共查询到20条相似文献,搜索用时 15 毫秒
1.
C. V. Divyambika S. Sathasivasubramanian G. Vani A. J. Vanishree N. Malathi 《Indian journal of clinical biochemistry : IJCB》2018,33(3):348-355
This study aimed to correlate the oxidative stress marker levels in saliva with the clinical stage based on mouth opening, fibrotic bands and histopathological grades of oral submucous fibrosis (OSF) patients. The study included patients clinically diagnosed with OSF (n = 63) and equal number of age and gender matched controls. Patients with OSF were defined by mouth opening stage, fibrotic bands and histopathological grades. Unstimulated saliva from both control and OSF patients were analysed for oxidative markers like lipid peroxides (LPO), non-enzymic antioxidants [reduced glutathione (GSH), vitamin A, vitamin E, vitamin C] and enzymatic antioxidants [glutathione peroxidase (GPx), superoxide dismutase (SOD)] and correlated with different stages and grades. Total salivary protein and LPO were significantly increased in OSF group with no significant change in the levels of GSH compared to controls. In OSF patients, a significant decrease in the levels of vitamins A, C and E was observed. The activities of salivary SOD and GPx were significantly decreased in OSF patients compared to controls. These changes significantly correlated with the increasing and differing grades of OSF that reflects increased oxidative stress with the progress of OSF. 相似文献
2.
The present study was undertaken to analyze the antioxidant (both enzymic and nonenzymic) activities of leaves of Ocimum sanctum hydroalcoholic extract against cadmium induced damage in albino rats. Oral administration of cadmium as CdCl2 (6.0 mg/kg body weight) led to significant elevation of lipid peroxidation (LPO) levels and significantly decreased Superoxide
Dismutase (SOD), Catalase (CAT), Glutathione Peroxidase (GPx), Reduced Glutathione (GSH) and Vitamin C (Ascorbate) levels.
Administration of Ocimum sanctum extract (100 mg/kg body weight, po) and (200 mg/kg body weight, po) before and after cadmium intoxication showed a significant
decrease in LPO levels and significant increase in SOD, CAT, GPx, GSH and Ascorbate levels. The results suggest that oral
administration of Ocimum sanctum extract provides significant protection against cadmium induced toxicity in Wistar albino rats. 相似文献
3.
Krishna Mohan Surapneni V. S. Chandrasada Gopan 《Indian journal of clinical biochemistry : IJCB》2008,23(1):41-44
The changes in the erythrocyte lipid peroxidation products (MDA), levels of glutathione (GSH), ascorbic acid and plasma vitamin
E (non enzymatic antioxidant parameters) and activities of antioxidant enzymes superoxide dismutase (SOD), glutathione peroxidase
(GPX), catalase in erythrocytes and plasma glutathione - S - transferase (GST) activity were estimated in patients with rheumatoid
arthritis. This work was undertaken to assess oxidative stress and anti oxidant status in patients with rheumatoid arthritis.
It was observed that there was a significant increase in erythrocyte MDA levels, activities of SOD, GPX, plasma GST and a significant decrease in erythrocyte GSH, ascorbic acid, plasma vitamin E levels and catalse activity in
patients with rheumatoid arthritis when compared to controls. The results of our study suggests higher oxygen free radical
production, evidenced by increased MDA and decreased GSH, ascorbic acid, vitamin E and Catalase activity, support to the oxidative
stress in rheumatoid arthritis. The increased activities of antioxidant enzymes may be a compensatory regulation in response
to increased oxidative stress. 相似文献
4.
