共查询到17条相似文献，搜索用时 359 毫秒
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目的观察自体角膜缘干细胞移植联合丝裂霉素C治疗翼状胬肉的效果。方法123例171眼在手术显微镜下先行翼状胬肉切除术后,自体角膜缘干细胞移植联合丝裂霉素C。术后6-36个月随访171眼。结果术后6-15个月随访171眼,4眼复发。复发率为3％。结论常规切除翼状胬肉联合自体角膜缘干细胞移植联合丝裂霉素C能有效降低胬肉复发率。 相似文献
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目的:翼状胬肉切除术结合临近带角膜缘干细胞自体结膜移位术后防止胬肉复发的临床观察.方法:翼状胬肉常规切除后将胬肉上方(或下方)相邻带角膜缘干细胞自体结膜瓣移位于巩膜暴露区.结果:25个病例28只眼术后随访3个月-1年,有一例复发.结论:翼状胬肉切除术并临近带角膜缘干细胞自体结膜移位术可降低胬肉术后复发率. 相似文献
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目的：阐明角膜缘干细胞移植治疗翼状胬肉的疗效。方法：应用角膜缘干细胞联合结膜上皮移植治疗翼状胬肉。结果：45例45眼，痊愈42例42眼，复发3例3眼。结论：本术式是目前治疗和预防翼状胬肉复发的最佳方法之一。 相似文献
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目的探讨羊膜移植术治疗翼状胬肉手术前后的护理。方法对我院2007-2008年实施羊膜移植术的患者42例42眼实施常规护理、专科护理和密切观察病情等措施。结果42例患者中2例复发,复发率4.7%。结论有效的基础护理和专科护理可提高手术成功率,减少手术并发症的发生。 相似文献
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翼状胬肉是眼科的一种常见病,张家口地区尤其是坝上,气候干燥、接触风沙加上紫外线照射等因素,损害角膜干细胞,所以患病率较高。此病的主要治疗方法为手术治疗,按常规手术方法单纯切除胬肉,术后复发率很高,可达30%左右,而多次手术可导致睑球粘连,眼球运动受限,影响视力。我院眼 相似文献
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目的观察双眼进程不同的年龄相关性白内障患者角膜内皮细胞密度及形态学变化,探讨白内障进展程度对角膜内皮细胞的影响。方法选取视力一眼≤0．2,另一眼≥0．6年龄相关性白内障患者100例,视力≤0．2眼为A组,视力≥0．6眼为B组,分别对其双眼的角膜内皮细胞密度、细胞大小变异系数（CV）及六角形细胞比例进行检查。结果A组角膜内皮细胞密度2297．65±364．66（cells／mm^2）,变异系数（56．65±7．28）％,六角形细胞比例（35．06±5．45）％;B组角膜内皮细胞密度2570．46±340．25（cells／mm^2）,变异系数（47．59±7．03）％,六角形细胞比例（41．97±5．19）％．A,B两组比较,角膜内皮细胞密度、角膜内皮细胞大小变异系数（CV）及六角形细胞比例差异有显著性（P〈0.05）。结论随着年龄相关性白内障进展程度的加重,患者角膜内皮细胞密度降低、细胞大小变异系数增大、六角形细胞比例减少,显示角膜内皮细胞的损伤凋亡可能与白内障的进展有相关性。 相似文献
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探讨新鲜羊膜移植治疗眼表化学性烧伤的临床疗效。方法收集我院化学性烧伤56例(72眼)行新鲜羊膜移植覆盖角膜结膜治疗,随访半年-2年。术后观察视力、角膜情况、植片情况,并发症。 相似文献
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目的：观察脱细胞羊膜（HAM）与小肠黏膜下层（SIS）促进大鼠皮肤缺损修复过程中血管形成的作用。方法：SD大鼠24只,在两侧背部各做1个直径为1．8cm圆形全层皮肤缺损。创面随机分为A组、B组和c组。A组HAM覆盖,B组SIS覆盖,C组纱布覆盖。在2周时处死动物取材,HE染色观察皮肤缺损修复情况。免疫组织化学染色检测VEGF,RT—PCR检测VEGF mRNA的表达。结果：A组、B组愈合较好。C组愈合较差。免疫组织化学染色显示,A、B组VEGF阳性细胞显著多于C组,其中A组最多;RT-PCR结果显示,A、B组比C组表达更多的VEGF mRNA。其中A组最多,差异有统计学意义（P〈0．05）。结论：HAM和SIS均能上调皮肤创面组织中VEGF mRNA的表达、增加VEGF的分泌,其中HAM具有更强的促进皮肤缺损修复过程中血管形成作用。 相似文献
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Objective： This study is aimed at describing the clinical outcome of amniotic membrane transplantation for exposure of porous sphere implants. Methods： A retrospective review of consecutive cases of porous sphere orbital implant exposure was carried out. Eight cases were presented between May 2004 and Oct. 2006 （5 males, 3 females; mean age 44.5 years）. Six had enucleation and two had evisceration. Exposure occurred in two primary and six secondary. Orbital implant diameter was 22 mm in seven cases and 20 mm in one case. Six patients are with hydroxyapatite and two with high-density porous polyethylene （Medpor） orbital implants. The mean time from implantation to exposure was 