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1.
The present paper sheds some light on a mathematical model for blood flow through stenosed arteries with axially variable peripheral layer thickness and variable slip at the wall. The model consists of a core region of suspension of all the erythrocytes assumed to be a Casson fluid and a peripheral layer of plasma as a Newtonian fluid. For such models, in literature, the peripheral layer thickness and slip velocity are assumed a priori based on experimental observations. In the present analysis, new analytic expressions for the thickness of the peripheral layer, slip velocity and core viscosity have been obtained in terms of measurable quantities (flow rate (Q), centerline velocity (U), pressure gradient (−dp/dz), plasma viscosity (μp) and yield stress (θ)). Using the experimental values of Q, U, (−dp/dz), μp and θ, the values of the peripheral layer thickness, core viscosity, and slip velocity at the wall have been computed. The theoretically obtained peripheral layer thickness has been compared with its experimental value. It is found that the agreement between the two is very good (error<1.4%). Further, a comparison between theoretical and experimental values of core viscosity is made and it is observed that the error between the two becomes 3.7465% in the case of two-layered model (Casson-Newtonian) for tube diameter 40 μm. The analysis developed here could be used to determine the more accurate values of the apparent viscosity of blood, agreeability, rigidity and deformability of red cells. This information of blood could be useful in the development of new diagnosis tools for many diseases.  相似文献   

2.
We propose a blood separation microfluidic device suitable for point-of-care (POC) applications. By utilizing the high gas permeability of polydimethylsiloxane (PDMS) and phaseguide structures, a simple blood separation device is presented. The device consists of two main parts. A separation chamber with the phaseguide structures, where a sample inlet, a tape-sealed outlet, and a dead-end ring channel are connected, and pneumatic chambers, in which manually operating syringes are plugged. The separation chamber and pneumatic chambers are isolated by a thin PDMS wall. By manually pulling out the plunger of the syringe, a negative pressure is instantaneously generated inside the pneumatic chamber. Due to the gas diffusion from the separation chamber to the neighboring pneumatic chamber through the thin permeable PDMS wall, low pressure can be generated, and then the whole blood at the sample inlets starts to be drawn into the separation chamber and separated through the phaseguide structures. Reversely, after removing the tape at the outlet and manually pushing in the plunger of the syringe, a positive pressure will be created which will cause the air to diffuse back into the ring channel, and therefore allow the separated plasma to be recovered at the outlet on demand. In this paper, we focused on the study of the plasma separation and associated design parameters, such as the PDMS wall thickness, the air permeable overlap area between the separation and pneumatic chambers, and the geometry of the phaseguides. The device required only 2 μl of whole blood but yielding approximately 0.38 μl of separated plasma within 12 min. Without any of the requirements of sophisticated equipment or dilution techniques, we can not only separate the plasma from the whole blood for on-chip analysis but also can push out only the separated plasma to the outlet for off-chip analysis.  相似文献   

3.
Cheung P  Toda-Peters K  Shen AQ 《Biomicrofluidics》2012,6(2):26501-2650112
In this paper, we present a simple procedure to incorporate commercially available external pressure transducers into existing microfluidic devices, to monitor pressure-drop in real-time, with minimal design modifications to pre-existing channel designs. We focus on the detailed fabrication steps and assembly to make the process straightforward and robust. The work presented here will benefit those interested in adding pressure drop measurements in polydimethylsiloxane (PDMS) based microchannels without having to modify existing channel designs or requiring additional fabrication steps. By using three different devices with varying aspect ratio channels (wh0, width/depth), we demonstrate that our approach can easily be adapted into existing channel designs inexpensively. Furthermore, our approach can achieve steady state measurements within a matter of minutes (depending on the fluid) and can easily be used to investigate dynamic pressure drops. In order to validate the accuracy of the measured pressure drops within the three different aspect ratio devices, we compared measured pressure drops of de-ionized water and a 50 wt. % glycerol aqueous solution to four different theoretical expressions. Due to the deformability of PDMS, measured pressure drops were smaller than those predicted by the rigid channel theories (plate and rectangular). Modification of the rigid channel theories with a deformability parameter α provided better fits to the measured data. The elastic rectangular expression developed in this paper does not have a geometric restriction and is better suited for microchannels with a wider range of aspect ratios.  相似文献   

