共查询到19条相似文献，搜索用时 359 毫秒
1.
嗜酸乳杆菌高密度发酵条件的研究 总被引：2，自引：0，他引：2
主要研究了嗜酸乳杆菌生长繁殖的环境条件(温度、接种量、起始pH等)和培养基组成对其影响.优化后确定了嗜酸乳杆菌的高密度培养条件为:起始pH值为5.8,培养温度为37℃,培养条件为亨-盖特厌氧培养,接种量为4%,培养时间为22 h.并且筛选了碳源,氮源及氮源的浓度.基配比为:2%乳清粉(w/v),1.5%牛肉膏(w/v),1.5%蛋白胨(w/v),0.058%硫酸镁(w/v),0.025%硫酸锰(w/v),0.22%乙酸钠(w/v),0.2%磷酸氢二钾(w/v).将该菌在此增菌培养基上培养,在37℃下培养22 h,菌数可达到1.17×1011cfu/mL. 相似文献
2.
3.
4.
以大头菜为原料腌制泡菜,研究其发酵过程中亚硝酸盐含量的变化,通过调整腌渍液的起始pH、食盐添加量、Vc添加量及发酵温度,并采用人工接种乳酸菌的方法研究控制泡菜发酵过程中亚硝酸盐的含量。 相似文献
5.
基于模糊综合评判-响应面分析的苹果酒发酵工艺参数优化 总被引：1，自引：0，他引：1
李坤娜 《内蒙古科技与经济》2018,(2)
选用原生质体融合法构建的优良苹果酒酵母菌株S.cerevisiae21#,通过Box-Behnken实验设计,研究了苹果酒发酵工艺参数:温度、发酵液pH值、接种量对苹果酒品质的影响,以苹果酒品质模糊综合评判结果为响应值,建立了苹果酒品质与发酵工艺参数的二次多项式数学模型,通过模型的响应面分析,结果表明:S.cerevisiae21#酵母菌株酿造苹果酒的最佳发酵工艺参数为:发酵温度21.3℃、pH 3.36、接种量8.24%。 相似文献
6.
7.
利用固体发酵工艺生产绿色木霉孢子粉的研究 总被引：1，自引：0，他引：1
《黑龙江科技信息》2016,(2)
通过单因素发酵实验与正交实验,以麸皮、稻壳等农业废弃物为固体培养基,开展木霉固体发酵工艺研究。结果表明:绿色木霉M21最佳固体发酵培养基配方:麸皮58.86%,稻壳37.9%,硫酸铵1.5%,葡萄糖1%,KH2PO40.2%,Mg SO40.04%,Ca CO30.5%。菌株培养温度30℃,装料量1:10,接种量8%,培养时间5天,孢子产量为9.7*109个/克,为绿色木霉生产应用提供参考。 相似文献
8.
青海地处高原高寒干燥地区,其农作物生产加工较内地仍为匮乏,尤其是农产品深加工环节仍较为薄弱。为拓宽生产渠道,扩大青海高原特色农产品——藜麦、青稞的社会、经济和市场效益,为藜麦青稞作物再加工和青海酸乳的发展开辟新的空间。本研究以开发高品质藜麦-青稞酸奶工艺为主要目的,通过研究藜麦-青稞料液比、接种量、发酵时间和发酵温度对藜麦复合酸奶品质的影响,由单因素试验获得了不同工艺参数条件下的藜麦复合型酸乳,通过正交分析方法获得了最优工艺参数,并进行了定性定量评价。研究表明:随着料液比、接种量、发酵时间、发酵温度的增加或升高,在一定条件下,藜麦复合型酸乳的感官评分呈现先升高后下降的趋势,最适添加量藜麦15%、青稞5%,最适发酵温度40℃,最适发酵时间6h;结合正交试验最后得出该藜麦复合型酸乳的最佳工艺参数为:添加量藜麦10%、青稞5%,接种量3%,发酵时间6h,发酵温度40℃下制备的酸奶具有藜麦酸乳特有的风味,白色或微带浅黄色、光滑细腻、组织状态、滋味俱佳。 相似文献
9.
