共查询到19条相似文献，搜索用时 218 毫秒
1.
目的:探讨刺五加注射液对人宫颈癌细胞株Hela体外增殖的抑制作用。方法:采用MTT法测定了不同浓度的刺五加注射液对人宫颈癌细胞株Hela的体外抑制作用,倒置显微镜观察细胞形态变化。结果:刺五加注射液浓度越高对Hela细胞抑制作用越好,作用时间越长抑制效果越强,量效关系和时效关系良好;形态学观察到细胞凋亡。结论:刺五加注射液对人宫颈癌细胞株Hela的细胞增殖有较强的抑制作用。 相似文献
2.
目的:观察益气活血中药对肺癌细胞株体外生长抑制及E-CD表达的影响。方法:采用细胞计数、S-P免疫组织化学、图像分析等方法,观察人小细胞肺癌细胞株NC-H446在益气活血中药、全反式维甲酸(ATRA)处理不同时间后细胞生长状况,E-CD表达的影响。结果:在两干预因素下细胞生长抑制,形态改变。中药干预下,NC-H446细胞株E-CD阳性表达率分别由原来的12±8％提高到34±9％,IOD分别由78.5±16.9增加到200.3±21.7,前后相比具有明显差异(P<0.01);中药与ARTA对NC-H446的生长及E-CD表达的影响相比无明显差异(P>0.05)。结论:益气活血中药能明显抑制NC-H446生长,能促进E-CD的正常表达,减弱肿瘤细胞的侵袭转移能力,益气活血中药在上述作用方面可代替ATRA。 相似文献
3.
目的:观察鹿羊乳癖康颗粒对家兔乳腺增生病的影响,为临床实验提供理论依据。方法:用雌性健康家兔制作兔乳腺增生病动物模型,测定兔血清中雌二醇含量的变化、兔乳腺重量及光镜下兔乳腺组织的改变,评价鹿羊乳癖康颗粒调节激素等的治疗作用。结果:鹿羊乳癖康颗粒各组用药后家兔血清雌二醇含量均低于模型组,且低、中、高剂量组对降低实验家兔血清雌二醇含量有较好的剂量效应依赖关系。中、高剂量组与模型组比较有非常显著性差异(P<0.01),高剂量组降低达51.01%。乳腺重量、直径、小叶数量和组织病理观察结果表明,不同剂量鹿羊乳癖康颗粒对家兔乳腺增生均有明显的抑制作用。结论:鹿羊乳癖康颗粒对实验性家兔乳腺增生病有明显的治疗效果,可抑制家兔乳腺组织增生,且具有明显的量效依赖关系。其对家兔乳腺增生的抑制作用优于阳性对照药乳疾灵颗粒。 相似文献
4.
目的:通过端粒酶的数量变化来探讨瑞香狼毒提取物对肺癌细胞NCI-H446[H446]的抗肿瘤作用。方法:体外培养肺癌细胞NCI-H446[H446]细胞株,以不同浓度的瑞香狼毒提取物作用24h后,用荧光标记的方法定量检测端粒酶。结果:瑞香狼毒提取物作用人肺癌细胞提取物NCI-H446[H446]后,端粒酶的数量明显减少并呈现与剂量的正相关性。结论:瑞香狼毒提取物对肺癌细胞NCI-H446[H446]具有明显的抗肿瘤作用。 相似文献
5.
目的:研究秦艽多糖的体外抗肿瘤活性。方法:采用MTT法研究秦艽多糖在不同浓度和时间条件下,对人肝癌细胞SMMC7402、人胃癌细胞BGC-803、人肺癌细胞A549、人肾癌细胞HEK293的抑制作用。结果:秦艽多糖对人肝癌细胞SMMC7402、人肺癌细胞A549抑制作用明显,但对人胃癌细胞BGC-803、人肾癌细胞HEK293的抑制作用较弱;秦艽多糖对4种肿瘤细胞的抑制作用表现出一定的量效关系及时效关系。结论:秦艽多糖具有较好的体外抗肿瘤活性。 相似文献
6.