4.
Understanding the mechanical properties of optically transparent polydimethylsiloxane (PDMS) microchannels was essential to the design of polymer-based microdevices. In this experiment, PDMS microchannels were filled with a 100 μM solution of rhodamine 6G dye at very low Reynolds numbers (∼10−3). The deformation of PDMS microchannels created by pressure-driven flow was investigated by fluorescence microscopy and quantified the deformation by the linear relationship between dye layer thickness and intensity. A line scan across the channel determined the microchannel deformation at several channel positions. Scaling analysis widely used to justify PDMS bulging approximation was allowed when the applied flow rate was as high as 2.0 μl/min. The three physical parameters (i.e., flow rate, PDMS wall thickness, and mixing ratio) and the design parameter (i.e., channel aspect ratio = channel height/channel width) were considered as critical parameters and provided the different features of pressure distributions within polymer-based microchannel devices. The investigations of the four parameters performed on flexible materials were carried out by comparison of experiment and finite element method (FEM) results. The measured Young''s modulus from PDMS tensile test specimens at various circumstances provided reliable results for the finite element method. A thin channel wall, less cross-linker, high flow rate, and low aspect ratio microchannel were inclined to have a significant PDMS bulging. Among them, various mixing ratios related to material property and aspect ratios were one of the significant factors to determine PDMS bulging properties. The measured deformations were larger than the numerical simulation but were within corresponding values predicted by the finite element method in most cases.  相似文献   

5.
Ordinary and thermal diffusion of moisture in activated alumina are investigated using a new diffusion cell design and scheme of analysis reported earlier. The specific form of the mass flux equation has a pronounced effect on the magnitude of the associated thermal diffusion ratio. In the case of activated alumina-moist air, if a partial pressure gradient is used, then the thermal diffusion term is small if not zero.Free, Knudsen and surface diffusion all play a part in the diffusion through activated alumina. However, surface diffusion makes the major contribution and for this reason the model in this case can be simplified to a two parameter surface model.The activation energy for surface diffusion is constant and is approximately equal to the mean isosteric heat of absorption. In addition, mean pore radius, turtuosity, and other physical constants are computed from the least square fit of experimental data. Furthermore, the model is theoretically consistent over the entire concentration range (0≦ CA ≦ CAsat).A new fact about activated alumina (Grade F1) it that it does not transfer moisture in a nonisothermal condition so long as the partial pressures of moisture on the two sides of the pellet are the same. There appears to be no previous report of this fact in the periodical literature.  相似文献   

6.
The confined flow of red blood cells (RBCs) in microvasculature is essential for oxygen delivery to body tissues and has been extensively investigated in the literature, both in vivo and in vitro. One of the main problems still open in microcirculation is that flow resistance in microcapillaries in vivo is higher than that in vitro. This discrepancy has been attributed to the glycocalyx, a macromolecular layer lining the inner walls of vessels in vivo, but no direct experimental evidence of this hypothesis has been provided so far. Here, we investigate the flow behavior of RBCs in glass microcapillaries coated with a polymer brush (referred to as “hairy” microcapillaries as opposed to “bare” ones with no coating), an experimental model system of the glycocalyx. By high-speed microscopy imaging and image analysis, a velocity reduction of RBCs flowing in hairy microcapillaries as compared to bare ones is indeed found at the same pressure drop. Interestingly, such slowing down is larger than expected from lumen reduction due to the polymer brush and displays an on-off trend with a threshold around 70 nm of polymer brush dry thickness. Above this threshold, the presence of the polymer brush is associated with an increased RBC deformation, and RBC velocity is independent on polymer brush thickness (at the same pressure drop). In conclusion, this work provides direct support to the hypothesis that the glycocalyx is the main factor responsible of the higher flow resistance found in microcapillaries in vivo.  相似文献   

