共查询到18条相似文献，搜索用时 187 毫秒
1.
纤维蛋白原(fibrinogen,Fg)是凝血因子I,在体内经凝血酶(thrombin)作用下转变为纤维蛋白,发挥其凝血和止血功能。本研究通过反转录的方法,从杭州人肝脏中成功克隆到人纤维蛋白原α、β、γ基因,并将其定向插入原核表达载体中,人纤维蛋白原α、β、γ基因经成功诱导获得表达,进一步纯化获得人纤维蛋白原α、β、γ蛋白。序列经比对,与Genbank上公布的序列同源性达97%～99%,这项研究为我国汉族人群纤维蛋白原基因核苷酸多态性和单倍型研究发挥一定作用,并且对纤维蛋白封闭剂的开发,提供了重要的产业化实验依据和技术基础。 相似文献
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用盐析－双缩脲法对９７例健康人和７９例冠心病患者进行了血浆纤维蛋白原含量的测定，结果表明：冠心病患者血浆纤维蛋白原含量显著高于健康人（Ｐ＜０．０１），提示纤维蛋白原含量升高是冠心病、中风、血栓形成的一种重要的危险因素．对血浆纤维蛋白原进行检测，将为冠心病的临床诊断及预防提供依据 相似文献
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目的:阐述实验动物在血液制品生产质量控制过程中应用的重要性.方法:按<中华人民共和国药典>2005年版三部要求及相关项目的技术规范进行血液制品质量控制.结果:血浆数量150吨,可同时生产以下品种的产品,人血白蛋白共30批次,总瓶数约40万瓶(折10g);免疫球蛋白类:共30批次,总瓶数约30万瓶(折2.5g);人纤维蛋白原:共30批次,总瓶数约15万瓶(折0.5g);人凝血酶原复合物:共30批次,总瓶数约12万瓶(折300IU);人凝血因子Ⅷ:共30批次,总瓶数约10万瓶(折200IU).需使用家兔870只、豚鼠300只小鼠750只.结论:实验动物应用于血液制品生产质量控制发挥了非常重要的作用. 相似文献
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本文测定了68例原发性高血压患者的血浆纤维蛋白原浓度,并与50例正常血压者作了对照,发现原发性高血压组的血浆纤维蛋白原浓度为700.3±211.4 mg/dl,比对照组高(P相似文献
6.
目的研究适合的蛋白纯化和病毒灭活工艺,制备静脉注射用人SARS特异性免疫球蛋白.方法采用低温乙醇和离子交换层析相结合的工艺进行分离纯化,在分离纯化过程中对血浆进行S/D病毒灭活,对原液进行纳米膜过滤去除病毒.对产品的SARS抗体效价、灭活剂残余含量进行检测,对血浆S/D病毒灭活工艺进行验证.结果产品的各项质量指标符合静脉注射要求,产品中SARS抗体效价经ELISA法检测为183,产品中灭活剂TNBP和Triton-X100的残余含量均＜5.0 ug/ml.病毒灭活方法经验证可以有效灭活指示病毒.结论本蛋白纯化工艺可操作性强、产品质量稳定,为从突发病毒性疾病患者康复期血浆中小规模提取特异性免疫球蛋白提供了一种适合的方法. 相似文献
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目的：研究适合的蛋白纯化和病毒灭活工艺，制备静脉注射用人SARS特异性免疫球蛋白。方法：采用低温乙醇和离子交换层析相结合的工艺进行分离纯化，在分离纯化过程中对血浆进行S／D病毒灭活，对原液进行纳米膜过滤去除病毒。对产品的SARS抗体效价、灭活剂残余含量进行检测，对血浆S／D病毒灭活工艺进行验证。结果：产品的各项质量指标符合静脉注射要求，产品中SARS抗体效价经ELISA法检测为1：83，产品中灭活剂TNBP和Triton-X100的残余含量均〈5．0ug／ml。病毒灭活方法经验证可以有效灭活指示病毒。结论：本蛋白纯化工艺可操作性强、产品质量稳定，为从突发病毒性疾病患者康复期血浆中小规模提取特异性免疫球蛋白提供了一种适合的方法。 相似文献
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《西藏科技》2015,(4)
目的研究探讨高原地区早产儿生后24小时内的凝血功能状态及凝血异常的早产儿输血浆后疗效分析以降低早产儿死亡率。方法选择高原地区收住西藏自治区第一人民医院的40例早产儿及40例足月儿,检测这两组新生儿生后24小时内纤维蛋白原时间(FIB)、凝血酶时间(PT)、部分活化凝血活酶时间(APTT)、凝血酶时间(TT)、血小板(PLT)计数,比较分析这两组患儿生后24小时内的凝血功能状态,对其凝血结果进行回顾性分析,并且对早产儿凝血功能低下的给予输血浆后复查凝血全套,对输血浆后前后进行比较分析。结果此两组(早产儿组与足月儿组)结果相比较,早产儿组的APTT、TT、PT明显增高(P0.05),FIB明显降低(P0.05),D-D明显增高(P0.05),PLT差异及血小板平均体积及血小板比积、血小板体积分布宽度比较后无统计学意义。结论早产儿凝血功能比足月儿低下,高原地区患儿本身存在于缺氧的环境中,如其存在窒息情况,窒息早产儿表现更为明显,凝血功能均有异常,更易发生脐部出血、穿刺处出血不止,严重者发生颅内出血、肺出血等,早期进行凝血功能的常规检测对诊断、指导治疗及预后均有重要意义,临床应重视早产儿凝血功能状态,并对早产儿凝血功能差,FIB1.07g/L的早产儿输血浆,并对输血浆前后进行比较,输血浆后能改善早产儿预后,对降低死亡率及致残率有极其重要的临床意义。 相似文献
