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1.
G. Sharmila Banu G. Kumar A. G. Murugesan 《Indian journal of clinical biochemistry : IJCB》2009,24(4):414-418
The aim of this study was to investigate the ethanolic leaf extract of Trianthema portulacastrum L. (Family: Aizoaceae) on
aflatoxin induced hepatic damage in rats. Aflatoxin intoxication in rats significantly (p < 0.001) elevated the levels of
serum glutamate pyruvate transaminase (SGPT), serum glutamate oxaloacetate transaminase (SGOT), alkaline phosphatase (ALP),
and total bilirubin, which indicated acute hepatocellular damage and biliary obstruction. Ethanolic leaf extract of T. portulacastrum
showed dose dependent decrease in the levels of SGPT, SGOT, ALP and total bilirubin. Minimum effective dose of extract was
found to be 100 mg/kg body weight. Results obtained from histopathological studies also supported hepatoprotective activity
against aflatoxin-induced hepatotoxicity. Thus the study demonstrates that T. portulacastrum possess antihepatotoxic effect
against aflatoxin. 相似文献
2.
Present study aimed to evaluate the protective role of the aqueous extract of Phyllanthus niruri (P. niruri) against nimesulide-induced
hepatic disoder in mice by determining levels of glutamate oxaloacetate transaminase (GOT), glutamate pyruvate transaminase
(GPT) and alkaline phosphatase (ALP) in serum and also by measuring the hepatic content of the antioxidant enzymes, superoxide
dismitase (SOD) and catalase (CAT); the free radical scavenger, reduced glutathione (GSH) and thiobarbituric acid reacting
substances (TBARS). Aqueous extract of P. niruri was administered either orally or intraperitoneally in different doses and
times as needed for the experiments. Intraperitoneal of the extract (100 mg/kg body weight for seven days) reduced nimesulide
(750 mg/kg body weight for 3 days) induced increased levels of GOT (37.0±1.8 units/ml in control group vs. 91.8±2.0 units/ml
in nimesulide treated group vs. 35.0±1.0 units/ml in extract treated group), GPT (30.0±2.1 units/ml in control group vs. 88.4±2.9
units/ml in nimesulide treated group vs. 34.1±1.8 units/ml in extract treated group), and ALP (7.86±0.47 KA units/ml in control
group vs. 23.80±0.60 KA units/ml in nimesulide treated group vs. 7.30±0.40 KA units/ml, in extract treated group) to almost
nomal. In addition, P. niruri restored the nimesulide induced alterations of hepatic SOD (550±20 units/mg total protein in
control group vs. 310±13 units/mg total protein in nimesulide treated group vs. 515±10 units/mg total protein in extract treated
group), CAT (99.5±2 units/mg total protein in control group vs. 25.0±1.5 units/mg total protein in nimesulide treated group
vs. 81.0±0.8 units/mg total protein in extract treated group), GSH (90±3 nmoles/mg total protein in control group vs. 17±4.2
nmoles/mg total protein in nimesulide treated group vs. 81±1 nmoles/mg total protein in extract treated group) and TBARS (measured
as MDA, 36.6±3.0 nmoles/g liver tissue in control group vs. 96.3±5.2 nmoles/g liver tissue in nimesulide treated group vs.
41.2±1.7 nmoles/g liver tissue in extract treated group) contents. Dose-dependent studies showed that the herb could protect
liver even if the nimesulide-induced injury is severe. Intraperitoneal administration of the extract showed better protective
effect than oral administration. Combining all, the data suggest that P. niruri possesses hepatoprotective activity against
nimesulide-induced liver toxicity and probably acts via an antioxidant defense mechanism. To the best of our knowledge, this
is the first report of the hepatoprotective action of P. niruri against nimesulide induced liver damage. 相似文献
3.
Menon BR Rathi MA Thirumoorthi L Gopalakrishnan VK 《Indian journal of clinical biochemistry : IJCB》2010,25(4):401-404
The study was designed to evaluate the hepatoprotective activity of ethanolic extract of Bacopa monnieri in acute experimental liver injury induced by Nitrobenzene in rats. The extract at the dose of 200 mg/kg body weight was
administered orally once every day for 10 days. The increased serum marker enzymes, Aspartate transaminase, Alanine transaminase
and alkaline phosphatase were restored towards normalization significantly by the extract. Significant increase in SOD, CAT
and GPx was observed in extract treated liver injured experimental rats. Histopathological examination of the liver tissues
supported the hepatoprotection. It is concluded that the ethanolic extract of Bacopa monieri plant possess good hepatoprotective activity. 相似文献
4.
