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1.
通过筛选从土壤和腐朽树木中得到了4株纤维素酶高产菌株MF1、MF4、TF3和TF9,经初步鉴定分别是绿色木霉、青霉属、康氏木霉和黑曲霉.通过对TF3发酵产酶的影响因素研究表明,其发酵产酶的最佳碳源是麸皮-纤维素粉复合物,最佳氮源是硫酸铵,最适发酵温度范围是30～32℃,最适初始pH范围是6～7. 相似文献
2.
通过筛选从土壤和腐朽树木中得到了4株纤维素酶高产菌株MF1、MF4、TF3和TF9,经初步鉴定分别是绿色木霉、扩展青霉、康氏木霉和黑曲霉.通过对TF3发酵产酶的影响因素研究表明,其发酵产酶的最佳碳源是麸皮-纤维素粉复合物,最佳氮源是硫酸铵,最适发酵温度范围是30～32℃,最适宜初始pH范围是6～7. 相似文献
3.
南韩灵芝母种培养基的筛选研究 总被引：1，自引：0，他引：1
通过对南韩灵芝菌株在不同碳源、氮源及不同配方的母种培养基的生长实验,筛选出适合其生长的碳源、氮源和最佳培养基配方．试验结果表明：南韩灵芝菌丝体在麸皮、酵母膏,蛋白胨3种氮源中,以麸皮为最适氮源;在葡萄糖、蔗糖和玉米粉3种碳源中,以玉米粉为最适碳源．在5种不同的培养基试验中,加富PDA培养基是最适合南韩灵芝生长的培养基配方． 相似文献
4.
为了提高微生物蛋白酶的发酵水平,从豆豉中筛选蛋白酶高产菌株并研究其产酶条件。采用平板透明圈法和摇瓶发酵法进行筛选,获得一株蛋白酶高产菌株DC17。通过菌体形态观察、生理生化特征分析和16S rDNA鉴定,菌种DC17被鉴定为解淀粉芽孢杆菌。利用小型发酵罐对菌株DC17进行蛋白酶发酵研究,确定了最佳的碳源、氮源、温度、pH及时间分别是可溶解性淀粉、牛肉膏、30℃、7.0和36 h。在最佳条件下,发酵液中的蛋白酶活力可达950 U/mL。 相似文献
5.
不同杏鲍菇菌株营养特性的研究 总被引：1，自引：0，他引：1
武模戈 《濮阳职业技术学院学报》2010,23(5)
本试验研究了不同杏鲍菇菌株的营养特性,经LSD法分析得出:不同杏鲍菇菌养Pe1的最佳碳氮源分别是葡萄糖和酵母粉;培养Pe2的最佳碳氮源分别是果糖和酵母粉;培养Pe5的最佳碳氮源分别是可溶性淀粉和酵母粉.羧甲基纤维素纳、柠檬酸钠不宜作为Pe1、Pe2、Pe5的碳源,而亚硝酸钠、硝酸钠、尿素不宜作为培养Pe1、Pe2、Pe5的氮源. 相似文献
6.
碳源、氮源及其比例对香菇液体深层培养的影响 总被引：1，自引：0，他引：1
通过摇瓶培养,研究香菇液体深层培养最适发酵培养时间、最适碳源、最适氮源及最佳碳源与氮源质量配比.结果表明最适发酵培养时间为168 h,所得菌丝体干重达0.399 g/50 mL菌液,最适碳源为蔗糖,菌丝体干重达到0.396 g/50 mL菌液;最适氮源为牛肉膏,菌丝干重达到0.421 g/50 mL菌液,并通过正交实验筛选出最佳碳源与氮源质量配比为:W(蔗糖):W(牛肉膏):W(麦芽糖)=2.5:1:1. 相似文献
7.
通过筛选从土壤和腐朽树木中得到了4株纤维素酶高产菌株MF1、MF4、TF3和TF9,经初步鉴定分别是绿色木霉、青霉属、康氏木霉和黑曲霉。通过对TF3发酵产酶的影响因素研究表明,其发酵产酶的最佳碳源是麸皮-纤维素粉复合物,最佳氮源是硫酸铵,最适发酵温度范围是30～32℃,最适初始pH范围是6～7。 相似文献
8.
