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首乌藤提取物对动物脂肪酸合酶的抑制及其减肥抑食作用 总被引：1，自引：0，他引：1
最新报道脂肪酸合酶（FAS）是治疗肥胖症的潜在靶部位。实验测定表明，中药首乌藤的提取物对FAS具有很强的抑制作用，半抑制浓度为0.61±0.024g/ml。同时测定，该提取物对FAS中的酮酰还原反应有强抑制，半抑制浓度为2.14±0.12g/ml，说明FAS中的酮酰还原酶是该提取物的作用部位之一。抑制动力学分析表明，首乌藤提取物对FAS的抑制和底物乙酰辅酶A、丙二酸单酰辅酶A之间皆呈非竞争性关系；和NADPH之间在低抑制剂浓度下的表现近似反竞争性，在高抑制剂浓度下接近竞争性的关系。推测首乌藤中可能有多种FAS抑制剂。用首乌藤提取物口服饲喂大鼠和小鼠，可明显减低实验动物的摄食量和体重；测定实验组大鼠肝脏的FAS活性，明显低于对照组。实验结果对于研究FAS抑制剂和抑制机制以及在防治肥胖症的应用上可能具有重要的价值。 相似文献
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四川边茶提取物对脂肪酸合酶的抑制作用 总被引：2，自引：0，他引：2
四川边茶（康砖）是黑茶的一个主要品种，所含茶多酚与绿茶和红茶不同。本研究利用酶动力学方法测定了边茶萃取物对脂肪酸合酶（Fatty Acid Synthase, FAS, E.C. 2.3.1.85）的抑制作用，近几年研究报道该酶是减肥和抑癌的双重潜在靶点。结果表明，边茶萃取物对FAS有可逆和不可逆抑制双重作用，最佳萃取溶剂为50％的乙醇，在常温下5 h即可达到最佳萃取效果。用开水泡茶的方法经两次浸取可提取出近60％的抑制FAS有效物，比绿茶的浸出效率约高1倍，但需较长的浸泡时间。边茶的新茶对脂肪酸合酶的抑制能力并不强，存放数年的边茶抑制能力最好，其后有所下降，但大部分抑制活性可保持20年以上，而30年以上保存期可能会使抑制能力明显下降。边茶提取物与底物乙酰辅酶A和丙二酸单酰辅酶A的竞争性质都是竞争性与非竞争性的混合型；对FAS中的酮酰还原反应的抑制不明显，这说明酮酰还原酶结构域并不是其主要作用位点；这些和绿茶及红茶都有不同。由实验结果分析边茶中的抑制剂不同于红茶和绿茶，可能是茶多酚中的茶褐素，其抑制能力高于儿茶素而接近茶黄素。这些结果表明边茶在减肥、抑癌等方面可能具有良好的应用前景。 相似文献
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最新报道脂肪酸舍酶（EC．2．3．1．85,Fattyacidsynthase,FAS）是治疗癌症和肥胖的双重靶部位。本文对24种抗肿瘤中药进行抑制FAS活性研究。实验结果表明,这24种中药中有17种具有抑制FAS生物活性（抑制率I〉40％）,其中9种中药抑制FAS活性较高（抑制率I〉70哟,为寻找无毒、低成本的FAS抑制剂提供依据。 相似文献
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草莓属蔷薇科植物。味甘、酸,性凉,无毒。草莓外观呈心形,果实鲜美红嫩,果肉柔软多汁,甘酸宜人,且有特殊的浓郁芳香。其不仅色、香、味俱佳,且营养价值极高,每百克新鲜草莓果肉中含糖8至9克、蛋白质0.4至0.6克,维生素C50至100毫克,其维生素C含量比苹果、葡萄高出7至10倍。它的苹果酸、柠檬酸、维生素B1、维生素B12, 相似文献
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《科技通报》2015,(9)
3.85 kg的孔石莼干粉末用甲醇提取得到320 g粗提物浸膏,浸膏被萃取分离为石油醚相、乙酸乙酯相、正丁醇相和蒸馏水相。对这4个相进行了高效液相色谱(HPLC)分析,然后对石油醚相和乙酸乙酯相进行了气相色谱-质谱(GC/MS)联用分析。用赤潮异弯藻进行各个分离组分的杀藻活性检测。活性检测表明石油醚相和乙酸乙酯相具有很强的杀藻活性,GC/MS分析鉴定其中的16种脂肪酸,其中不饱和脂肪酸占大部分。活性检测表明9,12,15-十八碳三烯酸、6,9,12,15-十八碳四烯酸、5Z,8Z,11Z,14Z-二十碳四烯酸、5Z,11Z,14Z,17Z-二十碳四烯酸和5Z,8Z,11Z,14Z,17Z-二十碳五烯酸具有很强的杀藻活性,其有效浓度在5 mg L-1以下。实验结果表明,孔石莼组织中含有多种不饱和脂肪酸,其中一些对赤潮异弯藻具有强烈的杀藻作用,不饱和脂肪酸可能是孔石莼组织内抑制赤潮微藻生长的他感物质重要组成成分之一。 相似文献
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《大科技．科学之谜》2013,(7):63
1.在球面几何中,三角形内角和—A.等于180度B.小于180度C.大于180度D.可能大于,也可能小于180度2.下列关于献血的说法,正确的是—A.献血会对身体造成很大伤害B.献血对于肥胖症患者有一定好处C.献血不分老幼,都可以献D.献血对于心脏病患者有一定好处3.神奇的等离子推进器的燃料其实是—A.液态氢B.电C.太阳能D.惰性气体氙4.下列关于云计算的说法,错误的是—A.云计算不需要服务器B.云计算需要互联网支持C.云计算节省了电费D.云计算提高了设备使 相似文献
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他汀类药物均为3-羟基-3-甲基戊二酰辅酶A还原酶抑制剂,这类药物不但可以通过抑制HMG-CoA还原酶减少肝细胞的合成以及储存胆固醇,提高低密度脂蛋白胆固醇代谢清除速度来降低人体内血清胆固醇的水平外,还具有改善血管的内皮功能、抑制炎症反应、抑制血小板聚集、抑制血栓形成、控制粥样硬化斑块形成以及抑制人体心脏电生理重塑等重要临床作用。为探讨他汀类药物在心血管疾病中的临床应用,本文对他汀类药物的临床药理等作用进行回顾性总结。 相似文献
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目的:通过系统预法对壮药三叶香茶菜乙酸乙酯部位浸膏中可能含有的化学成分进行预实验,初步探索三叶香茶菜乙酸乙酯部位浸膏的化学成分。方法:三叶香茶菜乙酸乙酯部位浸膏用水、乙醇和石油醚提取,取滤液进行研究,通过多种指示剂和显色剂的颜色或沉淀反应,对三叶香茶菜乙酸乙酯部位浸膏可能含有的化学成分进行初步研究。结论:三叶香茶菜乙酸乙酯部位浸膏中可能含有糖、多糖、苷类、皂苷、有机酸、黄酮类、酚类、香豆素、内酯、植物甾醇、三萜类等化学成分,为进一步研究壮药三叶香茶菜乙酸乙酯部位的化学成分和药理作用提供依据。 相似文献
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《Electronic Journal of Biotechnology》2014,17(5):193-198
