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1.
AtCRE1 is known to be a cytokinin receptor in Arabidopsis. The AtCRE1 protein contains CHASE domain at the N-terminal part, followed by a transmitter (histidine kinase) domain and two receiver domains. The N-terminal CHASE domain of AtCRE1 contains putative recognition sites for cytokinin. Five CHASE domains containing proteins were found in rice, OsCRL1a, OsCRL1b, OsCRL2, OsCRL3, and OsCRL4. OsCRL1a, OsCRL1b, OsCRL2 and OsCRL3 contain the four domains existing in CRE1, whereas OsCRL4 only contains the CHASE domain and a putative Ser/Thr protein kinase domain. The authors cloned the encoding gene OsCRL4 and found that it represents a new member of the cytokinin receptor protein in rice. 相似文献
2.
AtCRE1 is known to be a cytokinin receptor inArabidopsis. TheAtCRE1 protein contains CHASE domain at the N-terminal part, followed by a transmitter (histidine kinase) domain and two receiver
domains. The N-terminal CHASE domain ofAtCRE1 contains putative recognition sites for cytokinin. Five CHASE domains containing proteins were found in rice,osCRL1a,OsCRL1b,OsCRL2,OsCRL3, andOsCRL4.OsCRL1a,OsCRL1b,OsCRL2 andOsCRL3 contain the four domains existing inCRE1, whereasOsCRL4 only contains the CHASE domain and a putative Ser/Thr protein kinase domain. The authors cloned the encoding geneOsCRL4 and found that it represents a new member of the cytokinin receptor protein in rice.
Project supported by the National Natural Science Foundation of China 相似文献
3.
Form ins蛋白是一类结构蛋白,它的生物学功能与肌动蛋白密切相关。其基本结构除了FH 1和FH 2结构域以外,动物和真菌中还具有FH 3、双螺旋结构域等等,而在植物中则没有。根据蛋白N端有无信号肽和跨膜结构域,植物中的Form ins蛋白被典型地分为I、II二类。Form ins蛋白分布广泛,在拟南芥和水稻中分别预测出有21个和16个Form ins蛋白的存在。Form ins蛋白的生物学功能主要表现在参与了肌动蛋白的组装以及作为信号传递链的组成部分。 相似文献
4.
李艳花 《雁北师范学院学报》2011,27(3)
应用RT-PCR的方法从拟南芥cDNA中克隆了AFH14的全序列,并对其功能结构域进行分析。发现所克隆的目的基因编码区全长3 102 bp,与基因组序列比对发现该基因包含18个内含子和18个外显子,编码1 033个氨基酸残基。通过与其它formin成员的序列比对分析,发现AFH14是一个典型的拟南芥II型formin,包括PTEN结构域。 相似文献
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Sequence analysis and expression pattern of MGTA1 gene in rice blast pathogen Magnaporthe grisea 总被引：1，自引：0，他引：1
INTRODUCTION Fungi cause most plant diseases of all groups of microbes. During their infection cycles, fungal pathogens must undergo two key processes: first, penetration through cuticles into host plant cells; second, colonization in host cells utilizing nutrients from their hosts. To penetrate host cells, fungi de-velop a series of specialized infection structures such as appressorium, penetration peg, and infection hypha. The appressorium-mediated penetration is a process typical of s… 相似文献
6.
