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目的:研究巨噬细胞移动抑制因子(MIF)是否通过PI3K/AKT通路对肺腺癌A549细胞的增殖、凋亡、迁移产生影响。方法:免疫组织化学技术检测MIF抗体在肺腺癌癌组织和癌旁组织中的表达情况,DEME完全培养基培养肺腺癌A549细胞株作为对照组,加入不同浓度人重组巨噬细胞移动抑制因子(rh MIF)和PI3K/AKT特异性抑制剂LY294002预处理作为实验组,应用MTT(噻唑蓝比色法)观察A549细胞株的增殖情况,(2) FCM(流式细胞仪技术)检测A549细胞株凋亡情况(3) Western-Blot测定A549细胞株PI3K、p AKT及相关抗凋亡抗体p53、Bcl-2蛋白表达情况。结果:(1)免疫组化发现MIF在肺腺癌癌组织中高表达,且高表达率与癌旁组织有显著差异(2)经rh MIF刺激后PI3K、p AKT、p53、Bcl-2表达更明显,迁移更显著;(3) rh MIF对A549细胞株作用均呈浓度-时间依赖性,rh MIF在50 ng/ml 24 h对A549细胞增殖率最高,LY294002在25μmol/L 24 h对A549细胞抑制作用最显著。结论:MIF在肺腺癌中高表达,肺腺癌细胞中加入rh MIF,rh MIF刺激A549细胞株增殖、迁移,抑制凋亡,与LY294002共同作用下,A549细胞株增殖率和迁移力下降、凋亡率升高,表明MIF有可能通过PI3K/AKT通路对肺腺癌细胞的增殖、凋亡和迁移产生影响。 相似文献
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采集并检测北京市玉泉路地区2008年冬季和夏季大气颗粒物PM10和PM2.5中过渡金属元素的含量,测定A549细胞暴露于颗粒物后的细胞活力及活性氧(ROS)生成.用线性回归统计分析颗粒物中过渡金属元素含量与其细胞毒性的相关性.结果表明,冬季和夏季PM10和PM2.5均明显抑制A549细胞的细胞活力,导致ROS生成升高.颗粒物细胞毒性夏季大于冬季;除Cr、Ni外,各金属元素与颗粒物导致的细胞毒性正相关.冬季和夏季颗粒物中金属元素含量的差异是导致其细胞毒性不同的原因之一. 相似文献
3.
长期高血糖导致糖尿病患者的氧化应激,引起胰岛β细胞氧化损伤,但核转录因子Nrf2(NF-E2-related factor 2)抵抗胰岛β细胞氧化损伤的作用机制还不清楚.本研究用低浓度葡萄糖(LG,5.6 mmol/L)、LG+H2O2和高浓度葡萄糖(HG,27.6 mmol/L)分别处理小鼠胰岛NIT-1β细胞48 h,检测细胞内活性氧(ROS,reactive oxygen species)生成、胰岛素合成与分泌变化和Nrf2入核表达水平.研究发现,高糖诱导NIT-1β细胞的ROS生成,降低细胞合成与分泌胰岛素的水平,但Nrf2入核表达降低胰岛β细胞氧化应激.结果提示Nrf2入核表达可以抵抗高糖诱导的胰岛β细胞氧化损伤,改善细胞合成与分泌胰岛素的功能. 相似文献
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以海州湾常见优势藻种三角褐指藻(Phaeodactylum tricormutum)、海链藻(Thalassiosira weissflogii)为受试藻,考察了水环境中草甘膦致毒胁迫对二种藻生理特性的影响;同时以超氧化物歧化酶(SOD)活性、过氧化氢酶(CAT)活性及丙二醛(MDA)含量为指标,研究了这两种海洋微藻细胞内抗氧化系统对草甘膦胁迫的响应。低浓度的草甘膦(4.1 mg/L)对海链藻的生长促进作用显著,对三角褐指藻不显著;草甘膦可以降低这两种微藻叶绿素a的合成量,呈现质量浓度-剂量效应,且对三角褐指藻的毒性效应大于海链藻;草甘膦对这两种微藻的CAT活性、可溶性多糖含量和蛋白质含量均表现微弱抑制效应;低浓度草甘膦还可提高两种微藻的SOD活性和MDA含量。本文以提示农药污染对水环境的破坏,揭示海洋微藻类作为环境毒理学评价指标的潜在应用价值。 相似文献
6.
