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1.
Lim CY  Lam YC 《Biomicrofluidics》2012,6(1):12816-1281617
Electroosmotic flow that involves one fluid displacing another fluid is commonly encountered in various microfludic applications and experiments, for example, current monitoring technique to determine zeta potential of microchannel. There is experimentally observed anomaly in such flow, namely, the displacement time is flow direction dependent, i.e., it depends if it is a high concentration fluid displacing a low concentration fluid, or vice versa. Thus, this investigation focuses on the displacement flow of two fluids with various concentration differences. The displacement time was determined experimentally with current monitoring method. It is concluded that the time required for a high concentration solution to displace a low concentration solution is smaller than the time required for a low concentration solution to displace a high concentration solution. The percentage displacement time difference increases with increasing concentration difference and independent of the length or width of the channel and the voltage applied. Hitherto, no theoretical analysis or numerical simulation has been conducted to explain this phenomenon. A numerical model based on finite element method was developed to explain the experimental observations. Simulations showed that the velocity profile and ion distribution deviate significantly from a single fluid electroosmotic flow. The distortion of ion distribution near the electrical double layer is responsible for the displacement time difference for the two different flow directions. The trends obtained from simulations agree with the experimental findings.  相似文献   

2.
End effects on the flow of heat, mass or electrical energy through a cylindrical rod were investigated analytically. Three of the eight analytical solutions were evaluated on a 36091 computer and compared with the results of a numerical analysis package dubbed “Heating III” to show that both compared favorably.Platinum and Hastelloy X rods were subjected to an electrical current applied via two electrodes, one covering one end and the application of the other being thesubject of this investigation. End effects based on voltage deviations of one part in a thousand or more were found to extend for a length to diameter ratio ranging from 0·6 to 1·9 depending upon whether the small electrode position being investigated was at the center of the end or on the periphery of the end. Calculated results from Heating III showed excellent agreement with the experimental results.In addition to their reported applications, the analytical solutions represent a contribution to a neglected area of applied mathematical physics and as such should prove equally useful in other areas that are dynamically analogous. Also the discontinuous infinite integral technique of solution has proven to be a very powerful one.  相似文献   

3.
The present work describes the operation and simulation of a microfluidic laminar-flow mixer. Diffusive mixing takes place between a core solution containing lipids in ethanol and a sheath solution containing aqueous buffer, leading to self assembly of liposomes. Present device architecture hydrodynamically focuses the lipid solution into a cylindrical core positioned at the center of a microfluidic channel of 125 × 125-μm2 cross-section. Use of the device produces liposomes in the size range of 100–300 nm, with larger liposomes forming at greater ionic strength in the sheath solution and at lower lipid concentration in the core solution. Finite element simulations compute the concentration distributions of solutes at axial distances of greater than 100 channel widths. These simulations reduce computation time and enable computation at long axial distances by utilizing long hexahedral elements in the axial flow region and fine tetrahedral elements in the hydrodynamic focusing region. Present meshing technique is generally useful for simulation of long microfluidic channels and is fully implementable using comsol Multiphysics. Confocal microscopy provides experimental validation of the simulations using fluorescent solutions containing fluorescein or enhanced green fluorescent protein.  相似文献   

4.
The T-shaped microchannel system is used to mix similar or different fluids, and the laminar flow nature makes the mixing at the entrance junction region a challenging task. Acoustic streaming is a steady vortical flow phenomenon that can be produced in the microchannel by oscillating acoustic transducer around the sharp edge tip structure. In this study, the acoustic streaming is produced using a triangular structure with tip angles of 22.62°, 33.4°, and 61.91°, which is placed at the entrance junction region and mixes the inlets flow from two directions. The acoustic streaming flow patterns were investigated using micro-particle image velocimetry (μPIV) in various tip edge angles, flow rate, oscillation frequency, and amplitude. The velocity and vorticity profiles show that a pair of counter-rotating streaming vortices were created around the sharp triangle structure and raised the Z vorticity up to 10 times more than the case without acoustic streaming. The mixing experiments were performed by using fluorescent green dye solution and de-ionized water and evaluated its performance with the degree of mixing (M) at different amplitudes, flow rates, frequencies, and tip edge angles using the grayscale value of pixel intensity. The degree of mixing characterized was found significantly improved to 0.769 with acoustic streaming from 0.4017 without acoustic streaming, in the case of 0.008 μl/min flow rate and 38 V oscillation amplitude at y = 2.15 mm. The results suggested that the creation of acoustic streaming around the entrance junction region promotes the mixing of two fluids inside the microchannel, which is restricted by the laminar flow conditions.  相似文献   