目的:探讨新城疫病毒(Newcastle disease virus,NDV)体外对S180肉瘤细胞(简称S180)生长有无抑制和诱导细胞凋亡作用。方法:取培养至对数生长期的S180细胞浓度为5×104/mL和1×106/mL分别接种于96孔板和50mL培养瓶中,加入不同浓度的NDV(1∶20、1∶10、1∶5)作用24h,分别用MTT法检测NDV对S180瘤细胞的生长抑制作用,荧光显微镜和琼脂糖凝胶电泳检测细胞凋亡。结果:经上述不同浓度的NDV作用24h,对S180细胞生长的抑制率依次为10%、26%、39%,各实验组吸光度值(A490)与对照组比较差异均有统计学意义(P<0.05)。荧光显微镜观察实验组培养的S180瘤细胞,出现细胞核固缩及凋亡小体,琼脂糖凝胶电泳见DNA梯形条带。结论:NDV对体外培养S180细胞增殖有明显抑制作用,可诱导S180细胞凋亡。 相似文献
7.
目的:研究地塞米松对胎鼠腭突中嵴上皮(Medial Edge Epithelium,MEE)细胞凋亡、增殖和分化的影响,以探讨地塞米松引起腭裂畸形的发病机制.方法:以醋酸地塞米松诱导胎鼠腭裂畸形,应用免疫组化法和图像分析研究MEE细胞中 bcl-2和p53基因在不同时期中实验组与对照组的表达情况.结果:实验组在GD13.5～16.5(GD,gestation day)MEE细胞的bcl-2 与p53表达总体维持增高趋势;GD13.5 MEE细胞的bcl-2 与p53表达实验组与对照组均无明显差别;GD14.5 MEE细胞的bcl-2与p5表达实验组明显多于对照组(P<0.01).结论:地塞米松诱发腭裂出现MEE细胞凋亡减少,通过改变MEE细胞中bcl-2和p53基因的表达而实现对MEE细胞增殖与凋亡的调控是地塞米松引起腭裂可能机制之一. 相似文献
8.
目的:观察姜黄素对人乳腺癌MCF-7细胞(简称MCF-7细胞)凋亡的诱导作用.方法:不同浓度姜黄素(0.048,0.056,0.064,0.072,0.080μmol/L)作用于MCF-7细胞24h,采用CCK-8法检测其对MCF-7细胞的生长抑制作用;琼脂糖凝胶电泳检测细胞凋亡结果.结果:经上述不同浓度姜黄素诱导24h后,MCF-7细胞生长抑制率分别为17%、28%、48%、50%及55%;琼脂糖凝胶电泳显示出DNA梯形条带.结论:姜黄素对体外培养MCF-7细胞的增殖有明显的抑制作用并可诱导其细胞凋亡. 相似文献
9.
鞣花酸对肿瘤细胞增殖抑制和诱导凋亡作用的初步研究 总被引：2，自引：0，他引：2
目的：探讨鞣花酸（ellagic acid）对肿瘤细胞的生长抑制作用及其机制.方法：用鞣花酸处理体外培养的肿瘤细胞,用细胞增殖试验（MTT法）、克隆形成试验研究ELA对肿瘤细胞的增殖抑制作用,Brdu掺入试验、流式细胞术检测ELA对肿瘤细胞DNA合成、凋亡和细胞周期的影响.结果：用ELA处理后,肿瘤细胞增殖活性降低（p〈0.05）,克隆形成下降（p〈0.005）,Brdu标记指数降低（p〈0.05）,细胞周期分布改变,凋亡指数升高（p〈0.01）,G0/G1期细胞数增加（p〈0.01）,S期细胞数减少（p〈0.01）,在一定剂量内对正常细胞无明显影响.结论：ELA对所试肿瘤细胞的增殖有显著地抑制作用,其作用机理可能与抑制肿瘤细胞DNA合成,诱导肿瘤细胞凋亡和细胞周期阻滞有关. 相似文献
10.
商南泉茗茶多糖的体外抗肿瘤活性 总被引：2，自引：0，他引：2
为了研究商南泉茗茶多糖体外抑瘤作用,采用MTT法检测其作用于结肠癌、肺癌、乳腺癌等肿瘤细胞系后的细胞存活率、LDH法检测细胞毒性、流式细胞术分析细胞周期和细胞凋亡情况。结果表明,商南泉茗茶多糖对结肠癌、肺腺癌、乳腺癌等肿瘤细胞系均有不同程度的生长抑制作用,其中对乳腺癌MCF-7细胞的增殖抑制作用最强,结肠癌次之,肺癌最弱。进一步研究表明,商南泉茗茶多糖能够使MCF-7细胞的生长周期阻滞于G0/G1期,并且有明显的诱导MCF-7细胞凋亡作用。商洛绿茶茶多糖具有明显的体外抗肿瘤活性。 相似文献
11.