目的在开放式压力控制培养系统作用下体外培养大鼠视网膜神经细胞（retinal neurons,RNs）,观察米诺环素对其活性及凋亡的影响．并进—步探讨其对受损RNs保护的可能机制．方法采用出生0-3d的Sprague Dawlev（SD）大鼠视网膜神经细胞体外混合培养。制备RNs加压培养模型．通过细胞形态学观察,四唑盐（MTT）比色法测定细胞活力,吖啶橙／溴化乙锭（AO／EB）染色法检测细胞的凋亡率来观察米诺环素对上述损伤细胞的保护及治疗作用．以及应用免疫细胞化学染色法。观察诱导型一氧化氮台酶（iNOS}和半胱天冬酶-3,（caspase-3）表达的改变、结果 加压培养后,在倒置显微镜下RNs与对照组相比形态改变较咀.细胞活力降低,大量细胞发生凋亡（占53.93％）.而米诺环素治疗组（20μmol／L）细胞则形态改善．活力显著增高：凋亡数目减少（占17．29％）．差异具有显著性（P〈0.0051）。免疫细胞化学染色法示米诺环素治疗组细胞内NOS和caspase-3表达较加压损伤组减少。结论 一定剂量的米潜环素在体外可有效抑制蓝力。引起的大鼠视网膜神经细胞损伤及凋亡。抑制NOS和capase-3的表达可能是其潜在作用机制。 相似文献
7.
目的:研究胡桃醌诱导人乳腺癌MCF-7细胞凋亡及其形态学观察。方法:四甲基偶氮唑蓝(MTT)法检测胡桃醌对MCF-7细胞的增殖抑制作用;Hochest33258荧光染色法观察细胞凋亡形态;流式细胞术(FCM)检测细胞凋亡率。结果:胡桃醌对人乳腺癌MCF-7细胞的IC50为11.99μmol/L;胡桃醌作用24h后,细胞生长密度变疏,细胞皱缩,出现凋亡小体,且随着给药浓度升高,凋亡小体也逐渐增多;胡桃醌5、10、20μmol/L作用24h后MCF-7细胞凋亡率分别为6.35%、12.43%、28.55%。结论:胡桃醌对人乳腺癌MCF-7细胞具有增殖抑制作用。 相似文献
8.
叠氮钠(NaN3)诱变对雨生红球藻的生理效应 总被引：1，自引：0，他引：1
用浓度分别为0、0.5、1、1.5、2、2.5、3、3.5、4、4.5、5、6、7、8、9、10 mmol/L的叠氮钠（NaN3）处理雨生红球藻（Haematococcus pluvialis）,6 d后用细胞计数测定致死率。然后取浓度分别为0、1、2、3、4、5、6mmol/L的NaN3处理过的存活细胞,经数代培养后以相同起始浓度在适宜条件下培养10 d,然后测定生长指标。结果表明：浓度为1mmol/L的NaN3处理有利于促进藻细胞的生长,而其它浓度处理则对藻生长起抑制作用;但不同浓度处理都有利于促进虾青素的积累,其中又以1mmol/L的NaN3处理后的虾青素含量最高,之后随处理浓度的增加虾青素量反而下降;结果还发现一定浓度的NaN3处理有利于提高藻耐高温的能力,但对耐低温能力影响不大。 相似文献
9.
