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Mothers and babies have a physiologic need to be together at the moment of birth and during the hours and days that follow. Keeping mothers and babies together is a safe and healthy birth practice. Evidence supports immediate, uninterrupted skin-to-skin care after vaginal birth and during and after cesarean surgery for all stable mothers and babies, regardless of feeding preference. Unlimited opportunities for skin-to-skin care and breastfeeding promote optimal maternal and child outcomes. This article is an updated evidence-based review of the “Lamaze International Care Practices That Promote Normal Birth, Care Practice #6: No Separation of Mother and Baby, With Unlimited Opportunities for Breastfeeding,” published in The Journal of Perinatal Education, 16(3), 2007.  相似文献   

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Large-scale parallel gene expression analysis has provided a greater ease for investigating the underlying mechanisms of Duchenne muscular dystrophy (DMD). Previous studies typically implemented variance/regression analysis, which would be fundamentally flawed when unaccounted sources of variability in the arrays existed. Here we aim to identify genes that contribute to the pathology of DMD using partial least squares (PLS) based analysis. We carried out PLS-based analysis with two datasets downloaded from the Gene Expression Omnibus (GEO) database to identify genes contributing to the pathology of DMD. Except for the genes related to inflammation, muscle regeneration and extracellular matrix (ECM) modeling, we found some genes with high fold change, which have not been identified by previous studies, such as SRPX, GPNMB, SAT1, and LYZ. In addition, downregulation of the fatty acid metabolism pathway was found, which may be related to the progressive muscle wasting process. Our results provide a better understanding for the downstream mechanisms of DMD.  相似文献   

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With the availability of the whole genome sequence of Escherichia coli or Corynebacterium glutamicum, strategies for directed DNA manipulation have developed rapidly. DNA manipulation plays an important role in understanding the function of genes and in constructing novel engineering bacteria according to requirement. DNA manipulation involves modifying the autologous genes and expressing the heterogenous genes. Two alternative approaches, using electroporation linear DNA or recombinant suicide plasmid, allow a wide variety of DNA manipulation. However, the over-expression of the desired gene is generally executed via plasmid-mediation. The current review summarizes the common strategies used for genetically modifying E. coli and C. glutamicum genomes, and discusses the technical problem of multi-layered DNA manipulation. Strategies for gene over-expression via integrating into genome are proposed. This review is intended to be an accessible introduction to DNA manipulation within the bacterial genome for novices and a source of the latest experimental information for experienced investigators.  相似文献   

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The aim of this study was to describe women''s experience of pregnancy. The setting was the Alternative Birth Center at Sahlgrenska University Hospital in Göteborg, Sweden, from 1996 to 1997. Anonymous diaries were written by 12 women and analyzed using a hermeneutical/phenomenological approach. The essential structure and interpretation of women''s experience of pregnancy can be expressed as “transition to the unknown,” which includes three themes: (1) meeting one''s life situation, (2) meeting something inevitable, and (3) preparing for the unknown.The essential structure and interpretation of women''s experience of pregnancy can be expressed as “transition to the unknown” …  相似文献   

