共查询到20条相似文献，搜索用时 421 毫秒
1.
采用Trivelli等报道的Bio-Rex70阳离子交换树脂微柱层析法和Roger等报道的果糖胺法(两法均作了适当修改),分别对73例糖尿病患者及61例正常人进行了HbA_(1c)、HbA_1及糖基化血浆蛋白(Gpp)的测定.结果显示患者与正常人之间HbA_(1c)、HbA_1、Gpp均值有非常显著性差别.患者空腹血糖均值与HbA_(1c)、HbA_1、Gpp均值之间均有极显著的相关性.患者HbA_(1c)与HbA_1之间相关系数有极显著意义.证明HbA_(1c)、HbA_1与Gpp均可作为糖尿病控制较准确的客观指标.作者在提高微柱法分离度的关键问题上作了改进,找出了恰当的洗脱液Ⅰ与Ⅱ的Na~+浓度,分别为0.020mol/L与0.070mol/L.分离HbA_(1a+b)与HbA_(1c)结果尚称满意,柱间CV分别为3.1％与2.2％.在降低洗脱液Ⅰ、Ⅱ、Ⅲ中剧毒物质氰化钾的浓度问题上也作了研究,找出了较文献报道低2.5倍的氰化钾浓度,即由0.010mol/L降低到0.004mol/L.在果糖胺法中,采用5min与15min两次比色,用10％冰醋酸终止反应,提高了此法的精密度与重复性,批内CV为2.3％,批间CV为2.7％. 相似文献
2.
建立微芯片电泳-电化学检测技术分析细胞内还原型(GSH)和氧化型(GSSG)谷胱甘肽的新方法.分别考察缓冲液pH值、缓冲液浓度、SDS浓度、分离电压、进样时间、检测电位等因素对GSH、GSSG分离检测的影响.在最优实验条件下,在3 min内实现GSH和GSSG的有效分离和检测.GSH和GSSG的线性范围分别为5.0~200.0μmol/L和2.0~100.0μmol/L(R2>0.99),最低检测限(S/N≥3)分别为4.87μmol/L和1.98μmol/L.最终将所建立的方法应用于细胞样品中2种物质含量的测定,结果令人满意. 相似文献
3.
目的:建立凝胶色谱法测定头孢哇林钠的聚合物的方法.方法:采用葡聚糖凝胶Sephadex G-10柱(16mm×33cm),流动相:A:0.01mol/L磷酸盐缓冲液(pH7.0),B:0.01%十二烷基硫酸钠溶液.流速为1.0mol/min;检测波长为254nm,进样量为200uL.结果:头孢唑林钠在6.696～29.54mg/mL范围内,浓度与聚合物的峰面积呈良好线性关系(r=0.9986),可精确对高分子杂质峰进行定量.结论:该方法简便,准确,重现性好. 相似文献
4.
本实验首先在MS基本培养基上培养盐地碱蓬(SuaedaSalsa(L.)Pall.)的无菌苗,然后用上述无菌苗茎段为外植体在MS1培养基上诱导培养,初始愈伤组织为深黄色、非松脆型,愈伤组织诱导频率为95.64%以上.在继代培养基MS2上继代数月后得到了生长旺盛、疏松、呈乳白色的愈伤组织,用不同组成浓度的酶解液酶解松散型愈伤组织,进一步分离、提纯制备出具有活力的原生质体.以愈伤组织为材料制备原生质体的最适酶解液浓度为10g/L纤维素酶 5g/L离析酶 0.6mol/L甘露醇 0.05mol/L CaCl2.2H2O 0.1%MES.最适酶解时间为12-16h. 相似文献
5.
建立了同时测定氧化槐果碱、槐定碱和苦参碱的HPLC(高效液相色谱)方法.用VP-ODS(维普C18柱)150mm×4.6 mm,5 μm色谱柱,柱温40℃,流动相为0.01mol/L pH 8.5 磷酸盐缓冲溶液-甲醇(40:60),流速1.0 mL/min,紫外检测器波长215 nm.结果表明,3种生物碱在确定的色谱条件下得到很好的分离,且在所选质量浓度范围20~200 mg/L内线性关系良好,回收率范围97.2%~102%. 相似文献
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目的:建立双氯芬酸钠滴眼液中苯扎氯铵的含量测定方法。方法:高效液相色谱法,HYPERSIL BDS(250mm×4.6mm,5μm)色谱柱,0.02mol/L庚烷磺酸钠溶液(含0.1%三乙胺,用磷酸调至pH3.45±0.1)——乙腈(35:65)为流动相,检测波长为210nm,柱温40℃。结果:苯扎氯铵在19.52～214.72ug/ml浓度范围内进样量与峰面积线性关系良好,平均回收率为102.60%,RSD值为0.52%。结论:本方法简便、准确,可有效测定双氯芬酸钠滴眼液中苯扎氯铵的含量。 相似文献
9.
