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1.
小西葫芦黄化花叶病毒(zucchini yellow mosaic virus,ZYMV)和黄瓜花叶病毒(cucumber mosaicvirus.CMV)是侵染葫芦科植物的主要病毒.本研究在三种供试品种的瓠瓜上单独和混合接种ZYMV和CMV,对各处理的病情和病毒积累量进行分析.两种病毒在三种瓠瓜供试品种上均表现明显的致病性.ZYMV和CMV的复合侵染加重瓠瓜发病症状,表现为协生作用.应用酶联免疫吸附法(ELISA)对三种瓠瓜上ZYMV与CMV进行检测并分析了病毒的积累量.结果表明,复合侵染情况下CMV的积累高于单独侵染,与致病性检测结果一致. 相似文献
2.
Seema Pavaman Sindgikar Suchetha Rao Rathika D. Shenoy Nutan Kamath 《Indian journal of clinical biochemistry : IJCB》2013,28(1):95-97
Propionic acidemia (PA), an uncommon organic acidemia has varied clinical and metabolic presentation causing difficulty and delay in the diagnosis. We report a case of PA in an infant who presented with failure to thrive, acute encephalopathy due to severe hyperammonemia without acidosis and fungal sepsis. The biochemical basis of severe hyperammonemia is discussed. 相似文献
3.
Lead poisoning presents a common acquired as well as congenital environmental threat to children’s health today. An unusual case of severe lead poisoning in breast fed male infant is presented here. The objective of the study is to describe a patient who developed clinical lead intoxication with an uncommon source of poisoning. A 6 months old male baby presented with gradual loss of weight, not feeding well and persistent vomiting. Laboratory investigation revealed that he was having anemia (Hb level 5.4 gm/dl), abnormal liver enzymes (including elevated transaminase activity) and high blood lead value (83 μg/dl). RBC morphology showed basophilic stippling with cabot ring, suggestive of a case of lead poisoning. A course of chelation treatment using calcium versenate (EDTACaNa2) was prescribed following which a radical solution for mobilization of lead from his systems was observed. 相似文献
4.
Alpha-1-antitrypsin deficiency is a hereditary disease leading to hepatitis and cirrhosis. It is the most common genetic cause
of liver disease in children which must inherit the tendency from both parents to develop. It acquires the highest priority
in the differential diagnosis in a child with chronic liver disease. In this case report we substantiate the role of serum
protein electrophoresis, in diagnosing alpha-1-antitrypsin deficiency. 相似文献
5.
目的:对独活寄生汤联合来氟米特片治疗类风湿关节炎的临床疗效进行观察。方法:对独活寄生汤联合来氟米特片治疗类风湿关节炎(肝肾亏虚型)的临床疗效进行回顾性分析;应用随机数字表把纳入的120例患者随机分为治疗组和对照组,两组各60例,对照组给予来氟米特片治疗;治疗组予以独活寄生汤联合来氟米特片治疗,1疗程为6个月,分别于治疗前、治疗1个月、2个月、3个月对患者进行DAS-28评分并采用HAQ健康评估问卷进行生存质量评估。结果:治疗组总有效率、显效率分别为88.33%和80%;对照组总有效率、显效率分别为73.33%和61.61%。总有效率、显效率治疗组均显著优于对照组。两组患者HAQ评分、DAS-28评分在治疗1个月均有所改善,病情得到控制;两组患者HAQ评分、DAS-28评分在2个月后仍继续减少,病情初步缓解,较本组治疗前有显著性差异(P0.05),组间相比仍无显著性差异(P0.05);两组患者在治疗结束时HAQ评分、DAS-28评分明显减少,病情完全控制,较本组治疗前及治疗1个月有显著性差异(P0.05),组间相比均有显著性差异(P0.05)。结论:独活寄生汤联合来氟米特片可有效改善肝肾亏虚型型类风湿关节炎患者的临床症状及实验室指标,且无明显不良反应,提高生存质量,取得了满意效果。 相似文献
6.
