共查询到20条相似文献，搜索用时 15 毫秒
1.
A. Akbarzadeh D. Noruzian Sh. Jamshidi A. Farhangi M. R. Mehrabi B. Lame Rad M. Mofidian A. Allahverdi 《Indian journal of clinical biochemistry : IJCB》2007,22(1):71-76
Insulin injection is the main way to combat against insulin-dependent diabetes mellitus effects. Today in some laboratories
in the world, the investigators are trying to find some treatments for this disease with insulin-secreting pancreatic islet
cells transplantation. Donor tissue in each step of work was prepared from 36 adult male wistar Rats weighted 250–300 grams
(75–90 days). Transplantation was done in rats after 2–4 weeks induction of diabetes with 60mg/kg of streptozotocin injection
by intravenous method. Encapsulation of pancreatic islet cells allows for transplantation in the absence of immunosuppression.
This technique that is called “immunoisolation” is based on the principle that transplanted tissue is protected for the host
immune system by an artificial or natural membrane. In this study, the levels of insulin, C-peptide and glucose in diabetic
rats have been reached to normal range as compared to un-diabetic rats in 20 days after transplantation of islet cells, so
that testis is immunoisolated place for islet cells transplantation. Inside the testis subcutaneously and intrapretoneally
implantation of pure islet cells graft, that is a natural immunoisolation method, rapidly and permanently normalized the diabetic
state of streptozocin-administered animals. 相似文献
2.
产业技术选择是城市发展中一项重要的决策内容。传统产业技术选择方法,诸如德尔菲法、层次分析法等,面临着技术分属领域跨度大,颗粒度大小难界定,以及专家主观判断依赖性大的问题。本研究旨在利用知识网络理论,结合文献计量的量化方法,打破传统产业技术选择的局限,帮助城市识别所需的产业技术。本研究以北京的产业技术选择为例,从梳理国家研究机构发布的技术预测结果为起点,筛选与北京城市发展相关度高的产业技术,并结合知识网络理论,量化产业技术在知识网络中的特征参数,并探讨整体网络参数对产业技术选择与政策设计的影响,由此作为城市发展规划中产业选择以及后续产业政策制定的重要参考依据。研究认为,基于知识网络基础的量化方法,在一定程度上解决了现有决策方法偏重定性分析的问题,为城市产业技术选择提供了一种具有科学依据、能够量化评价的分类方法。 相似文献
3.
H. Ravikumar K. S. Devaraju K. Taranath Shetty 《Indian journal of clinical biochemistry : IJCB》2008,23(2):117-122
Currently available method(s) for assaying pyrroline-5-carboxylate (P5C), an important intermediate metabolite of ornithine,
proline and glutamate metabolic pathways, are cumbersome or not sensitive enough for microanalysis. The present study involving
the synthesis of P5C followed by purity check, molecular mass (amu =113.1) determination by mass spectrometry and spectral
characterization of P5C-ninhydrin derivative (λ max: 510 nm) confirmed the authenticity of the preparation. Studies on the
effect of pH on spectral characteristics of P5C ninhydrin derivative demonstrated a significant change with respect to λ max
(620 nm) and several ∼ 12 fold increase in molar extinction coefficient (ε: 1.96 × 105) in alkaline conditions (pH:7.0–8.0) as compared to the reported Molar ε of 1.65 × 104 at max λ 510 nm in ethanolic solution. The modified method, with the improved sensitivity, is adopted for the assay of ornithine
amino transferase activity in WBC’s/platelets lysate(s) from human blood. 相似文献
4.
运用随机非参数数据包络分析法,选取2011年—2016年的面板数据对中国高端制造业科技创新进行了分阶段分行业的效率测度,结果表明不同行业科技创新效率差别较大,行业差异具有异质性。在效率测度基础上利用Tobit模型对各阶段科技创新效率关键影响因素进行了实证分析后,提出了提升我国高端制造业科技创新效率的对策建议。 相似文献
5.
