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为有效控制家畜布鲁氏菌病发生及流行,百色市在1980-2013年期间采取"监测、扑杀病畜、免疫、检疫、消毒、推广人工授精技术"为主的综合防治技术措施进行布鲁氏菌病防治。采用虎红平板凝集、试管凝集试验和细菌分离相结合的检测方法,共对45492份猪血清、7064份牛血清、6604份羊血清以及852份家畜(含血清检测阳性家畜)胎衣、死胎、流产物等组织样本进行布鲁氏菌病抗体和病原的检测和分离。结果显示,1980-2006年监测的38842份猪血清样品中,布鲁氏菌病抗体阳性数526份,阳性率1.35%,其中以1986-1988年年平均阳性率最高,达4.03%;而2007-2013年监测的6650份猪血清,全部为阴性。在2003-2013年检测的7064份牛血清样品和6604份羊血清样品,未发现抗体阳性。对1990-2012年852份家畜(含血清检测阳性家畜)胎衣、死胎、流产物等组织样本进行布鲁氏菌病原分离和鉴定,全部为阴性。依照《布鲁氏菌病防治技术规范》规定,百色市家畜布鲁氏菌病防治工作在2007年后达到稳定控制标准要求,防治效果显著。 相似文献
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达古拉 《内蒙古科技与经济》2007,(19):95-96
文章认为,布鲁氏菌病是重要的人畜共患传染病.近年来在内蒙古部分地区的奶牛中不断出现流产、早产、死胎、胎盘滞留的疑似布鲁氏菌病患牛,不仅给奶牛养殖户带来了很大的经济损失,而且还严重威胁着人民群众的身体健康.本试验针对目前内蒙古某些地区疑似奶牛布鲁氏菌病流行的现状,采集患牛流产胎儿的肝、肺、脾等病料及布鲁氏菌血清学检测阳性牛的乳,进行了布鲁氏菌的分离与鉴定. 相似文献
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达古拉 《内蒙古科技与经济》2007,(10S):95-96
文章认为,布鲁氏菌病是重要的人畜共患传染病。近年来在内蒙古部分地区的奶牛中不断出现流产、早产、死胎、胎盘滞留的疑似布鲁氏菌病患牛,不仅给奶牛养殖户带来了很大的经济损失,而且还严重威胁着人民群众的身体健康。本试验针对目前内蒙古某些地区疑似奶牛布鲁氏菌病流行的现状,采集患牛流产胎儿的肝、肺、脾等病料及布鲁氏菌血清学检测阳性牛的乳,进行了布鲁氏菌的分离与鉴定。 相似文献
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对田东县存栏母猪10头以上的规模猪场49个进行仔猪腹泻病调查,每个季度调查一次,共调查196个场次存栏仔猪2934头,结果有28个场次发生该病,按场次统计发病率为14.3%;发病猪611头,发病率为20.8%,病死210头,死亡率为7.16%。采集15个发病猪场,每个猪场各采集1份病死猪样品进行检测,每份检测6种病原,结果有11份能检测到病原阳性,阳性率为73.3%,其中检出猪瘟病毒4份,检出率为26.7%;蓝耳病病毒1份,检出率为6.7%;圆环病毒4份,检出率为26.7%;流行性腹泻病毒2份,检出率为13.3%;轮状病毒2份,检出率为13.3%,有1份样品同时检出猪瘟和蓝耳病病毒,所有病料均未能检出传染性胃肠炎病毒。由此可见,田东县仔猪腹泻病由多种病原共同引起。 相似文献
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我们用布氏杆菌病虎红平板凝集试验抗原,对吉林省延边州部分地区的279头牛进行了布氏杆菌病检测,结果平均阳性率28.67%。为布氏杆菌病的防控提供依据。 相似文献
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通过对山南地区人民医院外科门诊206例女性进行淋病耐瑟茵、沙眼衣原体、滴虫和霉菌检查,结果显示为,检出NG阳性标本19例,阳性率为9．22％;检出CT阳性标本37例,阳性率为17．96％;检出NG／CT同时阳性标本8例,阳性率为3．88％;检出阴道滴虫阳性标本16例,阳性率为7．77％;检出阴道霉菌阳性标本28例,阳性率为13．59％;阴道滴虫和霉菌同时阳性标本2例,阳性率为0．97％。因此,尽可能为性病女性进行性传播性疾病病原体的实验室检查,及早发现合并感染者,给予更有效的临床治疗。 相似文献
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对４３例颖似钩体病神经系统后发症患者血清及６９份正常人血清用４种血清学诊断方法作检测比较，发现ＭＡＴ、ＩＭ－ＥＬＩＳＡ，ＩＣＡＴ和ＥＩＤＴ的阳性率分别为９５．３％、９５．３％、９３．０％和９５．３％，对该病的诊断除了具备流行病学指针和临床病和体征外，选用１或２种血清学诊断方法，即可对钩体病神经系统后发症作出确定诊断，便于及早提供治疗措施。 相似文献
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本文对伤寒LPS-PHA诊断试剂盒进行现场应用评价.来自各医院和不同地区977份不同类型的血清标本作LPS-PHA检测的同时与Widal试验作比较,结果伤寒沙门氏菌血培养阳性46份血清标本前者检出率(86.96％)显著高于后者(67.39％),两者在统计学上有显著差异(X~2=4.999,P<0.025),95份Widal试验阳性血清LPS-PHA阳性率为97.89％,漏检2份系乙型副伤寒抗体阳性者.607份对照血清标本LPS-PHA的假阳性率1.48％,低于Widal试验(4.84％).伤寒LPS-PHA的敏感度和特异度(94.33％和98.52％)高于Widal试验(89. 36％和95.72％).其他5项评价指标(阳性和阴性预示值,粗一致性,调整一致性和约登指数)亦都较Widal试验好.因此,LPS-PHA诊断试剂盒能满足目前临床上早期快速诊断伤寒的要求.但不适用于追溯诊断和血清流行病学调查. 相似文献
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J. Pramanik A. N. Lodam C. M. Badole M. V. R. Reddy K. R. Patond B. C. Harinath 《Indian journal of clinical biochemistry : IJCB》2000,15(1):22-28
Trichloroacetic acid (TCA) solubilized and DEAE fractionatedMycobacterium tuberculosis H37Ra excretory-secretory (ES) antigen viz., Mtb EST DE1 and affinity purified goat antibodies to the TCA solubilized ES antigen
(Mtb EST) were explored in detecting tubercular antibody and antigen respectively in sera of bone and joint tuberculosis by
indirect and sandwich ELISA. Out of total 36 bone & joint tuberculosis cases, tubercular antibody was detected by indirect
ELISA in 30 patients (sensitivity 83%), while circulating tubercular antigen was detected by sandwich ELISA in 27 patients
(sensitivity 75%). Out of 34 non tubercular disease control cases, 10 patients showed positive reaction for antibody while