具有抑菌活性乳酸菌发酵条件的研究 总被引：1，自引：0，他引：1
以致病菌大肠杆菌、单细胞里斯特氏菌、金黄色葡萄球菌为指示菌,以乳酸菌SMN3-3为试验菌株,研究发酵条件对菌株SMN3-3发酵乳上清液抑菌效果的影响,试验结果发现,乳酸菌SMN3-3以5×106CFU/mL为接种量,37℃发酵24h,得到的发酵乳上清液的抑菌效果为最佳,结果表明,不同的发酵条件对乳酸菌SMN3-3抑菌效果有很大影响。 相似文献
10.
11.
BackgroundThere is a large amount of industrial wastewater produced by the mushroom industry during the canning processing each year, which could provide abundant carbon, nitrogen and inorganic salts for microbial growth. The aim of this study was to optimize the culture conditions for Bacillus licheniformis cultured in the Agaricus bisporus industrial wastewater to produce the agricultural microbial fertilizer.ResultsIn this work, the maximal biomass of B. licheniformis could be obtained under the following culture conditions: 33.7°C, pH 7.0, 221 rpm shaking speed, 0.5% wastewater, 2 (v:v, %) inoculum dose, loading liquid of 60 mL/250 mL and a culture time of 24 h, and the average experimental value obtained was 1.35 ± 0.04 × 109 Obj/mL, which was within the 95% confidence interval of the predicted model (1.29–1.38 × 109 Obj/mL), and met the national microbial fertilizers' standard in China. Furthermore, the field experiment results showed that the fermentation broth of B. licheniformis could significantly improve the yield of Anoectochilus roxburghii.ConclusionsAgaricus bisporus industrial wastewater can be used to produce agricultural microbial fertilizer.How to cite: Huang J, Huang A, Lu L, et al. Improving the yield of Anoectochilus roxburghii by Bacillus licheniformis cultured in Agaricus bisporus industrial wastewater. Electron J Biotechnol 2020;48. https://doi.org/10.1016/j.ejbt.2020.08.002. 相似文献
12.
BackgroundCellulose as a potential feed resource hinders its utilization because of its complex structure, and cellulase is the key to its biological effective utilization. Animal endogenous probiotics are more susceptible to colonization in the intestinal tract, and their digestive enzymes are more conducive to the digestion and absorption of feed in young animals. Min pigs are potential sources of cellulase probiotics because of the high proportion of dietary fiber in their feed. In this study, the cellulolytic bacteria in the feces of Min pigs were isolated and screened. The characteristics of enzymes and cellulase production were studied, which provided a theoretical basis for the rational utilization of cellulase and high-fiber food in animal production.ResultsIn our study, 10 strains of cellulase producing strains were isolated from Min pig manure, among which the M2 strain had the best enzyme producing ability and was identified as Bacillus velezensis. The optimum production conditions of cellulase from strain M2 were: 2% inoculum, the temperature of 35°C, the pH of 5.0, and the liquid loading volume of 50 mL. The optimum temperature, pH and time for the reaction of cellulase produced by strain M2 were 55°C, 4.5 and 5 min, respectively.ConclusionsMin pigs can be used as a source of cellulase producing strains. The M2 strain isolated from feces was identified as Bacillus velezensis. The cellulase from M2 strain had a good activity and the potential to be used as feed additive for piglets.How to cite: Li F, Xie Y, Gao X, et al. Screening of cellulose degradation bacteria from Min Pigs and optimization of its cellulase production. Electron J Biotechnol 2020;48. https://doi.org/10.1016/j.ejbt.2020.09.001 相似文献
13.
