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1.
White-spot disease of Chinese soft-shelled turtles (Trionyx sinens) caused by Paecilomyces lilacinus
Chinese soft-shelled turtles (Trionyx sinens) in culture farms using an artificial warming system in Zhejiang, China, often show typical signs of white-spot disease such as white spots on their bodies, skin lesions, anorexia and eventually death. The sick turtles were mostly 5~80 g in weight. A suspected fungal pathogen was isolated from the sick turtles and verified as Paecilomyces lilacinus by sequence analysis of the internal transcribed spacer (ITS) of its ribosomal DNA (rDNA). Detailed morphological examinations were also conducted to confirm the white-spot disease. 相似文献
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Kartik Shanker 《Resonance》1999,4(7):68-78
Ridleys are unique amongst sea turtles in exhibiting massnesting, when several thousand turtles migrate en masse to their nesting ground to mate and nest simultaneously. Gahirmatha, in Orissa, has the largest sea turtle rookery in the world, with 100,000 to 500,000 turtles nesting there in most years. Mass mortality and fragmentation of the beach led to the failure of the arribada in 1997 and 1998, but more than 200,000 turtles nested on the 2 km long island in Gahirmatha in March, 1999. Despite the occurrence of the arribada, the outlook for the turtles is.bleak since 10000 turtles were drowned as incidental catch in shrimp trawling operations. Over 40,000 turtles have been washed ashore dead since 1994 as a result of drowning in trawl fishing nets. The implementation of existing laws preventing mechanised fishing in near-shore waters and the mandatory use of turtle excluder devices on trawlers would substantially reduce mortality. The consolidation of current conservation efforts is necessary to ensure that this species does not disappear from Indian waters in the near future 相似文献
3.
Lyu Sunjian Yuan Xuemei Liu Li Zhang Haiqi Yu Zhe Hang Xiaoying Shi Weida Wu Yinglei 《Journal of Zhejiang University. Science. B》2021,22(4):295-304
Trionyx sinensis Hemorrhagic Syndrome Virus(TSHSV) is an arterivirus newly discovered in Chinese softshell turtles. Little is known about the effect of antibodies against the virus or the distribution of the virus in different organs of infected turtles. In this study, a partial protein of TSHSV-HP4 was produced using a prokaryotic expression system, and its polyclonal antibody was generated. The polyclonal antibody was confirmed by western blot and dot enzyme-linked immunosorbent assay(dot-ELISA). The distribution of TSHSV in different organs of T. sinensis was examined by immunohistochemistry(IHC) and the expression of immune-related genes was analyzed using quantitative real-time polymerase chain reaction(qRT-PCR). The results indicated that the recombinant TSHSV-HP4 protein was successfully expressed, and the generated polyclonal antibody showed specific binding to viral particles in the lung tissues of infected turtles. The IHC assay indicated that the virus was highly localized in various cells, including intestinal lymphocytes,enterocytes, kidney epithelial cells, spleen cells, lung macrophages, and cardiomyocytes. The qRT-PCR analysis revealed that TSHSV was detected in all organs tested, including the lungs, liver, kidneys, spleen, and heart. The numbers of viral mRNA copies in lung and heart tissues were significantly higher in the virus-antibody group than in the virus group. The interferonstimulated genes(ISGs), myxovirus resistance protein 2(MX2) and radical S-adenosyl methionine domain containing 2(RSAD2) were highly upregulated in all groups of infected turtles. Antibody-dependent enhancement(ADE) seemed to occur after stimulation by the polyclonal antibody, because significantly greater expression of the two genes was detected in the virus-antibody group than in the virus group. Overall, these results are important in understanding the cell localization of TSHSV and the immune response of infected turtles. 相似文献
4.
一起猪链球菌病的诊断及防治 总被引：1，自引：0，他引：1
本文报道了2004年7月,黄山市部分地区猪群暴发了一起急性败血性传染病。发病率及死亡率均高。根据流行病学,临床表现以及细菌分离培养,诊断此次流行的疾病系猪急性败血型链球菌病,药敏实验及临床治疗表明,本次分离到的链球菌对氨苄青霉素、克林霉素敏感。而对常规青霉素低敏。 相似文献
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程喜荣 《黄冈职业技术学院学报》2002,4(4):59-63
本实验对某鸡场鸡群中出现的一种以呼吸道症状和产蛋下降为主要特征的疫情进行了临床症状和流行病学调查,对病死鸡进行了解剖检查并从病鸡病料中分离到一种病毒,通过血凝(HA)和血凝抑制(HI)试验、病毒中和试验(VNT)和琼脂免疫扩散试验(AGP)等方法对该病毒进行了签字。结果表明该次疫情为A型流感病毒感染。 相似文献
7.