only 4 patients showed positive reaction for antigen. In another group of 34 healthy subjects who were screened, 4 individuals
showed positive reaction for tubercular antibody and 2 cases for antigen. This study shows that antigen detection assay using
affinity purified anti Mtb EST antigen antibody is superior with overall specificity of 91% as compared to antibody detection
assay with 75% specificity in bone & joint tuberculosis. 相似文献
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E. Raji Nair Satish Kumar M. V. R. Reddy B. C. Harinath 《Indian journal of clinical biochemistry : IJCB》1998,13(2):98-105
A mycobacterial excretory-secretory protein fraction ESAS-7 purified by 50% ammonium sulphate precipitation followed by SDS-PAGE
fractionation was evaluated by penicillinase enzyme linked immuno-sorbent assay (ELISA) for its sensitivity and specificity
in the diagnosis of pulmonary tuberculosis. At a “cut off” serum dilution of 600, 38 (90%) of 42 sera from bacteriologically
confirmed tuberculosis cases, 15 (100%) of 15 sera from bacteriogically negative but anti tubercular therapy (ATT) responded
cases, 3 (7%) of 43 sera from normal healthy subjects and 4 (8%) of 48 sera from non tuberculous disease control cases gave
positive reaction for tubercular antibody to ESAS-7 antigen fraction containing predominantly 33-kDa protein with a sensitivity
of 90% in bacteriologically confirmed cases and specificity of 92%. Further, this diagnostic assay using the ESAS-7 antigen
is more sensitive requiring as little as one nanogram antigen per test compared to use of 100 nanogram EST-6 antigen reported
earlier. Thus use of ESAS-7 antigen for antibody detection has good diagnostic potential with improved specificity in pulmonary
tuberculosis. 相似文献
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应用聚合酶链反应（ＰＣＲ）检测了１５３例各型乙型肝炎患者的血清ＨＢＶＤＮＡ，并与ＥＬＩＳＡ法检测的血清ＨＢｅＡｇ和抗ＨＢｅ结果比较．结果ＨＢｅＡｇ阳性８９例，ＰＣＲ法ＨＢＶＤＮＡ检出率为９２．１３％；抗ＨＢｅ阳性２７例，ＰＣＲ法ＨＢＶＤＮＡ检出率为５１．８５％；ｅ系统用性３７例，ＰＣＲ法ＨＢＶＤＮＡ检出率为４０．５４％．提示抗ＨＢｅ血清学转换不能作为乙型肝炎病毒复制与非复制、病变活动与静止的唯一指标，而应该用或同时用ＨＢＶＤＮＡ作为指标才可靠． 相似文献
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Effect of magnesium ions on the binding of bilirubin to erythrocytes of different mammalian species, namely, human, buffalo,
goat and sheep was studied. Increase in the concentration of magnesium ions led to a gradual increase in the erythrocyte-bound
bilirubin in both human and buffalo erythrocytes whereas in sheep and goat erythrocytes, the pronounced increase was found
beyond 2.0 and 2.7 mM MgCl2 concentrations respectively. Percentage increase in erythrocyte-bound bilirubin was found highest in human erythrocytes followed
by buffalo and sheep erythrocytes and minimum in goat erythrocytes. These differences in the binding of bilirubin to different
mammalian erythrocytes can be attributed to the differential shielding effect of metal ions which involves the masking of
negatively charged phosphate of phospholipids found on the erythrocyte surface. 相似文献
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R. Alli B. Bhunia G. P. Chhotray M. V. R. Reddy B. C. Harinath 《Indian journal of clinical biochemistry : IJCB》2003,18(1):61-64
Sera samples of 7 microscopic haematuria cases collected before and after treatment with Diethylcarbamazine citrate, (DEC),
9 microfilaraemic cases and 19 endemic normal individuals were analysed for filarial antigen and IgG antibody levels. Filarial
