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1.
To understand genetic patterns of the morphological and physiological traits in flag leaf of barley, a double haploid (DH) population derived from the parents Yerong and Franklin was used to determine quantitative trait loci (QTL) controlling length, width, length/width, and chlorophyll content of flag leaves. A total of 9 QTLs showing significantly additive effect were detected in 8 intervals on 5 chromosomes. The variation of individual QTL ranged from 1.9% to 20.2%. For chlorophyll content expressed as SPAD value, 4 QTLs were identified on chromosomes 2H, 3H and 6H; for leaf length and width, 2 QTLs located on chromosomes 5H and 7H, and 2 QTLs located on chromosome 5H were detected; and for length/width, 1 QTL was detected on chromosome 7H. The identification of these QTLs associated with the properties of flag leaf is useful for barley improvement in breeding programs. Project supported by the National Natural Science Foundation of China (No. 30630047) and the Project on Absorption of Intellects by Institutions of Higher Education for Academic Disciplinary Innovations (the 111 Project) (No. B06014), China  相似文献   

2.
High malting quality of barley (Hordeum vulgate L.) relies on many traits, such as β-amylase and limit dextrinase activities and β-glucan and protein fraction contents. In this study, interval mapping was utilized to detect quantitative trait loci (QTLs) affecting these malting quality parameters using a doubled haploid (DH) population from a cross of CM72 (six-rowed) by Gairdner (two-rowed) barley cultivars. A total of nine QTLs for eight traits were mapped to chromosomes 3H, 4H, 5H, and 7H. Five of the nine QTLs mapped to chromosome 3H, indicating a possible role of loci on chromosome 3H on malting quality. The phenotypic variation accounted by individual QTL ranged from 8.08% to 30.25%. The loci of QTLs for β-glucan and limit dex-trinase were identified on chromosomes 4H and 5H, respectively. QTL for hordeins was coincident with the region of silica eluate (SE) protein on 3HS, while QTLs for albumins, globulins, and total protein exhibited overlapping. One locus on chromosome 3H was found to be related to β-amylase, and two loci on chromosomes 5H and 7H were found to be associated with glutelins. The identification of these novel QTLs controlling malting quality may be useful for marker-assisted selection in improving barley malting quality.  相似文献   

3.
INTRODUCTION Aluminum (Al) toxicity is one of the mostimportant yield-limiting factors for crop grown onacid upland and lowland acid sulphate soils (IRRI,1978). Al toxicity results in a reduced and damagedroot system, which in turn causes the affectedplants to be susceptible to drought stress and min-eral nutrient deficiencies (Foy, 1988). The physio-logical and biochemical mechanisms of the toxiceffect of Al on root elongation had been extensivelyinvestigated (Matsumoto, 2000). T…  相似文献   

4.
Aluminum (Al) toxicity is the major factor limiting crop productivity in acid soils. In this study, a recombinant inbreed line (RIL) population derived from a cross between an A1 sensitive lowland indica rice variety IR1552 and an Al tolerant upland japonica rice variety Azucena, was used for mapping quantitative trait loci (QTLs) for A1 tolerance. Three QTLs for relative root length (RRL) were detected on chromosome 1, 9, 12, respectively, and I QTL for root length under Al stress is identical on chromosome I after one week and two weeks stress. Comparison of QTLs on chromosome 1 from different studies indicated an identical interval between C86 and RZ801 with gene(s) for Al tolerance. This interval provides an important start point for isolating genes responsible for A1 tolerance and understanding the genetic nature of Al tolerance in rice. Four Al induced ESTs located in this interval were screened by reverse Northern analysis and confirmed by Northern analysis. They would be candidate genes for the QTL.  相似文献   