通过三株红假单胞菌在不同碳源、氮源、pH值和不同温度等条件的混菌培养实验，筛选出适合其生长的最适碳源、氮源、pn值和培养温度、试验结果表明：该混合菌株在乙醇、丙酸钠、乙酸钠等5种碳源中，以乙酸钠为最适碳源；在氯化铵、硫酸铵等5种无机氮源中，以硫酸铵为最适氮；最适pH为8．5；最适培养温度为38℃。 相似文献
9.
几种栽培灵芝菌丝体生长营养条件之初探 总被引：1，自引：0，他引：1
通过三种栽培灵芝品种菌株在不同碳源、氮源及不同种配方的母种培养基上的生长实验，筛选出适合其生长的最适碳源、氮源和最佳培养基配方，试验结果表明：三种栽培灵芝菌株菌丝体在玉米粉、可溶性淀粉、蔗糖、葡萄糖、乳糖和走牙糖这几种碳源中，以玉米粉为最适碳源；在花生饼粉、酵母膏、蛋白胨等几种氮源中，以花生饼粉为最适氮源，并表明在PDA培养基中添加蛋白胨和大量元素对菌丝体生长均有促进作用，且其影响效果可以累加。 相似文献
10.
11.
辣椒炭疽病原菌生物学特性研究 总被引：1，自引：0，他引：1
对炭疽病典型症状的辣椒果实进行分离纯化获得炭疽病原菌,诱导产孢进行形态学鉴定,在不同温度、光照、培养基成分等条件下对该病原菌生物学特性进行研究。结果表明,诱导产孢进行形态学鉴定该病原菌为胶孢炭疽菌(Colletotrichum gloeosporioides);菌丝生长的最适温度为25~30℃;菌丝正常生长不受光照条件影响;菌丝的生长受大蒜和芥菜中的某些成份的抑制。该研究以期为辣椒病害防治以及品质的提高提供理论依据。 相似文献
12.
Young Soo Kim Kotnala Balaraju Yongho Jeon 《Journal of Zhejiang University. Science. B》2016,17(12):931-940
In this study, plant growth-promoting rhizobacteria (PGPR) were evaluated as potential biocontrol agents against postharvest pathogens of apple fruits. In vitro bioassays revealed that, out of 30 isolates screened, isolates APEC136 and APEC170 had the most significant inhibitory effects against the mycelial growth of several fungal pathogens. Analysis of 16S ribosomal RNA (rRNA) sequences identified the two effective isolates as Paenibacillus polymyxa and Bacillus subtilis, respectively. The two strains showed greater growth in brain-heart infusion broth than in other growth media. Treatment of harvested apples with suspensions of either strain reduced the symptoms of anthracnose disease caused by two fungal pathogens, Colletotrichum gloeosporioides and Colletotrichum acutatum, and white rot disease caused by Botryosphaeria dothidea. Increased productions of amylase and protease by APEC136, and increased productions of chitinase, amylase, and protease by APEC170 might have been responsible for inhibiting mycelial growth. The isolates caused a greater reduction in the growth of white rot than of anthracnose. These results indicate that the isolates APEC136 and APEC170 are promising agents for the biocontrol of anthracnose and white rot diseases in apples after harvest, and suggest that these isolates may be useful in controlling these diseases under field conditions. 相似文献
13.
Liu XIE Jing-ze ZHANG Yao WAN Deng-wei HU 《Journal of Zhejiang University. Science. B》2010,11(1):61-70
Strawberry anthracnose, caused by Colletotrichum spp., is a major disease of cultivated strawberry. This study identifies 31 isolates of Colletotrichum spp. which cause strawberry anthracnose in Zhejiang Province and Shanghai City, China. Eleven isolates were identified as C. acutatum, 10 as C. gloeosporioides and 10 as C. fragariae based on morphological characteristics, phylogenetic and sequence analyses. Species-specific polymerase chain reaction (PCR) and enzyme digestion further confirmed the identific... 相似文献
14.