车前草总黄酮清除自由基及对小鼠氧化损伤的保护作用 总被引：1，自引：0，他引：1
目的研究车前草总黄酮体外清除自由基及对D-半乳糖诱导小鼠氧化损伤的保护作用。方法采用化学模拟体系研究了车前草总黄酮（total flavonoids from Plantago asiatica Linn.,TFPA）对DPPH自由基和ABTS＋自由基的清除能力及对脂质体过氧化的抑制作用。采用D-半乳糖建立小鼠氧化损伤模型,研究了不同剂量TFPA（100、200、500 mg/kg.bw）对小鼠体重、脏器指数及血清、肝脏中丙二醛（MDA）含量和谷胱甘肽过氧化物酶（GSH-Px）、超氧化物歧化酶（SOD）活性的影响。结果TFPA可有效清除DPPH自由基和ABTS＋自由基,抑制脂质体过氧化,其IC50分别为9.00 mg/L、107.93 mg/L和18.28 mg/L。TFPA可使氧化损伤小鼠体重增量、胸腺指数显著升高（p〈0.01）,使肝脏、肾脏和脾脏指数也出现一定程度的升高（p〈0.05）;TFPA可有效降低氧化损伤小鼠血清和肝脏中MDA含量,最大降幅分别为22%和14%;同时有效增高氧化损伤小鼠血清和肝脏中GSH-Px、SOD的活性,且具有量效关系。结论TFPA可显著增高氧化损伤小鼠体内抗氧化酶活性,改善脂质过氧化．从而明显保护D．半乳糖诱导的氧化损伤。 相似文献
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Rukmini M. S. Benedicta D'Souza Vivian D'Souza 《Indian journal of clinical biochemistry : IJCB》2004,19(2):114-118
Free radical mediated pathological processes may have a role in schizophrenia. Free radicals (oxy radicals, such as superoxide,
hydroxyl ions and nitric oxide) cause cell injury, when they are generated in excess or when the antioxidant defense is impaired.
Both these processes seem to be affected in schizophrenia. In this study we investigated erythrocyte superoxide dismutase
(SOD) and catalase (CAT) activities as antioxidant enzymes, malondialdehyde (MDA) as a sign of lipid peroxidation in schizophrenic
patients. Activities of superoxide dismutase, catalase and malondialdehyde were greater in patients compared with the control
group which may reflect increased oxidative stress in the brain tissue of schizophrenics. In the patient group erythrocyte
SOD and CAT activities were weakly negative correlated with MDA concentration. These data reveal that antioxidant defense
mechanisms might be impaired in schizophrenic patients. These findings also provide a theoretical basis for the development
of novel therapeutic strategies, such as antioxidant supplementation. 相似文献
12.
烟雾吸入伤早期氧化与抗氧化动态平衡变化的实验研究 总被引：1，自引：0，他引：1
采用大鼠烟雾吸入伤模型，动态观察了伤后血浆、支气管肺泡灌洗液（ＢＡＬＦ）和肺组织中超氧化物歧化酶（ＳＯＤ）活性、丙二醛（ＭＤＡ）和共轭二烯（ＣＤ）含量的变化，辅以动脉血气分析、肺水量测定、ＢＡＬＦ中白细胞分类计数和病理检查．结果发现，伤后动物出现急性呼吸衰竭和严重肺水肿，肺内大量炎细胞聚集浸润；血浆、ＢＡＬＦ及肺组织中ＳＯＤ活性明显降低，ＣＤ和ＭＤＡ含量显著增加，ＭＤＡ／ＳＯＤ比值亦明显增高，且与ＢＡＬＦ中中性粒细胞数增加显著相关，提示氧化与抗氧化平衡失调可能在急性烟雾吸入性肺损伤的发生发展中起重要作用 相似文献
13.
Curcumin is a natural anti-inflammatory and antioxidant with several potential health benefits. Although it has been examined in several metals toxicity studies, but its role in the protection against copper toxicity has not been investigated. In this study; the detoxification and antioxidant effect of curcumin were examined to determine its prophylactic/therapeutic role experimentally in rats. Forty albino rats were divided into five groups; control, CuSO4 (4 mg/kg body weight), curcumin (80 mg/kg body weight), curcumin post-treatment (CuSO4 for 15 days followed by curcumin for the next 15 days) and curcumin co-treatment (CuSO4 plus curcumin for 30 days). All rats were treated orally by stomach tube for 30 days/once a day. Changes were observed in hepatic marker enzymes such as: aspartate aminotransferase (AST), alanine transaminase-(ALT), alkaline phosphatase (ALP) and gamma-glutamyltransferase (GGT), besides the serum total protein, urea and creatinine. Concentration of liver and kidney antioxidants such as: catalase (CAT), superoxide dismutase (SOD), reduced glutathione-(GSH) and malondialdehyde (MDA) were measured. An increased in the activities of liver marker enzymes, urea, creatinine and the MDA contents were detected after exposure to CuSO4. Meanwhile, the activities of serum total protein, hepatic and renal antioxidants were decreased. Changes in all biochemical parameters were alleviated by the post-treatment and co-treatment of curcumin. Our finding suggests that the curcumin showed protective effects on CuSO4-induced hepatotoxicity and nephrotoxicity. 相似文献
14.
