共查询到20条相似文献，搜索用时 125 毫秒
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追求卓越,拒绝平庸——正是北京大学基础医学院免疫学系葛青教授的真实写照。为此,她留美多年,在麻省理工大学开展博士后研究,专注于免疫学科研工作,在病毒特异性siRNA的设计及其在体内外的功能筛选和验证、T细胞稳态增殖及记忆T细胞分化等的分子调控机制中做了大量研究。2009年回国后,她继续在免疫细胞发育和分化领域展开深入探索,为我 相似文献
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《中国科学基金》2015,(1)
国家自然科学基金重大项目"自身免疫识别与应答的机制及调节研究"围绕自身免疫识别与应答的机制及调节这一免疫学重大基础科学问题,以活化淋巴细胞来源的双链DNA(ALDDNA)免疫小鼠诱导系统性红斑狼疮(SLE)为自身免疫病原型动物模型,研究了DNA的化学修饰及自身免疫识别机制、调节性抗体的产生机制及其在自身免疫应答与调节中的作用、Treg亚群格局的调控及其在自身免疫应答中的作用这3个子科学问题。经过4年研究,以ALD-DNA和活化T细胞膜翻转脂筏相关蛋白为二大自身抗原,在dsDNA免疫原性的化学基础、DNA免疫识别及应答规律、抗T细胞抗体的产生和调节机制、Treg的调控及对自身免疫的调节等方面取得突破性进展,提出基于SAP,Notch1,miR155,Foxo3a,CRT,W8,NK007等的治疗SLE的新策略。 相似文献
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免疫应答的基本模式及其起源 总被引：3，自引：0，他引：3
免疫应答基本模式为体内存在静息免疫细胞库，抗原选择性激活其中特异性细胞，活化的有关细胞胺阶段发出和接受调控信号，增殖分化形成效应细胞和因子，主要诱使作用对象发生转化或清除。这揭示最初免疫应答与发育调控有关。 相似文献
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近年来发现的效应性辅助性T细胞新亚群Th17细胞,是独立于Th1和Th2细胞的第三类Th细胞亚群.Th17细胞与自身免疫病等多种疾病相关.该文从Th17细胞的功能以及其与疾病的相互关系等方面对其研究进展进行综述. 相似文献
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<正>2016年6月14日,国际权威学术期刊Immunity在线发表了北京师范大学刘光伟教授领导团队的研究成果"Histone deacetylase SIRT1negatively regulates the differentiation of interleukin-9-producing CD4+T cells"(组蛋白去乙酰化酶SIRT1负性调控Th9细胞分化)。该研究揭示了SIRT1在Th9细胞分化的调控效应和机制,为探讨Th9细胞相关哮喘和肿瘤疾病的发病 相似文献
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刘强 《科技成果管理与研究》2013,(6):90-90
中科院微生物研究所研究员方敏博士长期从事病毒免疫学的研究,其主要研究领域为病毒感染后机体的免疫系统是如何作用来控制病毒的感染,以及基于免疫学方面的亚病毒疫苗的研究。近年来,方敏博士的研究主要集中在NK细胞领域,她研究揭示了NK细胞在抗痘病毒感染中的分子识别机制与免疫调控功能,并发现NK细胞功能随年龄的增长而下降从而导致小鼠丧失抵抗病毒感染的能力,这对于人们认识免疫衰老的机制有重要推动作用。 相似文献
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生物学中的许多现象如细胞周期调控、信号转导、中间代谢、复制、转录、翻译、剪切等均受蛋白质作用的调控，有些蛋白质结合紧密，而有些蛋白质作用短暂，从不同方式调控着生物细胞活动，包括增殖、分化和死亡，为使细胞的生命活动过程正常进行，应当确保蛋白质问的作用正常稳定。研究两种蛋白之间相互作用通常采用生化技术如偶联、免疫共沉淀等 相似文献
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自20世纪60年代人类首次利用流产胎儿组织建立人二倍体细胞系（HDCs）以来,人胚胎来源的细胞就被广泛应用于生物医学研究领域,为提高人类健康水平作出了重大贡献。近年来,包括人胚干细胞在内的人多能干细胞在再生医学领域展现出巨大的治疗潜力,并受到各国政府和社会公众的高度关注。同时,由于历史文化、宗教信仰、伦理道德等多方面的因素,人胚胎来源细胞的研究和应用一直存在较大争议。文章通过介绍人胚胎来源细胞在生物医药领域研究应用的历史和进展,探究不同国家监管制度的演进,以期为完善我国对该领域的监管框架和技术要求提供有益参考。 相似文献
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Induction of diabetes by Streptozotocin in rats 总被引：1，自引：0，他引：1
A. Akbarzadeh D. Norouzian M. R. Mehrabi Sh. Jamshidi A. Farhangi A. Allah Verdi S. M. A. Mofidian B. Lame Rad 《Indian journal of clinical biochemistry : IJCB》2007,22(2):60-64
The objective of this study is to induce experimental diabetes mellitus by Streptozotocin in normal adult Wistar rats via
comparison of changes in body weight, consumption of food and water, volume of urine and levels of glucose, insulin and C-peptide
in serum, between normal and diabetic rats. Intra-venous injection of 60mg/kg dose of Streptozotocin in adult wistar rats,
makes pancreas swell and at last causes degeneration in Langerhans islet beta cells and induces experimental diabetes mellitus
in the 2–4 days. Induction of experimental diabetes mellitus is indeed the first step in the plan of purification of pancreatic
Langerhans islet cells of normal rats for transplanting under the testis subcutaneous of experimentally induced diabetic rats.
