratory tests based on capillary blood samples, choice of reference intervals, and policies and procedures specifically related to capillary sampling. Sampling practices were compared with guidelines from the Clinical and Laboratory Standards Institute (CLSI) and the World Health Organization (WHO).Results:
Of the 204 laboratories surveyed, 174 (85%) responded with complete questionnaires. Among the 174 respondents, 155 (89%) reported that they routinely perform capillary sampling, which is carried out by laboratory staff in 118 laboratories (76%). Nearly half of respondent laboratories (48%) do not have a written protocol including order of draw for multiple sampling. A single puncture site is used to provide capillary blood for up to two samples at 43% of laboratories that occasionally or regularly perform such sampling. Most respondents (88%) never perform arterialisation prior to capillary blood sampling.Conclusions:
Capillary blood sampling is highly prevalent in Croatia across different types of clinical facilities and patient populations. Capillary sampling procedures are not standardised in the country, and the rate of laboratory compliance with CLSI and WHO guidelines is low. 相似文献20.
戴云山黄山松林土壤水分物理性质空间变异特征与格局 总被引：2，自引：1，他引：1
土壤水分是植被格局形成和演变的关键因子,其空间异质性研究有助于理解高海拔植物幼苗更新对环境的响应机制。为此,采用地统计与GIS空间分析技术,对戴云山自然保护区陈板岭头1hm2黄山松林样地上100个10m×10m土壤水分实测数据的空间变异特征与格局进行分析。结果表明:土壤含水量、土壤容重、毛管孔隙度和非毛管孔隙度变异系数在12.26%~43.79%之间,均为中等变异;最佳半方差函数理论模型均为高斯模型;由随机性因素引起的空间变异占总变异的比例依次为0.15%、0.03%、0.18%和0;有效变程分别为19.40m、17.32m、17.32m和14.90m。4个指标各向异性明显,空间自相关范围总体均随空间距离增大而呈减弱趋势。分形维数:毛管孔隙度(1.793)土壤含水量(1.669)土壤容重(1.406)非毛管孔隙度(1.348),与半方差函数曲线所反映的规律相一致。从空间分布特征看,各指标均表现为带状和斑块状镶嵌分布。土壤含水量在东南方向、西边中部和北向中部都有高值区,而西南-东北向较低。毛管孔隙度与土壤含水量空间分布相似,土壤容重和非毛管孔隙的空间分布与土壤含水量呈互补趋势。土壤水分-物理性质各指标的空间变异和格局与研究区地形、植被覆盖以及微生境干扰有关。土壤含水量对黄山松幼苗更新有制约作用。研究结果可为黄山松林土壤水分取样设计和更新苗格局-过程机理研究提供理论依据。 相似文献