5.
In this study, we show the importance of extensional rheology, in addition to the shear rheology, in the choice of blood analog solutions intended to be used in vitro for mimicking the microcirculatory system. For this purpose, we compare the flow of a Newtonian fluid and two well-established viscoelastic blood analog polymer solutions through microfluidic channels containing both hyperbolic and abrupt contractions∕expansions. The hyperbolic shape was selected in order to impose a nearly constant strain rate at the centerline of the microchannels and achieve a quasihomogeneous and strong extensional flow often found in features of the human microcirculatory system such as stenoses. The two blood analog fluids used are aqueous solutions of a polyacrylamide (125 ppm w∕w) and of a xanthan gum (500 ppm w∕w), which were characterized rheologically in steady-shear flow using a rotational rheometer and in extension using a capillary breakup extensional rheometer (CaBER). Both blood analogs exhibit a shear-thinning behavior similar to that of whole human blood, but their relaxation times, obtained from CaBER experiments, are substantially different (by one order of magnitude). Visualizations of the flow patterns using streak photography, measurements of the velocity field using microparticle image velocimetry, and pressure-drop measurements were carried out experimentally for a wide range of flow rates. The experimental results were also compared with the numerical simulations of the flow of a Newtonian fluid and a generalized Newtonian fluid with shear-thinning behavior. Our results show that the flow patterns of the two blood analog solutions are considerably different, despite their similar shear rheology. Furthermore, we demonstrate that the elastic properties of the fluid have a major impact on the flow characteristics, with the polyacrylamide solution exhibiting a much stronger elastic character. As such, these properties must be taken into account in the choice or development of analog fluids that are adequate to replicate blood behavior at the microscale.  相似文献   

6.
For the diagnosis of biochemical reactions, the investigation of microflow behavior, and the confirmation of simulation results in microfluidics, experimentally quantitative measurements are indispensable. To characterize the mixing and reaction of fluids in microchannel devices, we propose a mixing quality index (Mqi) to quantify the cross-sectional patterns (also called mixing patterns) of fluids, captured with a confocal-fluorescence microscope (CFM). The operating parameters of the CFM for quantification were carefully tested. We analyzed mixing patterns, flow advection, and mass exchange of fluids in the devices with overlapping channels of two kinds. The mixing length of the two devices derived from the analysis of Mqi is demonstrated to be more precise than that estimated with a commonly applied method of blending dye liquors. By means of fluorescence resonance-energy transfer (FRET), we monitored the hybridization of two complementary oligonucleotides (a FRET pair) in the devices. The captured patterns reveal that hybridization is a progressive process along the downstream channel. The FRET reaction and the hybridization period were characterized through quantification of the reaction patterns. This analytical approach is a promising diagnostic tool that is applicable to the real-time analysis of biochemical and chemical reactions such as polymerase chain reaction (PCR), catalytic, or synthetic processes in microfluidic devices.  相似文献   

7.
An electro-osmosis (EOS) diode pumping platform capable of culturing cells in fluidic cellular micro-environments particularly at low volume flow rates has been developed. Diode pumps have been shown to be a viable alternative to mechanically driven pumps. Typically electrokinetic micro-pumps were limited to low-concentration solutions (≤10 mM). In our approach, surface mount diodes were embedded along the sidewalls of a microchannel to rectify externally applied alternating current into pulsed direct current power across the diodes in order to generate EOS flows. This approach has for the first time generated flows at ultra-low flow rates (from 2.0 nl/s to 12.3 nl/s) in aqueous solutions with concentrations greater than 100 mM. The range of flow was generated by changing the electric field strength applied to the diodes from 0.5 Vpp/cm to 10 Vpp/cm. Embedding an additional diode on the upper surface of the enclosed microchannel increased flow rates further. We characterized the diode pump-driven fluidics in terms of intensities and frequencies of electric inputs, pH values of solutions, and solution types. As part of this study, we found that the growth of A549 human lung cancer cells was positively affected in the microfluidic diode pumping system. Though the chemical reaction compromised the fluidic control overtime, the system could be maintained fully functional over a long time if the solution was changed every hour. In conclusion, the advantage of miniature size and ability to accurately control fluids at ultra-low volume flow rates can make this diode pumping system attractive to lab-on-a-chip applications and biomedical engineering in vitro studies.  相似文献   