5.
The phosphorus uptake (PU) in above-ground parts of plant, root characteristics and root exudations as well as the quantitative trait loci (QTLs) associated with these characteristics were determined for a F2:3 population derived from the cross of two contrasting maize (Zea mays L.) genotypes, 082 and Yel07. A total of 241 F2:3 families were evaluated in replicated trials under deficient phosphorus conditions in 2007 at two sites (Kaixian County and Southwest University, Chongqing, P. R. China). The results show pleiotropy and close linkage among QTLs. Four common regions in different environments were in bnlg100- bnlg1268b (bins 1.02) for QTL of H+, bnlg1268a-umc1290a (bins 1.09) for QTL of AP (acid phospbatase activity), dupssrl5- P 1MT/a (bins 6.06) for QTLs of PU (phosphorus uptake) and RW (root weight), and P IM3/d-P1M3/g (bins9.04) for QTLs of PU and AP. These QTLs are non-environment or minor QTLs x environment. By epistatic analysis, three main QTLs and eighteen QTLs x QTLs interactions were detected for the seven measured characteristics. These QTLs may affect trait expression by epistatic interaction with the other loci, and make a substantial contribution to phosphorus utilization efficiency, which should be considered when breeding maize varieties with high P efficiency. Two regions were detected in dupssrl 5- P1MT/a (bins 6.06) for QTL of RW and P1M3/d- P 1M3/g (bins 9.04) for QTL of PU and AP. They were detected in two different environments and by two methods of QTL analysis, which were useful for marker-assisted selection.  相似文献   

6.
Quantitative trait loci (QTL) analysis was conducted in bread wheat for 14 important traits utilizing data from four different mapping populations involving different approaches of QTL analysis. Analysis for grain protein content (GPC) sug- gested that the major part of genetic variation for this trait is due to environmental interactions. In contrast, pre-harvest sprouting tolerance (PHST) was controlled mainly by main effect QTL (M-QTL) with very little genetic variation due to environmental interactions; a major QTL for PHST was detected on chromosome arm 3AL. For grain weight, one QTL each was detected on chromosome arms 1AS, 2BS and 7AS. QTL for 4 growth related traits taken together detected by different methods ranged from 37 to 40; nine QTL that were detected by single-locus as well as two-locus analyses were all M-QTL. Similarly, single-locus and two-locus QTL analyses for seven yield and yield contributing traits in two populations respectively allowed detection of 25 and 50 QTL by composite interval mapping (CIM), 16 and 25 QTL by multiple-trait composite interval mapping (MCIM) and 38 and 37 QTL by two-locus analyses. These studies should prove useful in QTL cloning and wheat improvement through marker aided selection.  相似文献   

7.
采用土表接种的方法,对145份适合于淮河流域种植的小麦品种(系)进行了赤霉病的抗性鉴定和评价。以病小穗率为指标,与株高、穗长、旗叶长和旗叶宽等农艺性状做了相关性分析。结果表明,能够直接应用于大田生产的赤霉病抗性品种比较匮乏,145份材料中没有发现达到免疫和抗病水平的品系,2份达到中抗水平,46份材料表现为中感,10份材料介于中感至感病之间,87份材料表现为感病。从材料地理来源上看,仅有来自江苏的种质存在较好的抗病基因资源,而山东、安徽和河南地区的小麦品系普遍缺乏抗病性。相关性分析显示,病小穗率与株高极显著负相关,与旗叶长和旗叶宽极显著正相关,感病性的降低和丰产性的提高存在矛盾。  相似文献   

8.
安陆白花菜是湖北地方性经济作物,市场价值和药用价值都较高,本研究采用荧光原位杂交技术,对5SrDNA和45SrDNA在安陆白花菜有丝分裂中期染色体上的物理位置进行了定位分析。结果显示5SrDNA杂交信号l对,位于第11号染色体上长臂;45S杂交信号1对,位于第16号染色体上长臂。安陆白花菜的染色体数目为34条,染色体基数17,2A核型,核型公式为K(2n)=34=14m(2SAT)+20sm(2SAT),其中第11、16号染色体长臂末端带有随体,平均臂比1.733,最长染色体/最短染色体1.478,臂比大于2的比率29.41,核型不对称系数62.73,染色体相对长度组成为2L+12M2+20M1。  相似文献   