Soma Gupta K. K. Singh V. J. Vyas V. N. Chaturvedi M. V. R. Reddy B. C. Harinath 《Indian journal of clinical biochemistry : IJCB》2000,15(1):52-55
Oxidative stress was studied by estimating plasma levels of malondialdehyde (MDA), beta carotene, vitamin E and erythrocytic
superoxide dismutase(E-SOD) activity in 50 cases of carcinoma of upper digestive tract which included carcinoma of oral cavity,
pharynx and oesophagus. While plasma MDA level was found to be increased (3.5±1.0 nmole/ml), a significant decrease in beta
carotene (81.2±14.5mg%), vitamin E (8.5±1.1 mg/L) level and E-SOD activity (657.0±80.6 U/G Hb) were observed in carcinoma
of upper digestive tract. Patients were treated with radiotherapy which itself was toxic enough and produced its deleterious
effects by generation of reactive oxygen species (ROS). As antioxdiants can detoxify ROS, beneficial effect if any, of antioxidant
administration during radiotherapy was studied in two groups of patients, group A (n=5, supplemented with antioxidants) and
group B (n=5, without antioxidant supplementation). Plasma MDA level was found to be elevated in both the groups but the increase
in group B was significant, compared to pretreatment level. Further, body weight was found to be significantly decreased in
group B patients, which was maintained in group A patients. Moreover, group A patients showed significant elevation in beta
carotene concentration, thus showing beneficial effect of administration of antioxidants during radiotherapy without disturbing
the desirable therapeutic effect of radiotherapy. 相似文献
15.
Caffeic acid is a well-known phenolic compound widely present in plant kingdom. The aim of this study was to investigate the
possible protective effect of caffeic acid (CA) against oxytetracycline (OXT) induced hepatotoxicity in male Albino Wistar
rats. A total of 30 rats weighing 150–170 g were randomly divided into five groups of six rats in each group. Oral administration
of OXT (200 mg/kg body weight/day) for 15 days produced hepatic damage as manifested by a significant increase in serum hepatic
markers namely aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase
(LDH), bilirubin and increased plasma and hepatic lipid peroxidation indices (TBARS and hydroperoxide). The present finding
shows that the levels of enzymatic antioxidants namely superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase
(GPx) were significantly decreased in OXT intoxicated rats. Upon oral administration of caffeic acid (40 mg/kg body weight/day)
there were decreased hepatic marker activities, bilirubin and lipid peroxidation and increased enzymatic antioxidants in OXT + Caffeic
acid group compared to Normal + OXT group(P < 0.05). Our study suggests that caffeic acid has antioxidant property and hepatoprotective ability against OXT induced toxicity. 相似文献
16.
Ranjana Singh Rajesh K. Singh Anil K. Tripathi Nikhil Gupta Ajai Kumar Anil K. Singh Abbas A. Mahdi Rajendra Prasad Raj K. Singh 《Indian journal of clinical biochemistry : IJCB》2004,19(1):14-20
The circadian periodicity of plasma lipid peroxide levels and activities of superoxide dismutase (SOD), catalase (CAT) and
glutathione peroxidase (GPx) were studied in 50 clinically, bacteriologically and radiologically proven fresh cases of pulmonary
tuberculosis (age: 21–45 years) and 60 age-matched healthy volunteers with diurnal activity from 06∶00 to about 22∶00 and
nocturnal rest. A marked circadian variation in plasma lipid peroxide level was recorded in healthy subjects and pulmonary
tuberculosis patients with significant amplitude and acrophase around 16∶21 and 17∶12 respectively. The acrophase tended to
be delayed in tuberculosis patients. Furthermore, a statistically significant circadian rhythm was found in SOD, CAT and GPx
activities in normal volunteers and pulmonary tuberculopsis patients. SOD and CAT enzyme activity was noted to be maximum
at 06∶00 and minimum at 00∶00 in tuberculosis patients. The circadian acrophase for GPx activity was recorded at 16∶15 in
normals and around 22∶45 in patients. Moreover, the activity was found to be decreased at all sampling hours during 24-hours
sleep-awake period in patients in comparison to healthy counterparts. The MESOR and circadian amplitude also decreased markedly.