8.
In this investigation the first series of tests consisted of obtaining zero flux in two varieties of steel from various maximum flux values and by three different methods. B-H curves were obtained after demagnetization by the three methods and from the maximum value of these B-H curves hysteresis loops were taken.One method of demagnetizing was that suggested by the U. S. Bureau of Standards, which consists of current reversals through a magnetizing winding, in which current values are gradually reduced to zero. The second method consisted of applying a negative current of such an amount that the resultant value of flux would be such that when the current was released the flux value would go to zero. The third method was substantially the same as the second except the zero flux was approached from the direction of opposite polarity.The resultant B-H curves proved to be different from each other and to depend upon not only their origin but the previous history of the material as regards methods of demagnetization.The areas between the curves were evaluated.It was found that in cases of two of the three typical B-H curves the B-log H curves gave straight lines for part of the range involved.A second series of tests consisted of taking a set of hysteresis loops and repeating the determinations, using a greater number of steps to find if by taking a greater number of steps there might be a greater indicated energy loss. The results of this group of tests were not sufficiently definite to warrant conclusions.  相似文献   

9.
This paper presents a solution for a semi-infinite plate containing a deep semi-elleptic edge notch when the plate is under a uniaxial tension parallel to the edge. The solution is a direct solution composed of three parts, which is developed from Howland's method of successive approximations through two significant modifications. It is found that the present solution is more adaptable to numerical computation with regard to the depth ratio of the notch than other solutions known to exist. Numerical values of the stress concentration are given for a depth ratio up to 10.  相似文献   

10.
We have performed microfluidic experiments with erythrocytes passing through a network of microchannels of 20–25 μm width and 5 μm of height. Red blood cells (RBCs) were flowing in countercurrent directions through microchannels connected by μm pores. Thereby, we have observed interesting flow dynamics. All pores were blocked by erythrocytes. Some erythrocytes have passed through pores, depending on the channel size and cell elasticity. Many RBCs split into two or more smaller parts. Two types of splits were observed. In one type, the lipid bilayer and spectrin network were cut at the same time. In the second type, the lipid bilayer reconnected, but the part of spectrin network stayed outside the cell forming a rope like structure, which could eventually break. The microporous membrane results in multiple breakups of the cells, which can have various clinical implications, e.g., glomerulus hematuria and anemia of patients undergoing dialysis. The cell breakup procedure is similar to the one observed in the droplet breakage of viscoelastic liquids in confinement.  相似文献   

11.
彭凡 《科教文汇》2020,(14):62-63
在计算方法课程教学中融入理论力学、材料力学知识内容,提出延拓性教学的三个层次:一是由力学问题认识基本数学问题的来源与背景;二是掌握基本数学模型的数值求解方法与理论;三是通过编程实验环节将具体力学问题转化成数学模型求数值解。近几年的课程教学效果调查表明,采用延拓性教学法能有效提高学生的学习主动性、综合应用所学知识分析问题和解决问题的能力。  相似文献   

12.
Problem-solving by everyday individuals is thought to occur as a two-step process. First, an individual identifies or formulates a problem, followed by entering into a subsequent search to find the best solution. Here, however, we consider an alternative process that everyday individuals may use for solution finding first theorized by von Hippel and von Krogh (2016). Specifically, von Hippel and von Krogh proposed that everyday individuals may sometimes discover a solution and the need it satisfies simultaneously without the need for apriori problem formation, a cognitive process they called “need-solution pair recognition”. Utilizing a rich literature from psychology and neuroscience, we propose that seemingly spontaneous discoveries found by need-solution pair recognition are natural products of the object recognition system and its underlying mechanisms. This view asserts that on encountering an object and reasoning how it might be used (i.e. functional object understanding), an individual's perception of an object may culminate in recognizing the object as a solution, and in some cases, as a solution to a problem previously unknown to him or her, thus bypassing formal problem-formulation and active solution searching entirely. To empirically test this view, we manipulated the ability of everyday individuals to functionally reason about objects while we examined the spontaneous occurrence of solutions found by either need-solution pair recognition or traditional problem-first problem-solving. Consistent with our hypothesized mechanism, our results indicate that need-solution pair recognition occurs more frequently when constraints on functional object understanding are reduced. That is, we found that needsolution pair discoveries outpaced solutions found from traditional problem solving, in environments with unfamiliar objects, where participants were not directed to solve specific problems. Our results provide clear evidence that everyday individuals in the household sector do not always innovate through traditional problem-solving processes, but instead may arrive at solutions as they recognize and reason about objects. Implications for research and practice in household innovation, and for innovation more generally are considered.  相似文献   