Donatien Gatsing Ibrahim Hassan Garba Godwin I. Adoga 《Indian journal of clinical biochemistry : IJCB》2006,21(2):42-48
Recent acquisitions on the early detection and monitoring of the progression of diabetic complications (nephropathy) using
the techniques of enzymology (lysosomal enzymes) are reviewed. it appears that the kidney is the principal source of urinary
lysosomal enzymes. Urinary samples for lysosomal enzyme determination can be either 24-hour or spot-collection. The use of
synthetic substrates (4-methylumbelliferyl substrates) provides an easy, inexpensive, sensitive and highly reproducible method
of lysosomal enzyme assay. It is recommended that more than one enzyme be assayed in the process. The use of fractional enzyme
excretion (FEE) ratios is further recommended. The urinary lysosomal glycosidases investigated and found to be of particular
diagnostic value in the early detection of diabetic nephropathy include N-acetyl-β-D-glucosaminidase (β-hexosaminidase, NAG),
β-glucuronidase and β-galactosidase, with NAG being the most useful indicator. Urinary NAG can be used in monitoring the progression
of diabetic nephropathy. The fluorimetric assay of lysosomal glycosidases is particuarly recommended in developing countries
since it is simple, sensitive and inexpensive. 相似文献
6.
J. J. Fleming S. Santhosh R. Selvakumar A. Jose C. E. Eapen 《Indian journal of clinical biochemistry : IJCB》2009,24(1):15-22
Serum ceruloplasmin is one of the most commonly used screening tests for Wilson’s disease. However immunological assays for
ceruloplasmin are not recommended for diagnosis and management of Wilson’s disease through calculation of free copper index.
Enzymatic methods using non-physiological substrates have toxicity and stability problems, making them difficult to automate.
Ferroxidase assays may be a satisfactory alternative for measuring serum ceruloplasmin. The o-dianisidine hydrochloride manual
method for estimation of serum ceruloplasmin enzyme activity was compared with an automated method using the ferroxidase activity
of ceruloplasmin in measurement in a double blind study in 91 consecutive patients screened for Wilson’s disease. The o-dianisidine
and ferroxidase methods both successfully identified 7 patients with Wilson’s disease. Values for these 7 patients in the
o-dianisidine and ferroxidase methods were median 5.0 (range 0–16.0 U/L) and median 45.0 (range 4–166 U/L) respectively. There
were 7 other positive values (<62 U/L) with the o-diansidine method and 2 (<200 U/L) with the ferroxidase method, where WD
was not confirmed. ROC curves for both methods showed area under the curve of 0.998 for o-dianisidine and 0.997 for ferroxidase.
Using literature cut off values of 62 U/L and 200 U/L respectively both methods had 100% sensitivity and specificity was 91.7%
(o-dianisidine) and 97.6% (ferroxidase). For the o-dianisidine assay, specificity was improved to 98.8% using a cut off of
22.5 U/L. In the 84 persons (46 adults and 38 children) in whom the diagnosis of Wilson’s disease was not established, the
mean value for ceruloplasmin activity by the o-dianisidine and ferroxidase methods was 124.7 ± 48.7 U/L and 571.4 ± 168.1
U/L respectively. There were no significant differences between sex or age of patients (p > 0.29). In a subsequent evaluation
with 372 specimens, the Pearson correlation coefficient between the assays was 0.908, p < 0.01, slope 4.06, intercept 265.8,
with the manual assay as the x-axis. The ferroxidase assay is a suitable replacement for the o-dianisidine assay in detecting
patients with Wilson’s disease. 相似文献
7.
本文在深入研究全球科技创新中心相关研究理论的基础上,构建了全球科技创新中心评价指标体系,并运用熵权TOPSIS法对我国9个典型城市进行了实证分析,研究的结果得出我国最具有全球科技创新中心实力的城市是北京、上海和广州,并指出科技创新中心与区域经济间的协同作用。最后根据部分城市科技创新能力的短板以及其他国家的成功经验给出了政策建议。 相似文献
8.
高校大学生意识形态教育在网络时代面临着新的机遇与挑战,比较法在大学生意识形态教育中有着不可替代的重要作用,通过横向比较与纵向比较资本主义意识形态的历史和现实,解析比较作为资本主义意识形态标志的资产阶级理论如＂民主＂、＂自由＂、＂人权＂等（以资产阶级民主为例）,可以提高大学生的全面综合分析能力、逻辑思考能力、理性判断能力,从另一个方面深化他们对主流意识形态的信仰与坚持。 相似文献
9.
目前大学生在四级写作中普遍得分偏低,一个重要原因是写作课上参与度较低。 PBL教学改变了学生和教师在学习中的角色,利于学生参与,并有助于学生在解决问题的同时改进语言能力和思考能力,从而提高其在四级考试中的写作能力。 相似文献
10.