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Objective: To investigate the protective effects and mechanisms of action of dexamethasone and Salvia miltiorrhiza on multiple organs in rats with severe acute pancreatitis (SAP). Methods: The rats were divided into sham-operated, model control, dexamethasone treated, and Salvia miltiorrhiza treated groups. At 3, 6, and 12 h after operation, the mortality rate of different groups, pathological changes, Bcl-2-associated X protein (Bax) and nuclear factor-κB (NF-κB) protein expression levels in multiple organs (the pancreas, liver, kidneys, and lungs), toll-like receptor 4 (TLR-4) protein levels (only in the liver), intercellular adhesion molecule 1 (ICAM-1) protein levels (only in the lung), and terminal deoxynucleotidy transferase mediated deoxyuridine triphosphate (dUTP) nick end labeling (TUNEL) staining expression levels, as well as the serum contents of amylase, glutamate-pyruvate transaminase (GPT), glutamic-oxaloacetic transaminase (GOT), blood urea nitrogen (BUN), and creatinine (CREA) were observed. Results: The mortality rate of the dexamethasone treated group was significantly lower than that of the model control group (P<0.05). The pathological changes in multiple organs in the two treated groups were relieved to different degrees (P<0.05 and P<0.01, respectively), the expression levels of Bax and NF-κB proteins, and apoptotic indexes of multiple organs were reduced (P<0.05 and P<0.01, respectively). The contents of amylase, GPT, GOT, BUN, and CREA in the two treated groups were significantly lower than those in model control groups (P<0.05 and P<0.01, respectively). The expression level of ICAM-1 protein in the lungs (at 3 and 12 h) in the dexamethasone treated group was significantly lower than that in the Salvia miltiorrhiza treated group (P<0.05). The serum contents of CREA (at 12 h) and BUN (at 6 h) of the Salvia miltiorrhiza treated group were significantly lower than those in the dexamethasone treated group (P<0.05). Conclusions: Both dexamethasone and Salvia miltiorrhiza can reduce the inflammatory reaction, regulate apoptosis, and thus protect multiple organs of rats with SAP.  相似文献   

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目的:分析CYP81A6基因在苯达松及甲磺隆处理下的诱导表达模式,解释该基因与两种除草剂代谢相关的可能原因。创新点:从两种除草剂降解途径中产生的小分子物质的结构相似性出发,通过基因诱导表达的特点分析,解释CYP81A6和两种除草剂降解相关的原因。方法:通过实时定量聚合酶链反应(PCR)来分析基因表达的特点;利用CYP81A6启动子与GUS报告基因构建的载体来分析组织特异性表达;通过亚细胞定位来确定CYP81A6发挥功能的场所。结论:CYP81A6基因受苯达松及甲磺隆诱导,在不同的时间点开始上调,说明了甲磺隆的降解中间产物可以诱导这个基因的表达;CYP81A6是组成型表达,在根、茎、叶中均有表达;亚细胞定位结果证明CYP81A6是一个内质网上的蛋白。  相似文献   

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In 2013, Childbirth Connection published findings from a U.S. study of women’s pregnancy, childbirth, and postpartum experiences, Listening to Mothers III. In this issue of The Journal of Perinatal Education, we publish the major survey findings of both the pregnancy and birth survey and the postpartum survey. This editorial discusses some of the major findings of the childbirth survey. Listening to what mothers have to say about their experiences suggests a mandate to “listen up” to what mothers are telling us and continue to advocate for evidence-based maternity care. Articles in this issue of the journal are presented.  相似文献   

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We have developed experiments and materials to model human genetics using rapid cycling Brassica rapa, also known as Fast Plants. Because of their self-incompatibility for pollination and the genetic diversity within strains, B. rapa can serve as a relevant model for human genetics in teaching laboratory experiments. The experiment presented here is a paternity exclusion project in which a child is born with a known mother but two possible alleged fathers. Students use DNA markers (microsatellites) to perform paternity exclusion on these subjects. Realistic DNA marker analysis can be challenging to implement within the limitations of an instructional lab, but we have optimized the experimental methods to work in a teaching lab environment and to maximize the “hands-on” experience for the students. The genetic individuality of each B. rapa plant, revealed by analysis of polymorphic microsatellite markers, means that each time students perform this project, they obtain unique results that foster independent thinking in the process of data interpretation.  相似文献   

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Penicillium digitatum is the most important pathogen of postharvest citrus. Gene targeting can be done in P. digitatum using homologous recombination via Agrobacterium tumefaciens mediated transformation (ATMT), but the frequencies are often very low. In the present study, we replaced the Ku80 homolog (a gene of the non-homologous end-joining (NHEJ) pathway) with the hygromycin resistance cassette (hph) by ATMT. No significant change in vegetative growth, conidiation, or pathogenicity was observed in Ku80-deficient strain (ΔPdKu80) of P. digitatum. However, using ΔPdKu80 as a targeting strain, the gene-targeting frequencies for both genes PdbrlA and PdmpkA were significantly increased. These results suggest that Ku80 plays an important role in homologous integration and the created ΔPdKu80 strain would be a good candidate for rapid gene function analysis in P. digitatum.  相似文献   