利用SBR亚硝化处理化肥厂实际废水,研究其短程生物脱氮过程中pH、溶解氧、进水氨氮负荷和温度等因素影响,并确定亚硝化处理的最佳操作条件。结果表明,pH过低会抑制亚硝酸盐的生成,过高则不利于反硝化菌反硝化过程TN的去除;当pH值控制在8.0左右时,亚硝化率保持较高水平,同时出水TN浓度控制较好。当DO浓度为0.2~0.3 mg/L时,亚硝化反应继续进行,但NH4+-N亚硝化反应速率较慢;当DO浓度为1.5~2.8 mg/l时,无法实现系统中亚硝化的运行,硝化作用成为主要反应。高氨氮负荷进水有利于亚硝酸盐的积累,但对出水氨氮去除效果不佳。较高的温度有利于亚硝化反应进行。当t=35℃,初始pH控制在7.8~8.2,DO控制在0.5~0.6mg/L,进水浓度NH4+-N为100mg/L时,SBR亚硝化操作过程为最佳,此时亚硝化率基本稳定在90%以上。 相似文献
14.
BackgroundThe potential waste canola oil-degrading ability of the cold-adapted Antarctic bacterial strain Rhodococcus sp. AQ5-07 was evaluated. Globally, increasing waste from food industries generates serious anthropogenic environmental risks that can threaten terrestrial and aquatic organisms and communities. The removal of oils such as canola oil from the environment and wastewater using biological approaches is desirable as the thermal process of oil degradation is expensive and ineffective.ResultsRhodococcus sp. AQ5-07 was found to have high canola oil-degrading ability. Physico-cultural conditions influencing its activity were studied using one-factor-at-a-time (OFAT) and statistical optimisation approaches. Considerable degradation (78.60%) of 3% oil was achieved by this bacterium when incubated with 1.0 g/L ammonium sulphate, 0.3 g/L yeast extract, pH 7.5 and 10% inoculum at 10°C over a 72-h incubation period. Optimisation of the medium conditions using response surface methodology (RSM) resulted in a 9.01% increase in oil degradation (87.61%) when supplemented with 3.5% canola oil, 1.05 g/L ammonium sulphate, 0.28g/L yeast extract, pH 7.5 and 10% inoculum at 12.5°C over the same incubation period. The bacterium was able to tolerate an oil concentration of up to 4.0%, after which decreased bacterial growth and oil degradation were observed.ConclusionsThese features make this strain worthy of examination for practical bioremediation of lipid-rich contaminated sites. This is the first report of any waste catering oil degradation by bacteria originating from Antarctica.How to cite: Ibrahim S, Zahri KNM, Convey P, et al. Optimisation of biodegradation conditions for waste canola oil by cold-adapted Rhodococcus sp. AQ5-07 from Antarctica. Electron J Biotechnol 2020;48. https://doi.org/10.1016/j.ejbt.2020.07.005 相似文献
15.
Jerry Joseph Emanuel Dickson M. B. Kadam P. K. Joseph 《Indian journal of clinical biochemistry : IJCB》1991,6(1):27-29
Rats were fasted for 8 hours and then fed 3 ml of 25% (v/v) ethanol per 100g body weight and subsequently sacrificed 18 hours
later. The levels of triacylglycerols and total cholesterol were significantly raised in serum and liver of alcohol fed rats.
However, when rats were fed on aqucous extract of onions (300 mg per 100g body weight) with 25% ethanol, no rise in serum
and liver lipids was observed. Alcohol fed to the fats with or without onion extract had no effect on serum, AST, ALT and
alkaline phosphatase levels. The increase in serum urea in ethanol-fed rats was not altered in rats fed a mixture of alcohol
and onion extracts. Values of liver MDA was lower in rats fed ethanol with or without onion extract compared to controls.
Onion extract seemed to show a hypolipidemic effect in alcohol fed rats. 相似文献
16.
本文在分析污泥膨胀的发生机理的前提下,阐述引起污泥膨胀的主要原因：污泥负荷、溶解氧、pH值、温度等;并提出控制污泥膨胀的一些措施：调节反应池中的溶解氧及pH值,投加营养盐合理调配营养比等。 相似文献
17.