This study constitutes a first step towards the elaboration of a method for intervening in the processes of solving concrete mathematical problems, based on the processing of expressions often included in the statement of addition and multiplication problems presented to second-cycle elementary students (ages 9 to 12). Forty-eight students took part in the experiment: the 29 subjects of the experimental group were divided into small groups of about 5 subjects in each and had to perform various learning exercises. The aim of these exercises was essentially to improve skills in analyzing and processing certain expressions frequently included in the statements of addition and multiplication problems. The effect of these learning exercises on problem-solving was evaluated by a problem-solving pre-test and post-test presented to the subjects in both groups. The learning exercises helped significantly to improve the problem-solving performance of subjects in the experimental group. Analysis of the verbal productions of the subjects in this group during the learning exercises revealed certain factors related to the effectiveness of the method of intervention proposed in this research. 相似文献
12.
目的：探讨不同胃粘膜病变组织中bax基因蛋白、诱导型NO合酶（iNOS）的表达及其与幽门螺杆菌（HP）感染相关性。方法：选取我院2002～2004年102例胃粘膜组织标本及患者血清。其中慢性胃炎15例,异型增生22例,胃癌65例。应用免疫组化检测上述组织的bax、iNOS表达,应用组织切片革兰染色和酶联免疫法分别检测组织中的HP及血清HP抗体。结果：在慢性胃炎、异型增生、胃癌组织中HP的检出率分别为：7/15（46.7%）、15/22（68.2%）34/65（52.3%）。且上述各组中bax、iNOS的表达与HP感染存在正相关性。不同胃粘膜病变组织中,HP阳性组bax、iNOS阳性表达率显著高于HP阴性组。结论：bax、iNOS的表达与HP感染密切相关,HP可能通过NO的诱导及bax调控使细胞增值加速和凋亡异常而参与胃癌的发生、发展过程。 相似文献
13.
探讨He—Ne激光幅照诱导肿瘤细胞凋亡而抑制肿瘤的生长。采用He—Ne激光处理HL-60细胞,流式细胞仪检测DNA含量及细胞凋亡百分率。发现在57．24J／cm^2的He—Ne激光作用下,HL-60细胞的凋亡百分率为12．83％。同时细胞周期发生改变,G1期减少,S期降低,G2期增加。结果表明在一定剂量He—Ne激光照射下,导致细胞DNA断裂,诱导HL-60细胞凋亡并抑制肿瘤细胞生长。 相似文献
14.
Objective: To construct a PC12 cell strain with neuronal differentiation, and observe the apoptosis and pro-liferation activity effects induced these cells by Amyloid beta-Protein (Aβ-43). Methods: 1) PC12 cells in logarithmicgrowth phase were subcultured for 24 h. After the culture fluid was changed, the cells were treated with Rat-β-NGF andcultured for 9 days. 2) Neuronal differentiation of PC12 cells in logarithmic growth phase were divided into four groups:control group (0), experimental group (1), experimental group (2) and experimental group (3). The concentrations of Aβ inthe four groups were 0 μmol/L, 1.25 μ mol/L, 2.5 μ mol/L and 5 μmol/L, respectively. The cells were harvested at 24, 48 and72 h later and stained with AnnexinV-FITC/PI after centrifugation and washing. Then flow cytometry was conducted toexamine the apoptosis percentage. 3) NGF-induced PC12 cells were selected and Aβ with different concentrations wasadded. The final concentrations of Aβ were 0 μmol/L, 1.25 μmol/L, 2.5 μmol/L and 5 μ mol/L, respectively. After the cellswere incubated in an atmosphere of 5% CO2 at 37 ℃ in an incubator for 72 h, the OD values were examined. Results: 1)Neuronal differentiated PC 12 cell lines were successfully established. 2) Flow cytometric examination indicated that Aβ(1.25, 2.5, and 5.0 μmol/L) could effectively induce apoptosis of neuronal-differented cells at the 24 h, 48 h and 72 h timepoints. 3) Aβ (0-5.00 μ mol/L) had no obvious effect on proliferation or restraining of the neuronal differentiation of thePC 12 cells after a 72 h interacting process. Conclusion: This investigation revealed successful neuronal differentiation of thePC12 cell strain. The induction of apoptosis of the neurocytes by various concentrations of Aβ was observed and the in-fluence of Aβ on induced proliferation of PC 12 cells by Rat-β-NGF was revealed. This study -05 provide basis for futureresearch on the molecular cure of AD and interdiction of AD evolution. 相似文献
15.