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T. Malati B. Yadagiri D. Murali Mohan Krishna V. Shantaram D. Raghunadharao K. Subbarao 《Indian journal of clinical biochemistry : IJCB》2001,16(1):52-59
In the present study, monoclonal gammapathy was identified in a total of 245 patients of plasma cell dyscrasias during period
of 1987 to 2000. The monoclonal band was identified in serum by agar gel electrophoresis in all the cases and in urine in
a few cases. Characterization of paraprotein (monoclonal immunoglobulin class and light chain type) was carried out by employing
immunoelectrophoresis and/or immunofixation electrophoresis using heavy chain specific gamma, alpha, mu, delta and epsilon
and light chain specific kappa (K), lambda (λ) antisera. Serum immunoglobulins Ig G, Ig A, and Ig M were estimated by immunoturbidometry.
Serum urea, creatinine, uric acid, alkaline phosphatase, total proteins, albumin, calcium and phosphorus were estimated by
using routine biochemical methods. Among the 245 cases, 73.1% monoclonal gammapathies were of secretory type and 7.3% were
non-secretory. Monoclonal gammapathies were associated with 80.4% of multiple myeloma, 8.9% of solitary plasmacytoma, 4.1%
of extra-medullary plasmacytoma, 3.3% of lymphoma and 2.9% of plasma cell leukemia. Classification of secretory monoclonal
immunoglobulin revealed monoclonal immunoglobulin Ig G in 74%, Ig A 15% and Ig M in 2.9% cases. 相似文献
12.
Jiaqing Yu Ding Tao Ee Xing Ng Chester L. Drum Ai Qun Liu Chia-Hung Chen 《Biomicrofluidics》2014,8(5)
Thrombin, which has the leading role in the blood coagulation cascade, is an important biomarker in hemostasis and cardiovascular disease (CVD) development. In this study, a measurement system capable of continuously monitoring individual thrombin generation using droplet microfluidic technology is manipulated. The thrombin generation assay based on fluogenic substrate is performed within the droplets and the thrombin generation curve of plasma sample activated by tissue factor is measured in real-time to reflect the sample conditions dynamically. The injection of the inhibitor of thrombin generation is developed to assay the inhibited curve which relates to thrombin self-inhibition in biological systems. This microfluidic system is integrated with the microdialysis probe, which is useful to connect to the living animals for future in vivo real time thrombin measurements for rapid CVD diagnosis. 相似文献
13.