张凯 《内蒙古科技与经济》2005,(1):97-98
本实验探讨了电渗析器阳膜对不同阳离子透过量的差异。阳离子选用亚铁离子和铝离子．检测方法采用邻菲罗啉和8—羟基喹啉分光光度法。得出：在初始离子浓度为5mol／l、去除率为60％、温度为30℃、pH值为5的运行条件下,出水中Fe^2 和Al^3 的摩尔浓度差最大,为1．16mol／l。离子浓度为主要影响因素,对铝离子更具有通透性。 相似文献
10.
研究了改性黏土用量、溶液温度、铬离子Ce(Ⅵ)浓度.pH值和吸附时间对改性黏土吸附废水中Cr(Ⅵ)能力的影响.实验结果表明,改性黏土对废水中的Cr(Ⅵ)具有较好的去除效果.其最佳吸附条件为:改性黏土用量30g/L,温度30℃,pH=3,Cr(Ⅵ)质量浓度低于50mg/L,吸附时间30min. 相似文献
11.
Measurement of plasma hemoglobin is useful in variety of clinical conditions. In the present study we have developed a kinetic
method to estimate plasma haemoglobin by using o-tolidine. This method is sensitive, rapid, economical, simple and less influenced
by interfering substances. It measures plasma haemoglobin in the range of 6 to 400 mg/L (normal range < 50 mg/L) in less than
two minutes and can be easily automated. 相似文献
12.
Bratati Singh Debasis Debadatta Behera Neelam Mehta Seema Das 《Indian journal of clinical biochemistry : IJCB》2014,29(4):517-519
Glycosylated hemoglobin (HbA1c) determination is a powerful means for assessing the evaluation and management of patients with diabetes mellitus. Hemoglobin (Hb) variants and chemically modified derivatives of Hb can affect the accuracy of measurement of HbA1c done by various analytical methods. We report a patient with a rare variant of Hb (Hb Hope) that caused an abnormally high value of HBA1c when assayed using immunoturbidimetric assay (“Tina-quant” 2nd generation assay) and also elucidate the nature of the variant. 相似文献
13.
壳聚糖固定化血红蛋白氧载体的制备和性能研究 总被引：1，自引：0，他引：1
以固定化血红蛋白为核心的氧载体研究，有望为航空航天、水下作业以及好氧生物发酵工业等领域提供有效的供氧体系。以猪血红蛋白（pHb）和壳聚糖微粒为氧载体制备的基本原料，采用戊二醛活化将pHb共价固定在壳聚糖微粒上，构成pHb氧载体。研究了活化条件与载体表面醛基形成量及蛋白固定量之间的相互关系，对固定化反应进行了优化。在5％戊二醛浓度下活化反应8h，获得pHb最大固定量约800mg/g，即每克截体固定800mg pHb。从pHb氧载体的氧释放曲线可知，氧释放效率可达40％左右。该氧载体的结合稳定性较好。 相似文献
14.
Glycated hemoglobin levels in hemolysate of normal and diabetic patients were determined by the 2,6-dimethylphenol:57.5% sulphuric
acid conventional method and the values were 0.39±025 and 0.69±0.21 moles of hydroxymethylfurfural(HMF)/mole of globin, respectively.
The mean increase in glycated hemoglobin values in diabetics (1.8fold) was highly significant (p<0.001). A good correlation
(r=0.95) was found between the glycated hemoglobin values obtained by this method and the phenol:sulphuric acid method. The
values obtained by former method were about 1.2–1.4 times the values by the phenol:sulphuric acid method. This study indicates
that conventional 2,6-dimethylphenol: 57.5% sulphuric acid method is more sensitive for the estimation of glycated hemoglobin
than any other method based on the same principle. It is less time consuming, reliable and hence can be employed for the routine
laboratory estimation of glycated hemoglobin for the assessment of glycemic control. 相似文献
15.