Caffeic acid is a well-known phenolic compound widely present in plant kingdom. The aim of this study was to investigate the
possible protective effect of caffeic acid (CA) against oxytetracycline (OXT) induced hepatotoxicity in male Albino Wistar
rats. A total of 30 rats weighing 150–170 g were randomly divided into five groups of six rats in each group. Oral administration
of OXT (200 mg/kg body weight/day) for 15 days produced hepatic damage as manifested by a significant increase in serum hepatic
markers namely aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase
(LDH), bilirubin and increased plasma and hepatic lipid peroxidation indices (TBARS and hydroperoxide). The present finding
shows that the levels of enzymatic antioxidants namely superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase
(GPx) were significantly decreased in OXT intoxicated rats. Upon oral administration of caffeic acid (40 mg/kg body weight/day)
there were decreased hepatic marker activities, bilirubin and lipid peroxidation and increased enzymatic antioxidants in OXT + Caffeic
acid group compared to Normal + OXT group(P < 0.05). Our study suggests that caffeic acid has antioxidant property and hepatoprotective ability against OXT induced toxicity. 相似文献
5.
Biochemical activity of selenium and glutathione on country made liquor (CML) induced hepatic damage in rats 总被引:1,自引:0,他引:1
G. Kumar G. Sharmila Banu M. Rajasekara Pandian 《Indian journal of clinical biochemistry : IJCB》2007,22(1):105-108
The serum and hepatic enzymes of rats were studied after exposed to country made liquor (CML) along with two chelating agents
(glutathione and Selenium). There was a significant increase in several serum enzyme levels (viz., aspartate transaminase,
alanine transaminase, alkaline phosphatase, sorbitol dehydrogenase, glutamate dehydrogenase, bilirubin) and decrease in various
hepatic enzymes (Succinic dehydrogenase, Glucose 6-phosphatase, 5'Nucleotiease, Acid phosphatase, Acid ribonuclease, Cytochrome
P-450) due to repeated administration of CML (2ml/100g of body weight). Results of this study revealed that the GSH and Se
could give a significant protective action in serum and hepatic enzymes of CML exposed rats. 相似文献
6.
Present investigation shows that hydroethanolic extract of Moringa oleifera (MOHE) and its isolated saponin (SM) attenuates DMBA induced renal carcinogenesis in mice. Isolation of SM was achieved by TLC and HPLC and characterization was done using IR and 1H NMR. Animals were pre-treated with MOHE (200 and 400 mg/kg body weight; p.o), BHA as a standard (0.5 and 1 %) and SM (50 mg/kg body weight) for 21 days prior to the administration of single dose of DMBA (15 mg/kg body weight). Administration of DMBA significantly (p < 0.001) enhanced level of xenobiotic enzymes. It enhanced renal malondialdehyde, with reduction in renal glutathione content, antioxidant enzymes and glutathione-S-transferase. The status of renal aspartate transaminase, alanine transaminase, alkaline phosphatase and total protein content were also found to be decreased along with increase in total cholesterol in DMBA administered mice. Pretreatment with MOHE and SM significantly reversed the DMBA induced alterations in the tissue and effectively suppressed renal oxidative stress and toxicity. 相似文献
7.