Anisha Chauhan Nidhi Srivastva Parvesh Bubber 《Indian journal of clinical biochemistry : IJCB》2018,33(4):422-428
Thiamine or vitamin B1 is a well known coenzyme and nutrient necessary for the assembly and right functioning of several enzymes involved in the energy metabolism. The present study evaluates oxidative stress and prevalence of neurodegenerative conditions in the brain following TD. The study was carried out on mice (Musmusculus) in three groups, namely control and thiamine-deficient group for 8 (TD 8) and 10 (TD 10) days. Lipid peroxidation was determined in terms of reduced glutathione (GSH) and thiobarbituric acid reactive substance (TBARS). The level of antioxidant enzymes such as catalase (CAT), glutathione reductase, glutathione peroxidase (GPx), superoxide dismutase (SOD) and glutathione transferase (GST) were measured along with histopathological studies in all the groups. There was significant increase in the TBARS levels in group II (TD 8) and group III (TD 10) animals in comparison to controls (Group I). The GSH levels were found to be lower in both the treated groups. The level of antioxidant enzymes CAT (p < 0.001), glutathione reductase (p < 0.001), GPx (p < 0.001), SOD (p < 0.0001) were found to be significantly reduced in group III (TD 10) in comparison to controls. Histopathological studies showed moderated to extensive neuronal loss in group II and group III in comparison to control group. The increase in LPO and reduction in enzymes CAT, glutathione reductase, GPx, SOD, and GST following TD suggests mitochondrial dysfunction, neuronal loss acute oxidative stress that may impair the functioning of the brain along with the rise of neurodegenerative conditions in the affected animals. 相似文献
5.
A. K. Pradhan A. K. Shukla M. V. R. Reddy N. Garg 《Indian journal of clinical biochemistry : IJCB》2004,19(1):83-87
Oxidative stress was assesed by estimating lipid peroxidation product (LPO) in the form of thiobarbituric acid reactive substances
(TBARS), enzymatic antioxidants in the form of superoxide dismutase (SOD), catalase and nonenzymatic antioxidant vitamins
e.g. vitamin C, β carotene and vitamin E in either serum or plasma or erythrocytes in 190 cases of age related cataract in
the age group of 50–80 years. 190 cases were grouped into three morphological types namely, 73 cases of cortical, 77 cases
of posterior subcapsular and 40 cases of nuclear cataract and values of LPO and antioxidants were compared with 78 cases of
age matched healthy control groups. Plasma TBARS levels were cataract cases when compared with control groups. There were
no significant differences in the erythrocyte levels of catalase and plasma levels of Vit E between cataract cases and control
groups. No significant changes of parameters were seen among three different morphological types of age related cataract.
The present study shows that the oxidative stress may play an important role in the age related cataract. 相似文献
6.
Amit Kumar Mani Tiwari Abbas Ali Mahdi Fatima Zahra Sudarshna Sharma Mahendra Pal Singh Negi 《Indian journal of clinical biochemistry : IJCB》2012,27(3):246-252
To correlate blood lead levels (BLLs) and oxidative stress parameters in pregnant anemic women. A total of 175 pregnant women were found suitable and included for this study. Following WHO criteria, 50 each were identified as non-anemic, mild anemic and moderate anemic and 25 were severe anemic. The age of all study subjects ranged from 24–41 years. At admission, BLLs and oxidative stress parameters were estimated as per standard protocols and subjected with ANOVA, Pearson correlation analysis and cluster analysis. Results showed significantly (p < 0.01) high BLLs, zinc protoporphyrin (ZPP), oxidized glutathione (GSSG), lipid peroxide (LPO) levels while low delta aminolevulinic acid dehydratase (δ-ALAD), iron (Fe), selenium (Se), zinc (Zn), haemoglobin (Hb), haematocrit (Hct), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), red blood cell (RBC) count, reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT) and total antioxidant capacity (TAC) in all groups of anemic pregnant women as compared with non anemic pregnant women. In all groups of pregnant women, BLLs showed significant (p < 0.01) and direct association with ZPP, GSSG and LPO while inverse relation with δ-ALAD, Fe, Se, Zn, Hb, Hct, MCV, MCH, MCHC, RBC, GSH, SOD, CAT and TAC. Study concluded that low BLLs perturb oxidant-antioxidant balance and negatively affected hematological parameters which may eventually Pb to Fe deficiency anemia during pregnancy. 相似文献
7.