?erif Ercan Mustafa ?al??kan Erhan Koptur 《Biochemia medica : ?asopis Hrvatskoga dru?tva medicinskih biokemi?ara / HDMB》2014,24(3):391-395
Introduction:
There are a number of pre-analytical and analytical factors, which cause false results in the complete blood count. The present case identifies cold agglutinins as the cause for the mismatch between hematocrit and hemoglobin values.Materials and methods:
70-year old female patient had a history of cerebrovascular diseases and rheumatoid arthritis. During routine laboratory examination, the patient had normal leukocyte and platelet counts; however, the hemoglobin (Hb: 105 g/L) and hematocrit (HCT: 0.214 L/L) results were discordant. Hemolysis, lipemia and cold agglutinin were evaluated as possible reasons for the mismatch between hematocrit and hemoglobin values.Results:
First blood sample was slightly hemolysed. Redrawn sample without hemolysis or lipemia was analyzed but the mismatch became even more distinct (Hb: 104 g/L and HCT: 0.08 L/L). In this sample, the titration of the cold agglutinin was determined and found to be positive at 1:64 dilution ratios. After an incubation of the sample at 37°C for 2 hours, reversibility of agglutination was observed.Conclusion:
We conclude that cold agglutinins may interfere with the analysis of erythrocyte and erythrocyte-related parameters (HCT, MCV, MCH and MCHC); however, Hb, leukocyte and platelet counts are not affected. 相似文献16.
Aim is to study the antidiabetic effect of a compound GII purified earlier from the water extract of fenugreek (Trigonella foenum graecum) seeds by Murthy and his colleagues (patented in India and USA) in diabetic rabbits. Diabetes was induced in rabbits by injecting
80 mg/kg bw of alloxan intravenously into rabiits. Rabbits were subdivided into subdiabetic [fasting blood sugar (FBG) up
to 120 mg/dl with abnormal glucose tolerance in glucose tolerance test (GTT)], moderately diabetic (FBG below 250 mg/dl) and
severely diabetic (FBG above 250 mg/dl). Blood glucose and glycosylated hemoglobin (HbA1C) were estimated by procedures in
the kits of Stangen Immunodiagnostics, Mumbai using, respectively, glucose oxidase method and absorbance at 415 nm. Serum
insulin was estimated by the ELISA method as described in the kit of Boehringer Mannheim Immunodiagnostics, Mumbai, India.
GII was found to improve blood glucose utilization in GTT and reduced FBG and HbA1C. In the present communication detailed
studies were carried out with GII in the subdiabetic, moderately diabetic and severely diabetic rabbits. GII at a dose of
50 mg/kg bw per day brought down the elevated FBG levels in the untreated subdiabetic (FBG 96.6 ± 7 mg/dl), moderately diabetic
(150.1 ± 14 mg/dl) and severely diabetic rabbits (427 ± 46 mg/dl) to normal in 12, 15 and 28 days of treatment. It improved
serum HbA1C and insulin levels also in these rabbits. Intermittent therapy once a week for 6 weeks with GII at the same dose
brought down the FBG values to normal in the subdiabetic (FBG 96.0 ± 2 mg/dl) and in the moderately diabetic rabbits to 133.0 ± 12 mg/dl.
After stopping therapy of the subdiabetic and moderately diabetic rabbits whose FBG values came to normal after treatment
with GII 50 mg/kg bw, the values remained normal for 1 week and showed a tendency to increase only after 15 days. If these
animal studies are applicable to humans these results indicate that a diabetic person need not take GII daily when once the
FBG value comes to normal or near to normal. Patients might be able to take GII only when the FBG value shows tendency to
increase. So, intermittent therapy is possible with the potent product GII of the fenugreek seeds which is of a great advantage. 相似文献
17.
Maryam J. Jalali Madhavi S. Phadke 《Indian journal of clinical biochemistry : IJCB》2011,26(4):407-412
Conditions like hypertension, atherosclerosis and diabetes are known to be the result of endothelial dysfunction which could
begin early in the life of an individual. The markers of endothelial dysfunction studied in the present work are plasma hemoglobin,
serum high sensitivity C-reactive protein (hsCRP) and plasma nitrite and nitrate. We studied the onset of endothelial dysfunction
with increase in age as well as in disease condition like newly detected hypertensives and of hypertensive diabetic patients.