Krishnan Gokuladhas Subramaniyan Jayakumar Balan Rajan Ramasamy Elamaran Chengalvarayan Subramani Pramila Mani Gopikrishnan Sasivarman Tamilarasi Thiruvengadam Devaki 《Indian journal of clinical biochemistry : IJCB》2016,31(2):171-184
Liver cancer is the fifth most common cancer and is still one of the leading causes of death world wide, due to food additives, alcohol, fungal toxins, air, toxic industrial chemicals, and water pollutants. Chemopreventive drugs play a potential role in liver cancer treatment. Obviously in the production of anticancer drugs, the factors like poor solubility, bioavailability, biocompatibility, limited chemical stability, large amount of dose etc., plays a major role. Against this backdrop, the idea of designing the chemopreventive nature of bio flavanoid hesperetin (HP) drug conjugated with pegylated gold nanoparticles to increasing the solubility, improve bioavailability and enhance the targeting capabilities of the drug during diethylnitrosamine (DEN) induced liver cancer in male wistar albino rats. The dose fixation studies and the toxicity of pure HP and HP conjugated gold nanoparticles (Au-mPEG(5000)-S-HP) were analysed. After concluded the dose fixation and toxicity studies the experimental design were segregated in six groups for the anticancer analysis of DEN induced HCC for 16 weeks. After the experimental period the body weight, relative liver weight, number of nodules and size of nodules, the levels of tumor markers like CEA, AFP and the level of lipid peroxidation, lipid hydroperoxides and the activities of antioxidant enzymes were assessed. The administration of DEN to rats resulted in increased relative liver weight and serum marker enzymes aspartate transaminase, alanine transaminase, alkaline phosphatase, lactate dehydrogenase, and gamma glutamyl transpeptidase. The levels of lipid peroxides elevated (in both serum and tissue) with subsequent decrease in the final body weight and tissue antioxidants like superoxide dismutase, catalase, reduced glutathione, glutathione peroxidise, and glutathione reductase. HP supplementation (20 mg/kg b.wt) significantly attenuated these alterations, thereby showing potent anticancer effect in liver cancer and the HP loaded gold nanoparticels (Au-mPEG(5000)-S-HP) treated animals shows the better treatment than the pure HP due to the solubility of drug, bioavailability and the target drug delivery of the biodegradable polymer. Histological observations were also carried out, which added supports to the chemopreventive action of the pure HP and HP loaded gold nanoparticles (Au-mPEG(5000)-S-HP) against DEN induction during liver cancer progression. These findings suggest that HP loaded gold nanoparticels (Au-mPEG(5000)-S-HP) shows better efficacy than the pure HP against lipid peroxidation, hepatic cell damage and protects the antioxidant system in DEN induced hepatocellular carcinogenesis. 相似文献
8.
Subcutaneous administration of monosodium glutamate (MSG), to normal adult male mice, for six consecutive days at dose levels
of 4 and 8 mg/g body weight, significantly increased the level of free radical initiating enzyme, xanthine oxidase, whereas
the activity of free radical scavenging enzymes, like catalase and superoxide dismutase was significantly decreased in hepatic
tissue. These observations suggested that ingestion of MSG at dose level of 4 mg/g body weight and above, induced oxidative
stress in the hepatic tissue of adult male mice. 相似文献
9.
The present study was carried out to investigate the protective role of Triphala (a combination in equal proportions by weight
of fruit powder of Terminalia belerica, Terminalia chebula and Emblica officinalis) against 1,2-dimethylhydrazinedihydrochloride (DMH) induced Endoplasmic reticulum stress (ER stress) in mouse liver. An oral
dose of 3 mg/kg body wt in drinking water for 5 weeks significantly (P < 0.001) increased the levels of serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase
(SGPT), serum Alkaline phosphatase (ALP) and total bilirubin thus suggesting damage to mouse liver and biliary dysfunction.
The DMH administration invariably led to increase in the liver microsomal proteins of molecular weight of about 29 (ERp29)
and 53 kDa (ERp53) and decrease in the protein of molecular weight of 36 kDa (ERp36) thereby suggesting the interference of
DMH and its metabolites with normal protein biosynthesis and folding, in the reticular membranes of the liver cells thus developing
ER stress. Histological studies show necrosis, large sized hepatocytes with increased N:C ratio, aberrant mitotic figures
and prominent nucleoli in the liver of DMH treated mice. In animals fed 5% Triphala in diet (w/w) during DMH administration,
there was significant decrease in the above changes in the liver suggesting the suppression of DMH induced ER stress in liver.
Triphala significantly (P < 0.05) decreased lipid peroxidation and also the activity of lactate dehydrogenase (LDH) in mouse liver. It simultaneously
increased the level of reduced glutathione (GSH) and the activity of glutathione-S-transferase (GST) thereby suggesting that
it prevents peroxidative damage and also diverts the active metabolites (electrophiles) of DMH from their interactions with
critical cellular bio-molecules which could be responsible for its protective action against DMH. 相似文献
10.