This study examined students alternative conceptions of reptiles and amphibians and the extent to which these conceptions remain intact through the elementary (grades 4 and 6), junior, and senior high school years. We administered multiple-choice and free-response instruments to a total of 513 students and interviewed at least 20 students at each educational level to get an in-depth view of their original conceptions. Then, we developed and administered a two-tier multiple choice diagnostic instrumentto assess various levels of students understanding of amphibians and reptiles(N=1267). The results showed that most students were able to classify snakes as reptiles, whereas fewer than 30% of the students across different ages classified sea turtles as reptiles; the remaining 70% classified sea turtles as amphibians. More students were able to correctly classify frogs as amphibians than toads. In most instances, students correctly classified prototypical representatives of the two animal classes more readily than less exemplary representatives, a finding that supports previous research (Trowbridge, J.E. andMintzes, J.J. (1988). Alternative conceptions in animal classification: A cross-age study. Journal of Research in Science Teaching, 25(7), 547–571). Interestingly, the alternative views of sea turtles as amphibians remained intact throughout the school years. Interview data indicated that students classified sea turtles as amphibians largely because sea turtles are able to live in terrestrial and aquatic habitats. Semantically, amphi means living on land and in water. When asked to distinguish between reptiles and amphibians and to classify several species into those two groups, a wide range of alternative conceptions emerged and the origin of those alternative conceptions are discussed. Similar results were obtained when we applied a two-tier multiple-choice diagnostic instrument to assess students understanding of amphibians and reptiles. 相似文献
8.
The relationship between neonatal characteristics and three-month mother-infant interaction in high-risk infants 总被引：1，自引：0，他引：1
The behavior of 4 groups of infants--healthy term, healthy preterm, sick preterm, and sick full-term--was assessed in the neonatal period using the Neonatal Behavioral Assessment Scale (NBAS). At 3 months postterm, infants and their mothers were observed and videotaped in a free-play session. Both the NBAS and mother-infant interaction data were analyzed to assess the effects of maturity (term vs. preterm), illness (sick vs. healthy), and their interaction. Results revealed that illness of the infant affected both NBAS performance and maternal behavior during the interaction at 3 moths. Infants who were ill performed poorly on the NBAS orientation dimension; this dimension was found to be significantly associated with maternal and infant behaviors at 3 months. These data demonstrate an association between early infant characteristics and subsequent mother and child interactive behaviors. They also identify postnatal illness as an important influence on the development of the mother-infant dyad. 相似文献
9.
Lei SJ 《Journal of Zhejiang University. Science. B》2006,7(7):580-585
The effects of ration level and feeding frequency on digestibility in juvenile soft-shelled turtle, Pelodiscus sinensis, were investigated. Four ration levels 1.5%, 2.5%, 4.0% and satiation （6.0% BW/d） were used. Apparent digestibility （AD） of dry matter （DMAD）, protein （PAD） and protein real digestibility （PRD） were significantly affected by ration level, but not by feeding frequency when the ration level was similar. However, the feeding frequency affected the AD, DMAD, PAD and PRD significantly when the turtles were fed to satiation. The relationship between fecal protein content （Y） and protein intake （At） can be expressed as a quadric equation： Y=-0.1742＋0.1476X-0.0003X^2 （r^2=0.876, n=27, F=93.92, P〈0.01）. 相似文献
10.
This study examined physician‐patient communication as it relates to expectations that patients and physicians hold for themselves and each other. Dimensions of mutual role expectation were determined to be physician as humanistic practitioner, physician authority, patient in the sick role, and patient as consumer. The study tested five hypotheses. Results showed patients had significantly higher expectations for patients in the sick role than had physicians. Other role expectations varied significantly by age within physician and patient groups. The implications of these differences for physician‐patient communication are discussed. 相似文献
11.