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This feature is designed to point CBE—Life Sciences Education readers to current articles of interest in life sciences education as well as more general and noteworthy publications in education research.This feature is designed to point CBE—Life Sciences Education readers to current articles of interest in life sciences education, as well as more general and noteworthy publications in education research. URLs are provided for the abstracts or full text of articles. For articles listed as “Abstract available,” full text may be accessible at the indicated URL for readers whose institutions subscribe to the corresponding journal. This themed issue focuses on recent studies of concepts and conceptualization—from how textbook images and students’ attitudes and levels of acceptance can influence their understandings to design of tools that educators can use to understand what their students know.1. Novick LR, Stull AT, Catley KM (2012). Reading phylogenetic trees: the effects of tree orientation and text processing on comprehension. BioScience 62, 757–764.[Abstract available: www.jstor.org/stable/10.1525/bio.2012.62.8.8]Cladograms—branching, nested hierarchical diagrams drawn in a variety of formats—are commonly used to depict how organisms might be related. Although differently formatted cladograms can convey the same information, informationally equivalent cladograms are not necessarily equivalent “computationally,” that is, with respect to the ease with which observers interpret and use them. Because diagonal cladograms with a slanting up-to-the-right (UR) orientation are most commonly used in college-level textbooks, the authors explored whether a diagonal cladogram drawn with a UR backbone line is computationally equivalent to its informationally equivalent mirror-image, drawn in a slanting down-to-the-right (DR) orientation. Drawing from existing studies on the influence of processing biases and prior experience on directional scanning of visual materials, the authors hypothesized that the direction of the slant influences students’ processing of the cladogram and that the more commonly used UR format is harder for students to understand.They tested this hypothesis with a study population of 19 upper-division students majoring in biology or biology-related subjects. The subjects processed a series of 24 diagonal cladograms, each paired with a rectangular-format cladogram. The diagonal cladograms varied in one of three ways: 1) UR or DR orientation, 2) forward or reverse alphabetical order of taxa labeling, and 3) the taxon topology (branching pattern). The subjects initially viewed a diagonal cladogram presented on the center of a computer screen, and their eye movements were tracked electronically. When they indicated that they understood the diagonal cladogram, they were presented with a rectangular cladogram, and then were asked whether the evolutionary relationships depicted in the two cladograms were the same. The authors used rectangular cladograms for comparison, because information about how students interpret them was available from a previous study (Novick and Catley, 2007 ). Incorrect rectangular cladograms could therefore be modeled after common types of interpretation and translation errors observed in this earlier study.Analysis of the results indicated that, for both the UR and DR orientations, the subjects tended to scan the cladograms from left to right (upward for the UR cladograms and downward for the DRs); that is, most used the processing direction that they use to read text. There was a significant effect of cladogram orientation on the accuracy of translation to the rectangular format. As predicted, the subjects were more successful at translating the diagonal cladogram to the rectangular format when the DR orientation was used. As a possible explanation for this finding, the authors suggest that people generally encounter the branching points in an order that reflects the nesting pattern when reading from left to right in the DR orientation. Thus, in this study, informationally equivalent UR and DR cladogram formats were not computationally equivalent for students.The authors conclude by discussing the implications for instruction. They suggest that if textbook diagrams do not change, students could benefit from instruction and practice in how to change their processing strategies to successfully interpret the computationally more difficult UR-oriented cladograms.2. Liben LS, Kastens K, Christensen AE (2011). Spatial foundations of science education: the illustrative case of instruction on introductory geological concepts. Cogn Instr 29, 45–87.[Abstract available: www.tandfonline.com/doi/abs/10.1080/07370008.2010.533596]The concepts of strike and dip, used to describe planar features such as the orientation of layers of rock, are notoriously difficult aspects of spatial thinking for novice learners of geology to grasp. The “strike” of a planar surface (such as a fault, bed, or other type of geologic formation) refers to the compass direction of a line of intersection of the planar surface with a horizontal plane; the latter is often referenced to the surface of a still body of water. The “dip” is the angle of tilt of the surface from the horizontal. Textbook illustrations of the strike and dip of geologic features often attempt to make the concepts more accessible by using water level and falling water to help students understand these concepts. However, educators are becoming increasingly aware that, for some students, these illustrations may convey insufficient information about three-dimensional spatial relationships. In this study, the authors used various tasks related to strike and dip to explore the nature of students’ underlying difficulties. In doing so, they anticipated that performance on stripe and dip tasks would shed light on broader issues related to spatial perception and how it influences science learning.The study population consisted of 125 college students (roughly equal numbers of males and females) who had completed a pencil-and-paper water-level test in which they drew lines of predicted water levels on diagrams of straight-sided empty bottles tilted at different angles. Participants were assigned to high-, medium-, and low-scoring water-level groups (WLG) based on their test scores. Each WLG was then assigned a series of additional field and laboratory tasks. Field tasks included estimating and recording (on a campus map) the strike and dip of an artificial rock outcrop, indicating the location of a wooden rod placed on the ground on a campus map, and additional tasks that assessed sense of direction. Laboratory tasks consisted of a series of three-dimensional horizontality (shoreline) and verticality (drop) tasks. Both sets of tasks used plastic models with paper-covered planar surfaces of different shapes attached to clear Plexiglas pillars; the dip of the surfaces varied, but the strike was held constant In the shoreline task, subjects were asked to imagine that the whole model was covered in water up to the midpoint of the paper-covered surface and to then draw on the paper how the water would look. In verticality tasks, the subjects were asked to imagine that a drop of water had fallen on the paper surface, were then asked to draw the path of the drop along the paper after it fell. Participants were asked to supply information about their level of confidence in their performance on all tasks, and observations were made of their behaviors and the strategies they used.The authors determined the variance of the absolute values by which scores on the directional responses