BackgroundStrawberry is a pseudofruit mainly cultivated in temperate climate regions. Considering its high levels of ascorbic acid and phenolic compounds, the consumption of strawberry fruit can be beneficial to health. The Brazilian strawberry production revolves around 3000 tons per year, significantly influencing the food market and generating income to farmers. However, this production can be partially impaired by two-spotted spider mite (TSSM) Tetranychus urticae Koch infestations, due to decreases in the quality and quantity of fruit. Since there are no data in the literature about alterations caused by TSSM infestation in strawberry plants, our work aimed towards evaluating nutritional and physicochemical parameters of TSSM-infested strawberry plants, along with the related chemical treatment (CT) (acaricide) or biological treatment (predatory mite Phytoseiulus macropilis Banks).ResultsStrawberry fruit from TSSM-infested plants present the highest levels of acidity and exhibit low levels of anthocyanin and phenolic compounds, while fruit from TSSM-infested plants + biological control using predatory mite shows high levels of soluble solids, phenolic compounds and ascorbic acid, along with a high soluble solid content/titratable (SSC/TA) acidity ratio, which indicates high quality fruit.ConclusionsOur results suggest that TSSM infestation decreases fruit quality and that the biological control of TSSM using a predatory mite is a suitable alternative to organic production, since the presence of predatory mite does not affect fruit quality and development. 相似文献
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Santosh Kumar Maurya Kanwal Raj Arvind Kumar Srivastava 《Indian journal of clinical biochemistry : IJCB》2009,24(4):404-409
The root of Glycyrrhiza glabra is a traditional medicine used mainly for the treatment of peptic ulcer, hepatitis C, pulmonary
and skin diseases, although clinical and experimental studies suggest that it has several other useful pharmacological properties
such as antiinflammatory, antiviral, antimicrobial, antioxidative, anticancer activities, immunomodulatory, hepatoprotective
and cardioprotective effects. Glycyrrhizinic acid, a major component of licorice, has antiulcer effect by raising the local
concentration of prostaglandins that promote mucous secretion and cell proliferation in the stomach. Glycyrrhizin shows hepatoprotective
effect by preventing changes in cell membrane permeability, inhibiting phospholipase A2 (PLA2) and increasing survival rate of hepatocytes. Glabridin has effect in melanogenesis and inflammation by inhibiting the tyrosinase
activity of melanocytes. α-glycyhrritinic acid exhibits anti-inflammatory activity by inhibiting glucocorticoid metabolism.
In present study ethanolic (95%) extract of root of Glycyrrhiza glabra and its fractions were investigated for its antidyslipidaemic
activity on HFD induced dyslipidaemic hamsters. Ethanolic extract and its ethyl acetate soluble, water soluble and hexane
soluble fractions decreased serum level of total cholesterol by 25.9, 38.0, 39.0 and 26.3%, respectively. On the other hand
