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1.
以黑皮鸡枞菌深层液体发酵菌丝体生物量为指标,首先通过单因素试验确定了最佳的培养温度、摇床转速、pH值、发酵周期,然后经过响应面试验的优化,得出最佳发酵条件:培养温度为26℃,摇床转速为175 r/min,pH值为7.6,发酵周期为25 d.通过验证培养后,黑皮鸡枞菌丝体干重达6.91 mg/mL.  相似文献   

2.
研究了羊肚菌深层发酵培养基中玉米粉和黄豆粉配比和发酵条件,确定了羊肚菌深层发酵最佳配比的培养基以及最佳的发酵温度、起始pH和摇床转速.通过单因素确定培养基中玉米粉和黄豆粉的最佳配比,在单因素试验的基础上采用三因素三水平的响应面分析法确定羊肚菌液体发酵的培养条件.结果以羊肚菌菌丝体生物量和胞外多糖浓度为响应面和等高线,在分析各个因素的显著性和交互作用后,得出羊肚菌液体发酵的最佳温度25.33℃、起始pH 6.13、摇床转速154.33r/min.在最佳发酵条件下,发酵144h,羊肚菌的菌丝体生物量和胞外多糖浓度达到最高,分别为7.78g/L和130.97mg/L.  相似文献   

3.
通过摇瓶培养法对真姬菇液体发酵产胞外多糖进行研究,得到产胞外多糖适宜的条件为:真姬菇液体发酵产胞外多糖的最适时间是8单位,最适pH为6.5,的最适温度为26℃、摇瓶转速为130r/min、最适装液量为50%、最适接种量为15%.  相似文献   

4.
以菌丝体干重为指标,通过单因子试验和多因子正交试验,研究蘑菇液体发酵的最佳外界条件。结果表明,蘑菇液体发酵培养的最适碳源为3%可溶性淀粉,最适氮源为0.3%牛肉膏;其他最佳发酵条件为:摇瓶转速130r·min-1,温度25℃,接种量为15%(V/V),最佳发酵时间为7d。  相似文献   

5.
几种食用真菌对甘蔗渣利用的研究   总被引:1,自引:0,他引:1  
采用液体摇床发酵和固体平板培养技术,以甘蔗渣为唯一碳源,研究灵芝、大球盖菇、金针菇、柱状田头菇四种食用真菌对甘蔗渣的利用情况.测定在四种不同甘蔗渣含量的培养基中各食用真菌所产羧甲基纤维素酶活力和菌丝干重,同时测定了四种茵在平板上的菌丝生长速度.结果表明,灵芝在35g/L液体培养基中所产羧甲基纤维素酶活力最高达831U,所生长茵丝最重达1.162g.固体培养时,金针菇在30g/L生长速度最快达15mm/d.  相似文献   

6.
金针菇菌丝体液体培养研究   总被引:3,自引:0,他引:3  
金针菇菌丝在不同配比的葡萄糖,蛋白胨液体培养基中有不同的生长速率,在24℃下以110 ̄120r/min的摇床转速进行的培养试验表明,金针菇菌丝合适的液体培养条件为:葡萄糖3%、蛋白胨0.3%,MgSO4 0.05%,KH2PO4 0.1%,VB1 10mg/L,PH6,培养时间7天,菌丝体最终浓度达到0.45g/100ml(菌丝干重)。  相似文献   

7.
从商丘市不同环境的土壤中分离筛选到几株产脂肪酶的霉菌菌株,作者对其中的一株根霉(Rhizopus delemar)进行了液体发酵的研究,使用发酵产酶培养基组成(%):豆粉6.0,蛋白胨4.0,蔗糖1.0,K2HSO4 0.2,MgSO4·7H2O0.1,pH6.5.产酶最适温度28 ℃,接种量为1 ml,培养周期48 h,转速200 r/min.  相似文献   

8.
采用液体摇床发酵和固体平板培养技术,以甘蔗渣为唯一碳源,研究灵芝、大球盖菇、金针菇、柱状田头菇四种食用真菌对甘蔗渣的利用情况。测定在四种不同甘蔗渣含量的培养基中各食用真菌所产羧甲基纤维素酶活力和茵丝干重,同时测定了四种茵在平板上的茵丝生长速度。结果表明,灵芝在35g/L液体培养基中所产羧甲基纤维素酶活力最高达831U,所生长茵丝最重达1.162g。固体培养时,金针菇在30g/L生长速度最快达15mm/d。  相似文献   