Streptozotocin induces one type of diabetes which is similar to diabetes mellitus with non-ketosis hyperglycemia in some animal
species. For induction of experimental diabetes in male adult rats weighted 250–300 grams (75–90 days), 60mg/kg of Streptozotocin
was injected intravenously. Three days after degeneration of beta cells, diabetes was induced in all animals. The diabetic
and normal animals were kept in the metabolic cages separately and their body weight, consumption of food and water, urine
volume, the levels of serum glucose, insulin and C-peptide quantities in all animals were measured and then these quantities
were compared. For a microscopic study of degeneration of Langerhans islet beta cells of diabetic rats, sampling from pancreas
tissue of diabetic and normal rats, staining and comparison between them, were done. Induction of diabetes with Streptozotocin
decreases Nicotinamide-adenine dinucleotide (NAD) in pancreas islet beta cells and causes histopathological effects in beta
cells which probably intermediates induction of diabetes. In this study, we used Streptozotocin for our experiments in induction
of experimental diabetes mellitus. After Induction of diabetes, consumption of food and water, volume of urine and glucose
increased in the diabetic animals in comparison with normal animals, but the weight of body and the volume of insulin and
C-peptide decreased in the diabetic animals. Sampling and staining of pancreas tissue of diabetic and normal rats showed that
the Langerhans islet beta cells of diabetic rats have been clearly degenerated. In three days, Streptozotocin makes pancreas
swell and at last causes degeneration in Langerhans islet beta cells and induces experimental diabetes. It also changes normal
metabolism in diabetic rats in comparison with normal rats. Consumption of water and food, volume of urine, serum glucose
increases in diabetic animals in comparison with normal rats but the levels of serum insulin, C-peptide and body weight decreases. 相似文献
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A. Akbarzadeh D. Noruzian Sh. Jamshidi A. Farhangi M. R. Mehrabi B. Lame Rad M. Mofidian A. Allahverdi 《Indian journal of clinical biochemistry : IJCB》2007,22(1):71-76
Insulin injection is the main way to combat against insulin-dependent diabetes mellitus effects. Today in some laboratories
in the world, the investigators are trying to find some treatments for this disease with insulin-secreting pancreatic islet
cells transplantation. Donor tissue in each step of work was prepared from 36 adult male wistar Rats weighted 250–300 grams
(75–90 days). Transplantation was done in rats after 2–4 weeks induction of diabetes with 60mg/kg of streptozotocin injection
by intravenous method. Encapsulation of pancreatic islet cells allows for transplantation in the absence of immunosuppression.