to field and laboratory tasks deviated from the correct scores, with WLG and participant gender as between-subject factors. Although they found that students in the low WLG generally had the lowest task scores, the entire study population appeared to be challenged by the tasks. The authors used multiple regression analysis, in which confidence served as the criterion variable to determine whether the water-level “pretest,” actual performance on the field and laboratory tasks, and being female were predictive of participants’ confidence level. They found that low water-level scores and being female were predictive of low confidence scores, and performance on tasks was generally predictive of participants’ confidence ratings. In groups of students with similar scores on the water-level test, females scored lower than males on a number of the tasks. The authors speculate that the water-level test may not have identified all key components of spatial skill needed to complete the tasks, because the gender differences were most pronounced when participants had to orient in relation to a larger, more distal environment and were absent when more local frames of reference for orientation could be used. Finally, observations of the participants’ task performance indicated that they often did not use strategies that educators might assume are too basic to warrant mentioning in the course of instruction.The authors conclude that, in fact, strike and dip are difficult geological concepts to teach, and the difficulty may lie in part with underdevelopment of students’ “Euclidean conceptual system” (p. 81). They suggest the need for more research to inform the design of instructional programs that would foster development of specific foundational spatial concepts and skills.3. Fulop RM, Tanner KD (2012). Investigating high school students’ conceptualizations of the biological basis of learning. Adv Physiol Educ 36, 131–142.[Full text available: http://advan.physiology.org/content/36/2/131.long]This study sets the stage for increasing the amount and relevance of high school neuroscience education by exploring what students already know about the biological basis of learning. Recent studies (e.g., Blackwell et al., 2007 ) suggest that the nature of students’ understandings about this area of cognitive neuroscience—the biological basis of learning—has implications for their academic success.High school juniors (n = 339) enrolled in chemistry classes in a large urban high school participated in the study, which used a mixed-methods design consisting of written assessments (both multiple-choice and open-ended assessment prompts) and interviews. Although all participants were invited to participate in the interviews, only a few (n = 15) actually did so. Most of the 19 “yes/no/I don''t know” multiple-choice assessment prompts were taken from the literature, and all were demonstrated to elicit agreement from neuroscientists (>90%) on the answer. The first of the two open-ended prompts was designed to determine whether students would place the process of learning within a biological or some other framework; the second was designed to explicitly elicit responses of a biological nature. Two independent observers analyzed the interview responses by: 1) sorting them into one of three categories (nonbiological, minimally biological, or primarily biological) using a rubric; 2) scoring for the level of understanding they revealed about neural structures, mechanisms of learning, and plasticity of the nervous system using a second rubric; and then 3) coding them for emergent conceptual themes.The findings indicated a low level of knowledge about the biological basis for learning in this set of high school juniors: <70% of the students agreed with the neuroscientists’ responses to the majority of the multiple-choice prompts; 75% of the responses to the first open-ended assessment exhibited a nonbiological framework; and 67% of the interviewed subjects revealed misconceptions during the interview. The authors provide numerous quotes from students to illustrate these conclusions. Fewer than half of the interview subjects reported having had prior (albeit minimal) instruction about neuroscience, a topic that is included in the National Science Education Standards. However, the majority thought that understanding how people learn was of value to their own learning.The authors conclude by underscoring the importance to the general public of teaching about the biology of the brain, particularly since high school biology represents the last opportunity for formal education to reshape preconceptions of the >70% of the U.S. population that will not go on to college. Although the teaching of neuroscience in high school is not yet prevalent, in the words of the authors, the good news is that “students appear to be ready, willing and able to learn about their own brains” (p. 139).4. Nadelson LS, Southerland S (2012). A more fine-grained measure of students’ acceptance of evolution: development of the inventory of student evolution acceptance: I-SEA. Int J Sci Educ 34, 1637–1666.[Abstract available: www.tandfonline.com/doi/abs/10.1080/09500693.2012.702235]Science, technology, engineering, and mathematics educators and education researchers are becomingly increasingly aware of the potential role that affective constructs, such as learning dispositions, self-efficacy, beliefs, and motivation, can play in shaping the learning process. This study is based on a premise that, for emotionally charged topics such as evolution, the affective perceptions of belief and acceptance can interfere with conceptual understanding. The authors have developed an instrument for measuring students’ acceptance of biological evolution in a way that avoids blending acceptance with belief or understanding of specific content. They report having taken particular care in designing the instrument to distinguish acceptance of evolution—based on the validity of the evidence supporting it and its plausibility and utility as an explanatory paradigm—from beliefs about evolution based on feelings, personal convictions, or faith.The article guides the reader through the processes of instrument design, item and scale development, and field-testing (with groups of high school and college students) of an initial 49-item Likert-scale instrument: the Inventory of Student Evolution Acceptance (I-SEA). The instrument has three subscales designed to differentiate between areas of evolution that are perceived differently by the general public: microevolution (the results of evolution in the short term), macroevolution (long term), and human evolution. After field-testing, the authors performed statistical analyses to determine instrument and subscale reliability, as well as an exploratory factor analysis to guide instrument refinement. They conducted a refined analysis of the resulting 24-item instrument as a whole and for each of the three subscales. Ten postsecondary biology faculty contributed to the process of expert validation and final refinement of the items. The authors point out the potential usefulness of the instrument, as well as its possible limitations, in making curricular decisions and assessing their subsequent impact on student perceptions. Appendices include the items from both the field-tested and final versions of the I-SEA.I invite readers to suggest current themes or articles of interest in life science education, as well as influential papers published in the more distant past or in the broader field of education research, to be featured in Current Insights. Please send any suggestions to Deborah Allen (ude.ledu@nellaed).  相似文献   

