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1.
以苯酚作为唯一碳源和能源的无机盐培养液对从绝缘材料厂排放的活性污泥进行驯化,从中分离筛选到1株苯酚降解菌,编号为JY04.采用正交实验设计方法优化了该菌株固定化条件,并且考察了该固定化细胞降解苯酚的最适条件.结果表明:在固定化条件为海藻酸钠(SA)浓度为5%,湿菌重/包埋剂为6/30,CaCl2浓度为3%时,固定化时间为8 h,降解率可达82.6%,并具有较好的机械强度.确定其对苯酚降解最适条件为pH 7.0,苯酚浓度为400 mg/L,温度32℃,接种量为25 g,培养时间42 h,其苯酚的降解率可达97.3%.该菌株固定化细胞的降解苯酚能力和耐受苯酚能力均大于游离细胞.固定化菌株对苯酚有较强的降解和耐受能力,是处理苯酚废水的良好菌种资源. 相似文献
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从生活污泥中培育出高效降酚菌,并挂膜培育了高效酚降解生物膜;驯化、培养、分离出两株对酚具有较高降解能力的微生物菌株,研究了其降解苯酚的性能和环境因素对降酚效果的影响。结果表明:在pH=5~7及25~35℃的环境下,含酚污水中酚降解率可达80%以上。 相似文献
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从皖北不同地区采集土壤样品,利用宿主结瘤法获得大豆根瘤,并采用平板划线法分离不同地区大豆根瘤菌株,共31株,对其进行染料及化学药物抗性、耐酸碱性、生长温度范围、耐盐性、抗生素抗性等性状的研究。结果表明,从31个菌株中筛选出总体抗性水平最为突出的菌株AWCS 13-4,其表现抗染料及化学药物能力最强;在pH4-pH12条件下生长良好,具有较广的酸碱度适应范围;在50℃条件下,仍可以缓慢生长;在6.0%NaCl处理下仍能生长,显示出很强的耐盐能力;并且在供试5种抗生素的不同浓度条件下均生长良好,表现出极强耐抗生素的能力。 相似文献
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《绵阳师范学院学报》2017,(5):50-54
为了对筛选得到的1株纤维素分解菌进行鉴定,并研究其对废弃秸秆资源的降解效果.本文通过羧甲基纤维素钠(CMC)固体培养初筛和复筛,从土壤中获得了1株酶活力较高的菌株,并对其进行不同碳源酶活力的初步研究.利用PCR法,结合18S rDNA的序列分析鉴定该菌株为产黄青霉菌,将该菌株接种到不同碳源的发酵培养基中,30℃培养48 h后测定酶活,发现该菌株不到12 h对滤纸完全崩解;15 d内对小麦秸秆分解迅速.同时测得羧甲基纤维素酶活力、滤纸糖化力和小麦秸秆纤维素分解能力分别为:4.653 U/m L、5.445 U/m L和6.876 U/m L. 相似文献
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从受石油产品污染土壤中筛选出5株石油降解菌,并通过生理生化实验对其进行了初步鉴定。石油降解能力的测定结果表明,其中KF-2、KF-4两株菌均为微球菌属Microrococcussp．）,石油降解能力较强,其单菌落接种20天后石油降解率分别可以达到76．21％、74．82％。在对这两株菌的降解特征的进一步探究中发现：KF-2菌株在NH4NO3作为氮源的条件下石油降解率较高;而KF—4在（NH4）2SO4作为氮源的条件下石油降解率较高。环境pH为7时,KF-2、KF-4的石油降解率都最高。混合菌的试验表明,KF-2、KF-4混合菌的降解效果明显好于各单菌株接种降解效果。 相似文献
6.
《洛阳师范学院学报》2016,(2):47-50
从环境中筛选、驯化出一株高效降解油脂的酵母菌X3,然后对影响该酵母菌降解能力的几个因素进行了研究.结果表明,初始p H为3~4,初始含油量为600mg/L,培养温度为25℃,半静置条件下,该菌株对油脂的降解能力最强,而且对底物的选择性不严格. 相似文献
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目的：分离筛选出高效降解毒死蜱菌株,明确其生物学分类地位,并优化其降解条件.方法：采取平板划线和摇瓶复筛法从农药厂土壤样品中分离得到高效降解毒死蜱菌株,并通过16S rRNA序列测定和同源性分析对其进行鉴定;运用HPLC法检测降解菌株对毒死蜱的降解效果,研究其在不同培养基、接种量、温度及pH条件下的降解能力.结果：从农药厂土壤样品中筛选得到一株能够以毒死蜱为唯一碳源生长的高效降解菌株OP7,经16S rRNA基因序列聚类分析,将其初步鉴定为粪产碱杆菌(Alcaligenes faecalis).进一步的降解条件优化结果显示,菌株OP7降解毒死蜱的最佳培养基是10%LB、最适接种量为3%(v/v)、最适温度为35℃、最适初始pH为9.0.结论：本研究结果表明菌株OP7有望成为新的菌种资源并应用于环境中毒死蜱污染物的生物修复. 相似文献
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优良菌对原油降解性能的试验研究 总被引：1，自引：0，他引：1
以原油为唯一碳源,从长期被石油污染土壤的浸泡液中分离、筛选出二株降解原油的优良菌SY4和SY6,初步鉴定为:SY4和SY6为芽孢杆菌属;通过降解性能实验的研究得出:2株菌对原油都有较强的降解能力,在摇床实验中,单一菌株在5天后的原油降解率都高于60%.摇床实验和静态曝气实验都得出:SY6的降解能力比SY4强.实验研究结果为其实际应用提供了一定的生物基础. 相似文献
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通过对钾细菌UV诱变处理,获得了两株正变株,其解钾能力分别为7.05mg/m1(K2O)和10.25mg/ml(K2O),较出发菌株提高1282.35%、1909.80%.并且通过酸碱、温度驯化,得到一株正抗性变异株,其解磷解钾能力分别是73.mg/ml(P2O5),4.41mg/ml(K2O),较出发菌株分别提高35.97%,25.53%. 相似文献
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目的:明确一株蜡状芽孢杆菌(Bacillus cereus HY-4)对毒死蜱的降解特性。方法:采用UV全波长扫描、灵敏度和添加回收率测定等建立了毒死蜱的定量检测方法,以及通过定量添加毒死蜱和降解菌的方法研究了菌株HY-4对毒死蜱的降解特性。结果:毒死蜱的最佳测定波长为292.0 nm,经验证该检测方法的线性相关性、灵敏度、准确度和变异系数均符合菌株降解毒死蜱残留检测的要求。降解菌HY-4对50.0mg/L毒死蜱的降解率在摇培36 h时达到最高,适量添加葡萄糖能够促进降解菌的生长和毒死蜱的降解,葡萄糖添加量为0.5%时对50.0 mg/L毒死蜱的降解率达最高为88.0%;毒死蜱初始浓度对降解率也有较大影响,毒死蜱初始浓度为50.0 mg/L时降解率达最高为89.6%,然而当毒死蜱初始浓度继续升高时,菌株对毒死蜱的降解率下降,毒死蜱初始浓度为200.0 mg/L时,降解率下降至59.3%。结论:降解菌HY-4对毒死蜱的降解能力较强,在毒死蜱污染水体等的微生物降解方面应用价值较大。 相似文献
11.
罗艳萍 《读与写:教育教学刊》2008,5(6)
The communication of people partially is the communication of cultures. Culture has a direct effect on international commercial activities in all aspects. Different conceptions about time, space, equality, law and the like, lead people to deal with things in different ways. So to know cultures of the counterpart is to facil-itate our enterprises so as to have a smooth and successful communication in commercial activity. 相似文献