ethanolic extract, ethyl acetate soluble, water soluble and hexane soluble fraction increased the serum HDL-cholesterol level
by 14.8, 34.3, 27.3 and 17.2%, respectively. Ethanolic extract, ethyl acetate fraction, aqueous fraction and hexane fraction
decreased triglyceride level by 31.3, 37.2, 41.2 and 28.9%, respectively. The reduction in LDL-cholesterol level by ethanolic
extract, ethyl acetate soluble fraction and water soluble fraction were 43.9, 31.0, 33.4 and 24.6%, respectively. 相似文献
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中药寒热相关药性和抑制脂肪酸合酶能力关系的探索 总被引：1，自引：0，他引：1
测定了药典中106味温热性中药和119味寒凉性中药对脂肪酸合酶的抑制水平,发现温热药中对该酶强抑制的频数高于寒凉药约38％.进一步分析发现,温热药中的温里和散内寒药中强抑制药的频数大幅提高,而寒凉药中的清热泻火、明目、清虚热药中强抑制药的频数则进一步降低,差异均具有显著性.综合以上结果提出,直接影响能量代谢系统、造成能量代谢率变化是形成中药的寒热药性的因素之一. 相似文献
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In this work, we demonstrate the use of stereolithographic 3D printing to fabricate millifluidic devices, which are used to engineer particles with multiple compartments. As the 3D design is directly transferred to the actual prototype, this method accommodates 3D millimeter-scaled features that are difficult to achieve by either lithographic-based microfabrication or traditional macrofabrication techniques. We exploit this approach to produce millifluidic networks to deliver multiple fluidic components. By taking advantage of the laminar flow, the fluidic components can form liquid jets with distinct patterns, and each pattern has clear boundaries between the liquid phases. Afterwards, droplets with controlled size are fabricated by spraying the liquid jet in an electric field, and subsequently converted to particles after a solidification step. As a demonstration, we fabricate calcium alginate particles with structures of (1) slice-by-slice multiple lamellae, (2) concentric core-shells, and (3) petals surrounding the particle centers. Furthermore, distinct hybrid particles combining two or more of the above structures are also obtained. These compartmentalized particles impart spatially dependent functionalities and properties. To show their applicability, various ingredients, including fruit juices, drugs, and magnetic nanoparticles are encapsulated in the different compartments as proof-of-concepts for applications, including food, drug delivery, and bioassays. Our 3D printed electro-millifluidic approach represents a convenient and robust method to extend the range of structures of functional particles. 相似文献
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《Electronic Journal of Biotechnology》2014,17(6):251-261
BackgroundFatty acid synthase (FAS) is a key enzyme of de novo lipogenesis (DNL), which has been cloned from several species: Gallus gallus, Mus musculus, Homo sapiens, but not from Anas platyrhynchos. The current study was conducted to obtain the full-length coding sequence of Peking duck FAS and investigate its expression during adipocyte differentiation.ResultsWe have isolated a 7654 bp fragment from Peking duck adipocytes that corresponds to the FAS gene. The cloned fragment