1.1 months （range 0.8-2 months）. All patients required surgical intervention. Results： The time of follow-up ranged from 3.0 to 28.0 months （mean 16.5 months）. Amniotic membrane grafting successfully closed the defect without re-exposure in all of these patients. The grafts were left bare with a mean time to conjunctiva of about 1 month （range 0.8-1.5 months）. Conclusion： Exposed porous sphere implants were treated successfully with amniotic membrane graft in all of patients. The graft is easy to harvest. This technique is useful, dose not lead to prolonged socket inflammation and infection, and it is valuable application extensively. 相似文献
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Effect of basic fibroblast growth factor （bFGF） on the treatment of exposure of the orbital implants 总被引：5，自引：0，他引：5
Objective： To evaluate the efficacy and the indication of basic fibroblast growth factor （bFGF） in the treatment of exposure of orbital implants. Design： Retrospective and observational case series. Methods： We reviewed 41 patients （41 eyes） suffering exposure of orbital implants from Jan. 2000 to June 2006. The study group patients with mild exposure received combined treatment with bFGF and antibiotic drops, and while the control group patients with mild exposure were treated with antibiotic drops only. The study group patients with moderate and severe exposure received combined treatment with bFGF and antibiotic drops, and after 2 months they were subjected to amniotic membrane transplantation, while the control group patients with moderate and severe exposure underwent amniotic membrane transplantation after using antibiotic drops. Observation of the growth of conjunctival epithelium and comparison of the healing rate of the two groups. Results： The healing rates of the mild, moderate and severe exposure study group were 100% and 92.3%. The healing rates of the mild, moderate and severe exposure control group were 55.6% and 66.7% respectively. The difference of the healing rates of the mild exposure study group and the control group was significant （P=-0.033）. And the difference of the healing rates of the moderate and severe exposure study group and the control group was not significant （P=-0.167）. Conclusion： bFGF may promote obviously the healing of orbital implant exposure, particularly it can be the first choice for the treatment of mild degree exposure. For the moderate and severe cases, it can be administered before surgical repair to enhance neovascularization and will tend to increase the success rate of surgical repair. 相似文献