Eshrat M. Halim A. K. Mukhopadhyay 《Indian journal of clinical biochemistry : IJCB》2006,21(2):181-188
This study was carried out to see the effect of the aqueous extract ofOcitum sanctum Linn (Tulsi) with Vitamin E on biochemical parameters and retinopathy in the streptozotocin-induced diabetic albino male
rats. Adult albino male rats weighing 150–200 gm were made diabetic by intraperitoneal injection of streptozotocin in the
dose 60 mg/kg in citrate buffer (pH 6.3). The diabetic animals were left for one month to develop retinopathy. Biochemical
parameters like plasma glucose, oral glucose tolerance and glycosylated hemoglobin HbA1c, were measured along with lipid profile, and enzymes like glutathione peroxidase (GPX), lipid peroxidase (LPO), superoxide
dismutase (SOD), catalase (CAT) and glutathione-S-transferase (GST) in normal, untreated diabetic rats and diabetic rats treated
withOcimum sanctum L extracts and vitamin E. Fluorescein angiography test was done for assessing retinopathy. Results on biochemical parameters
were analyzed statistically by using ANOVA followed by Dunnet's ‘t’-test. A p-value of <0.05 was considered as significant.
Evaluation of biochemical profile in treated groups showed statistically significant reduction in plasma levels of glucose,
HbA1c, lipid profile and LPO, and elevation of GPX, SOD, CAT and GST. Treatment of the diabetic animals withOcimum sanctum and Vitamin E, alone and in combination for 16 weeks showed reversal of most of the parameters studied including plasma glucose
levels. Angiography showed improvement in retinal changes following combined antidiabetic treatment. 相似文献
8.
Raghunath R. Rai Madhavi S. Phadke 《Indian journal of clinical biochemistry : IJCB》2006,21(2):161-164
This study confirms the fact that in different respiratory disorders, the status of plasma oxidants and antioxidants shifts
from normal. The status of oxidants in plasma as represented by malondialdehyde (MDA) levels increased significantly in the
conditions of chronic obstructive pulmonary disease (COPD), emphysema, bronchiectasis and bronchial asthma. The two vitamin
antioxidants vitamin C and vitamin E showed decreased levels than in controls. In patients with COPD the endogenous antioxidant
viz. reduced glutathione (GSH) estimated from whole blood was comparable to that of control group, whereas in patients with
emphysema, bronchiectasis and bronchial asthma, GSH concentration was increased to that of control group. The activity of
enzyme superoxide dismutase (SOD) was significantly decreased in all study groups. Pulmonary function tests were found to
have no correlation with MDA and antioxidants 相似文献
9.
Kiran Dahiya A. D. Tiwari Vijay Shankar Simmi Kharb Rakesh Dhankhar 《Indian journal of clinical biochemistry : IJCB》2006,21(1):157-160
Phototherapy has been related to increased oxidative stress and lipid peroxidation. In the present study, thirty full term
jaundiced neonates with appropriate weight were analyzed before and after completion of phototherapy for malondialdehyde (MDA),
reduced glutathione (GSH), total thiols, vitamin C and superoxide dismutase (SOD) levels in hemolysate and albumin levels
in plasma. These parameters were analyzed in cord blood samples of 20 healthy neonates as control. It was observed that levels
of MDA were elevated significantly (p<0.001) in patients as compared to controls and that the levels increased significantly
after phototherapy (p<0.001). Levels of SOD were also found to be increased significantly as compared to controls and the
levels rose after phototherapy (p<0.001). On the other hand, the levels of non-enzymatic antioxidants such as GSH, total thiols
and vitamin C were significantly low (p<0.001) as compared to controls and the levels decreased significantly after phototherapy
(p<0.001). The plasma albumin levels also were found to be decreased significantly after phototherapy (p<0.01). Therefore,
phototherapy increases oxidative stress and should be used with care. 相似文献
10.