Sérgio Gaitas Margarida Alves Martins 《International Journal of Inclusive Education》2017,21(5):544-556
This study analyses teacher perceived difficulty in implementing differentiated instructional strategies in regular classes. The participants were 273 Portuguese primary school teachers with teaching experience ranging from 1 to 33 years. A 39-item questionnaire was used to evaluate teacher perceived difficulty in relation to different instructional strategies. Teacher responses were factor analysed and five different domains were identified: (1) activities and materials; (2) assessment; (3) management; (4) planning and preparation; and (5) classroom environment. Results showed that with the exception of the classroom environment domain, all the instruction practices in the remaining domains were considered to be difficult. In particular, the most difficult practices were under the domain of activities and materials and were associated with the adaptation of curricular elements (content, process, and product) based on student characteristics (readiness, interest, and learning profiles). The results also revealed a strong association between the activities and materials domain and the assessment domain. Some implications of these findings for initial teacher training and teacher professional development programmes are discussed. 相似文献
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Although studies have reported a linkage between young pregnancy and child maltreatment risk, it is still unclear about what factors place young mothers at greater risk of maltreating their child in Chinese context. Based on the socio-ecological model, risk factors in 4 domains: family background/structure, maternal stressors, mother-child interaction, and child behavioral issue in relation to physical assault, neglect, both physical assault and neglect, and either physical assault or neglect among Chinese young mothers in Hong Kong were assessed. 392 young mothers were recruited from an integrated supportive program for young mothers. The mean age of mothers at delivery was 21.8 (SD = 3.0) and 52.3% were married. Individual risk factors and cumulative risk domains related to different child maltreatment groups were examined. Our results show both overlapping and unique risk factors across the domains associated with physical assault and neglect. Further, young families exposed to higher number of risk domains show higher rates for physical assault and neglect, co-occurrence of physical assault and neglect, and either form of maltreatment. In addition, various risk domains were found to be particularly important for different forms of maltreatment: family background/structure domain was found to be an important risk domain for neglect; mother-child interaction domain for both physical assault and neglect; family background/structure and maternal stressors domains for either physical assault or neglect. Closer examination of a subgroup of adolescent mothers aged 18 and below shows that family background/structure was an important risk domain for this group. 相似文献
9.
Xin-chun Zhang Xin Yu Hui-juan Zhang Feng-ming Song 《Journal of Zhejiang University. Science. B》2009,10(10):731-739
We cloned and characterized a rice gene OsBIABPI encoding an AMP-binding protein. The full-length cDNA of OsBIABP1 is 1912-bp long and is predicted to encode a 558-aa protein. OsBIABP1 contains a typical AMP-binding signature motif and shows high similarity to members of AMP-binding protein family. OsBIABP1 is expressed in stems, leaves and flowers of rice plants, but is not expressed, or expressed at a very low level, in rice roots. The expression of OsBIABP1 was induced by some defense-related signal molecules, e.g., salicylic acid (SA), benzothiadiazole, jasmonic acid (JA), and 1-amino cyclopropane-1-carboxylic acid, which mediate SA-and JA/ethylene (ET)-dependent defense signaling pathways, respectively. Furthermore, the expression of OsBIABP1 is activated by the infection of Magnaporthe oryzae, and the induced expression is quicker and stronger during early stages of pathogenesis in incompatible interaction than that in compatible interaction between rice and M. oryzae. Our results suggest that OsBIABP1 may be a defense-related AMP-binding protein that is involved in the regulation of defense re-sponse through SA and/or JA/ET signaling pathways. 相似文献
10.
C A Brownell 《Child development》1988,59(3):675-685
Toddlers' combinatorial abilities were examined over several behavioral domains as a function of age (20 and 27 months) and task demands (familiarity of components, length of combinations, complexity of combinations, centered/decentered focus of action, behavioral domain). 6 specific domains were represented. Children's combinations were observed during elicited imitation in 4 of the domains (object play, pretense, social play, motor play) and spontaneous production in 2 others (language and peer-directed social overtures). Relative to younger children, older children produced more combinations of at least 2 or 3 discrete behaviors in every domain, including peer interaction, and fewer noncombinatorial behaviors. Consistent relations were also found across domains for production of combinations, that is, children who produced more combinations in one domain also did so in others. Finally, 4 of the 5 manipulated task demands proved to affect performance. Results are discussed in terms of possible age-related constraints on combinatorial skills that operate at a general, cross-domain level during toddlerhood. 相似文献
11.
黄婕 《连云港职业技术学院学报》2007,20(1):16-19
数字水印将感觉不到的信号嵌入到声频、视频和图像中以实现不同的目的.从有关数字水印的基本原理及其主体框架出发.将数字水印按不同角度划分为不同种类,并从空间域算法、变换域算法、压缩域算法、NEC算法及生理模型算法的角度讨论了数字水印技术,指出未来值得关注的研究领域一是数字水印的基本理论和算法的研究;二是数字水印的标准化研究;三是数字水印的网络应用研究;四是与其他领域先进技术的结合研究. 相似文献
12.