Barbka Repic Lampret Simona Murko Marusa Debeljak Mojca Zerjav Tansek Petja Fister Tadej Battelino 《Biochemia medica : ?asopis Hrvatskoga dru?tva medicinskih biokemi?ara / HDMB》2015,25(2):279-284
Background
Short-chain acyl-CoA dehydrogenase deficiency (SCADD) is a rare inherited mitochondrial fatty acid oxidation disorder associated with variations in the ACADS (Acyl-CoA dehydrogenase, C-2 to C-3 short chain) gene. SCADD has highly variable biochemical, genetic and clinical characteristics. Phenotypes vary from fatal metabolic decompensation to asymptomatic individuals.Subject and methods
A Romani boy presented at 3 days after birth with hypoglycaemia, hypotonia and respiratory pauses with brief generalized seizures. Afterwards the failure to thrive and developmental delay were present. Organic acids analysis with gas chromatography-mass spectrometry (GS/MS) in urine and acylcarnitines analysis with liquid chromatography-tandem mass spectrometry (LC-MS/MS) in dried blood spot were measured. Deoxyribonucleic acid (DNA) was isolated from blood and polymerase chain reactions (PCRs) were performed for all exons. Sequence analysis of all exons and flanking intron sequences of ACADS gene was performed.Results
Organic acids analysis revealed increased concentration of ethylmalonic acid. Acylcarnitines analysis showed increase of butyrylcarnitine, C4-carnitine. C4-carnitine was 3.5 times above the reference range (<0.68 µmol/L). Confirmation analysis for organic acids and acylcarnitine profile was performed on the second independent sample and showed the same pattern of increased metabolites. Sequence analysis revealed 3-bp deletion at position 310-312 in homozygous state (c.310_312delGAG). Mutation was previously described as pathogenic in heterozygous state, while it is in homozygous state in our patient.Conclusions
In our case clinical features of a patient, biochemical parameters and genetic data were consistent and showed definitely SCAD deficiency.Key words: SCAD deficiency, short chain acyl-CoA dehydrogenase deficiency, screening, acylcarnitine, polymorphism, genetic 相似文献7.
Fadhaa A. Ghafil Bassim I. Mohammad Hussain S. Al-Janabi Najah R. Hadi Hayder A. Al-Aubaidy 《Indian journal of clinical biochemistry : IJCB》2021,36(1):81
Genetic variation in the angiotensin II type 1 receptor (AT1R) has an important effect on the outcome of acute coronary syndrome (ACS) initiated treatment with captopril. This study aims to investigate the impact of genetic polymorphism of AT1R (rs5186 and rs275651) on the ACS outcome in Iraqi patients treated with captopril. A total of 250 Iraqi individuals with ACS were included in this case—control study and they were divided into two study groups; Study group 1 included 125 participants who were prescribed captopril, 25 mg twice daily and study group 2 included 125 participants who received no captopril as part of their ACS treatment (control study). The AT1R gene (rs5186) CC genotype was found to be associated with ST-elevation myocardial infarction (STEMI) (Odd’s ratio (O.R) = 1.2, P = 0.7), while AC was associated with Non-ST-elevation myocardial infarction (NSTEMI) and unstable angina (UA) (O.R = 1.2, P = 0.8). AC genotype is more prone to have Percutaneous coronary intervention (PCI) after ACS attack (O.R = 1.2, P = 0.6). CC genotype had a risk to get less improvement (O.R = 1.6, P = 0.5), so might require higher doses of captopril during acute coronary insult. The AT1R gene (rs275651) AA genotype was associated with UA (O.R = 1.3, P = 0.9). AA and AT genotypes were more prone to have PCI after ACS attack (O.R = 3.9 P = 0.2, O.R = 3.5, P = 0.3 respectively) and thus requiring higher doses of captopril. We conclude that the AT1R rs5186, rs275651 genetic polymorphisms might partially affect the clinical outcome of ACS patients treated with captopril and might have captopril resistance which requires higher doses. 相似文献
8.
Neelima Verma Jyoti chakraverty Pankaj Baweja Alka Girotra Leena Chatterjee Manish Chugh 《Indian journal of clinical biochemistry : IJCB》2017,32(1):117-120
Hyperferritinemia (>10,000 ng/ml) is an important hallmark used as an indicator of infection triggered macrophage activation syndrome leading to hemophagocytic lympho histiocytosis (HLH). Measurement of serum ferritin can be used in diagnosis as well as disease monitoring indicator and prognosis related to HLH, cAPS, sepsis, neoplasm and inflammatory conditions. It is a major contributor to manage critically ill patients as predicting and monitoring indicator. It can be used as acute phase response in conditions of MAS, AOSD, cAPS etc. A case study in our hospital showed extremely high ferritin values along with low hemoglobin, elevated LDH and triglycerides with positive MRSA in sputum culture and macrophage proliferation and hemophagocytosis in the bone marrow examination. Patient showed definite inverse relation with steroid therapy and serum ferritin levels. 相似文献
9.
