共查询到18条相似文献，搜索用时 93 毫秒
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瞿宏杰 《襄樊职业技术学院学报》2003,2(2):40-41
随着转基因植物的大量问世，转基因植物的生物安全性越来越引起人们的重视。本就特基因植物的环境安全性和食品安全性两方面及管理现状进行概述。 相似文献
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植物基因工程是近20年来随着DNA重组技术、基因遗传转化技术及植物组织培养技术发展而兴起的生物技术．自转基因植物产生之日起，其研究发展迅速，被广泛地应用于各个领域．本文简要阐述了基因植物的研究概况、在农业中的应用及其生物安全性，并展望了植物基因工程的发展前景． 相似文献
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转基因抗病、虫植物应用及其安全性 总被引：2，自引：0，他引：2
转基因植物给人们的生产、生活带来了革命性的变化,生物安全问题如生态风险等成为一个国际争论焦点。本文综述了转抗病、虫基因应用的成就和生物安全性问题。转基因植物的应用,重点在于加强对其管理和安全应用。 相似文献
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转基因植物及其安全性问题 总被引：1，自引：0，他引：1
陈燕 《合肥联合大学学报》2005,15(1):12-15
介绍了转基因植物在全球和我国发展概况，介绍了转基因植物的几种目的基因：抗除草剂基因、抗病虫基因、改良作物品质基因，及其在农业上的应用，从转基因植物食品的安全性和转基因生态环境的安全性讨论了正确对待转基因技术的安全性问题。 相似文献
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随着DNA重组技术、基因遗传转化技术及植物组织培养技术的发展，植物基因工程日趋成熟．本文介绍了我国转基因植物研究现状，植物转基因常用的方法，转基因作物的安全性问题． 相似文献
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用微生物、植物、动物、人细胞或者专一性酶,通过生物方法将原料转化为特定产品的容器称为生物反应器（Bioreactor）.生物反应器的发展经历了三个阶段：细菌基因工程、细胞基因工程和转基因动物生物反应器.转基因动物生物反应器可以缩短重组蛋白的生产时间,具有很多优点,同时携带目的基因的阳性细胞克隆还可以长期冻存,长久作为核移植的供体,能够重复生产转基因动物. 相似文献
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转基因植物与植物转基因技术简介 总被引：5，自引：0，他引：5
随着DNA重组技术、基因遗传转化技术及植物组织培养技术的发展,植物基因工程日臻成熟。本文介绍了转基因植物的种类和植物基因转化技术。 相似文献
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Bt毒蛋白基因工程研究进展 总被引：4，自引：0，他引：4
近三十年来,以苏云金杆菌为基础的生物杀虫剂已在世界范围内商业化用于防治重要经济作物的害虫,有关Bt毒Pr基因的遗传,分子生物学和基因工程已取得显进展,本对Bt毒蛋白基因杀虫作用机理,基因分类,该基因的转基因植物研究状况作一简要综述。 相似文献
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We investigated the possibility of producing chicken alpha interferon (ChIFN-α) in transgenic plants.The cDNA encoding ChIFN-a was introduced into lettuce (Lactuca sativa L.) plants by using an agro-infiltration transient expression system.The ChIFN-α gene was correctly transcribed and translated in the lettuce plants according to RT-PCR and ELISA assays.Re-combinant protein exhibited antiviral activity in vitro by inhibition of vesicular stomatitis virus (VSV) replication on chicken embryonic fibroblast (CEF).The results demonstrate that biologically active avian cytokine with potential pharmaceutical ap-plications could be expressed in transgenic lettuce plants and that it is possible to generate interferon protein in forage plants for preventing infectious diseases of poultry. 相似文献
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An increasing number ofmonopartite begomoviruses are being identified that a satellite molecule (DNAβ) is required to induce typical symptoms in host plants. DNAβ encodes a single gene (termed βCl) encoded in the complementary-sense. We have produced transgenic Nieotiana benthamiana and N. tabaeum plants expressing the βC1 gene of a DNAβ associated with Tomato yellow leaf curl China virus (TYLCCNV), under the control of the Cauliflower mosaic virus 35S promoter. Transgenic plants expressing 13C1 showed severe developmental abnormalities in both species. Microscopic analysis of sections of both transgenic and non-transgenic N. tabaeum leaves showed abnormal outgrowths of transgenic N. tabaeum to be due to disorganizedcell division (hyperplasia) of spongy and palisade parenchyma. Immuno-gold labeling of sections with a polyclonal antibody against the βC1 protein showed that the 13C 1 protein accumulated in the nuclei of cells. The possible biological function of the βC1 1protein was discussed. 相似文献
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转基因烟草感染马铃薯Y病毒脉坏死株系后某些酶活性变化的比较研究 总被引：1，自引：0，他引：1
烟草感染马铃薯Y病毒脉坏死株系后,过氧化物酶活性升高,但非转基因敏感烟草升高的幅度大于转基因抗病烟草;同时,非转基固敏感烟草的苯丙氨酸解氨酶和多酚氧化酶活性均增高,并且都出现两个活性高峰,但转基因抗病烟草的苯丙氨酸解氨酶活性仅出现第一个活性高峰,随后其活性值降低并逐渐恢复至未接种时的水平,而多酚氧化酶活性则降低;在未感染PVYN的情况下,转基因抗病烟草的过氧化物酶、苯丙氨酸解氨酶和多酚氧化酶活性均比非转基因敏感烟草高． 相似文献
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Rao AQ Irfan M Saleem Z Nasir IA Riazuddin S Husnain T 《Journal of Zhejiang University. Science. B》2011,12(4):326-334
The phytochrome B (PHYB) gene of Arabidopsis thaliana was introduced into cotton through Agrobacterium tumefaciens. Integration and expression of PHYB gene in cotton plants were confirmed by molecular evidence. Messenger RNA (mRNA) expression in one of the transgenic lines,
QCC11, was much higher than those of control and other transgenic lines. Transgenic cotton plants showed more than a two-fold
increase in photosynthetic rate and more than a four-fold increase in transpiration rate and stomatal conductance. The increase
in photosynthetic rate led to a 46% increase in relative growth rate and an 18% increase in net assimilation rate. Data recorded
up to two generations, both in the greenhouse and in the field, revealed that overexpression of Arabidopsis thaliana PHYB gene in transgenic cotton plants resulted in an increase in the production of cotton by improving the cotton plant growth,
with 35% more yield. Moreover, the presence of the Arabidopsis thaliana PHYB gene caused pleiotropic effects like semi-dwarfism, decrease in apical dominance, and increase in boll size. 相似文献
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Transient expression of chicken alpha interferon gene in lettuce 总被引：1，自引：0，他引：1
We investigated the possibility of producing chicken alpha interferon (ChIFN-α) in transgenic plants.The cDNA encoding ChIFN-a was introduced into lettuce (Lactuca sativa L.) plants by using an agro-infiltration transient expression system.The ChIFN-α gene was correctly transcribed and translated in the lettuce plants according to RT-PCR and ELISA assays.Re-combinant protein exhibited antiviral activity in vitro by inhibition of vesicular stomatitis virus (VSV) replication on chicken embryonic fibroblast (CEF).The results demonstrate that biologically active avian cytokine with potential pharmaceutical ap-plications could be expressed in transgenic lettuce plants and that it is possible to generate interferon protein in forage plants for preventing infectious diseases of poultry. 相似文献