In separate studies on academic self-concept, previous research has shown: (1) the distinctiveness of a cognitive and an affective component, (2) the domain specificity of self-concepts, (3) the reciprocal effects of self-concept and achievement, (4) the internal/external frame of reference in self-concept development, (5) the reciprocal effects of the internal/external frame of reference, (6) the big-fish-little-pond effect, and (7) the interrelatedness of self-concepts in similar domains. The present study demonstrates that all of these seven findings are replicable and may be synthesized in a single study with a sample of students in Singapore. Secondary 1 students (7th graders; N = 275) were surveyed with 24 items about their academic self-concepts in physics, English, and math in two components (cognitive and affective), and their respective achievement scores were recorded over two time points. Confirmatory factor analysis found that the cognitive and affective components of academic self-concept were separable. The students’ self-concepts in different curriculum domains were distinct, supporting the domain specificity of self-concepts. The frame of reference and reciprocal effects were both supported, but only for the cognitive component of self-concept. Positive and statistically significant correlations between physics and math suggest that these curriculum domains were interrelated. Results of self-concept studies in schools can encourage and guide the design of interventions that could enhance students’ self-concept for positive sustainable effects on desirable educational outcomes. Attempts to improve learning outcomes should emphasize an enhancement of specific components of academic self-concept in domain-specific and related curriculum domains for optimal effects. 相似文献
13.
Expanding research on the relative impact of different autonomy-supportive strategies employed by teachers across domains, the present study investigated the variation in 4 lesson-specific autonomy-supportive strategies (providing choices, rationales, accepting frustration, and stimulating interests) and 6 aspects of students' motivation and engagement in 2 domains with a repeated measurement design. For 3 weeks, 202 Dutch students from 8 eighth grade classes and 1 ninth-grade class and 12 teachers completed lesson-specific measures at the end of Math and German lessons. Students' perceptions of teachers' autonomy support and their motivation and engagement varied considerably across lessons within a domain (variance at the within-student level ranged from 19% to 51%). In random intercept-random slope models, we found that all autonomy-supportive strategies showed meaningful associations with aspects of students’ motivation and engagement. We did not find substantial domain-dependency in the associations between autonomy support and the outcomes. 相似文献
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Xuan Zhou Jia-qi Li Li-jie Wei Meng-zhou He Jing Jia Jing-yi Zhang Shao-shuai Wang Ling Feng 《Journal of Zhejiang University. Science. B》2020,21(12):990
Disulfide-bond A oxidoreductase-like protein (DsbA-L) is a molecular chaperone involved in the multimerization of adiponectin. Recent studies have found that DsbA-L is related to metabolic diseases including gestational diabetes mellitus (GDM), and can be regulated by peroxisome proliferator-activated receptor γ (PPARγ) agonists; the specific mechanism, however, is uncertain. Furthermore, the relationship between DsbA-L and the novel adipokine chemerin is also unclear. This article aims to investigate the role of DsbA-L in the improvement of insulin resistance by PPARγ agonists in trophoblast cells cultured by the high-glucose simulation of GDM placenta. Immunohistochemistry and western blot were used to detect differences between GDM patients and normal pregnant women in DsbA-L expression in the adipose tissue. The western blot technique was performed to verify the relationship between PPARγ agonists and DsbA-L, and to explore changes in key molecules of the insulin signaling pathway, as well as the effect of chemerin on DsbA-L. Results showed that DsbA-L was significantly downregulated in the adipose tissue of GDM patients. Both PPARγ agonists and chemerin could upregulate the level of DsbA-L. Silencing DsbA-L affected the function of rosiglitazone to promote the phosphatidylinositol 3-kinase (PI3K)-protein kinase B (PKB)/AKT pathway. Therefore, it is plausible to speculate that DsbA-L is essential in the environment of PPARγ agonists for raising insulin sensitivity. Overall, we further clarified the mechanism by which PPARγ agonists improve insulin resistance. 相似文献
16.