Sudhesna Mohapatra Teresa Biswas Saswati Das Alpana Saxena 《Indian journal of clinical biochemistry : IJCB》2014,29(1):112-113
Alkaptonuria or ochronosis is a rare inborn disorder of metabolism which is characterized by deficiency of homogentisic acid oxidase. There is accumulation of homogentisic acid in the connective tissues causing brownish black pigmentation and skeletal damage. The most serious complication of this disease is crippling degenerative arthropathy which presents in late years of life. There is no definitive treatment for the condition. Symptomatic management is the main stay. Surgical management such as arthroplasty is done for severe cases of ochronosis. We report a case of ochronosis of the hip joint presenting with inflammation and lytic lesion which can create confusion and lead to error in diagnosis. 相似文献
10.
Sadegh Fattahi Mohammad Karimi Alivije Farhang Babamahmoodi Masomeh Bayani Mahmoud Sadeghi Haddad Zavareh Mohsen Asouri Maryam Lotfi Galia Amirbozorgi Haleh Akhavan-Niaki 《Indian journal of clinical biochemistry : IJCB》2018,33(4):467-472
Hepatitis B virus (HBV) infection is a worldwide health concern which is associated with significant morbidity and mortality. Both viral and host factors have a significant effect on infection, replication and pathogenesis of HBV. The aim of this study was to investigate the effect of CYP2E1 and CYP1A1 genetic variants on susceptibility to HBV. 143 individuals including 54 chronic HBV patients and 89 healthy controls were enrolled in the genotyping procedure. rs2031920 and rs3813867 at CYP2E1 as well as rs4646421 and rs2198843 at CYP1A1 loci were studied in all subjects using PCR–RFLP (restriction fragment length polymorphism) analysis. Both variants at CYP2E1 locus were monomorphic in all studied subjects. Genotype frequency of rs4646421 was significantly different between chronic HBV patients and healthy blood donors (P = 0.04, OR 4.31; 95% CI 1.04–17.7). Furthermore, individuals carrying at least one C allele (CC or CT genotypes) for rs4646421 seemed to have a decrease risk of hepatitis in comparison with TT genotype (P = 0.039). Our results showed a relationship between rs4646421 TT genotype (rare genotype) and the risk for developing chronic HBV infection (four times higher). Further studies are needed to examine the role of CYP1A1 polymorphism in susceptibility to chronic HBV infection. 相似文献
11.
目的:观察逍遥散加减治疗肝着肝郁脾虚证的临床疗效。方法:将80例肝着肝郁脾虚证患者随机分为两组,其中对照组40例,治疗组40例。对照组予西医护肝治疗,治疗组在西医护肝治疗基础上加用逍遥散治疗,观察比较两组临床疗效。结果:两组患者临床症状及中医证候积分均得到有效改善,治疗组临床疗效总有效率为87.50%,高于对照组67.50%(P0.05),两组中医证候积分对照组9.03±3.95,治疗组3.84±2.67,治疗组明显优于对照组(P0.05)。结论:逍遥散具有疏肝解郁、健脾和中的功效,对肝着肝郁脾虚证患者的临床症状改善有确切的疗效,并具有安全、方便及毒副作用低等特点。 相似文献
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13.
Sagar Dholariya Deepak N. Parchwani Ragini Singh Madhuri Radadiya C. D. S. Katoch 《Indian journal of clinical biochemistry : IJCB》2022,37(2):131
The coronavirus disease 2019 is a highly contagious viral infection caused by SARS-CoV-2 virus, member of coronaviridae family. It causes life threatening complications due to complexity and rapid onset course of the disease. Early identification of high-risk patients who require close monitoring and aggressive treatment remains challengeable till date. Novel biomarkers which help to identify high risk patients at the early stage is high priority. Objective of this review to find utility of P-SEP, sTREM-1 and suPAR for diagnosis, risk stratification and prognosis of SARS-CoV-2 infected cases. Soluble receptors like, P-SEP, sTREM-1 and suPAR have been involved in immune regulation in SARS-CoV-2 infection and elevate more in severe cases. A comprehensive research of databases like PubMed, EMBASE, CNKI and Web of Science was performed for relevant studies. A total of nine out of fifteen research literature in initial screening were included for this review. Interestingly all studies have reported high levels of P-SEP, sTREM-1 and suPAR in SARS-CoV-2 infected cases and the biomarkers positively correlated with severity of infection. This implies that P-SEP, sTREM-1 and suPAR can be implemented as surrogate marker in blood profile for early diagnosis, risk stratification and prognosis in SARS-CoV-2 for better management in Indian population at the current situation. 相似文献
14.