7.
The essential content of a recent paper by the present writer comprises a comprehensive discussion of the physical bases underlying derivation of formulas for calculating the temperature distribution T, maximum temperature Tm and average temperature Ta in a toroidal electrical coil of rectangular cross section, internally generated heat and change of wire resistance with temperature being taken into account. Illustratively, the solution for the boundary value condition of constant surface temperature and uniform equivalent thermal conductivity was obtained.For the most part, however, problems that arise in practice are not encompassed in the comparatively simple boundary conditions of constant temperature. Experiment shows that in general the boundary condition is T ? T′ = ? K??n; whereof n denotes the outward drawn normal to the coil surface, K = (knh) the ratio of the equivalent thermal conductivity in the direction of n to the emissivity of the boundary surface, and T and T′ are the corresponding temperatures in the coil surface and the immediately adjacent ambient medium. Again, it frequently ensues in practice that the thermal conductivity is substantially different in the directions of the two principal axes of the cross section.In the present paper formulas for T, Tm, and Ta are obtained for electrical coils of ratio of external to internal radius greater than (roughly) two whereof (i) the thermal conductivity is different in the directions of the two principal axes of the cross section, (ii) K is different on but constant over each of the four faces of the coil, and (iii) no restriction is made as to T′ except that over each face it be expressible in a generalized Fourier series. Determination of T is posed as a boundary-value problem in the mathematical theory of heat; the formal solution of T effected by expansions in orthogonal functions; and Tm and Ta then determined through use of their known relationships with T. The resulting formulas are in the form of rapidly-converging singly-infinite trigonometric-hyperbolic series. Illustrative of application of these general formulas, the maximum temperatures in a coil of given dimensions subject to two different sets of surface conditions are calculated and found to be in excellent agreement with the known measured values.The just-mentioned formulas encompass practically all cases encountered in practice except those coils which do not satisfy the restriction as to ratio of radii. For these latter formulas for T, Tm, and Ta are obtained pursuant to conditions of (i) equivalent thermal conductivity different in the directions of the two principal axes of the cross section, (ii) K, and likewise T′, different on but constant over each of the four faces of the coil. These formulas are in the form of rapidly-converging singly-infinite trigonometric- Bessel function (of zero order) series: Illustratively, the maximum temperature in a coil of given dimensions is calculated and found to be in excellent agreement with the known measured value.  相似文献   

8.
Liu Y  Hartono D  Lim KM 《Biomicrofluidics》2012,6(1):12802-1280214
This paper presents a two-stream microfluidic system for transporting cells or micro-sized particles from one fluid stream to another by acoustophoresis. The two fluid streams, one being the original suspension and the other being the destination fluid, flow parallel to each other in a microchannel. Using a half-wave acoustic standing wave across the channel width, cells or particles with positive acoustic contrast factors are moved to the destination fluid where the pressure nodal line lies. By controlling the relative flow rate of the two fluid streams, the pressure nodal line can be maintained at a specific offset from the fluid interface within the destination fluid. Using this transportation method, particles or cells of different sizes and mechanical properties can be separated. The cells experiencing a larger acoustic radiation force are separated and transported from the original suspension to the destination fluid stream. The other particles or cells experiencing a smaller acoustic radiation force continue flowing in the original solution. Experiments were conducted to demonstrate the effective separation of polystyrene microbeads of different sizes (3 μm and 10 μm) and waterborne parasites (Giardia lamblia and Cryptosporidium parvum). Diffusion occurs between the two miscible fluids, but it was found to have little effects on the transport and separation process, even when the two fluids have different density and speed of sound.  相似文献   

9.
In certain arteries the flow rate of blood can now be quantified by a non-invasive technique based on a newly developed ultrasound velocity profile meter. Clinical applications of this technique indicate that a reliable mathematical model of the circulatory system would facilitate the identification of pathologic patterns of flow rate and the corresponding possible causes. A relatively simple law for the viscoelastic behavior of arterial conduits is hypothesized and its influence on the phasis variations of pressure and flow examined with the aid of the method of characteristics. To test the feasibility of this model, its predictions of the attenuation and dispersion of small sinusoidal pressure waves are compared with data obtained in animal experiments. The results show good agreement and verify the significance of taper in reducing the attenuation of waves propagating towards the periphery and conversely increasing the damping of waves moving towards the heart. Beside this the natural pressure and flow pulses generated by the heart appear to be attenuated in a realistic manner: they no longer exhibit the somewhat exaggerated peaking predicted by the theoretical model when the viscoelasticity of the vessel wall is neglected.  相似文献   