9.
Immortalized F2 population of rice (Oryza sativa L.) was developed by randomly mating F1 among recombinant inbred (RI) lines derived from (Zhenshan 97B ( Minghui 63), which allowed replications within and across environments. QTL (quantitative trait loci) mapping analysis on kilo-grain weight of immortalized F2 population was performed by using newly developed software for QTL mapping, QTLMapper 2.0. Eleven distinctly digenic epistatic loci included a total of 15 QTL were located on eight chromosomes. QTL main effects of additive, dominance, and additive ( additive, additive ( dominance, and dominance ( dominance interactions were estimated. Interaction effects between QTL main effects and environments (QE) were predicted. Less than 40% of single effects, most of which were additive effects, for identified QTL were significant at 5% level. The directional difference for QTL main effects suggested that these QTL were distributed in parents in the repulsion phase. This should make it feasible to improve kilo-grain weight of both parents by selecting appropriate new recombinants. Only few of the QE interaction effects were significant. Application prospect for QTL mapping achievements in genetic breeding was discussed.  相似文献   

10.
以玉米为材料,研究氮磷钾不同运筹模式对其生理特性、品质及产量性状的影响。结果表明,氮磷钾不同运筹方式对玉米穗位叶叶绿素含量、硝酸还原酶活性、穗长、穗粗、品质以及产量都有影响,且在不同施肥水平下,不同氮钾基追肥比例对性状的影响不同。在施肥水平高的条件下,氮钾基追肥比例以7/3产量最高;在施肥水平中等的条件下,氮钾基追肥比例以6/4玉米产量最高;在施肥水平低的条件下,氮钾基追肥比例以5/5玉米产量最高。  相似文献   

11.
300 mmol/L NaCl明显抑制大麦幼苗的生长, NaCl处理的同时加入 20 mg/L的 GSH可明显增加大麦幼苗干重、根系长度、叶片叶绿素以及K+含量,降低Na+含量;同时叶片液泡膜H+-ATPase和H+-PPase的活性均明显增加,电解质渗漏率降低.  相似文献   

12.
赤霉素对杂交水稻生育后期氮代谢的调控作用   总被引:1,自引:0,他引:1  
杂交水稻(Oryza sativa subsp indica)农艺性状优良,表现了强大的增产优势。杂交水稻的氮代谢与其农艺性状密切相关,然而关于杂交水稻的氮代谢及氮代谢调控的研究较少。赤霉素对植物有着广泛影响,在杂交水稻生育后期用适当浓度的赤霉酸(GA_3)溶液处理,对其同化物的积累和转运及活性氧清除剂的活力等方面都有明显的促进作用(曾富华、罗泽民,1991、1993)。本试验以硝酸还原酶活力(NRA)、谷—丙转氨酶活力(GPTA)等作指标,选用威优35(V35,后健组合)和威优49(V49,早衰组合)为材料,大田小区裁  相似文献   

13.
300 mmol/L NaCl明显抑制大麦幼苗的生长,NaCl处理的同时加入20 m妙L的GSH可明显增加大麦幼苗干重、根系长度、叶 片叶绿素以及K+含量,降低Na+含量;同时叶片液泡膜H+-ATPase和H+-PPase的活性均明显增加,电解质渗漏率降低.  相似文献   

14.
INTRODUCTIONKilo-grainweight,characteristicallyacom-plextrait,isanimportantcomponentofyieldinrice.QTLmappingforkilo-grainweightofricewascon-ductedusingpopulationsofF2(Linetal.,1996),doubledhaploidlines(Luetal.,1997),recombi-nantinbredlines(Xiaoetal.,1996),backcrosstestcross(Xiaoetal.,1995;Lietal.,1997)andratoonedF2(Lietal.,2000).Consideringthesen-sitivityofkilo-grainweighttoenvironments,Luetal.(1997)andZhuangetal.(1997)comparedthedifferentialdetectionofQTLacrossenvironmentstodetermin…  相似文献   