构士萍 《荆门职业技术学院学报》2007,22(9):73-75
目的:探讨中西医结合在冠心病心绞痛中的治疗效果。方法:将104例冠心病心绞痛患者随机分为治疗组和对照组,治疗组63例采用中西药结合治疗,对照组41例仅用西药治疗。结果:治疗组在临床疗效、心电图的有效率分别为85.7%和55.6%;对照组在临床疗效、心电图的有效率分别为66.7%和44.4%。两组比较,差异有显著意义(P<0.05)。结论:中西医结合治疗冠心病心绞痛疗效优于单纯西药治疗。 相似文献
16.
This study was aimed to investigate the relationship between breakfast and serum glucose, insulin and glucagon concentrations in order to establish a model breakfast appropriate for Chinese. Twenty-four volunteers were randomly assigned to four study groups: high carbohydrate breakfast, high fat and protein breakfast, the typical breakfast and fasting. Each subject had serum and urine samples collected while fasting and at 1,2 and 3.5 hours following the meal. The concentration of serum glucose, insulin and glucagon was measured. The levels of serum glucose in group A,B and C differed significantly at 1 and 2 hour after meal compared to those at fasting (P<0.05). The serum glucose in group A increased insignificantly after meal. The serum insulin levels were in group A,B and C significant different compared with control group(P<0.05).Those peaked at 1 hour after meal ,with group C rising the furthest. Compared with the fasting group, the serum glucagons rose and maintained the increase after breakfast in group A,B and C (P<0.05).The data suggested that various diets with different calorigenic amounts increased hormone concentration to various extents. We found that a breakfast rich in carbohydrates could maintain proper blood glucose level. 相似文献
17.
目的:研究新城疫病毒(NDV)感染对小鼠肉瘤S180细胞p53蛋白的表达及细胞周期的影响。方法:通过NDV体外感染小鼠肉瘤S180细胞,经倒置显微镜观察细胞形态变化,噻唑蓝(MTT)比色法检测小鼠肉瘤S180细胞的增殖状况;经流式细胞仪分析检测NDV体内感染后荷瘤鼠腹水中S180细胞分裂周期各时相的变化、细胞凋亡情况及细胞表面p53蛋白的表达情况。结果:NDV体内、外感染对小鼠肉瘤S180细胞的杀伤作用明显,NDV体内感染后荷瘤鼠腹水中S180细胞高表达p53蛋白,细胞凋亡率增加,G2/S期细胞减少,增殖指数(PI)降低,与阴性对照组比较有显著性差异(P<0.05)。结论:NDV感染可增强小鼠肉瘤S180细胞p53蛋白的表达,影响其细胞周期,诱导其凋亡且有较强的杀瘤作用。 相似文献
18.
目的:观察中药"病毒克"颗粒剂对人工感染鸡新城疫病防治的肝功指标的影响.方法:试验用75只1日龄罗曼鸡人工感染NDV后,分每千克体重给"病毒克"颗粒剂0.5g、1g治疗组和0.5g预防组,并设阳性对照组和健康对照组,测定各组肝功能指标.结果:血清总蛋白(TP)0.5 g治疗组极显著高于阳性对照组(P＜0.01);白蛋白各治疗组极显著高于阳性对照组,预防组显著高于阳性对照组;r-谷氨酰转移酶(GGT)和胆固醇(CHO)阳性对照组显著低于治疗组;乳酸脱氢酶(HDBH)阳性对照组显著高于各治疗组和预防组等.结论:中药"病毒克"颗粒剂对肝功能有较好的保护作用. 相似文献
19.
罗放华 《湖南城市学院学报》2011,32(6):55-59
基层统计市场主导模式是由政府规范、市场主导、中介执业的基层统计生产管理模式。它是基层统计生产方式和管理方式的重大变革,对于完善我国统计管理理论,推动基层统计制度、体制机制创新具有深远的理论意义,同时对于稳固统计基础,提高宏观统计数据质量和政府统计公信力都有十分重要的现实意义。 相似文献