10.
Lithium titanium oxide (Li4Ti5O12, LTO), a ‘zero-strain’ anode material for lithium-ion batteries, exhibits excellent cycling performance. However, its poor conductivity highly limits its applications. Here, the structural stability and conductivity of LTO were studied using in situ high-pressure measurements and first-principles calculations. LTO underwent a pressure-induced amorphization (PIA) at 26.9 GPa. The impedance spectroscopy revealed that the conductivity of LTO improved significantly after amorphization and that the conductivity of decompressed amorphous LTO increased by an order of magnitude compared with its starting phase. Furthermore, our calculations demonstrated that the different compressibility of the LiO6 and TiO6 octahedra in the structure was crucial for the PIA. The amorphous phase promotes Li+ diffusion and enhances its ionic conductivity by providing defects for ion migration. Our results not only provide an insight into the pressure depended structural properties of a spinel-like material, but also facilitate exploration of the interplay between PIA and conductivity.  相似文献   

11.
Circulating tumor cells (CTCs) are the principal vehicle for the spread of non-hematologic cancer disease from a primary tumor, involving extravasation of CTCs across blood vessel walls, to form secondary tumors in remote organs. Herein, a polydimethylsiloxane-based microfluidic system is developed and characterized for in vitro systematic studies of organ-specific extravasation of CTCs. The system recapitulates the two major aspects of the in vivo extravasation microenvironment: local signaling chemokine gradients in a vessel with an endothelial monolayer. The parameters controlling the locally stable chemokine gradients, flow rate, and initial chemokine concentration are investigated experimentally and numerically. The microchannel surface treatment effect on the confluency and adhesion of the endothelial monolayer under applied shear flow has also been characterized experimentally. Further, the conditions for driving a suspension of CTCs through the microfluidic system are discussed while simultaneously maintaining both the local chemokine gradients and the confluent endothelial monolayer. Finally, the microfluidic system is utilized to demonstrate extravasation of MDA-MB-231 cancer cells in the presence of CXCL12 chemokine gradients. Consistent with the hypothesis of organ-specific extravasation, control experiments are presented to substantiate the observation that the MDA-MB-231 cell migration is attributed to chemotaxis rather than a random process.  相似文献   

12.
This paper aims to contribute to the literature on the long-debated relationship between market competition and firm research and development (R&D) by investigating the effect of competitive market pressure on firms’ incentives to invest in R&D. The paper shows that a firm's R&D response to competitive market pressure depends primarily on its level of technological competence or R&D productivity: firms with high levels of technological competence tend to respond aggressively (i.e., exhibit a higher level of R&D efforts) to intensifying competitive market pressure, while firms with low levels of technological competence tend to respond submissively (i.e., exhibit a lower level of R&D efforts). The differential effect of competitive market pressure on firm R&D, conditioned primarily by the level of firms’ technological competence, is empirically supported by unique firm-level data from the World Bank. Furthermore, the role of firm-specific technological competence in conditioning the R&D-competition relationship is more evident and statistically more significant for firms facing consumers whose utility is relatively more elastic to product quality than to price.  相似文献   

13.
Dielectrophoresis (DEP) has been shown to have significant potential for the characterization of cells and could become an efficient tool for rapid identification and assessment of microorganisms. The present work is focused on the trapping, characterization, and separation of two species of Cryptosporidium (C. parvum and C. muris) and Giardia lambia (G. lambia) using a microfluidic experimental setup. Cryptosporidium oocysts, which are 2-4 μm in size and nearly spherical in shape, are used for the preliminary stage of prototype development and testing. G. lambia cysts are 8–12 μm in size. In order to facilitate effective trapping, simulations were performed to study the effects of buffer conductivity and applied voltage on the flow and cell transport inside the DEP chip. Microscopic experiments were performed using the fabricated device and the real part of Clausius—Mossotti factor of the cells was estimated from critical voltages for particle trapping at the electrodes under steady fluid flow. The dielectric properties of the cell compartments (cytoplasm and membrane) were calculated based on a single shell model of the cells. The separation of C. muris and G. lambia is achieved successfully at a frequency of 10 MHz and a voltage of 3 Vpp (peak to peak voltage).  相似文献   