9.
碳源、氮源及其比例对香菇液体深层培养的影响   总被引:1,自引:0,他引:1  
通过摇瓶培养,研究香菇液体深层培养最适发酵培养时间、最适碳源、最适氮源及最佳碳源与氮源质量配比.结果表明最适发酵培养时间为168 h,所得菌丝体干重达0.399 g/50 mL菌液,最适碳源为蔗糖,菌丝体干重达到0.396 g/50 mL菌液;最适氮源为牛肉膏,菌丝干重达到0.421 g/50 mL菌液,并通过正交实验筛选出最佳碳源与氮源质量配比为:W(蔗糖):W(牛肉膏):W(麦芽糖)=2.5:1:1.  相似文献   

10.
张海英  李昙 《德州学院学报》2013,29(4):65-68,73
目的:从活性污泥中分离出株絮凝剂细菌,并优化其培养条件.方法:经过初筛和复筛得到获得1株产高效MBF的菌种,编号为F1.以菌株F1为研究对象,测定其发酵液对高岭土悬浊液的絮凝率.结果:该菌产絮凝剂的适宜碳源为可溶性淀粉,适宜氮源为蛋白胨,当初始pH值7.0,温度为35℃,摇床转速为120r/min,培养时间72h,在最适培养条件下其对高岭土悬浊液的絮凝率均达到90%以上.  相似文献   

11.
《海外英语》2007,(5):44-45
It is worthy of noting that, whilst Crookston Castle witnessed the earlier and happier portion of Mary's variegated life,  相似文献   

12.
一、吃和喝吃苹果 eat an apple, 吃药 take medicine,吃糖 have some sweets,吃饭 have one's meals,吃馆子 dine out,吃惊 be surprised/  相似文献   

13.
《海外英语》2007,(5):10-11
Many college freshmen arrive woefully unprepared to do college work, and as disadvantaged populations continue to grow, the share of the American work force that has made it through college is expected to plummet. Many experts blame that educational failure not just on high schools but also on colleges. School & College, a special report by The Chronicle, looks at efforts to fix the system. What reforms would better prepare students for college? What should schools and colleges be doing differently? How should state and federal officials help?  相似文献   

14.
汉字倒说     
汉字的六书,《说文》对“转注”一类,语焉不详。后世学者提及转注,也仅限于许慎所举出的例字。《汉字例说》一文,作者从转注的角度综合考虑,对部分现代常用字作了分析,跟传统的解释有所不同。希望能抛砖引玉,互相切磋,以推进学术研究。  相似文献   

15.
《海外英语》2007,(4):36
There are numbers of crossroads on our long and unpredictable life journey where we totally have no idea about which direction to choose. No matter what our decision is, we should not turn back, but face the music and go ahead instead. I am this kind of girl who always does try without regretting, one example is how I dealt with my love.  相似文献   

16.
王菲 《华章》2007,(12):273-273
Migration occurs behind a variety of reasons and has a great effect on the whole world. People may migrate in order to improve their economic situation, or in order to escape civil strife, persecution, and environmental disasters. The impact of migration is complex, bringing both benefits anddisadvantages. This paper briefly talks about the causes of migration, the allocation of benefits, and the ways in which individual countries and the international community deal with this important subject.  相似文献   

17.
裴水妹 《华章》2007,(11):196
Sister Carrie is one of the most controversial characters in American literature.Thought as a "fallen woman" firstly,she was defined as a "new woman" by some critics later. However, by digging into the motivaton behind the whole process of Carrie's "success", the relationship between Carrie and her creator (the author), the social conditions of then American, it can be found that Carrie has never been free-standing on her thought and she has never found her real-sdf even after becoming a famous actress. In a society dominated by mass consumerism Carrie is only an adherent of her own desires. She also is a representative of all those country girls flooded into cities, a symbol and a sacrifice of the urbanization of America in a time countryside was overcome by cities.  相似文献   

18.
19.
1.IntroductionOne-cyclecontrolmethod,whichwasproposedaboutonedecadeago[1],hasbecomeanattractivemethodinspecialfieldssuchaspowerfactorcorrection[2-6],switchingamplifiers[7,8],etc.Themainideaofthiscontrollerisbasedonintegrationofdiodevoltageinone-cycleandforcesittobeexactlyequaltothereferencevalue.Themainadvantageofthiscontrollerisitsrealtimeabilitytorejectthevariationofinputvoltage[1].Despitethisgreatability,ithasnogoodperformancesinrejectingofloaddisturbanceandfollowingreferencecommands.Espec…  相似文献   

20.
钱生钱!     
我们绝大部分人都同意一件事情——我们喜欢金钱。但是你对金钱的喜欢之情足够到让你创立一份事业么?.财富通常被人忽视,主要在于人们不能真正了  相似文献   

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