Mohamed Hessien Mohamed Ayad Wafaa M. Ibrahim Batoul Izz ulArab 《Indian journal of clinical biochemistry : IJCB》2015,30(2):210-216
This work was designated to monitor the coagulation abnormalities associated with the gradual progression of liver diseases. The study included fifty patients; forty were diagnosed with liver cirrhosis with different stages categorized according to the Childs-Pugh classification and another ten patients were diagnosed with hepatocellular carcinoma (HCC). Haemostatic variables including fibrinogen (FI), calcium (FIV), transglutaminase (FXIII), prothrombin time (PT) and platelet count were estimated in patients and compared with the baseline levels of healthy subjects (n = 10). The results demonstrated that the fibrinogen level was progressively decreased, whereas PT was progressively prolonged in Child A, Child B and Child C groups. The maximum deterioration was observed in HCC patients. Calcium significantly increased in mild (Child A) and moderate (Child B) but not in Child C cirrhosis and HCC patients. FXIII level did not show any significant changes in cirrhotic patients compared to healthy group. Some of the haemostatic variables we investigated were correlated with serum albumin and bilirubin but not with aminotransferases (ALT and AST). The results indicated that the haemostatic abnormalities in fibrinogen, calcium and PT (but not FXIII) were deteriorated in parallel with the gradual regression of the constitutional function of liver. 相似文献
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Hitender Singh Batra Parduman Singh Babu Lal Somani Ashish Gupta Sangeetha Sampath Vivek Ambade 《Indian journal of clinical biochemistry : IJCB》2007,22(2):106-108
Serum ferroxidase and albumin levels were determined in 98 patients of tubercuiosis, of whom 49 were freshly diagnosed, sputum
positive (group-I) & 49 were completely treated patients (group-II). Forty nine age and sex matched healthy individuals were
taken as controls. Mean±SD of serum ferroxidase and albumin levels in controls, group-I and group-II was found to be 864.35±106.35
IU/L & 3.91±0.234 g/dL, 1603.76±222.65 IU/L & 3.24±0.518 g/dL and 1001.78±201.63 IU/L & 3.82±0.43 g/dL, respectively. Serum
ferroxidase in group I was significantly higher as compared to controls and group-II (p<0.01). The decreased levels of serum
albumin in group I, as compared to control and group-II was statistically significant (p<0.01). Serum ferroxidase: albumin
ratio (Ferroxidase in International Unit per gram of albumin) in group I (50.47±10.36 IU/g) was significantly higher than
controls (22.22±3.3 IU/g), (p<0.001) while in group II it was significantly lower (26.72±7.18 IU/g, p<0.001) than group-I
and close to control values. Serum ferroxidase: albumin ratio (IU/g) can therefore be incorporated as a surrogate marker to
assist in diagnosis and prognosis of pulmonary tuberculosis. 相似文献
15.
Subbiah Pugazhenthi P. Suryanarayana Murthy 《Indian journal of clinical biochemistry : IJCB》1995,10(1):19-22
A new method was developed to purify partially an orally active hypoglycemic fraction from the unripe fruits ofMomordica charantia Linn. The residue after centrifugation of the fruit juice was extracted with ethanol and the concentrated ethanolic extract was further extracted with diethyl ether. In normal rabbits after oral administration, the ether extract at a dose of 0.75 g/kg body wt decreased the fasting blood glucose by 26% (P<0.01). It was compared with charantin, the isolation procedure of which led to loss of hypoglycemic activity many times. Our new method of isolation is simple and the hypoglycemic effect is retained more consistently after purification. 相似文献
16.
Crispin Szydzik Khashayar Khoshmanesh Arnan Mitchell Christian Karnutsch 《Biomicrofluidics》2015,9(6)
Microfluidic based blood plasma extraction is a fundamental necessity that will facilitate many future lab-on-a-chip based point-of-care diagnostic systems. However, current approaches for providing this analyte are hampered by the requirement to provide external pumping or dilution of blood, which result in low effective yield, lower concentration of target constituents, and complicated functionality. This paper presents a capillary-driven, dielectrophoresis-enabled microfluidic system capable of separating and extracting cell-free plasma from small amounts of whole human blood. This process takes place directly on-chip, and without the requirement of dilution, thus eliminating the prerequisite of pre-processed blood samples and external liquid handling systems. The microfluidic chip takes advantage of a capillary pump for driving whole blood through the main channel and a cross flow filtration system for extracting plasma from whole blood. This filter is actively unblocked through negative dielectrophoresis forces, dramatically enhancing the volume of extracted plasma. Experiments using whole human blood yield volumes of around 180 nl of cell-free, undiluted plasma. We believe that implementation of various integrated biosensing techniques into this plasma extraction system could enable multiplexed detection of various biomarkers. 相似文献
17.