antigen was detected in 5 of the 7 microscopic haematuria cases, of which 3 turned negative for antigen after treatment with
DEC. While none of the 7 haematuria cases were positive for filarial IgG antibodies, before the DEC treatment, all of them
turned positive after DEC treatment. The sensitivity and specificity values(to detect mf +ve cases) were 89% and 90% respectively
for the detection of filarial antigen and 78% and 95% respectively for the detection of filarial IgG antibodies. 相似文献
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Gian Paolo Caviglia Luca Cabianca Sharmila Fagoonee Fabrizio M. Gili 《Biochemia medica : ?asopis Hrvatskoga dru?tva medicinskih biokemi?ara / HDMB》2016,26(1):114-120
Introduction
Screening programs for colorectal cancer (CRC) are mainly based on a first-line fecal immunochemical test for hemoglobin (FIT). Fecal M2-type pyruvate kinase (M2-PK) has been evaluated in clinical settings showing promising results for early CRC detection. However, the impact of fecal M2-PK assessment on the performance of first-round CRC screening programs is not known. We investigated whether fecal M2-PK alone or in combination with FIT may improve CRC screening efficacy in the general population.Materials and methods
A total of 1027 asymptomatic subjects (median age 66 [59-74] years; females 504 [49.1%]), identified through the general practitioners’ rosters, were invited for the collection of 2 fecal samples for FIT and M2-PK evaluation. Participants with at least positive one fecal test were referred for colonoscopy. Quality indicators for screening performance were calculated and analyzed using Fisher’s exact test.Results
Overall, 572 subjects underwent both FIT and M2-PK assessment (participation rate 55.7%): 93 participants showed positive results for at least one test (positivity rate 16.3%). Only 10 patients were positive for both tests. Attendance rate to colonoscopy was 86.0% and a total of 65 adenomas and 7 cancers were detected. Combined use of FIT and fecal M2-PK permitted the identification of 18 more neoplasm (25%) without improving colonoscopy workload, as deduced by the comparable number needed to scope (P = 0.402).Conclusion
The addition of M2-PK testing to FIT offers the potential to detect additional neoplasms that either do not bleed or only bleed intermittently without reducing participation rate and without increasing endoscopy workload.Key words: biomarker, colorectal cancer, occult blood, M2-type pyruvate kinase, screening 相似文献18.
Rani James K. Thriveni Girija Ramaswamy Lakshmi Krishnamoorthy Geetashree Mukherjee P. P. Vijayalaxmi Deshmane P. P. Bapsy 《Indian journal of clinical biochemistry : IJCB》2008,23(4):345-351
HER-2 is overexpressed in approximately 20–30% of invasive Breast Cancer. ECD of the HER-2 protein is frequently cleaved and
released into the circulation, where it can be detected by ELISA in up to 45% of patients with metastatic breast cancer. The
objective of our study was to compare the current methods for the detection of HER-2 protein. Tissue HER-2 levels were studied
in 100 breast cancer patients by IHC and compared with serum HER-2 levels by ELISA. IHC frequency was 29%. Serum HER-2 ECD
was positive in 42% of patients. A statistically significant correlation was observed. HER-2 detected by IHC correlates significantly
with serum HER-2 levels detected by ELISA. Thus, ELISA is a reliable and economical tool to assess the HER-2 status in tumors,
when breast tissue sample is not available. 相似文献