Po Po THAN Haryudian PRIHASTUTI Sitthisack PHOULIVONG Paul W.J. TAYLOR Kevin D. HYDE 《Journal of Zhejiang University. Science. B》2008,9(10)
Anthracnose disease is one of the major economic constraints to chilli production worldwide, especially in tropical and subtropical regions. Accurate taxonomic information is necessary for effective disease control management. In the Colletotrichum patho-system, different Colletotrichum species can be associated with anthracnose of the same host. Little information is known concerning the interactions of the species associated with the chilli anthracnose although several Colletotrichum species have been reported as causal agents of chilli anthracnose disease worldwide. The ambiguous taxonomic status of Colletotrichum species has resulted in inaccurate identification which may cause practical problems in plant breeding and disease management. Although the management and control of anthracnose disease are still being extensively researched, commercial eultivars of Capsicum annuum that are resistant to the pathogens that cause chilli anthracnose have not yet been developed. This paper reviews the causal agents of chilli anthracnose, the disease cycle, conventional methods in identification of the pathogen and molecular approaches that have been used for the identification of Colletotrichum species. Pathogenetic variation and population structure of the causal agents of chilli anthracnose along with the current taxonomic status of Colletotrichum species are discussed. Future developments leading to the disease management strategies are suggested. 相似文献
15.
以饲料为原料筛选出一株既产乳酸又产芽孢的益生菌,经过分子生物学鉴定为凝结芽孢杆菌(Bacillus coagulans),命名为T-8。从培养基碳源、氮源、有机物浸出液、培养基pH、培养温度、培养时间等方面对菌株T-8展开发酵特性研究,结果表明,最优培养基配方为:玉米粉6 g/L、豆粕粉15 g/L、小麦麸皮浸出液60%、酵母粉5 g/L、蛋白胨10 g/L、氯化钠10 g/L,pH 5.0。以5%的接种量将种子液转接至最优培养基,在47℃的摇床中以200 r/min的转速振荡培养28 h,菌株T-8的细胞密度达到4.8×109 CFU/mL,芽孢率达到95.2%。本试验可为益生菌凝结芽孢杆菌应用于饲用微生物菌剂的工业化生产提供参考。 相似文献
16.
自盘锦油田低温环境土样中分离到1株低温菌,该菌能以石油烃为唯一碳源和能源。经形态学、生理生化和16S rDNA序列鉴定：菌株LHB16为嗜麦芽窄食单胞菌（Stenotrophomonas maltophilia）。紫外分光光度法测定该菌在含石油0.5%（w/v）的无机盐培养基中,20℃,振荡培养10 d,降解率达80.16%。经传代培养数代,降解性能稳定。GC-MS分析表明烷烃C15～C32被完全降解。菌株LHB16在低温地区石油污染的生物治理中有良好的应用前景。 相似文献
17.
以（NH4）2SO4为唯一氮源,经富集培养、平板涂布分离及平板划线纯化、摇瓶复筛等方法,从当地养殖池水体中分离、筛选氨氮降解菌。根据形态学、生理生化特征分析,对其进行归属鉴定,并研究其降解特性。通过对初步鉴定归属为沙雷氏菌属（Serratia．sp）,命名为N-2的氨氮降解菌降解特性的研究,得到其最适降解条件：N-2利用（NH4）：S04作唯一氮源时,最适碳源为蔗糖,降解温度为32—37℃,pH为7．0。在最适条件下,当NH4＋-N初始质量浓度为50mg／L时,该菌培养至72h氨氮降解率可达97．4％。结果表明,该菌为高效氨氮降解菌,这种土著微生物在修复富营养化水体方面有较好的应用前景。 相似文献
18.
Than PP Prihastuti H Phoulivong S Taylor PW Hyde KD 《Journal of Zhejiang University. Science. B》2008,9(10):764-778
Anthracnose disease is one of the major economic constraints to chilli production worldwide, especially in tropical and subtropical
regions. Accurate taxonomic information is necessary for effective disease control management. In the Colletotrichum patho-system, different Colletotrichum species can be associated with anthracnose of the same host. Little information is known concerning the interactions of the
species associated with the chilli anthracnose although several Colletotrichum species have been reported as causal agents of chilli anthracnose disease worldwide. The ambiguous taxonomic status of Colletotrichum species has resulted in inaccurate identification which may cause practical problems in plant breeding and disease management.
Although the management and control of anthracnose disease are still being extensively researched, commercial cultivars of
Capsicum annuum that are resistant to the pathogens that cause chilli anthracnose have not yet been developed. This paper reviews the causal
agents of chilli anthracnose, the disease cycle, conventional methods in identification of the pathogen and molecular approaches
that have been used for the identification of Colletotrichum species. Pathogenetic variation and population structure of the causal agents of chilli anthracnose along with the current
taxonomic status of Colletotrichum species are discussed. Future developments leading to the disease management strategies are suggested.
相似文献