14.
Song W  Psaltis D 《Biomicrofluidics》2011,5(4):44110-4411011
We present a novel image-based method to measure the on-chip microfluidic pressure and flow rate simultaneously by using the integrated optofluidic membrane interferometers (OMIs). The device was constructed with two layers of structured polydimethylsiloxane (PDMS) on a glass substrate by multilayer soft lithography. The OMI consists of a flexible air-gap optical cavity which upon illumination by monochromatic light generates interference patterns that depends on the pressure. These interference patterns were captured with a microscope and analyzed by computer based on a pattern recognition algorithm. Compared with the previous techniques for pressure sensing, this method offers several advantages including low cost, simple fabrication, large dynamic range, and high sensitivity. For pressure sensing, we demonstrate a dynamic range of 0-10 psi with an accuracy of ±2% of full scale. Since multiple OMIs can be integrated into a single chip for detecting pressures at multiple locations simultaneously, we also demonstrated a microfluidic flow sensing by measuring the differential pressure along a channel. Thanks to the simple fabrication that is compatible with normal microfluidics, such OMIs can be easily integrated into other microfluidic systems for in situ fluid monitoring.  相似文献   

15.

Introduction

This pilot study aimed to investigate the use of personal protective equipment (PPE) and compliance to the code of conduct (rules defined in institutional, governmental and professional guidelines) among laboratory technicians in Croatian medical laboratories. In addition, we explored the differences in compliance between participants of different age groups, laboratory ownership and accreditation status.

Materials and methods

An anonymous and voluntary survey with 15 questions was conducted among Croatian medical laboratory technicians (N = 217). The questions were divided into two groups: demographic characteristics and the use of PPE. The questions of the second part were graded according to the Likert scale (1-4) and an overall score, shown as median and range (min-max), was calculated for each participant. Differences between the overall scores were tested for each group of participants.

Results

The majority of participants always wear protective clothes at work, 38.7% of them always wear gloves in daily routine, more than 30.0% consume food and almost half of them drink beverages at workplace. A significantly lower overall score was found for participants working in public compared to private laboratories (36 (16-40) vs. 40 (31-40), P < 0.001). There were no statistically significant differences in overall scores for participants of different age groups (P = 0.456) and laboratory accreditation status (P = 0.081).

Conclusion

A considerable percentage of laboratory technicians in Croatian medical laboratories do not comply with safety measures. Lack of compliance is observed in all personnel regardless laboratory accreditation and participants’ age. However, those working in private laboratories adhere more to the code of conduct.Key words: survey, compliance, laboratory technician, universal safety measures, total quality management, clinical laboratory services  相似文献   

16.
This paper presents a vertically positioned microfluidic system made of poly(dimethylsiloxane) (PDMS) and glass, which can be applied as a microbubble column (μBC) for biotechnological screening in suspension. In this μBC, microbubbles are produced in a cultivation chamber through an integrated nozzle structure. Thus, homogeneous suspension of biomass is achieved in the cultivation chamber without requiring additional mixing elements. Moreover, blockage due to produced carbon dioxide by the microorganisms—a problem predominant in common, horizontally positioned microbioreactors (MBRs)—is avoided, as the gas bubbles are released by buoyancy at the upper part of the microsystem. The patterned PDMS layer is based on an optimized two-lithographic process. Since the naturally hydrophobic PDMS causes problems for the sufficient production of microbubbles, a method based on polyelectrolyte multilayers is applied in order to allow continuous hydrophilization of the already bonded PDMS-glass-system. The μBC comprises various microelements, including stabilization of temperature, control of continuous bubble formation, and two optical configurations for measurement of optical density with two different sensitivities. In addition, the simple and robust application and handling of the μBC is achieved via a custom-made modular plug-in adapter. To validate the scalability from laboratory scale to microscale, and thus to demonstrate the successful application of the μBC as a screening instrument, a batch cultivation of Saccharomyces cerevisiae is performed in the μBC and compared to shake flask cultivation. Monitoring of the biomass growth in the μBC with the integrated online analytics resulted in a specific growth rate of 0.32 h−1, which is almost identical to the one achieved in the shake flask cultivation (0.31 h−1). Therefore, the validity of the μBC as an alternative screening tool compared to other conventional laboratory scale systems in bioprocess development is proven. In addition, vertically positioned microbioreactors show high potential in comparison to conventional screening tools, since they allow for high density of integrated online analytics and therefore minimize time and cost for screening and guarantee improved control and analysis of cultivation parameters.  相似文献   