Eshrat Halim M. A. Hussain Kaiser Jamil Mala Rao 《Indian journal of clinical biochemistry : IJCB》2001,16(2):190-194
Effect of oral administration of 200 mg/Kg body weight of the aqueous extract ofOcimum sanctum (Tulsi) mixed with diet for eight weeks to diabetic (streptozotocin induced) rats was studied. There was significant reduction
in fasting blood glucose, serum lipid profile, lipid peroxidation products, (LPO) and improvement in glucose tolerance. The
aqueous extract also decreased LPO formation (thiobarbituric acid reactive substances TBARS) and increased antioxidant enzymes
superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), glutathione transferase (GT) and one antioxidant
reduced glutathione (GSH) in plasma and rat liver, lung, kidney and brain. The decrease in TBARS and increase in GSH, SOD,
CAT, GPX, and GT clearly shows the antioxidant property ofOcimum sanctum. 相似文献
11.
In this study antioxidant activity of methanol extract of rhizomes ofCurculigo orchioides (MEC) was investigated using carbon tetrachloride (CCl4)-intoxicated rat liver as the experimental model. The hepatotoxic rats were administered MEC for 90 days (daily, orally at
the dose of 70 mg per kg body weight). Lipid peroxidation (LPO) in CCl4-intoxicated rats was evidenced by a marked increment in the levels of thiobarbituric acid reactive substances (TBARS) and
diene conjugates (CD), and also a distinct diminution in glutathione (GSH) content in the liver. In CCl4+MEC—treated rats these biochemical parameters attained an almost normal level. The decreased activity of antioxidant enzymes,
such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX) and glutathione reductase (GRD) in CCl4—intoxicated rats, and its retrieval towards near normalcy in CCl4+MEC—administered rats revealed the efficacy of MEC in combating oxidative stress due to hepatic damage. Elevated level of
glutathione transferase(GTS) observed in hepatotoxic rats too showed signs of retuming towards normalcy in MEC co-administered
animals, thus corroborating the antioxidant efficacy of MEC. The findings provide a rationale for further studies on isolation
of active principles and its pharmacological evaluation. 相似文献
12.
M Maneesh H Jayalekshmi T Suma S Chatterjee A Chakrabarti T A Singh 《Indian journal of clinical biochemistry : IJCB》2005,20(1):129-130
Evidence of increased oxidative stress in patients of osteoarthritis in comparison with healthy control subjects was investigated
by measuring the thiobarbituric acid reactive substances (TBARS), vitamin C, reduced glutathione (GSH) and the activities
of superoxide dismutase (SOD), catalase and glutathione peroxidase (GPx) in erythrocytes. It was observed that osteoarthritis
patients were more susceptible to oxidative damage than controls as evident from increased TBARS and decreased ascorbic acid,
GSH, catalase and GPx in erythrocytes. Significant increase in SOD activity found in patients might be an adaptive response.
With the understanding of the role of antioxidants in arthritis, it is becoming increasingly clear that these agents seem
to be beneficial in osteoarthritis. 相似文献
13.
采用盆栽法考察不同质量浓度的Cd、Zn、Cu、Pb、As 5种重金属复合胁迫对酢酱草抗氧化酶活性的影响。结果显示随着重金属处理浓度梯度的增加,酢酱草过氧化物酶(POD)活性、抗坏血酸过氧化物酶(APX)活性、脯氨酸(PRO)含量及谷胱甘肽(GSH)含量先升高后降低;而超氧化物歧化酶(SOD)活性和过氧化氢酶(CAT)酶活性逐渐下降。结果表明酢酱草在重金属复合胁迫下能防御性的增加POD酶活性、SOD酶活性、PRO含量及GSH含量以减少逆环境的伤害,而重金属复合胁迫浓度过高则会导致酢酱草抗氧化酶系统受到明显损害,生长受到很大程度的抑制。 相似文献
14.