12.
Richard Denner 《海外英语》2007,(4):14-15
Rosco and I wait for the fishermen to return.I sit at a wooden bench near the store at Mt.Baker Resort and watch the clouds change shape. Rosco has my belt around his neck and an eight foot tow chain hooked to a tree. Dogs must be on a leash. Ducks and rabbits are loose. 相似文献
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Given a graph G,a subgraph C is called a clique of G if C is a complete subgraph of G maximal under inclusion and |C|≥2. A clique-transversal set S of G is a set of vertices of G such that S meets all cliques of G. The clique-transversal number, denoted as TC (G), is the minimum cardinality of a clique-transversal set in G. The clique-graph of G, denoted as K (G), is the graph obtained by taking the cliques of G as vertices, and two vertices are adjacent if and only if the corresponding cliques in G have nonempty intersection. Let F be a class of graphs G such that F={G|K(G) is a tree}. In this paper the graphs in F having independent clique-transversal sets are shown and thus TC (G)/|G|≤1/2 for all G ∈ F. 相似文献
14.
邱崇丙 《唐山师范学院学报》2014,(1):21-23
汉字的六书,《说文》对“转注”一类,语焉不详。后世学者提及转注,也仅限于许慎所举出的例字。《汉字例说》一文,作者从转注的角度综合考虑,对部分现代常用字作了分析,跟传统的解释有所不同。希望能抛砖引玉,互相切磋,以推进学术研究。 相似文献
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《海外英语》2007,(5):10-11
Many college freshmen arrive woefully unprepared to do college work, and as disadvantaged populations continue to grow, the share of the American work force that has made it through college is expected to plummet. Many experts blame that educational failure not just on high schools but also on colleges. School & College, a special report by The Chronicle, looks at efforts to fix the system. What reforms would better prepare students for college? What should schools and colleges be doing differently? How should state and federal officials help? 相似文献
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陈美曦 《山东教育学院学报》2008,23(2):103-107
Different nations have different social cultures, and the social culture of every nation is developing with the development of the nation, language is greatly influenced by social culture, which enables language to possess national and historical characteristics. Word is the smallest, independent, meaningful linguistic unit of language. It can well reflect the national and historical characteristics. From synchronic aspect, the social and cultural influence on word meaning is mainly embodied in conceptual and associative meanings of word. The incorrespondence of word meaning is a phenomenon that exists in both conceptual and associative meanings of word among different languages. From diachronic aspect, the development of social culture causes some changes of word meaning to some extent. Therefore, social culture plays a key role in vocabulary learning and mastery of a language. 相似文献
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Rachel Zupek 《海外英语》2007,(11):40-41
我们绝大部分人都同意一件事情——我们喜欢金钱。但是你对金钱的喜欢之情足够到让你创立一份事业么?.财富通常被人忽视,主要在于人们不能真正了 相似文献
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《海外英语》2007,(4):36
There are numbers of crossroads on our long and unpredictable life journey where we totally have no idea about which direction to choose. No matter what our decision is, we should not turn back, but face the music and go ahead instead. I am this kind of girl who always does try without regretting, one example is how I dealt with my love. 相似文献