Ewa St?pień Krzysztof Gruszczyński Przemys?aw Kapusta Artur Kowalik Iwona Wybrańska 《Biochemia medica : ?asopis Hrvatskoga dru?tva medicinskih biokemi?ara / HDMB》2015,25(2):222-229
Introduction
Centrifugation is an essential step for plasma preparation to remove residual elements in plasma, especially platelets and platelet-derived microparticles (PMPs). Our working hypothesis was that centrifugation as a preanalytical step may influence some coagulation parameters.Materials and methods
Healthy young men were recruited (N = 17). For centrifugation, two protocols were applied: (A) the first centrifugation at 2500 x g for 15 min and (B) at 2500 x g for 20 min at room temperature with a light brake. In protocol (A), the second centrifugation was carried out at 2500 x g for 15 min, whereas in protocol (B), the second centrifugation involved a 10 min spin at 13,000 x g. Thrombin-antithrombin (TAT) and plasmin-antiplasmin (PAP) complexes concentrations were determined by enzyme-linked immunosorbent assays. PMPs were stained with CD41 antibody and annexin V, and analyzed by flow cytometry method. Procoagulant activity was assayed by the Calibrated Automated Thrombogram method as a slope of thrombin formation (CAT velocity).Results
Median TAT and PAP concentrations did not differ between the centrifugation protocols. The high speed centrifugation reduced the median (IQR) PMP count in plasma from 1291 (841-1975) to 573 (391-1010) PMP/µL (P = 0.001), and CAT velocity from 2.01 (1.31-2.88) to 0.97 (0.82-1.73) nM/min (P = 0.049). Spearman’s rank correlation analysis showed correlation between TAT and PMPs in the protocol A plasma which was (rho = 0.52, P < 0.050) and between PMPs and CAT for protocol A (rho = 0.74, P < 0.050) and protocol B (rho = 0.78, P < 0.050).Conclusion
Centrifugation protocols do not influence the markers of plasminogen (PAP) and thrombin (TAT) generation but they do affect the PMP count and procoagulant activity.Key words: cell-derived microparticles, blood coagulation tests, centrifugation, preanalytical phase 相似文献18.
Serum Urea:Albumin Ratio as a Prognostic Marker in Critical Patients With Non-Chronic Kidney Disease
D. B. Gundpatil B. L. Somani T. K. Saha M. Banerjee 《Indian journal of clinical biochemistry : IJCB》2014,29(1):97-100
Routine laboratory investigations play an important role in estimating the risk of mortality in intensive care unit (ICU) patients. The significance of urea:albumin ratio (UAR) in predicting the stay and mortality of ICU patients is not known. It is a retrospective study of patients admitted to ICU (n = 412) with non-chronic kidney disease (non-CKD). Receiver-operating characteristics (ROC) analysis for predicting mortality was carried out to find area under curve (AUC) and threshold levels. Analysis of survival probability was carried out by Kaplan–Meier method and Log-rank test. The AUC to predict mortality were 0.695, 0.767 and 0.791 for serum albumin, urea and UAR, respectively. The threshold levels for albumin, urea and UAR were 2.8 g/dL, 53 mg/dL, and 23.44 mg/g, respectively. The highest odds ratio (OR) of 9.75 to predict mortality at threshold level was observed for UAR, while OR were 7.0 and 3.62 for serum urea and albumin, respectively. The serum urea above and albumin below threshold level were associated with increase in ICU stay of >3 days but the highest OR of 4.73 to predict stay of >3 days was observed for UAR. Kaplan–Meier survival analysis shows significant (p < 0.001) difference at the threshold value of UAR. Serum urea and albumin are found to be an independent predictor for the mortality and stay; however an increased UAR value is the best parameter in predicting mortality and stay in ICU patients with non-CKD illness. 相似文献