M. Smita K. Naidu A. N. Suryakar Sanjay C. Swami R. V. Katkam K. M. Kumbar 《Indian journal of clinical biochemistry : IJCB》2007,22(2):140-144
Cervical cancer (CaCx) is a global public health problem as it is the second most common cancer leading to the death of women
worldwide. Many references revealed that the low levels of antioxidants induce the generation of free radicals leading to
DNA damage and further mutations. In the present study attempt have been made to evaluate the levels of serum Lipid peroxide,
Nitric Oxide (NO.) Erythrocytic—Superoxide Dismutase (RBC-SOD), Vitamin-C, serum Copper (Cu) and serum Zinc (Zn). 120 patients were divided
in 4 groups according to the increasing CaCx stages i.e. stage I, II, III & IV respectively. All the patients were around
the age group of 25–65 years. 30 healthy women between the same age group were treated as controls. Highly significant increased
values of MDA, NO. and Cu were observed (p<0.001) whereas the activity of RBC-SOD, levels of Vitamin-C and Zn were significantly decreased in
CaCx patients as compared with healthy controls (p<0.001). Cu/Zn ratio was found to be altered in CaCx patients. From our
findings it can be concluded that the oxidative stress is induced among CaCx patients, which inturn increases the risk of
CaCx. 相似文献
15.
Harbans Lal Kiran Chugh Varinder Saini Jasbinder Kaur A. S. Saini 《Indian journal of clinical biochemistry : IJCB》1993,8(1):33-35
Glutathione-5-transferase (GST) and glutathione (GSH) levels were estimated in the lungs of 3 and 30 months old rats. GST activity was found to be significantly reduced in the old rats (30 months age) compared to the young rats (3 months age). In old rats, with methionine supplementation GST activity was increased whereas with vitamin C supplementation GSH levels were increased. The data show that the supplementation of methionine as well as of vitamin C have beneficial effect on the scavenging system and protect lungs against the free radical damage during ageing. 相似文献
16.
17.
Present study aimed to evaluate the protective role of the aqueous extract of Phyllanthus niruri (P. niruri) against nimesulide-induced
hepatic disoder in mice by determining levels of glutamate oxaloacetate transaminase (GOT), glutamate pyruvate transaminase
(GPT) and alkaline phosphatase (ALP) in serum and also by measuring the hepatic content of the antioxidant enzymes, superoxide
dismitase (SOD) and catalase (CAT); the free radical scavenger, reduced glutathione (GSH) and thiobarbituric acid reacting
substances (TBARS). Aqueous extract of P. niruri was administered either orally or intraperitoneally in different doses and
times as needed for the experiments. Intraperitoneal of the extract (100 mg/kg body weight for seven days) reduced nimesulide
(750 mg/kg body weight for 3 days) induced increased levels of GOT (37.0±1.8 units/ml in control group vs. 91.8±2.0 units/ml
in nimesulide treated group vs. 35.0±1.0 units/ml in extract treated group), GPT (30.0±2.1 units/ml in control group vs. 88.4±2.9
units/ml in nimesulide treated group vs. 34.1±1.8 units/ml in extract treated group), and ALP (7.86±0.47 KA units/ml in control
group vs. 23.80±0.60 KA units/ml in nimesulide treated group vs. 7.30±0.40 KA units/ml, in extract treated group) to almost
nomal. In addition, P. niruri restored the nimesulide induced alterations of hepatic SOD (550±20 units/mg total protein in
control group vs. 310±13 units/mg total protein in nimesulide treated group vs. 515±10 units/mg total protein in extract treated
group), CAT (99.5±2 units/mg total protein in control group vs. 25.0±1.5 units/mg total protein in nimesulide treated group
vs. 81.0±0.8 units/mg total protein in extract treated group), GSH (90±3 nmoles/mg total protein in control group vs. 17±4.2
nmoles/mg total protein in nimesulide treated group vs. 81±1 nmoles/mg total protein in extract treated group) and TBARS (measured
as MDA, 36.6±3.0 nmoles/g liver tissue in control group vs. 96.3±5.2 nmoles/g liver tissue in nimesulide treated group vs.