Manish K. Singh Shailendra Dwivedi Suraj S. Yadav Praveen Sharma Sanjay Khattri 《Indian journal of clinical biochemistry : IJCB》2014,29(1):29-37
Arsenic a metalloid and environmental contaminated has been found to be associated with public health problems in the affected areas. It is naturally occurred in groundwater and its accumulation in plant and animals leads to toxicity in several tissues most notably hepatic organ. Arsenic exposures (3 mg/kg body weight/day for 30 days) in mice exhibited increased arsenic and Zn levels in hepatocytes associated with enhanced oxidative stress in hepatocytes while there were no significantly changes were observed in Cu level. An increase in the lipid peroxidation and decrease in the levels of reduced glutathione and activity of superoxide dismutase, catalase, and glutathione peroxidase were observed in arsenic treated mice as compared to controls. Arsenic exposure in mice also caused a significant change in serum biomarkers in the SGOT, SGPT and creatinine as compared to the controls. There were no significant changes in the serum levels of total protein in these mice. Co-administration of arsenic and fruit extract of amla (500 mg/kg body weight/day for 30 days) caused a significant reduction of arsenic transference associated with significantly decreases hepatic arsenic levels and balanced the antioxidant enzyme and levels of serum hepatic enzymes like SGOT and SGPT. The results of the present study clearly demonstrate the antioxidant property of amla that could be responsible for its protective efficacy in arsenic induced hepatic toxicity. 相似文献
11.
Biman Bhuyan Krishna Baishya Prakash Rajak 《Indian journal of clinical biochemistry : IJCB》2018,33(2):190-195
Alternanthera sessilis commonly known as ‘sessile joy weed’ or ‘dwarf copperleaf’ is found throughout the hotter parts of India up to an altitude of 1200 m2. In Assam, the plant has been traditionally used in the treatment of jaundice along with other ailments. The study focuses primarily on the evaluation of hepatoprotective activity of the plant with special references to its putative protective role in carbon tetrachloride induced liver injury on Wister albino rat. The in vivo hepatoprotective activity of the methanolic extract at the dose of 250 mg/kg body weight was highly effective in controlling SGPT, SGOT, ALP, serum cholesterol and serum bilirubin level as compared to silymarin. The said extract also significantly lowered the lipid profile caused by CCl4. The activity shown by methanolic extract of the whole plant of A. sessilis is of considered importance and thus justified its use in controlling hepatic diseases in traditional treatment system. 相似文献
12.
The present study was undertaken to analyze the antioxidant (both enzymic and nonenzymic) activities of leaves of Ocimum sanctum hydroalcoholic extract against cadmium induced damage in albino rats. Oral administration of cadmium as CdCl2 (6.0 mg/kg body weight) led to significant elevation of lipid peroxidation (LPO) levels and significantly decreased Superoxide
Dismutase (SOD), Catalase (CAT), Glutathione Peroxidase (GPx), Reduced Glutathione (GSH) and Vitamin C (Ascorbate) levels.
Administration of Ocimum sanctum extract (100 mg/kg body weight, po) and (200 mg/kg body weight, po) before and after cadmium intoxication showed a significant
decrease in LPO levels and significant increase in SOD, CAT, GPx, GSH and Ascorbate levels. The results suggest that oral
administration of Ocimum sanctum extract provides significant protection against cadmium induced toxicity in Wistar albino rats. 相似文献
13.
J. O. Adebayo A. O. Akinyinka G. A. Odewole J. I. Okwusidi 《Indian journal of clinical biochemistry : IJCB》2007,22(1):29-32
The effect of caffeine intake on the risk of coronary heart disease was studied. Twenty-one rats used were randomly divided
into three experimental groups, the first group served as the control while the second and third groups were administered
caffeine orally at doses of 10mg/kg body weight and 20mg/kg body weight respectively for fourteen days. Caffeine, at 10mg/kg
body weight, significantly increased (P<0.05) serum LDL- cholesterol concentration and coronary heart disease risk ratio while
it significantly reduced (P<0.05) serum triacylglycerol concentration when compared with controls. At 20mg/kg body weight,
caffeine significantly increased (P<0.05) coronary heart disease risk ratio while it significantly reduced (P<0.05) serum
HDL-cholesterol concentration and serum triacylgycerol concentration when compared with controls. No dose response effect
was observed possibly suggestive of a threshold effect. These results suggest that caffeine predisposes consumers of caffeine
containing beverages to coronary heart disease. 相似文献
14.