The decreased activity of measured antioxidant enzymes in pulmonary tuberculosis patients could probably be associated with
oxidative stress and/or decreased anti-oxidant defensive mechanism in such patients. 相似文献
17.
Servin Yeşil Günal Bilal Üstündağ Ali İhsan Günal 《Indian journal of clinical biochemistry : IJCB》2013,28(4):390-395
The aim of this study is to evaluate the oxidative stress in predialysis, hemodialysis (HD) and peritoneal dialysis patients and to test the effects of antihypertensive drugs and volume control on oxidative stress parameters. The study was composed of five groups as follows: control group (n = 30), predialysis group (n = 30), peritoneal dialysis group (n = 30), hemodialysis group, (normotensive with strict volume control, n = 30), hemodialysis group (normotensive with medication, n = 30). Plasma malondialdehyde (MDA), erythrocyte superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSHPx) and routine biochemical parameters were studied in all patients. Hemodialysis patients with strict volume control (HDvc) had lower levels of MDA than other patient groups (p < 0.001), and CAT, SOD values had highest level other patient groups (p < 0.001). The treatment of hypertension with strict volume control in chronic renal failure patients causes less damage to the antioxidant capacity. 相似文献
18.
印度芥菜BjPCS1基因的表达提高烟草对重金属的抗性 总被引：4，自引：0，他引：4
在植物体内植物络合素(PC)可与重金属螯合,并进一步转运至液泡贮存,使细胞质的重金属浓度降低,从而达到解毒效果。PC不是基因的直接翻译产物,而是以谷胱甘肽(GSH) 为底物由植物络合素合酶催化而成。将来源于重金属超富集植物印度芥菜(Brassica juncea)的植物络合素合酶基因BjPCS1转入烟草,PCR和Northern结果表明,该基因已经整合到烟草基因组中,并在转录水平上表达。在3种重金属(200mmol/L CdCl2、400mmol/L ZnCl2、200mmo/L NiCl2)胁迫条件下,转基因烟草植株的脯氨酸含量、可溶性糖含量、丙二醛含量、相对电导率和叶绿素含量等指标均优于未转化的对照植株,表明转化BjPCS1基因提高了烟草对3种重金属的抗性,其中转基因烟草抗Cd能力最强,并且转基因烟草的T1代种子在Cd处理的情况下萌发状况明显优于对照。这些结果说明BjPCS1基因在利用基因工程改良植物抗重金属能力和净化环境污染方面,具有良好的应用前景。 相似文献
19.
Krishna Mohan Surapneni V. S. Chandrasada Gopan 《Indian journal of clinical biochemistry : IJCB》2008,23(1):41-44
The changes in the erythrocyte lipid peroxidation products (MDA), levels of glutathione (GSH), ascorbic acid and plasma vitamin
E (non enzymatic antioxidant parameters) and activities of antioxidant enzymes superoxide dismutase (SOD), glutathione peroxidase
(GPX), catalase in erythrocytes and plasma glutathione - S - transferase (GST) activity were estimated in patients with rheumatoid
arthritis. This work was undertaken to assess oxidative stress and anti oxidant status in patients with rheumatoid arthritis.
It was observed that there was a significant increase in erythrocyte MDA levels, activities of SOD, GPX, plasma GST and a significant decrease in erythrocyte GSH, ascorbic acid, plasma vitamin E levels and catalse activity in
patients with rheumatoid arthritis when compared to controls. The results of our study suggests higher oxygen free radical
production, evidenced by increased MDA and decreased GSH, ascorbic acid, vitamin E and Catalase activity, support to the oxidative
stress in rheumatoid arthritis. The increased activities of antioxidant enzymes may be a compensatory regulation in response
to increased oxidative stress. 相似文献