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1.
Plastids of nongreen tissues import carbon as a source of biosynthetic pathways and energy, and glucose 6-phosphate is the preferred hexose phosphate taken up by nongreen plastids. A cDNA clone encoding glucose 6-phosphate/phosphate translocator (GPT) was isolated from a cDNA library of immature seeds of rice and named as OsGPT. The cDNA has one uninterrupted open reading frame encoding a 42 kDa polypeptide possessing transit peptide consisting of 70 amino acid residues. The OsGPT gene maps on chromosome 8 of rice and is linked to the quantitative trait locus for 1000-grain weight. The expression of OsGPT is mainly restricted to heterotrophic tissues. These results suggest that glucose 6-phosphate imported via GPT can be used for starch biosynthesis in rice nongreen plastids. 相似文献
2.
INTRODUCTIONNongreenplastidsofheterotrophictissuesarecarbohydrate importingorganellesand ,inthecaseofamyloplastsofstoragetissues,thesiteofstarchsynthesis.Investigationofwholetissuesfromavarietyofstarchsynthesizingcropplantsindicatedthathexoseunitswereimpo… 相似文献
3.
Three genes encoding for fungal cell wall degrading enzymes (CWDEs),ech42, nag70 andglu78 from the biocontrol fungusTrichoderma atroviride were inserted into the binary vector pCAMBIA1305.2 singly and in all possible combinations and transformed to rice plants.
More than 1800 independently regenerated plantlets in seven different populations (for each of the three genes and each of
the four gene combinations) were obtained. Theech42 gene encoding for an endochitinase increased resistance to sheath blight caused byRhizoctonia solani, while the exochitinase-encoding gene,nag70, had lesser effect. The expression level of endochitinase but exochitinase was correlated with disease resistance. Nevertheless,
exochitinase enhanced the effect of endochitinase on disease resistance when the two genes co-expressed in transgenics. Resistance
toMagnaporthe grisea was found in all kinds of regenerated plants including that with singlegluc78. A few lines expressing eitherech42 ornag70 gene were immune to the disease. Transgenic plants are being tested to further evaluate disease resistance at field level.
This is the first report of multiple of expression of genes encoding CWDEs fromTrichoderma atroviride that result in resistance to blast and sheath blight in rice.
Project (No. 3997002) supported by the National Natural Science Foundation of China 相似文献
4.
Tong-bao Liu Guo-qing Chen Hang Min Fu-cheng Lin 《Journal of Zhejiang University. Science. B》2009,10(6):434-444
Fasciclin family proteins have been identified as cell adhesion molecules in various organisms. In this study, a novel Magnaporthe oryzae fasciclin-like protein encoding gene, named MoFLP1, was isolated from a subtractive suppressive cDNA library and functionally analyzed. Sequence analysis showed that the MoFLP1 gene contains an open reading frame (ORF) of 1050 nucleotides encoding 349 amino acids with a calculated molecular weight
of 35.85 kDa and a pI of 7.76. The deduced MoFLP1 protein contains a 17-amino acid secretion signal sequence and an 18-amino
acid sequence with the characteristics of a glycosylphosphotidylinositol (GPI) anchor additional signal at its N- and C-terminuses,
respectively. Potential N-glycosylation sites and domains involving cell adhesion were also identified in MoFLP1. Sequence analysis and subcellular
localization by the expression of MoFLP1-GFP fusion construct in M. oryzae indicated that the MoFLP1 protein is probably localized on the vacuole membrane. Two MoFLP1 null mutants generated by targeted gene disruption exhibited marked reduction of conidiation, conidial adhesion, appressorium
turgor, and pathogenicity. Our results indicate that fasciclin proteins play important roles in fungal development and pathogenicity
in M. oryzae.
Project supported by the National Natural Science Foundation of China (No. 30870101) and the Public Welfare Profession (Agriculture)
Research Project (No. 200803008), China 相似文献
5.
This paper proposes a new approach for multi-objective robust control. The approach extends the standard generalizedl
2 (Gl
2) and generalizedH
2 (GH
2) conditions to a set of new linear matrix inequality (LMI) constraints based on a new stability condition. A technique for
variable parameterization is introduced to the multi-objective control problem to preserve the linearity of the synthesis
variables. Consequently, the multi-channel multi-objective mixed Gl
2/GH
2 control problem can be solved less conservatively using computationally tractable algorithms developed in the paper.
Project supported by the National Natural Science Foundation of China (No. 60374028), and the Scientific Research Foundation
for Returned Overseas Chinese Scholars, Ministry of Education (No. [2004]176) 相似文献
6.
Study of the SISO mixedH
2/l1 problem for discrete time systems showed that there exists a unique optimal solution which can be approximated within any
prescribed missing error bound inl
2 norm with solvable suboptimal solutions and solvable superoptimal solutions.
Project supported by the National Natural Science Foundation of China (No. 60174026) and the Scientific Research Foundation
for Returned Overseas Chinese Scholars of Zhejiang Province (No. J20020546) 相似文献
7.