contains an open reading frame of 7545 bp, encodes a 2515 amino acid protein, and displays high nucleotide and amino acid homology to avian FAS orthologs. Twelve hour treatment of oleic acid significantly up-regulated the expression of FAS in duck preadipocytes (P < 0.05). However, 1000 μM treatment of oleic acid exhibited lipotoxic effect on cell viability (P < 0.05). In addition, during the first 24 h of duck adipocyte differentiation FAS was induced; however, after 24 h its expression level declined (P < 0.05).ConclusionWe have successfully cloned and characterized Peking duck FAS. FAS was induced during adipocyte differentiation and by oleic acid treatment. These findings suggest that Peking duck FAS plays a similar role to mammalian FAS during adipocyte differentiation. 相似文献
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田维熙 《中国科学院研究生院学报》1990,(2)
本文对六个修饰巯基的试剂失活脂肪酸合成酶(FAS)的动力学进行了系统研究,发现它们与FAS的作用是否属于亲和标记,修饰巯基时的专一性,以及盐和NADPH有无保护,均只与该试剂分子中有无羧基负离子有关,提出在FAS的缩合中心存在一个对羧负离子有特异亲和力的结合部位.该部位可能是丙二酰基在缩合中心的结合部位,为缩合反应所必需,它使携带了丙二酰基的活臂向缩合中心运动,并结合于此进行缩合反应. 相似文献
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G. R. Sajitha K. T. Augusti Regi Jose 《Indian journal of clinical biochemistry : IJCB》2016,31(3):260-269
Heavy metal pollution is a global public health challenge due to its stable and persistent environmental contamination. Of these lead is considered to be one of the most common ubiquitous and industrial pollutants and at low concentration it exerts extensive damages to the tissues. Daily feeding of lead acetate solution (Dose: 10 mg/kg/day) to normal rats for a month adversely altered the parameters of blood, serum and tissues, viz; RBC, WBC, Hb, ?- ALAD (Delta amino levulinic acid dehydratase), Pb content, lipids, oxidized lipids (TBARS), vitamins C and E and GSH levels and activities of AST, ALT and antioxidant enzymes viz; catalase, GR, Gpx and SOD. In order to study whether antioxidants have any effect to counteract the toxicity of lead we have selected comparatively better active allium fractions for the study viz: polar fraction of garlic (PFG) and polar fraction of onion (PFO). On feeding of these active fractions of garlic and onion oils i.e. their polar fractions and vitamin E (Dose 100 mg/kg/day) separately for a month along with or without lead acetate to rats each nutraceutical and vitamin E counteracted the adverse effects of Pb significantly (p ≤ 0.05). Their effects are in the order of PFG > PFO > Vitamin E. All these results point out that garlic and onion oils contain natural disulfoxide compounds which act as antioxidant and anti toxic to lead compounds. Their comparative differences in action may be due to the presence and position of double bonds and disulfide oxide bonds in their molecules. i.e., in PFG the allyl disulfide oxide group is present and in PFO saturated methyl and propyl groups and unsaturated propenyl group are present in place of allyl groups. The former group confers a better antioxidant activity on PFG, while the latter groups confer a lesser activity on PFO. 相似文献