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Insect resistance and glyphosate tolerance have been two of the most important traits in the genetic improvement of various crops. In this study, two Bacillus thuringiensis (Bt) insecticidal genes, Cry1Ac and Cry1Ig, and a modified glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene (G10) were combined into a single transferred DNA (T-DNA) fragment and introduced into rice by Agrobacterium-mediated transformation. A transgenic line with single-copy T-DNA insertion named GAI-14 was found to be highly resistant to striped stem borer and rice leaf roller, and tolerant to glyphosate. Analysis of T-DNA border sequence suggested that the transgenes were inserted at the chromosome 3 and appeared to have not interrupted any known or putative genes. A field trial observed no significant difference in the basic agronomic traits between GAI-14 and the recipient rice.  相似文献   

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Eukaryotic initiation factor subunit c(eIF3c) has been identified as an oncogene that is over-expressed in tumor cells and,therefore,is a potential therapeutic target for gene-based cancer treatment.This study was focused on investigating the effect of small interfering RNA(siRNA)-mediated eIF3c gene knockdown on colon cancer cell survival.The eIF3c gene was observed to be highly expressed in colon cancer cell models.The expression levels of the gene in eIF3c siRNA infected and control siRNA infected cells were compared via real-time polymerase chain reaction(PCR) and western blotting analysis.Cell proliferation levels were analyzed employing 3-(4,5-dimethylthiazol 2-yl)-2,5-diphenyltetrazolium bromide(MTT) and colony formation assays.Furthermore,the effects of eIF3c gene knockdown on the cell cycle and apoptosis were analyzed using flow cytometry.The results showed that suppression of eIF3c expression significantly(P<0.001) reduced cell proliferation and colony formation of RKO colon cancer cells.The cell cycle was arrested by decreasing the number of cells entering S phase.Further,apoptosis was induced as a result of eIF3c knockdown.Collectively,eIF3c deletion effectively reduced the survival of colon cancer cells and could be used as a therapeutic tool for colon cancer therapy.  相似文献   