The endangered medicinal herb,Changium smyrnioides can only be found in deciduous forest gaps within the middle to northern subtropical broad-leaved evergreen forest zone of
China. The considerable plasticity of its shoot and root structure helps it to capture light more effectively in winter and
early spring, and to adapt to the soil moisture conditions in its narrow habitat. Another medicinal plant,Anthriscus sylvestris, is of similar economic importance but commonly distributed widely. In contrast toC. smyrnioides, it has low structural plasticity. It is also specialized to adapt to the moist and sunny environment, where habitat, such
as the banks of creeks and rivers, is abundant.
Project supported by the National Basic Research Program (973) of China (No. G2000046805) and the National Natural Science
Foundation of China (No. 39970058) 相似文献
8.
Breast cancer is one of the leading causes of death in women today. Some of the patients are hereditary, with a large proportion
characterized by mutation in BRCA1 and/or BRCA2 genes. In this review, we provide an overview of these two genes, focusing on their relationship with hereditary breast cancers.
BRCA1/2 associated hereditary breast cancers have unique features that differ from the general breast cancers, including alterations
in cellular molecules, pathological bases, biological behavior, and a different prevention strategy. But the outcome of BRCA1/2 associated hereditary breast cancers still remains controversial; further studies are needed to elucidate the nature of BRCA1/2 associated hereditary breast cancers.
Project supported by the National Natural Science Foundation of China (No. 30772510) and the Joint Program of Ministry of
Health and Zhejiang Provincal Government of China (No. WKJ2006-2-008) 相似文献
9.
Magnaporthe oryzae has been used as a primary model organism for investigating fungus-plant interaction. Many researches focused on molecular
mechanisms of appressorium formation to restrain this fungal pathogen. Autophagy is a very high conserved process in eukaryotic
cells. Recently, autophagy has been considered as a key process in development and differentiation in M. oryzae. In this report, we present and discuss the current state of our knowledge on gene expression in appressorium formation and
the progress in autophagy of rice blast fungi.
Project supported by the National Natural Science Foundation of China (Nos. 30671351 and 30870101) and the Hi-Tech Research
and Development Program (863) of China (No. 2002AA245041) 相似文献
10.
Two-component genes are kinds of genetic elements involved in regulation of antibiotic production inStreptomyces coelicolor. DNA microarray analysis revealed thatecrA1/A2, which mapped at distant sites fromred lucus and encode respectively the kinase and regulator, expressed coordinately with genes of Red specific biosynthetic pathway.ecrA1 andecrA2 gene-disruptive mutants were constructed using homogenotisation by reciprocal double crossover. Fermentation data showed
that the undecylprodigiosin (Red) level of production was lower than that of wild-type strain. However, the change of the
actinorhodin (Act) production level was not significant compared with wild type. Thus, these experiment results confirmed
that the two-component systemecrA1/A2 was positive regulatory element forred gene cluster.
Project (No.20172046) supported by the National Natural Foundation of China 相似文献
11.
AtCRE1 is known to be a cytokinin receptor inArabidopsis. TheAtCRE1 protein contains CHASE domain at the N-terminal part, followed by a transmitter (histidine kinase) domain and two receiver
domains. The N-terminal CHASE domain ofAtCRE1 contains putative recognition sites for cytokinin. Five CHASE domains containing proteins were found in rice,osCRL1a,OsCRL1b,OsCRL2,OsCRL3, andOsCRL4.OsCRL1a,OsCRL1b,OsCRL2 andOsCRL3 contain the four domains existing inCRE1, whereasOsCRL4 only contains the CHASE domain and a putative Ser/Thr protein kinase domain. The authors cloned the encoding geneOsCRL4 and found that it represents a new member of the cytokinin receptor protein in rice.
Project supported by the National Natural Science Foundation of China 相似文献
12.
Study of the morphology, aggregation structure and properties ofBombyx mori silk treated by low temperature oxygen plasma showed that slight flutes appeared on the surface ofBombyx mori silk fiber and that its surface structure changed after plasma treatment. The conformation also changed and crystalline degree
decreased. The stannic filling rate of treated fiber was improved. Because of etching, the weight of the fiber decreased but
the breaking strength changed little after short-time treatment.
Project (No. 50073013) supported by the National Natural Science Foundation of China 相似文献
13.
The long-arm and short-arm genes of fibroin light chain (L-chain) of silkworm,Bombyx Mori L., and the gene of human acidic fibroblast growth factor were cloned respectively and subsequently inserted into a transfer
vector pVL 1392 used as a tool to target the L-chain region of the silkworm genome. Genomic DNA from their offsprings was
extracted and the expected targeting was detected using polymerase chain reaction and DNA sequencing, as well as protein analysis.
The results showed that positive events occurred and that the FGF gene was integrated into the L-chain locus through homologous
recombination.
Project supported by the Scientific Research Foundation for Returned Overseas Chinese Scholars, Education Ministry of China
and the Natural Science Foundation of Zhejiang Province (No. 301306), China 相似文献
14.