《Journal of research on educational effectiveness》2013,6(4):552-576
Abstract: In observational studies, selection bias will be completely removed only if the selection mechanism is ignorable, namely, all confounders of treatment selection and potential outcomes are reliably measured. Ideally, well-grounded substantive theories about the selection process and outcome-generating model are used to generate the sample of covariates. However, covariate selection is more heuristic in actual practice. Using two empirical data sets in a simulation study, we investigate four research questions about bias reduction when the selection mechanism is not known but many covariates are measured: (1) How important is the conceptual heterogeneity of the covariate domains in the data set? (2) How important is the number of covariates assessing each domain? (3) What are the joint effects of this conceptual heterogeneity and of the number of covariates per domain? (4) What happens to bias reduction when the set of covariates is deliberately impoverished by removing the covariates most responsible for selection bias, thus ensuring a slightly smaller but still heterogeneous set of covariates? The results indicate: (1) increasingly more bias is reduced as the number of covariate domains and the number of covariates per domain increase, though the rate of bias reduction is diminishing in each case; (2) sampling covariates from multiple heterogeneous covariate domains is more important than choosing many measures from fewer domains; (3) the most heterogeneous set of covariate domains removes almost all of the selection bias when at least five covariates are assessed in each domain; and (4) omitting the most crucial covariates generally replicates the pattern of results due to the number of domains and the number of covariates per domain, but the amount of bias reduction is less than when all variables are included and will surely not satisfy all consumers of causal research. 相似文献
17.
董正华 《商丘师范学院学报》2001,17(4):37-41
给出有界星形圆型域上一类螺旋映照的增长定理，推广了已知的关于星形映照的结果，所讨论的域是非常广泛的，包括了复椭球和四类典型域，所讨论的映照类也是非常广泛的。 相似文献
18.
Qi-chao ZHAO Ming-hong LIU Xian-wen ZHANG Chao-yang LIN Qing ZHANG Zhi-cheng SHEN 《Journal of Zhejiang University. Science. B》2015,16(10):824-831
Insect resistance and glyphosate tolerance have been two of the most important traits in the genetic improvement of various crops. In this study, two Bacillus thuringiensis (Bt) insecticidal genes, Cry1Ac and Cry1Ig, and a modified glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene (G10) were combined into a single transferred DNA (T-DNA) fragment and introduced into rice by Agrobacterium-mediated transformation. A transgenic line with single-copy T-DNA insertion named GAI-14 was found to be highly resistant to striped stem borer and rice leaf roller, and tolerant to glyphosate. Analysis of T-DNA border sequence suggested that the transgenes were inserted at the chromosome 3 and appeared to have not interrupted any known or putative genes. A field trial observed no significant difference in the basic agronomic traits between GAI-14 and the recipient rice. 相似文献
19.
Embryogenic calli were induced from the seeds of creeping bentgrass (Agrostis palustris Huds.) cv. Regent and colonial bentgrass (Agrostis Tenuis Sibth. Fl. Oxen.) cv. Tiger. The embryogenic calli were precultured on fresh medium for 4-7 days and then co-cultivated with Agrobacterium tumefaciens, LBA4404, which contains plasmid vector-pSBGM harboring bar coding region, synthetic green fluorescent protein (sGFP) coding region and matrix attachment region (MAR). After 3 days of co-cultivation, the calli were washed thoroughly and transferred to MS medium containing 2 mg/L of 2, 4-D, 12-15 mg/L phosphinothricin (PPT) and 250 mg/L of cefotaxime. After 2-3 months of selection, the actively growing calli of 'Regent' and 'Tiger' were transferred to MS medium with 12-15 mg/L PPT and 250 mg/L cefotaxime for regeneration. The putative transformants were maintained on MS medium with 3 mg/L PPT for long period but control died within 1 month. After establishing in greenhouse, the transformants also showed strong resistance to 0.4% of herbicide Basta but control plants died within 2 weeks. Under confocal microscope, both young leaves and roots showed significant GFP expression. PCR analysis revealed the presence of a DNA fragment of GFP gene at the expected size (380 bp) in the transformants and its absence in a randomly selected control plant. 相似文献