Phenotypic Heterogeneity of Asian Indian Inversion Deletions Gγ(Aγδβ)0 Breakpoint A and Breakpoint B
Sanjay Pandey Sweta Pandey Ravi Ranjan Rahasyamani Mishra Monica Sharma Renu Saxena 《Indian journal of clinical biochemistry : IJCB》2013,28(1):98-101
Asian Indian inversion deletion Gγ (Aγδβ)0-thalassemia is a rare entities characterized by high HbF. Due to interaction with various genetic factors, patients with Gγ (Aγδβ)0-thalassemia showed clinical variability. Here we are presenting the phenotypic expression of Gγ(Aγδβ)0 thalassemia under influence of various co-inherited factors. Patient with α-globin gene deletion had mild phenotype than the patient with β-globin mutations. Patient with alpha gene deletion were presenting clinical character like thalassemia intermedia while Gγ (Aγδβ)0-thalassemia patients with co- presence of beta thalssemia mutation clinically behaved like thalassemia major. 相似文献
15.
16.
《普罗米修斯》2012,30(2):272-274
Great progress in the field of recombinant DNA technology has masked concern about the safety of the procedures involved. It seems that those carrying out oncogene research using these techniques may be exposed to considerable danger of contracting cancer. A thorough program to assess the risks involved is required to replace the complacency of ignorance which now exist, and it is required before rather than after damage is done. 相似文献
17.
18.
M.T. Gaudin 《Research Policy》1976,5(2):106-114
A survey of French public opinion towards innovation and technical progress was undertaken in March 1975. It creates an artificial way of life, threatening the life of the next generation, and turning men into robots. Artificial needs are created to sell new consumer products. But innovations transforming production methods are beneficial to both management and labour. Technical progress will contribute in future to improving production methods, to raising levels of income, to helping developing countries, and to solving the energy crisis; it will not contribute to the reduction of unemployment, the reduction of risks of war, increasing harmony with nature, or giving a poetic sense to life.Major priorities for innovation in the next few years should be the reduction of waste of all sorts, the exploitation of new energy sources, the improvement of working conditions, and the protection of nature. These severe judgements by French public opinion of the industrial way of life and of technical progress have been presented to industrialists, who questioned the validity and the utility of this sort of inquiry. 相似文献
19.
浙江地区中华鳖腐皮病的病原与药敏研究 总被引：3，自引：0，他引：3
中华鳖腐皮病是危害中华鳖最严重的常见病之一,其发病率较高,四季均可流行,给养鳖业生产造成较大经济损失。本研究从浙江地区患病个体的颈部病灶、足部病灶、肝脏及肠壁中共分离到6支菌株。经人工感染毒力测试。鉴定病原为嗜水气单胞菌Aeromonas hydrophila与温和气单胞菌Aeromonas sobria。药敏试验结果表明,这两种致病菌均对新霉素、卡那霉素、氯霉素、氟嗪酸、庆大霉素、红霉素等抗生素高度敏感。而对15种中草药仅高敏于五倍子。 相似文献
20.
Santosh Shinde Pawan Kumar Kaushala Mishra Neela Patil 《Indian journal of clinical biochemistry : IJCB》2006,21(2):156-160
Mitochondrial diseases are a heterogeneous group of disorders in which a primary mitochondrial dysfunction is proven by morphological,
biochemical, and genetic examinations. The mitral valve has important function in the regulation of blood flow from one chamber
to another. Often, the mitral valve becomes abnormal with age, in Rheumatic fever or it is abnormal from birth (Congenital)
or it can be destroyed by infection i.e. bacterial endocarditis and needs replacement. Myocardial function depends on energy
produced by mitochondria and in any of these disease conditions, mitochondrial functions and enzyme activities may be impaired.
With this in view, we analyzed the relationship between preoperative clinical conditions (as per New York heart Association)
and extent of mitochondrial enzyme activities in damaged Human mitral valve in two types of heart disease such as Rheumatic
Heart Disease (RHD) and Bacterial Endocarditis (BE). Thirty nine Patients undergoing cardiopulmonary bypass (CPB) for routine
valvular heart surgery were included in the study. Controls included 11 normal porcine mitral valve samples without any evidence
of heart disease. Mitochondrial enzymes like cytochrome oxidase (COX), succinate dehydrogenase (SDH), malate dehydrogenase
(MDH), citrate synthase (CS) and ATPase were determined. Mitochondrial COX, SDH, CS and Total ATPase activities were significantly
decreased in disease condition like BE and RHD when compared with control (P<0.001). On the other hand as per New York Heart
Association (NYHA) preoperative clinical classification, all the mitochondrial enzymes were significantly (p<0.05) impaired
in class IV as compared with NYHA class I, II and III. Present study shows that impairment in the mitochondrial enzymes activities
are more pronounced in bacterial endocarditis (BE). It also indicates that damage to mitochondrial enzymes are most pronounced
in NYHA class IV. 相似文献