13.
Electro-osmotic flow (EOF) pumps are attractive for fluid manipulation in microfluidic channels. Open channel EOF pumps can produce high pressures and flow rates, and are relatively easy to fabricate on-chip or integrate with other microfluidic or electrical components. An EOF pump design that is conducive to on-chip fabrication consists of multiple small channel arms feeding into a larger flow channel. We have fabricated this type of pump design using a thin film deposition process that avoids wafer bonding. We have evaluated pumps fabricated on both silicon and glass substrates. Consistent flow rate versus electric field were obtained. For the range of 40–400 V, flow rates of 0.19–2.30 μL∕min were measured. Theoretical calculations of pump efficiency were made, as well as calculations of the mechanical power generated by various pump shapes, to investigate design parameters that should improve future pumps.  相似文献   

14.
For inconsistent initial value problem of singular linear system, passing from classical solution to distributional solution is formulated as a process of embedding the system with initial condition into distribution space. Three technical notions, function space on R+ with given initial value, consistent extension of the pair of the function space and input, and order exchange of the two operations of embedding and differentiating, result in a rigorous definition of distributional solution.  相似文献   

15.
Optofluidic tweezer on a chip   总被引：1，自引：0，他引：1  
A novel method to realize an optical tweezer involving optofluidic operation in a microchannel is proposed. To manipulate the optical tweezer, light from an optical fiber is passed through both PDMS (polydimethylsiloxane)-air surface lenses and an optofluidic region, which is located in a control channel. Two liquids with different refractive indices (RIs) are introduced into the control channel to form two different flow patterns (i.e., laminar and segmented flows), depending on the liquid compositions, the channel geometry, and the flow rates. By altering the shapes of the interface of the two liquids in the optofluidic region, we can continuously or intermittently control the optical paths of the light. To demonstrate the functionality of the proposed method, optical tweezer operations on a chip are performed. Changing the flow pattern of two liquids with different RIs in the optofluidic region results in successful trapping of a 25 μm diameter microsphere and its displacement by 15 μm.  相似文献   

16.
内孤立波是陆架陆坡区一种常见的现象。本文借助于一个两层非静力近似模式（Gerkema,1996）模拟分析了内孤立波在陆架生成及传播特性,并与已有的观测资料进行了比较分析。结果表明,正压潮与地形相互作用激发生成内潮,内潮在向岸传播过程中,由于正压潮流及自身的非线性作用而发生分裂,受频散作用的影响进一步演化成内孤立子波列。而对于离岸传播的内潮,由于较强的频散效应,非线性与频散在较大尺度上达到平衡,从而使得波形呈现椭圆余旋波的特征。  相似文献   

17.
Multi-electron reaction can be regarded as an effective way of building high-energy systems (>500 W h kg−1). However, some confusions hinder the development of multi-electron mechanisms, such as clear concept, complex reaction, material design and electrolyte optimization and full-cell fabrication. Therefore, this review discusses the basic theories and application bottlenecks of multi-electron mechanisms from the view of thermodynamic and dynamic principles. In future, high-energy batteries, metal anodes and multi-electron cathodes are promising electrode materials with high theoretical capacity and high output voltage. While the primary issue for the multi-electron transfer process is sluggish kinetics, which may be caused by multiple ionic migration, large ionic radius, high reaction energy barrier, low electron conductivity, poor structural stability, etc., it is urgent that feasible and versatile modification methods are summarized and new inspiration proposed in order to break through kinetic constraints. Finally, the remaining challenges and future research directions are revealed in detail, involving the search for high-energy systems, compatibility of full cells, cost control, etc.  相似文献   