This technique that is called “immunoisolation” is based on the principle that transplanted tissue is protected for the host
immune system by an artificial or natural membrane. In this study, the levels of insulin, C-peptide and glucose in diabetic
rats have been reached to normal range as compared to un-diabetic rats in 20 days after transplantation of islet cells, so
that testis is immunoisolated place for islet cells transplantation. Inside the testis subcutaneously and intrapretoneally
implantation of pure islet cells graft, that is a natural immunoisolation method, rapidly and permanently normalized the diabetic
state of streptozocin-administered animals. 相似文献
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探讨TCTE3 mRNA在人肝癌细胞株中的表达水平及意义。采用实时荧光定量PCR技术分别检测TCTE3基因在常规培养或缺氧诱导（CoCl2为诱导剂）的人肝癌细胞株中的表达情况。TCTE3 mRNA在常规培养的人正常肝细胞株L02,肝癌细胞株Bel-7402、SMMC-7721、HepG2和QGY-7701中不表达或极低表达;但经缺氧诱导后,SMMC-7721细胞中的TCTE3 mRNA表达量逐渐升高,4h达到最大,随后逐渐降低,与HIF1a mRNA表达的曲线变化有一定相似之处,只是这种变化的时间要早于HIF1a基因。在缺氧状态下肝癌细胞TCTE3 mRNA表达增加,且这种上调表达可能对HIF1a mRNA的表达有一定的促进作用。 相似文献
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Simona Cernea Minodora Dobreanu 《Biochemia medica : ?asopis Hrvatskoga dru?tva medicinskih biokemi?ara / HDMB》2013,23(3):266-280
Diabetes is a complex, heterogeneous condition that has beta cell dysfunction at its core. Many factors (e.g. hyperglycemia/glucotoxicity, lipotoxicity, autoimmunity, inflammation, adipokines, islet amyloid, incretins and insulin resistance) influence the function of pancreatic beta cells. Chronic hyperglycaemia may result in detrimental effects on insulin synthesis/secretion, cell survival and insulin sensitivity through multiple mechanisms: gradual loss of insulin gene expression and other beta-cell specific genes; chronic endoplasmic reticulum stress and oxidative stress; changes in mitochondrial number, morphology and function; disruption in calcium homeostasis. In the presence of hyperglycaemia, prolonged exposure to increased free fatty acids result in accumulation of toxic metabolites in the cells (“lipotoxicity”), finally causing decreased insulin gene expression and impairment of insulin secretion. The rest of the factors/mechanisms which impact on the course of the disease are also discusses in detail.The correct assessment of beta cell function requires a concomitant quantification of insulin secretion and insulin sensitivity, because the two variables are closely interrelated. In order to better understand the fundamental pathogenetic mechanisms that contribute to disease development in a certain individual with diabetes, additional markers could be used, apart from those that evaluate beta cell function.The aim of the paper was to overview the relevant mechanisms/factors that influence beta cell function and to discuss the available methods of its assessment. In addition, clinical considerations are made regarding the therapeutical options that have potential protective effects on beta cell function/mass by targeting various underlying factors and mechanisms with a role in disease progression. 相似文献
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介绍了选用ＨＲＰ（辣根过氧化物酶）为标记物,ＭｇＳＯ４做稳定剂,制作健康小鼠脑切片,观察神经细胞突起运输的实验;为追踪研究神经传导路提供实验手段。 相似文献
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Azim Akbarzadeh Dariush Norouzian Ali Farhangi Mohammad Reza Mehrabi Shirin Jamshidi Davood Zare Morvarid Shafiei 《Indian journal of clinical biochemistry : IJCB》2008,23(1):57-61
Flow cytometry has been employed as a method to study homogeneity of isolated islet subpopulations. After collagenase digestion
of rat pancreas and elutriation of tissue fragments, islets were isolated and dissociated, and cells were analyzed and sorted
according to their low forward angle light scattering properties by using automated flow cytometry. A standardized procedure
was developed for the preparation of rat islet cell grafts for purification of islet cells. In this process, after collagenase
digestion of pancreas, islets were isolated, dissociated, identification by dithizone method and then with enzymatic procedure
by DNase and trypsin, the islet cells changed into single cells and beta cells were identified by immunofluorescence method
and then assayed by flow cytometry. Methods have been developed for the preparation of suspension of viable rat pancreatic
islet cells and their analysis and sorting in the fluorescence activated cell sorter (FACC IV, Becton Dickinson, Sunnyvale,
Ca). Flow cytometry of these cells indicated that there were 91% of beta cells in cell suspension. Most of the exocrine particles
were lost during digestion. Purified endocrine islet cell grafts were prepared by pure beta-cells, without endocrine non-beta
cells. The purified aggregates were devoid of endocrine non-beta cells and damaged cells. 相似文献
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Venkateshwari A Sri Manjari K Krishnaveni D Nallari P Vidyasagar A Jyothy A 《Indian journal of clinical biochemistry : IJCB》2011,26(2):136-139
Pancreatic fibrosis is a key pathological feature in the etiology of chronic pancreatitis that leads to obliteration of exocrine
and endocrine pancreatic tissues and its replacement by fibrous tissue resulting in clinical manifestations. Matrix metalloproteinase
9 is a member of the MMP family that is also known as gelatinase B, degrades type IV collagen of extracellular matrix and
basal membrane. The present study is aimed at evaluating the clinical significance of plasma concentration of MMP-9 in chronic
pancreatitis. The samples were obtained from 112 chronic pancreatitis patients and an equal number of age and sex matched
healthy controls. MMP-9 levels were quantitatively measured by ELISA assay. Statistical analysis was applied to test the significance
of results. The present study revealed a significant increase of plasma MMP 9 levels in chronic pancreatitis patients compared
to control subjects. Elevated levels were also observed in all the patient groups compared to control subjects with regard
to sex, age, addictions etc. MMP-9 degrades the type IV collagens in normal basement membrane, which in turn activates the
pancreatic stellate cells which promote the development of pancreatitic fibrosis. Thus, elevated plasma levels of MMP-9 may
act as a susceptibility factor for the development of chronic pancreatitis. 相似文献