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目的:探讨RNA干扰T细胞mTOR表达后诱导T细胞分化对小肠移植免疫耐受的影响。方法:通过shRNA干扰T细胞mTOR表达后,诱导T细胞向Foxp3+Tregs细胞分化。对60只雄性SD大鼠施行30次异位节段小肠移植,随机分为A组(mTOR-shRNA转染组)、B组(mTOR抑制剂RAD001干预)和C组(移植对照组、注射生理盐水)。观察小肠移植后受体大鼠的体重变化、生存时间及移植小肠病理切片评估排斥反应程度。结果:构建的mTOR-shRNA质粒表达载体在体外能有效地抑制大鼠骨髓细胞mTOR基因mRNA和蛋白的表达。RNA干扰大鼠T细胞mTOR基因可调节T细胞的定向分化,Tregs细胞增多,而Th17细胞分化减少。抑制mTOR基因可诱导大鼠异位小肠移植的免疫耐受,减轻受体抗移植物的排斥反应,显著延长移植物的存活时间。结论:RNA干扰T细胞mTOR表达后诱导T细胞分化对小肠移植耐受的研究为mTOR抑制剂(RAD001)在移植后的临床应用提供理论和实验依据。 相似文献
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概述了神经干细胞的生物学特性、分布以及影响其分化和增殖的因素，并探讨了神经干细胞移植和基因治疗在神经系统疾病治疗中的应用. 相似文献
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探讨新鲜羊膜移植在结膜囊成形术中的应用。方法 回顾、分析、总结采用新鲜羊膜移植行结膜囊成形术治疗结膜囊狭窄患53例。结果 一次手术成功装配义眼41例(77．34％)，并发症12例(22．66％)。结论 采用新鲜羊膜移植行结膜囊成形术，效果颇佳。 相似文献
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Xiao-zhou Mou Jian Lin Jin-yang Chen Yi-fei Li Xiao-xing Wu Bing-yu Xiang Cai-yun Li Ju-ming Ma Charlie Xiang 《Journal of Zhejiang University. Science. B》2013,14(11):961-972
Orthotopic liver transplantation (OLT) is the only proven effective treatment for both end-stage and metabolic liver diseases. Hepatocyte transplantation is a promising alternative for OLT, but the lack of available donor livers has hampered its clinical application. Hepatocyte-like cells (HLCs) differentiated from many multi-potential stem cells can help repair damaged liver tissue. Yet almost suitable cells currently identified for human use are difficult to harvest and involve invasive procedures. Recently, a novel mesenchymal stem cell derived from human menstrual blood (MenSC) has been discovered and obtained easily and repeatedly. In this study, we examined whether the MenSCs are able to differentiate into functional HLCs in vitro. After three weeks of incubation in hepatogenic differentiation medium containing hepatocyte growth factor (HGF), fibroblast growth factor-4 (FGF-4), and oncostain M (OSM), cuboidal HLCs were observed, and cells also expressed hepatocyte-specific marker genes including albumin (ALB), α-fetoprotein (AFP), cytokeratin 18/19 (CK18/19), and cytochrome P450 1A1/3A4 (CYP1A1/3A4). Differentiated cells further demonstrated in vitro mature hepatocyte functions such as urea synthesis, glycogen storage, and indocyanine green (ICG) uptake. After intrasplenic transplantation into mice with 2/3 partial hepatectomy, the MenSC-derived HLCs were detected in recipient livers and expressed human ALB protein. We also showed that MenSC-derived HLC transplantation could restore the serum ALB level and significantly suppressed transaminase activity of liver injury animals. In conclusion, MenSCs may serve as an ideal, easily accessible source of material for tissue engineering and cell therapy of liver tissues. 相似文献
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目的:对四肢主要血管损伤的诊治进行性回顾性分析。方法:对1994年￣2003年收治四肢血管损伤行自体血管和人造血管移植并得到随访的44例病例进行总结,分析其疗效及影响因素。结果:截肢3例,肢体缺血挛缩6例。神经功能恢复不完全7例。其余移植血管均通畅,远端肢体血循环良好。结论:血管移植治疗血管损伤保肢成功率高;血管移植应有明确指征,尽量采用自体静脉移植,必要时可使用人造血管;早诊断是前提、快救治是关键;须注意积极防治血管感染。 相似文献