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The gene AtCSR encodes peptidyl-prolyl cis/trans isomerases (PPIases) that accelerate energetically unfavorable cis/trans isomerization of the peptide bond preceding proline production.In our studies,we found that AtCSR was associated with cadmium (Cd)-sensitive response in Arabidopsis.Our results show that AtCSR expression was triggered by Cd-stress in wild type Arabidopsis.The expression of some genes responsible for Cd2+ transportation into vacuoles was induced,and the expression of the iron-regulated transporter 1 (IRT1) related to Cd2+ absorption from the environment was not induced in wild type with Cd2+ treatment.The expression of Cd-transportation related genes was not in response to Cd-stress,whereas IRT expression increased dramatically in atcsr-2 with Cd2+ treatment.The expression of glutathione 1 (GSH1) was consistent with GSH being much lower in atcsr-2 in comparison with the wild type with Cd2+ treatment.Additionally,malondialdehyde (MDA),hydrogen peroxide,and Cd2+ contents,and activities of some antioxidative enzymes,differed between the wild type and atcsr-2.Hydrogen sulfide (H2S) has been confirmed as the third gas-transmitter over recent years.The findings revealed that the expression pattern of H2 S-releasing related genes and that of Cd-induced chelation and transportation genes matched well in the wild type and atcsr-2,and H2S could regulate the expression of the Cd-induced genes and alleviate Cd-triggered toxicity.Finally,one possible suggestion was given:down-regulation of atcsr-2,depending on H2S gas-transmitter not only weakened Cd2+ chelation,but also reduced Cd2+ transportation into vacuoles,as well as enhancing the Cd2+ assimilation,thus rendering atcsr-2 mutant sensitive to Cd-stress.  相似文献   

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