BackgroundBiotechnological processes are part of modern industry as well as stricter environmental requirements. The need to reduce production costs and pollution demands for alternatives that involve the integral use of agro-industrial waste to produce bioactive compounds. The citrus industry generates large amounts of wastes due to the destruction of the fruits by microorganisms and insects together with the large amounts of orange waste generated during the production of juice and for sale fresh. The aim of this study was used orange wastes rich in polyphenolic compounds can be used as source carbon of Aspergillus fumigatus MUM 1603 to generate high added value compounds, for example, ellagic acid and other molecules of polyphenolic origin through submerged fermentation system.ResultsThe orange peel waste had a high concentration of polyphenols, 28% being condensed, 27% ellagitannins, 25% flavonoids and 20% gallotannins. The major polyphenolic compounds were catechin, EA and quercetin. The conditions, using an experimental design of central compounds, that allow the production of the maximum concentration of EA (18.68 mg/g) were found to be: temperature 30°C, inoculum 2 × 107 (spores/g) and orange peel polyphenols 6.2 (g/L).ConclusionThe submerged fermentation process is an effective methodology for the biotransformation of molecules present in orange waste to obtain high value-added as ellagic acid that can be used as powerful antioxidants, antibacterial and other applications.How to cite: Sepúlveda L, Laredo-Alcalá E, Buenrostro-Figueroa JJ, et al. Ellagic acid production using polyphenols from orange peel waste by submerged fermentation. Electron J Biotechnol 2020;43. https://doi.org/10.1016/j.ejbt.2019.11.002. 相似文献
18.
为探索真菌对染料的脱色特性,利用分离得到的短刺小克银汉霉对偶氮染料刚果红进行了脱色研究. 短刺小克银汉霉菌丝球能在3h内使刚果红脱色率达到96%以上. 在通气脱色体系中研究了短刺小克银汉霉菌丝球在不同pH值、脱色温度、摇床转速、盐度等条件下对刚果红脱色效果的影响. 研究结果表明,短刺小克银汉霉菌丝球在温度为33℃,pH6.5,摇床转速120r/min的条件下对刚果红具有最大脱色率,染料溶液中盐浓度对脱色率有一定影响,但影响不大. 在刚果红50~200mg/L质量浓度范围内,小克银汉霉菌丝球对刚果红吸附脱色动力学符合拟二级动力学方程(R2>0.999). 菌丝球对刚果红的吸附等温线可用Langmuir和Freundlich 等温方程模型表达,其中Langmuir方程能更好地描述菌丝球对染料的吸附行为(R2>0.999). 相似文献
19.
《Electronic Journal of Biotechnology》2014,17(6):262-267
BackgroundThe Tibetan pig is a pig breed with excellent grazing characteristics indigenous to the Qinghai–Tibet plateau in China. Under conditions of barn feeding, 90% of its diet consists of forage grass, which helps meet its nutritional needs. The present study aimed to isolate and identify a cellulolytic bacterium from the Tibetan pig's intestine and investigate cellulase production by this bacterium. The study purpose is to provide a basic theory for the research and development of herbivore characteristics and to identify a source of probiotics from the Tibetan pig.ResultsA cellulolytic bacterium was isolated from a Tibetan pig's intestine and identified based on morphological, physiological, and biochemical characteristics as well as 16S rRNA analysis; it was designated Bacillus subtilis BY-2. Examination of its growth characteristics showed that its growth curve entered the logarithmic phase after 8–12 h and the stable growth phase being between 20 and 40 h. The best carbon source for fermentation was 1% corn flour, while 2% peptone and yeast powder compound were the best nitrogen sources. The initial pH during fermentation was 5.5, with 4% inoculum, resulting in a high and stable amount of enzyme in 24–48 h.ConclusionsThe isolated BY-2 strain rapidly grew and produced cellulase. We believe that BY-2 cellulase can help overcome the shortage of endogenous animal cellulase, improve the utilization rate of roughage, and provide strain sources for research on porcine probiotics. 相似文献