15.
Seed vigor is an important characteristic of seed quality, and rice cultivars with strong seed vigor are desirable in direct-sowing rice production for optimum stand establishment. In the present study, the quantitative trait loci (QTLs) of three traits for rice seed vigor during the germination stage, including germination rate, final germination percentage, and germination index, were investigated using one recombinant inbred line (RIL) population derived from a cross between japonica Daguandao and indica IR28, and using the multiple interval mapping (MIM) approach. The results show that indica rice presented stronger seed vigor during the germination stage than japonica rice. A total of ten QTLs, and at least five novel alleles, were detected to control rice seed vigor, and the amount of variation (R 2) explained by an individual QTL ranged from 7.5% to 68.5%, with three major QTLs with R 2>20%. Most of the QTLs detected here are likely to coincide with QTLs for seed weight, seed size, or seed dormancy, suggesting that the rice seed vigor might be correlated with seed weight, seed size, and seed dormancy. At least five QTLs are novel alleles with no previous reports of seed vigor genes in rice, and those major or minor QTLs could be used to significantly improve the seed vigor by marker-assisted selection (MAS) in rice.  相似文献   

16.
以农大108玉米为材料,在苗期进行缺氯处理,结果如下:(1)叶片长度在处理后第5天出现明显差异,处理组叶长低于对照组,仅为对照组的66.67%;(2)对照与处理组间叶绿素含量从第6天开始出现显著差异;(3)处理组叶片蛋白质含量在处理后第5天明显低于对照组;(4)叶片硝酸还原酶活性在处理的第4天明显低于对照组,并始终低于对照组水平.综合利用上述指标可以在出苗后4-6d时间内对缺氮玉米幼苗做出比较准确的诊断.  相似文献   

17.
研究了地下水水位为2 m时,不同浓度的咸水(1.5、2.5、3.5和4.5 g/L)灌溉对冬小麦株高、叶面积指数、分蘖干重、叶绿素含量的影响.结果表明:当咸水的浓度为1.5 g/L时,平均株高为58.9 cm,平均叶面积指数为5.69,平均分蘖干重为1.41 g,平均叶绿素含量为26.39 mg/L;与1.5 g/L浓度咸水灌溉相比,浓度为2.5 g/L时平均株高、平均叶面积指数、平均分蘖干重和平均叶绿素含量分别减少1.8%、10.5%、10.6%和1.7%;灌溉咸水浓度为3.5 g/L时平均株高、平均叶面积指数、平均分蘖干重和平均叶绿素含量较1.5 g/L时分别减少4.6%、22.8%、22.0%和3.6%;灌溉咸水浓度为4.5 g/L时平均株高、平均叶面积指数、平均分蘖干重和平均叶绿素含量较1.5 g/L时分别减少8.5%、29.0%、27.6%和5.6%.灌溉咸水浓度越高,对冬小麦生长的抑制作用越大.  相似文献   

18.
以青稞幼苗为试材,采用营养液水培法研究了200mmol/L NaCl胁迫对青稞幼苗叶片相对含水量(RWC)、叶绿素、丙二醛(MDA)、脯氨酸含量的影响.结果显示,在200mmol/L NaCl胁迫2,4,6,8d后,青稞幼苗叶片相对含水量、叶绿素含量随胁迫的进行而不断下降,其中叶绿素含量在前4d下降尤其明显;MDA和脯氨酸含量则显著上升.表明200mmol/L NaCl胁迫对青稞幼苗造成了严重的生理伤害.  相似文献   

19.
实验选择60盆盆栽月季,随机分成处理1组、处理2组、处理3组和对照组,每组5盆,重复3次,研究KH2PO4不同用量对盆栽月季的影响.结果表明KH2PO4用量适宜时,对月季新梢的茎长和茎粗有明显的促进作用,可增加叶片干重和叶绿素含量,并可提高花色等级、增加花产量,其中KH2PO4的最佳用量是6g/盆.  相似文献   

20.
在杂交水稻汕优63始稳期,用15mmol/LCa(2 =)喷施叶面,能增加抽穗结实期间主茎旗叶的叶绿素和可溶性蛋白质含量,提高超氧物歧化酶(SOD)的活性.降低丙二醛(MDA)含量.延缓叶片衰老;同时,Ca(2 )能增加主德的实位数、结实串和千粒重,从而提高谷产量.  相似文献   

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