4mg and 8mg monosodium glutamate per gram body weight was administered subcutaneously for 6 consecutive days to normal adult
male mice and its effect was seen on 31st day after the last injection on some antioxidant enzymes in heart. A significant dose dependent increase in lipid peroxidation
and xanthine oxidase level was observed, whereas the activity of free radical scavenging enzymes such as superoxide dismutase
and catalase was decreased in both monosodium glutamate treated groups (Group-2 and Group-3). So, the present work suggested
that monosodium glutamate at dose level of 4mg/g body weight and above induced oxidative stress in the cardiac tissue by changing
the activity of free radical initiating enzyme such as xanthine oxidase and scavenging enzymes like superoxide dismutase and
catalase. 相似文献
15.
P. K. Rai D. Jaiswal S. Mehta D. K. Rai B. Sharma Geeta Watal 《Indian journal of clinical biochemistry : IJCB》2010,25(2):175-181
This study deals with the effects of freeze dried rhizome powder of Curcuma longa (C. longa) dissolved in milk on normal as
well as diabetic models. Diabetes of type II and type I was within 3 days of a single administration of doses of 45 and 65
mg kg−1 of streptozotocin respectively. Various parameters such as blood glucose levels, triglycerides, total cholesterol, high density
lipoprotein, very low density lipoprotein, low density lipoprotein, serum glutamic oxaloacetic transaminase, serum glutamic
pyruvate transaminase, alkaline phosphatase, creatinine, hemoglobin, urine protein and urine sugar in addition to body weight
were taken in to consideration and were analyzed after administration of variable doses of rhizome powder. The dose of 200
mg kg−1 was identified as the most effective dose as it increased HDL, Hb and bw (P<0.05) with significant decrease in the levels
of blood glucose, lipid profile and hepatoprotective enzymes (P<0.001). 相似文献
16.
M. Mastan U. V. Prasad P. R. Parthasarathy 《Indian journal of clinical biochemistry : IJCB》2007,22(1):122-127
Bacopa monniera, a medicinal plant distributed throughout India. Cytosine arabinoside (1-β-arabinofuranosylcytosine; Ara-C)
is the most important antimetabolite chemotherapeutic drug used for acute leukemia. In this study we examined the chemoprotective
property of an ethanolic extract of Bacopa monniera on biochemical changes in chick embryo. CA caused biochemical changes
in a concentration and time dependent manner in amniotic fluid, liver and heart tissues. Ethanolic extract of BM given to
chick embryo at doses of 2, 4, 6 mg per egg. There is significant decrease in biochemical levels of glucose, protein, urea,
uric acid, creatinine and inorganic phosphorus. Enzymatic activities of alkaline phosphatase, lactate dehydrogenase, serum
glutamate oxaloacetate transaminase, serum glutamate pyruvate transaminase and malatedehydrogenase were also decreased with
dose dependent manner in amniotic fluid, liver and heart tissues. 相似文献
17.
Ramesh Chander Farhan Rizvi A. K Khanna Ram Pratap 《Indian journal of clinical biochemistry : IJCB》2003,18(2):71-79
Guggulsterone, a mixture of cis (E) and trans (Z) isomers (7∶3 w/w) was synthesized from 16-DPA. The isomers were separated
by column chromatography and evaluated for cardioprotective and antioxidant activities. Myocardial necrosis induced by isoproterenol
in rats caused marked increase in serum creatine phosphokinase and glutamate pyruvate transaminase. Simultaneously in ischemic
heart, phospholipase, xanthine oxidase and lipid peroxides were enhanced following depletion of glycogen, phospholipids and
cholesterol. Treatment with guggulsterone and its both isomers at the dose of 50 mg/kg po., significantly protected cardiac
damage as assessed by the reversal of blood and heart biochemical parameters in ischemic rats. The cardioprotective activity
of guggulsterone and of both the isomers were compared with that of gemfibrozil at the same doses. Guggulsterone and both
the isomers at tested concentrations (5–20mM) inhibited oxidative degradation of lipids in human low-density lipoprotein and
rat liver microsomes induced by metal ionsin vitro. The drug counteracted against the generation of superoxide anions (O2) and hydroxyl radicals (OH−) in non-enzymic test systems. It is suggested that cardioprotective and antioxidant activities of synthetic guggulsterone
and guggulsterone obtained from gum resinCommiphora mukul that contains isomers E & Z in the ratio of 46∶54w/w are the same. 相似文献
18.