新时代创新引领发展对培育和优化创新生态系统提出新要求,需要准确客观评价区域创新生态系统健康状况。基于传统生态位适宜度模型进行改进修正,采用PCA分析法对生态因子赋权以解决评价指标共线性问题,提炼创新生态系统的开放性、多样性与协同性、演进性与成长性、可持续性等固有特征,从特性角度重构区域创新生态系统健康评价指标体系。从描述性分析和定量分析两个角度对省域和城市两个不同层次的区域创新生态系统进行健康评价。研究表明,模型与指标适合评价创新生态系统的健康度;我国区域创新生态系统健康度不平衡状况仍在加剧。培育优化创新生态系统要注重久久为功,突出新增长极,形成内生动力,促进创新行为者融合共生。 相似文献
11.
Litska Strikwerda 《Ethics and Information Technology》2012,14(2):89-97
In 2009 Dutch judges convicted several minors for theft of virtual items in the virtual worlds of online multiplayer computer
games. From a legal point of view these convictions gave rise to the question whether virtual items should count as “objects”
that can be “stolen” under criminal law. This legal question has both an ontological and a moral component. The question whether
or not virtual items count as “objects” that can be “stolen” is an ontological question. The question whether or not they
should count as such under criminal law is of a moral nature. The purpose of this paper is to answer both the ontological
question and the moral question underlying the legal question. 相似文献
12.
Ganesa Yogeeswaran Charles E. Jebaraj Venkataraman Sriram 《Indian journal of clinical biochemistry : IJCB》1999,14(2):220-228
A comparison of the absorbance, enzyme/substrate concentration, reaction efficiency and sensitivity has been made for enzyme-based
clinical chemistry assays, using a conventional colorimeter versus a strip-microwell reader, in order to establish the value
of ultra-microchemical procedure, with reaction volume 87 μl (light path length=0.25 cm). By utilizing commercial kits available
for the quantitation of serum glucose, cholesterol and triglycerides, it has been established that the micro method is highly
cost effective (9–30 fold), reproducible and sensitive. Comparison of blood drawn by a finger prick (capillary) and venipuncture
for normal and pathological specimens show reproducibility between different laboratory technologists and in reference with
the values reported by an accredited reference laboratory. Since the micro method uses very little serum, it is most suitable
for analyses of small smaples, from large population-based field trials. However, the assay range has to be titrated for each
commercial kit to establish the enzyme/substrate equivalence. 相似文献
13.
M. A. Qadar Pasha R. B. Ram M. D. Gupta 《Indian journal of clinical biochemistry : IJCB》2000,15(1):29-35
Determination of ammonia level in blood is important, especially in the diagnosis of hepatic disorders. An indigenously purified
enzyme was used in the standardisation of the assay. The assay is a two reagent system, requires five minutes for completion
and can be performed at temperature between 25–27°C. Performance of the assay was assessed by linearity, imprecision, functional
sensitivity and interference studies. Lyophilised reagent I and reagent II were found stable for at least one year. The plasma
level of ammonia for the controls was 13.7±7.3 μMol/L, whereas for subjects of hepatic disorders, it was 69.1±32.4 μMol/L
(P<0.001). The functional sensitivity was between 2–1000 μMol/L. Within-run coefficient of variation was between 1.1–2.0%
and between-run coefficient of variation was between 1.9–3.7%. The mean recovery after dilution was 99.6%. The present method
can estimate ammonia up to 1000 μMol/L without dilution of sample. Assay time of five minute may be shortened to one minute.
This method is suited for routine clinical use in treatment of hepatic disorders. 相似文献
14.
在高等教育大众化的背景下,人才的结构性矛盾显得非常突出。为更好地选拔人才,精准面试的方法被很多企业所选用。通过对精准面试的了解和探索,积极培养学生的职业保障力、职业驱动力和职业执行力有助于学生职业能力的提升。 相似文献
15.
校企共建校内生产性实训基地是提升学生实践操作能力的重要方式,也是教学实践改革和创新的重要表现。校企共建校内生产实训基地的创建,能够使学生真正将理论与实践相结合,真正展现教学的价值和意义。本篇文章主要对校企共建校内生产性实训基地的创建进行实践探究,以期能够为学生创造一个良好的学习空间,使学生能够充分地展现自我才能,促进学生更好、更快地发展。 相似文献
16.