41.2±1.7 nmoles/g liver tissue in extract treated group) contents. Dose-dependent studies showed that the herb could protect
liver even if the nimesulide-induced injury is severe. Intraperitoneal administration of the extract showed better protective
effect than oral administration. Combining all, the data suggest that P. niruri possesses hepatoprotective activity against
nimesulide-induced liver toxicity and probably acts via an antioxidant defense mechanism. To the best of our knowledge, this
is the first report of the hepatoprotective action of P. niruri against nimesulide induced liver damage. 相似文献
18.
19.
Vinita Ailani R. C. Gupta Sunil Kumar Gupta Kapil Gupta 《Indian journal of clinical biochemistry : IJCB》2009,24(4):426-429
This study was conducted to find out the level of oxidative stress and effect of supplementation of vitamin C, D and Calcium
on levels of SOD, serum and urinary fluoride in children residing in endemic fluorosis area. For this the fluoride belt of
Jaipur district was selected. The parameters selected were Super oxide dismutase, serum fluoride and urinary fluoride. The
study was conducted on one hundred children, selected from four areas (25 from each area) consuming water containing 1.2,
2.4, 5.6 and 13.6 mg/l of fluoride. Drinking water fluoride, serum and urinary fluoride were measured by Ion selective electrode
method. Serum SOD by Xanthine oxidase method using kit of Ransod (kit cat. No. SD125). The post treatment values showed a
significant reduction in serum fluoride and SOD. Urinary fluoride levels increased significantly in post treatment stage.
The results revealed a normal SOD levels in all groups but an increasing trend was observed with increasing fluoride concentration.
Treatment with Calcium, Vitamin D and Vitamin C showed a significant reduction in serum fluoride and SOD and increase in urinary
fluoride. A high positive correlation between pretreatment and post treatment group was observed in serum fluoride, SOD and
urinary fluoride (P < 0.05). The study indicated an increasing oxidative stress in cases of fluorosis with increasing drinking
water fluoride concentration. Treatment with Calcium, Vitamin D and Vitamin C resulted a significant reduction in serum fluoride
and SOD and increase in urinary fluoride. 相似文献
20.
R. B. Bhogade A. N. Suryakar N. G. Joshi R. Y. Patil 《Indian journal of clinical biochemistry : IJCB》2008,23(3):233-237
Hemodialysis represents a chronic stress status for its recipients. Many hypotheses state that this may be associated with
oxidative stress. Thus, there may be deficiency of antioxidants like erythrocytic superoxide dismutase, catalase, vitamin
E or increased generation of free radicals like superoxide anions. A study was carried out to investigate oxidant and antioxidant
status in chronic renal failure patients undergoing hemodialysis and effect of vitamin E supplementation on these two status.
Blood samples were collected from patients before and after hemodialysis and from controls. The samples were analyzed for
quantitation of MDA as index of lipid peroxide, nitric oxide, vitamin E, vitamin C and enzymatic antioxidants namely erythrocyte
SOD and catalase. As compared to controls, the levels of serum MDA were significantly increased and activities of erythrocyte
SOD and catalase, levels of serum nitric oxide, serum vitamin E and plasma vitamin C were significantly decreased both before
and after hemodialysis. The efficiency of vitamin E therapy in hemodialysis patients was assessed by re-evaluating oxidant
and antioxidant status of same patients after supplementation of vitamin E. Vitamin E supplementation caused decrease in serum
MDA and increase in levels of serum nitric oxide, vitamin E, vitamin C and activities of erythrocytic SOD and catalase. Our
results suggest the presence of oxidative stress and the possible preventive role of vitamin E therapy in hemodialysis patients. 相似文献