17.
In this investigation we use a modified Networked Readiness Index (NRI) framework to investigate whether (1) ICT capabilities impact public value creation, and (2) if the public value is associated with the socio-economic impact of ICT capabilities. In the case of this study the construct Public Value is represented via two different proxies. In the first case we use a perception-based measure of public value (as represented by the World Government Indicators) and in the second case we use a surrogate objective measure (as represented by the Cost of Business Startup Procedures (CBSP)). We use a six-step multi-method methodology that involves Cluster Analysis, Correlation Analysis, Decision Trees Induction, Data Envelopment Analysis, Association Rules Mining, and Ordinary Least Squares regression to conduct the inquiry in the context of 26 Sub-Saharan (SSA) economies. Results of our data analysis include: 1) the set of economies with better developed ICT Capabilities are relatively more efficient in converting ICT Capabilities into Public Value than the relatively poorer economies with less developed ICT Capabilities; 2) High levels of ICT capabilities in the areas of Affordability Readiness, Skills Readiness, the Political & Regulatory Environment, and Business Usage allow for relatively more efficient generation of Public Value.  相似文献   

18.
Recently, social network has been paid more and more attention by people. Inaccurate community detection in social network can provide better product designs, accurate information recommendation and public services. Thus, the community detection (CD) algorithm based on network topology and user interests is proposed in this paper. This paper mainly includes two parts. In first part, the focused crawler algorithm is used to acquire the personal tags from the tags posted by other users. Then, the tags are selected from the tag set based on the TFIDF weighting scheme, the semantic extension of tags and the user semantic model. In addition, the tag vector of user interests is derived with the respective tag weight calculated by the improved PageRank algorithm. In second part, for detecting communities, an initial social network, which consists of the direct and unweighted edges and the vertexes with interest vectors, is constructed by considering the following/follower relationship. Furthermore, initial social network is converted into a new social network including the undirected and weighted edges. Then, the weights are calculated by the direction and the interest vectors in the initial social network and the similarity between edges is calculated by the edge weights. The communities are detected by the hierarchical clustering algorithm based on the edge-weighted similarity. Finally, the number of detected communities is detected by the partition density. Also, the extensively experimental study shows that the performance of the proposed user interest detection (PUID) algorithm is better than that of CF algorithm and TFIDF algorithm with respect to F-measure, Precision and Recall. Moreover, Precision of the proposed community detection (PCD) algorithm is improved, on average, up to 8.21% comparing with that of Newman algorithm and up to 41.17% comparing with that of CPM algorithm.  相似文献   

19.
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20.
Throughout this paper data have been presented showing that the apparent inconsistency of the reported dielectric strength behavior of insulating liquids can be satisfactorily correlated if proper consideration be given to the state of the “purity” of the liquid itself. As a result it is suggested that insulating liquids should be classified as (a) “pure,” indicating those liquids free from dissolved gases as primary “impurities”; and (b) “impure,” including those liquids which contain dissolved gas. The breakdown mechanism depends on the distinctive behavior of these two general classes. “Pure” liquid breakdown is a function of charged particle formation. In part, this may be caused by the assumption of a charge by molecular aggregates, colloidal-like in nature. In part, the charge may arise from molecular ionization by collision. The latter occurs chiefly in the voltage range immediately preceding electrical rupture and is the chief cause of “pure” liquid insulation failure. The presence of the first type of charge—that is, the existence of a difference of potential between molecular aggregates and the liquid—is chiefly responsible for the variation in the time factor to breakdown.The breakdown of “impure” liquids is a function of dissolved gas elimination. This dissolved gas is eliminated as a result of changing solubility produced (a) by electro-striction effects, or (b) by changing pressure or temperature. The presence of secondary impurities such as dust particles and fibers, acts chiefly through the effect on increasing gassing tendencies.It is suggested further that the localization of dielectric breakdown in liquids, irrespective of the type or degree of “purity,” is chiefly in the “neutral membrane” located near the electrodes and formed by the discharge of particles. Such a “neutral membrane” results in a space charge effect giving marked drop in potential and as a result promoting ionization by collision effects in “pure” liquids and electro-striction effects in “impure” liquids.  相似文献   

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