Curcumin is a natural anti-inflammatory and antioxidant with several potential health benefits. Although it has been examined in several metals toxicity studies, but its role in the protection against copper toxicity has not been investigated. In this study; the detoxification and antioxidant effect of curcumin were examined to determine its prophylactic/therapeutic role experimentally in rats. Forty albino rats were divided into five groups; control, CuSO4 (4 mg/kg body weight), curcumin (80 mg/kg body weight), curcumin post-treatment (CuSO4 for 15 days followed by curcumin for the next 15 days) and curcumin co-treatment (CuSO4 plus curcumin for 30 days). All rats were treated orally by stomach tube for 30 days/once a day. Changes were observed in hepatic marker enzymes such as: aspartate aminotransferase (AST), alanine transaminase-(ALT), alkaline phosphatase (ALP) and gamma-glutamyltransferase (GGT), besides the serum total protein, urea and creatinine. Concentration of liver and kidney antioxidants such as: catalase (CAT), superoxide dismutase (SOD), reduced glutathione-(GSH) and malondialdehyde (MDA) were measured. An increased in the activities of liver marker enzymes, urea, creatinine and the MDA contents were detected after exposure to CuSO4. Meanwhile, the activities of serum total protein, hepatic and renal antioxidants were decreased. Changes in all biochemical parameters were alleviated by the post-treatment and co-treatment of curcumin. Our finding suggests that the curcumin showed protective effects on CuSO4-induced hepatotoxicity and nephrotoxicity. 相似文献
19.
K. A. Faseehuddin Shakir Basavaraj Madhusudhan 《Indian journal of clinical biochemistry : IJCB》2007,22(1):117-121
Rats fed with hypercholesterolemic diet showed a significant increase in serum total—cholesterol, liver homogenate total-cholesterol,
HDL-cholesterol and changed LDL-cholesterol, and HDL/LDL ratio in comparison to control. Flaxseedchutney (FC) supplemented diet (15%, w/w) was found to be more effective in restoring lipid profile changes in rats fed with cholesterol,
(1.0%). The activities of serum marker enzymes glutamate oxaloacetate transminase (GOT), glutamate pyruvate transaminase (GPT)
and alkaline phosphatase (ALP) were elevated significantly in carbon tetrachloride induced rats. Administration of flaxseedchutney (15%, w/w) resulted in depletion of serum marker enzymes and exhibited recoupment thus showing significant hepatoprotective
effect. It was observed that flaxseedchutney supplemented diet could lower the serum cholesterol and as a potential source of antioxidants it could exert protection against
hepatotoxic damage induced by carbon tetrachloride (CCl4) in rats. 相似文献
20.
Y. K. Murali Ramesh Chandra P. S. Murthy 《Indian journal of clinical biochemistry : IJCB》2004,19(2):202-204
Water extract of dry fruits ofTerminalia chebula (Hindi-Harda, Telugu-Karakkaya) at a dose of 200 mg/kg body weight improved the glucose tolerance as indicated by 44% of
reduction in the peak blood glucose at 2nd hour in glucose tolerance test in diabetic (streptozotocin induced) rats. Treatment of diabetic rats with an initial fasting
blood glucose of 253±9.4 mg/dl daily once with the water extract (200 mg/kg) for two weeks brought down the fasting blood
glucose to 123±8.4 mg/dl which is only slightly above the normal value. These results indicate that water extract of Terminalia
chebula improves glucose tolerance and brings down fasting blood glucose in diabetic rats. 相似文献