18.
Micromixer based on viscoelastic flow instability at low Reynolds number   总被引：1，自引：0，他引：1  
We exploited the viscoelasticity of biocompatible dilute polymeric solutions, namely, dilute poly(ethylene oxide) solutions, to significantly enhance mixing in microfluidic devices at a very small Reynolds number, i.e., Re≈0.023, but large Peclet and elasticity numbers. With an abrupt contraction microgeometry (8:1 contraction ratio), two different dilute poly(ethylene oxide) solutions were successfully mixed with a short flow length at a relatively fast mixing time of <10 μs. Microparticle image velocimetry was employed in our investigations to characterize the flow fields. The increase in velocity fluctuation with an increase in flow rate and Deborah number indicates the increase in viscoelastic flow instability. Mixing efficiency was characterized by fluorescent concentration measurements. Our results showed that enhanced mixing can be achieved through viscoelastic flow instability under situations where molecular-diffusion and inertia effects are negligible. This approach bypasses the laminar flow limitation, usually associated with a low Reynolds number, which is not conducive to mixing.  相似文献   

19.
Control problems in Hilbert spaces are treated in a measure-theoretical framework; instead of dealing with a set of admissible trajectory-control pairs, a set of measures defined by the boundary conditions and the differential equations of the problem are considered. The concept of weak controllability is introduced; a system has this property if every pair of initial and final points, (ta,xa) and (tb,xb) can be weakly joined; this is possible if a set of linear equalities involving measures has a solution. In turn, this is shown to be equivalent to the possibility of extending a linear functional in a positive manner. Necessary and sufficient conditions for controllability are derived, and applied to the study of a finite-dimensional system with the control appearing linearly.  相似文献   

20.
This paper deals particularly with those instruments of the index-and-scale and value-controlling types of the class of non-integrating instruments, as distinguished from integrating instruments and those used for comparison purposes strictly. Independent corroboration is adduced regarding the general characteristics of the hysteresis phenomena discussed in the author's earlier paper on the variance of measuring instruments.It is shown that the area of the hysteresis loop obtained on complete cyclic calibration is a measure of the energy dissipated in the operation of the instrument and that the smallness of the area of this loop, which may be used as a factor in a function exactly analogous to the resiliency in the case of other quasielastic bodies, is a measure of the excellence of the instrument as regards the reproducibility or invariance of its indications, so far as concerns mechanical sources of variation. The specific properties of the hysteresis loop are set down analytically and the physical nature and causes of the imperfect resilience of instruments are discussed in detail.Since the motion of an index or pointer through a displacement implies the existence of a motive force to bring about the deflection, in the presence of an equivalent reactive or restoring force opposing it, the essentials of a work diagram apparently always exist in the results of a properly perforined cyclic calibration. Indications are given of the methods to be followed in the process of reducing the results of the calibration to such form that the loop obtained correctly represents energy dissipation during a cycle, typifying an integral of the form
∫ByDy [Ø1 (Y)?Ø2 (Y)] dy
when By and Dy are the projections of the extremities B and D of the loop on the, axis of deflection or reading.The restrictions which surround the carrying out of cyclic calibrations in a manner calculated to obtain results of the character required are discussed, including the requirements of slow, aperiodic change of the variables, unreversed between the chosen extreme turning points, and accomplished in the absence of jarring or vibration. Attention is directed to the necessity of accustoming the instrument to the particular cycle over which it is to be calibrated, to the end of regularizing its performance.Both the form and area of the hysteresis loop should be observed, in order to arrive at regional as well as aggregate effects of the causes producing the variance. The amount and scope of the effects of vibratory treatment in modifying and diminishing the causes of lag are indicated.The possible causes of the lag known to exist in. the case of instruments using a surface of discontinuity between fluids as the indicating element are discussed, and it is shown that known phenomena perhaps hitherto unrecognized in their relation to instrument design and calibration may account for the variancy noted. Detailed experimental consideration of these factors is hoped for.Comparison of instrument performances on the basis of the hysteresis loss requires equivalence of the ranges of operation, or possibly reduction of the results on the basis of information not vet available in a form, capable of general application.The resiliency determination opens up a field for type-testing and selection of instruments on a basis quite discrete from that pertaining to the ordinary methods of calibration, in that the former permits selection between types of instruments rather than between individuals, making clearly discriminable the differences between the characteristics of given operating principles, designs of mechanical details, or qualities of workmanship. The more general methods of diminishing variance educible from the foregoing considerations are indicated.  相似文献   

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