共查询到17条相似文献，搜索用时 125 毫秒
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本研究以金线莲茎尖作为外植体,研究了不同激素浓度对金线莲茎尖诱导、增殖及生根率的影响。结果表明：对于金线莲比较适合的诱导培养基是MS＋6-BA4-．0mg／L．＋ZT0．2mg／L＋NAA0．5mg／L;增殖较佳的组合是1／2Ms＋6-BA0-4mg·L-1＋NAA0·2mg·L-1。当NAA的浓度达到2·0mg·L-1时生根率最高,可以达到100％。 相似文献
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硼酸和钙离子对红叶李花粉萌发影响 总被引：1，自引：0，他引：1
通过11种不同的培养基,采用液体培养法研究了硼酸和钙离子对红叶李花粉萌发的影响.结果表明一定浓度的硼酸和钙对花粉的萌发有较大的促进作用,但超过一定浓度时起抑制作用,红叶李花粉萌发的最适硼酸和钙离子浓度分别为100mg/L和500mg/L,在最适培养基上(10%蔗糖 100mg/L硼酸 500mg/L钙离子),红叶李花粉的萌发率为72.56%. 相似文献
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硼酸和钙离子对红叶李花粉萌发影响 总被引：1，自引：0，他引：1
通过11种不同的培养基,采用液体培养法研究了硼酸和钙离子对红叶李花粉萌发的影响。结果表明一定浓度的硼酸和钙对花粉的萌发有较大的促进作用,但超过一定浓度时起抑制作用,红叶李花粉萌发的最适硼酸和钙离子浓度分别为100mg/L和500mg/L,在最适培养基上(10%蔗糖 100mg/L硼酸 500mg/L钙离子),红叶李花粉的萌发率为72.56%。 相似文献
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美国薄荷的组织培养与植株再生 总被引：1，自引：0，他引：1
论述了一种美国薄荷的组织培养与植株再生的试验方法,本试验采用植物组织培养的方法,对美国薄荷的茎段、叶片等外质体进行体外培养,利用多种不同培养基成功诱导出愈伤组织、不定芽和不定根,从而完成了植株再生的目的,得出适合诱导愈伤组织及不定芽的激素浓度配比为：MS＋6-BA1．0mg／Lq-NAA0．2mg／L,诱导根的最佳激素浓度配比为：MS＋NAA0．1mg／L。 相似文献
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本实验以索邦百合为材料,研究了不同培养基对索邦百合外植体的诱导,增值和生根的影响。结果表明：不同的外植体诱导时间不同,最佳外植体是带芽的茎段。MS＋BA0．5mg／L＋NAA0．5mg／L继代繁殖效果最佳,不同生根培养基对索邦继代苗生根影响差异显著,其中以1／2MS不附加任何激素为最佳,生根率为99％,平均每株苗生粗根6．9条。 相似文献
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本文主要研究不同2,4－D浓度和培养条件对愈伤组织诱导的影响,结果表明：诱导愈伤组织最好的配比为 MS培养＋6－BA：1．0mg/L＋NAA：0．1mg/L＋2,4－D：1．0mg/L. 相似文献
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水培条件下绿萝对水体中铅、铬的净化作用 总被引：1，自引：0，他引：1
采用静态水培试验的方法,研究绿萝对不同浓度铅、铬污染水体的净化效果。结果表明绿萝对Pb污染浓度小于1 mg/L的水体有明显的净化效果,Pb的去除率在41%以上,当Pb污染浓度大于5 mg/L的时,绿萝对Pb的去除率趋向于零;绿萝对Cr污染浓度小于1 mg/L的水体有明显的净化效果,Cr的去除率在17%以上,当Cr浓度大于5 mg/L时,绿萝对Cr的去除趋向于0。研究表明,绿萝在净化低浓度Pb,Cr污染的水体方面有较好的应用前景,并且对Pb的去除作用更加明显。 相似文献
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用单扫示波极谱法，青藤碱在0．1mol／LNa_2B_4O_7中有两个还原峰，峰P_1和P_2的电位分别为-1．45V和-1．67V（vs．SCE），峰P_1的峰电流与浓度在0．06～1．8mg／L和20～34．6mg／L范围内有线性关系，检测限为0.02mg／L，用以测定中药膏风藤克总生物碱的含量，结果令人满意．实验证明，青藤碱的电极反应过程为不可逆的四电子过程，并讨论了青藤碱对超氧阴离子自由基（O_2~-）的清除作用。 相似文献
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BackgroundIn order to produce an effective callus in Echinacea purpurea L.; determination of the explant type and growth regulators that best respond to callus induction and the optimization of the culture conditions to increase the amount of caffeic acid derivatives (CADs) in the obtained callus. CADs contents of callus cultures of E. purpurea were evaluated by establishing an effective callus induction system in vitro.ResultsVarious medium containing different growth regulators were tested using leaf, petiole, cotyledon and root as the explants. The best callus development was achieved in MS medium with 1.0 mg l−1 2,4-D + 2.0 mg l−1 BAP in leaf, 1.0 mg l−1 NAA + 0.5 mg l−1 TDZ in petiole, 2.0 mg l−1 NAA + 1.0 mg l−1 TDZ in cotyledon and 0.5 mg l−1 NAA + 0.5 mg l−1 BAP in roots. Upon optimisation of callus growth, each type of explant was cultured for 4, 6, 8 and 10 weeks in medium for the analyses of caftaric acid, chlorogenic acid, caffeic acid and chicoric acid contents. The highest amounts of caftaric acid (4.11 mg/g) and chicoric acid (57.89 mg/g) were found from petiole explants and chlorogenic acid (8.83 mg/g) from root explants at the end of the 10-week culture time.ConclusionsAs a result of the present study, the production of caffeic acid derivatives was performed by providing the optimization of E. purpurea L. callus cultures. Effective and repeatable protocols established in this study may offer help for further studies investigating the production of caffeic acid derivatives in vitro.How to cite: Tanur Erkoyuncu M, Yorgancilar M. Optimization of callus cultures at Echinacea purpurea L. for the amount of caffeic acid derivatives. Electron J Biotechnol 2021;51. https://doi.org/10.1016/j.ejbt.2021.02.003. 相似文献
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根箱法解析大豆苗期根际养分分布及吸收特征 总被引：2，自引：0，他引：2