1 Introduction Inhomogenetiesintrafficrefertothosefactorsre sultingfromthechangeintrafficflowvolumes ,suchasthechangeinthenumberoflanes ,thedegradationofroads,andthedisturbanceatazebracrossingoratollstation ,etc .Theconsiderationoftheinhomo geneitiesgivesrisetospatiallyvaryingfluxintrafficcontinuummodels ,whichareusuallynon strictlyhy perbolicandhavenotbeenwellunderstoodmathemat ically .Therelatedstudiesinliteraturewerefewandthebehaviorandtheinfluenceoftheseinhomo geneitieswerenotclearenough… 相似文献
15.
ZHANG Xiu-yan RUAN Hui MU Lin HE Guo-qing TANG Xing-jun CHEN Qi-he 《浙江大学学报(A卷英文版)》2006,7(11):1948-1955
INTRODUCTION Enzymes are efficient and specific biocatalysts widely used in food industries, but their application in industrial process often involves special properties not found in natural source enzymes. In order to ob- tain desirable enzymes, in the past ten years, scientists have developed rational (Kurth et al., 1998; Mouratou et al., 1999; DeSantis et al., 1999) and irrational de- sign methods (Babbitt and Gerlt, 1997; O’Brien and Herschlag, 1999) to improve the enzyme propert… 相似文献
16.
In this paper, the Tφ-convex functions were introduced as a generalizations of convex functions. Then the characteristics of the Tφ-convex functions were discussed. Furthermore, some new inequalities for the Tφ-convex functions were derived. 相似文献
17.
The sufficient and necessary conditions of the Bochner-Orlicz sequence spaces equipped with the Luxemburg norm,which have λ property,are given.The result shows,which is not as usual,that the λ property of the Bochner-Orlicz seauence spaces can not be lift from X. 相似文献
18.
Cheng-qi Yan Kai-xian Qian Gang-ping Xue Zhong-chang Wu Yue-lei Chen Qiu-sheng Yan Xue-qing Zhang Ping Wu 《浙江大学学报(A卷英文版)》2004,5(10):1199-1205
Novel bacterial blight (BB) resistance gene(s) for rice was (were) introduced into a cultivated japonica rice variety Oryza sativa (cv. 8411), via somatic hybridization using the wild rice Oryza meyeriana as the donor of the resistance gene(s). Twenty-nine progenies of somatically hybridized plants were obtained. Seven somatically
hybridized plants and their parents were used for AFLP (amplified fragment length polymorphism) analysis using 8 primer pairs.
Results confirmed that these plants were somatic hybrids containing the characteristic bands of both parents. The morphology
of the regenerated rice showed characters of both O. sativa and O. meyeriana. Two somatic hybrids showed highest BB resistance and the other 8 plants showed moderate resistance. The new germplasms with
highest resistance have been used in the rice breeding program for the improvement of bacterial blight resistance.
Project (No. 98001) supported by Ningbo Agriculture Key Scientific Research Foundation, China 相似文献
19.
A genetic linkage map comprised of 131 loci was constructed with an F2 population derived from an inter-subspecific cross betweenBrassica campestris L. ssp.chinensis cv. ‘aijiaohuang’ and ssp.rapifera cv. ‘qisihai’. The genetic map included 93 RAPD loci, 36 AFLP loci and 2 morphological loci organized into 10 main linkage
groups (LGs) and 2 small groups, covering 1810.9cM with average distance between adjacent markers being approximately 13.8cM.
The map is suitable for identification of molecular markers linked to important agronomic traits, QTL analysis, and even for
marker-assisted selection in breeding programs of Chinese cabbage and turnip.
Project (No. 39870509) supported by the National Natural Science Foundation of China 相似文献
20.
β-glucanase was purified from a solid-state culture ofTrichoderma reesei on wheat bran in three steps which comprised ammonium sulfate precipitation, Sephadex G-100 chromatography, and DEAE-Sephadex
A-50 chromatography. The molecular mass was determined to be 35.21 kilodaltons by sodium dodecyl sulfate-12.5% polyacrylamide
gel electrophoresis. The β-glucanase at low pHs was more stable than that at high pHs, and optimum pH was 5.0. The optimum
temperature was 60°C, and β-glucanase was relatively stable at below 40°C for 60 min. TheK
m of the enzyme on β-glucan was 10.86 mg/ml, and theV
max on β-glucan was 14286 μmol of glucose equivalents per mg of the pure enzyme per min. The β-glucanase activity was significantly
inhibited by Fe3+ ions, and was reduced in the presence of Cu2+ ions, Mn2+ ions and Mg2+ ions at 5 mmol/L and 10 mmol/L, respectively. The β-glucanase activity was stimulated by Co2+ ions, Ca2+ ions, Zn2+ ions, and Fe2+ ions at 1 mmol/L and 5 mmol/L, respectively.
Project supported by Foundation for University Key Teacher of the State Ministry of Education, the National Natural Science
Foundation of China (No. 30000118) and Zhejiang Provincial Natural Science Foundation of China (No. 399409). 相似文献