The mean plasma hemoglobin and hsCRP values were found to increase with age. hsCRP and plasma hemoglobin levels were significantly
higher in patient groups as compared to aged-matched controls. On the other hand nitrate and nitrite was significantly higher
in patients who suffered from both diabetes and hypertension together and nitrate values were significantly lower in patients
who suffered from hypertensive alone. Plasma hemoglobin and hsCRP are associated with endothelial dysfunction and increases
both with age as well as in disease condition. 相似文献
18.
Non enzymatic glycosylation of IgG and their urinary excretion in patients with diabetic nephropathy
Diabetic nephropathy is a major cause of end stage renal disease. Increased excretion of albumin has widely been recognized
as an early manifestation of diabetic nephropathy particularly in subjects with diabetes mellitus. However, certain other
proteins besides albumin may be excreted in high amount during early phase of diabetic nephropathy. The serum and urinary
IgG, Glycosylated hemoglobin, fructosamine and glycosylated IgG were evaluated in the present study. Thirty-two patients of
Type 2 Diabetes without any complications, thirty-one patients of Type 2 Diabetes with nephropathy, twenty-six patients of
non-diabetic nephropathy and forty normal healthy individuals were enrolled in this study. Subjects were grouped based on
their serum creatinine level. Serum IgG, glycosylation of IgG and urinary IgG excretion were increased significantly in diabetic
patients compared to healthy controls, which were further increased significantly in chronic renal failure patients with respect
to the clinical stage of nephropathy. A positive correlation was observed between glycosylation of IgG and IgG excretion (R2=0.5995, 0.7114 respectively) in diabetic patients without any complications and diabetic nephropathy patients only, suggesting
a significant role of IgG glycosylation in the vascular clearances of IgG during diabetic nephropathy. 相似文献
19.
Kritsada Singha Goonnapa Fucharoen Supan Fucharoen 《Biochemia medica : ?asopis Hrvatskoga dru?tva medicinskih biokemi?ara / HDMB》2014,24(1):167-174
Hemoglobin (Hb) Grey Lynn is a Hb variant caused by a substitution of Phe for Leu at position 91 of α1-globin chain, originally described in individual of unknown ethnic background. This article addresses the interaction of Hb Grey Lynn with a non-deletional α+-thalassemia found in Thailand, a hitherto un-described condition. The proband was adult Thai woman referred for investigation of mild anemia with Hb 90 g/L. Hb analyses using low pressure liquid chromatography raised a suspicion of abnormal Hb presence, which was failed to demonstrate by cellulose acetate electrophoresis and capillary electrophoresis. DNA sequencing identified a CTT (Leu) to TTT (Phe) mutation at codon 91 corresponding to the Hb Grey Lynn (Vientiane) [α91(FG3)Leu>Phe (α1) on α1-globin gene and a C deletion between codons 36 and 37 on α2-globin gene causing α+-thalassemia. As compared to those observed in a compound heterozygote for Hb Grey Lynn / α0-thalassemia reported previously, higher MCV (81.7 fL) and MCH (26.3 pg) values with a lower level of Hb Grey Lynn (19.7%) were observed in the proband. The normochromic normocytic anemia observed could be due to the interaction of Hb Grey Lynn with α+-thalassemia. The two mutations could be identified using PCR-RFLP and allele-specific PCR assays developed. 相似文献
20.
P. K. Nigam S. Sharma R. Sareen V. Paul A. Lal 《Indian journal of clinical biochemistry : IJCB》2006,21(2):72-75
This study was carried out to check the precision of HbA1c values on Bio-Rad Variant II in cases of a rare hemoglobin variant Q India. The study was carried out over a three month
period on samples collected for HbA1c estimation. Seven out of eleven patients showed variable results of HbA1c with a very high and unacceptable intraday mean coefficient of variation (CV) of 9.93%. We conclude, that the results of
HbA1c on Variant II can not be reported without adversely affecting HbA1c as a marker of long-term glycemic control in patients who have hemoglobin Q India. The HbA1c value of these patients needs to be assessed by a different instrument/method or the glycemic control be monitored by an
alternate test like serum Fructosamine. 相似文献