植物影响根际微生态系统特征。应用根箱法对2个大豆基因型的苗期养分（N、P、K、Ca、Mg、Fe、Mn、Zn和Cu）吸收及其在根际分布特征进行了研究。结果表明，高产基因型北丰11 对N、P、K、Ca、Mg、Mn、Zn和Cu的吸收量均显著高于低产基因型海9731，其单位根长、根表面积吸收N、P、Ca和Mg的量亦较高，其根系吸收活力较强。土壤有效养分在根际的分布趋势不同，有效N和Mn在0–4 mm范围内的含量高于非根际区（>10 mm），而P、K、Mg和Cu在根面区表现一定程度的亏缺。2个基因型间根际养分差含量异较大，除K和Fe外，其它营养元素在北丰11根面和近根面区的含量均不同程度地高于海9731。北丰11根际0–6 mm的范围内pH较低。 相似文献
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M. I. Akpanabiatu I. B. Umoh E. O. Udosen A. E. Udoh E. E. Edet 《Indian journal of clinical biochemistry : IJCB》2005,20(2):29-34
Aqueous extract of the leaf and root ofNauclea latifolia Sm. (Rubiaceae) is used in Nigerian folk medicine for the treatment of hypertension. This work is carried out to investigate
the effect ofNauclea latifolia leaf extract on lipid profile and cardiovascular activity of rats. Normal and 10% coconut oil fed rats were treated with
the water-soluble fraction of the ethanol extract ofNauclea latifolia leaf for 2 weeks. Forty-eight mature male albino rats of the Wistar strain were divided into two experiments of four groups,
each group having 6 animals. Experiment I animals were treated with the water-soluble fraction of the ethanol extract whilst
experiment II animals were fed 10% coconut oil meal before treatment with the water-soluble fraction of the ethanol extract.
A single oral dose ofNauclea latifolia was 170, 340 and 510 mg/kg body wt/day of the extracts respectively for 2 wks. There was no significant change in the lipid
profile of the experimental animals as compared with the controls. There was about 40% relaxation on contracted thoracic aorta
that was pre-contracted with 2 μM phenylephrine. The viability of the tissue was tested against 10 μM of acetylcholine. There
was no significant (P>0.05) change in Na+ concentration in the serum. However, the K+ concentration in the serum of the experimental animals showed a significant increase. The study shows that ethanol extract
ofNauclea latifolia has vasodilator action on the aorta and that lipid profiles of experimental rats were not affected. Furthermore, the increase
in the K+ may be contributing to the vasodilator effect ofNauclea latifolia. 相似文献
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在表面活性剂存在下及硫酸介质中,70℃恒温水浴中,铜（Ⅱ）催化溴酸钾氧化亚甲基蓝褪色。据此建立了测定痕量铜的方法。铜（Ⅱ）浓度的线性范围为0-0．1mg．L^-1,最大吸收波长为660nm,摩尔吸光系数为2．2×10^4L．g^-1．cm^-1,检出限为3．82×10^-6g·L^-1。方法简单快速、选择性好,用于测定水中铜含量时,测定结果与AAS法的结果相近,所得结果的RSD值均小于3．2％,回收率在99—104％。 相似文献
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BackgroundCecropin P1, acting as an antimicrobial, has a broad-spectrum antibacterial activity with some antiviral and antifungal properties. It is a promising natural alternative to antibiotics which is originally isolated from the pig intestinal parasitic nematode Ascaris suum. Many studies have shown that Cecropin P1 is helpful for the prevention or treatment of clinical diseases. Therefore, it is very necessary to establish a safe, nontoxic, and efficient expression method of Cecropin P1.ResultsThe results indicated that the recombinant protein was about 5.5 kDa showed by Tricine–SDS–PAGE and Western blot. And Cecropin P1 was efficiently secreted and expressed after 12 h of induction, with an increasing yield over the course of the induction. Its maximum concentration was 7.83 mg/L after concentration and purification. In addition, in vitro experiments demonstrated that Cecropin P1 not only exerted a strong inhibitory effect on Escherichia coli, Salmonella sp., Shigella sp., and Pasteurella sp., but also displayed an antiviral activity against PRRSV NADC30-Like strain.ConclusionsCollectively, the strategy of expressing Cecropin P1 in Saccharomyces cerevisiae is harmless, efficient, and safe for cells. In addition, the expressed Cecropin P1 has antiviral and antibacterial properties concurrently.How to cite: Jiang R, Zhang P, Wu X, et al., Expression of antimicrobial peptide Cecropin P1 in Saccharomyces cerevisiae and its antibacterial and antiviral activity in vitro. Electron J Biotechnol 2021;50. https://doi.org/10.1016/j.ejbt.2020.12.006 相似文献