Kenneth Einar Himma 《Ethics and Information Technology》2007,9(4):259-272
The amount of content, both on and offline, to which people in reasonably affluent nations have access has increased to the
point that it has raised concerns that we are now suffering from a harmful condition of ‹information overload.’ Although the
phrase is being used more frequently, the concept is not yet well understood – beyond expressing the rather basic idea of
having access to more information than is good for us. This essay attempts to provide a philosophical explication of the concept
of information overload and is therefore what philosophers call ‹conceptual analysis’ – a task that, along with normative
ethical analysis, is distinctive to Anglo-American style analytic philosophy. I will begin with an analysis of the atomic
concepts expressed by the terms ‹information’ and ‹overload’ and then attempt to give a philosophical explanation of the concept
of information overload that more precisely identifies exactly what the condition amounts to. 相似文献
17.
酒店业碳排放评估与节能减排潜力研究 总被引：2，自引：1，他引：1
酒店业(Hospitality)的高速发展,面临着能耗巨大和环境保护的双重压力,要实现节能减排,必须对该行业的综合能耗进行有效合理的评估,但是目前对其研究还相对滞后。本研究根据酒店行业特点,参照IPCC推荐的国际通用方法,采用分解分析法,构建了具有酒店行业特征的综合能耗和碳排放评估模型。经过对上海等地区酒店最长达5年的第一手综合能耗数据的采集和分析,结果表明:①所跟踪的某五星级酒店连续5年年均综合能耗下降4%;显示高星级酒店通过综合技改,能使可比单位建筑能耗达到小于等于55kgce/(m2·a)的先进比拟值;②建筑时间在10~20年左右的二、三星级酒店,可比单位建筑能耗数值还远高于53kgce/(m2·a)的合理值;该酒店群在我国数量多,具有很大的节能减排潜力;③从能耗影响因素分析,酒店使用电力产生的间接碳排放是第一影响因素;从能耗的种类探究,酒店的暖通和空调占综合能耗比重最高。本研究对酒店业节能减排实施方案的制定有着前置作用,同时为该行业碳交易(MRV)的启动提供了基础性研究。 相似文献
18.
在技工教育中如何提高VB程序设计这门课程的教学效果,并找出一种与学生认知规律相适应并且能够激发学生学习兴趣的教学模式,是摆在每一位从事该课程教学的教师面前的一项重要课题。本文主要针对在讲授VB程序设计课的过程中如何实施案例教学法进行了有效的思考和探索。 相似文献
19.
随着网络的普及,沉溺其中的中学生也越来越多,对中学生的生理和心理带来很大的负面影响,为了解体育锻炼对网瘾干预的效果,采用实验法等对中学生进行干预,结果显示：体育锻炼有助于削弱中学生对网络的依赖;有助于满足中学生获得平等、尊重的基本需求;有助于从时间方面帮助中学生远离成瘾;有助于中学生人格特质的积极发展,并提出了相应的建议。 相似文献
20.
Corporate dynamic transparency: the new ICT-driven ethics? 总被引：1，自引：1，他引：0
The term “corporate transparency” is frequently used in scholarly discussions of business ethics and corporate social responsibility
(CSR); however, it remains a volatile and imprecise term, often defined incompletely as “information disclosure” accomplished
through standardized reporting. Based on the results of empirical studies of organizational behaviors, this paper identifies
a new set of managerial practices based on the use of information and communication technologies (ICT) and particularly Internet-based
tools. These practices are resulting in what can be termed “dynamic transparency.” ICT allows for an informational environment
characterized by two-way exchange between corporations and their stakeholders, which fosters a more collaborative marketplace.
It is proposed that such dynamic information sharing, conducted by means of ICT, drives organizations to display greater openness
and accountability, and more transparent operations, which benefit both the corporations and their constituents. One of the
most important outcomes that will accrue to consumers and other individuals is the “right to know,” especially about corporate
strategies and activities that might directly affect their quality of life. This paper demonstrates that dynamic transparency
is more desirable and more effective than the more common “static transparency” where firms’ information disclosure is one-way,
usually in response to government regulation. We present three ethical arguments to justify the implementation by business
firms of dynamic transparency and demonstrate that their doing so is related to CSR and to augment and complement stakeholder
engagement and dialogue. The paper concludes with a summary of the possible limits to and the problems involved in the implementation
of dynamic transparency for corporations, and suggests some strategies to counter them. 相似文献