Kartik Shanker 《Resonance》2002,7(6):53-66
Marine turtles are known to migrate several thousands of kilometres between their feeding and breeding grounds. These migrations have been studied by the application of metal tags on flippers, with intense monitoring at nesting sites, and opportunistic recovery in offshore waters, providing information on turtle movements. Often, several thousands of tags have been applied with very low levels of recapture. Satellite telemetry is a high-tech, and expensive, method to track turtles during their migration across the open ocean. Molecular genetic techniques have offered ways to track turtles both through space and time. The comparison of genetic haplotypes from different regions makes it possible to study population structure, test theories of natal homing, and even assign feeding populations to nesting sites and vice versa. The study of these haplotypes has also made it possible to look at relationships between species and populations, and study evolutionary biogeography or phylogeography of these animals. 相似文献
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Six desert iguanas (Dipsosaurus dorsalis) were trained on a black-white discrimination problem using a modified Lashley maze I. The results, in terms of number of trials to criterion, compared favorably to those reported for turtles on the same brightness difference. Efficiency of learning was attributed to (1) the angle separating the discriminanda, (2) intertrial substrate temperature, and (3) visual attentional characteristics of the apparatus. In addition, orienting responses (VTE) varied in expected directions according to speed of learning and difficulty of maze choice points. 相似文献
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王晓苏 《泰州职业技术学院学报》2013,(3):72-74
目的探讨子宫肌瘤围手术期实施舒适护理措施。方法对102例子宫肌瘤患者分别在术前、术中、术后实施舒适护理措施。结果所有患者经过精心治疗和护理,术后均痊愈出院。结论舒适护理作为整体护理的延伸和拓展,注重“以人为本”的护理理念,从患者的角度来考虑患病过程中的心理、家庭和社会因素对患者的治疗和康复的影响,实施护理措施,可以有效减轻患者的病程,有利于提高患者术后的生活质量。 相似文献
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姚金玲 《南宁师范高等专科学校学报》2004,21(3):35-37
本文论述了乡愁的表现形式,指出乡愁中的“乡”寄寓的是作者的理想之乡,乡愁是作者的精神寄托,借助弗洛姆、马斯洛的理论,本文还从心理方面阐述了乡愁的深层内蕴。 相似文献
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姜英辉 《湖北广播电视大学学报》2008,28(11):77-78
秦汉时期是中国的交通条件得到重要发展的时期,然而秦汉时人的远行依然充满艰辛和危险,这可以从史籍经常见到的游人“行道病”“道病卒”的记载中得到证明。究其原因,依然是受到当时的交通工具、气候条件等因素制约的结果。 相似文献
17.
邓希雯 《内江师范学院学报》2013,(11):90-94
中国龟文化发源于上古神话.在中华文明的发源地,先民发现了龟的神奇能力,在龟身上寄托了对生命的崇拜.经过各朝代的发展演变,龟文化经历了从辉煌到衰退.在这一演变过程中,龟文化包含了丰富的符号学意义,即能指：堙堵、自保、防御、退缩、缓慢、智慧、神秘、力量、生殖崇拜十项;所指：和谐的自然精神、顽强的生命意识、穿越的宇宙意义三项. 相似文献
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Research has shown that the continuation of education by chronically ill adolescents is an important way to avoid social isolation, psychosocial problems and the accumulation of learning difficulties. In this light, virtual learning environments (VLEs), which connect sick adolescents to their schools, play an important role in ensuring that the sick adolescents continue their education, and in contributing to their mental well-being. This study investigated the extent to which a VLE supports the continuation of education and school interactions among sick adolescents and how it is related to their mental well-being. The results show that the VLE had a positive impact on supporting the continuation of education and school interactions among chronically sick adolescents and was considered very helpful to their mental well-being. 相似文献
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鸭传染性浆膜炎又称鸭疫里氏杆菌病,是一种主要侵害幼禽的急性或慢性接触性细菌性传染病。本文根据病鸭的发病情况、临床症状、剖检变化及实验室诊断确诊为鸭传染性浆膜炎。并进行了药敏试验,得到了对本分离菌株敏感的药物,用敏感药物配合中药新菌灵对患病鸭进行了治疗,治愈率达93%. 相似文献
20.
黄艳芬 《合肥教育学院学报》2014,(3):56-58
皖籍现代未名社作家韦素园的文学创作大部分完成于1926年底病后,在内容上主要以病患者的独特视角,呈现由于疾病引发的各种心理体验,包括病患者的生命意识和爱情心理等。韦素园于病隙中的碎笔,既是二十世纪中国疾病文学的构成,也是读者窥视患病作家内心世界的重要参照。 相似文献