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1.
目的：评价Thinprep液基细胞学在宫颈癌筛查的准确性。方法：1881例受检者同时做宫颈常规脱落细胞涂片巴氏染色、液基标本采集和阴道镜活检．细胞学诊断采用TBS分级系统，阳性诊断包括意义不明的不典型鳞状细胞病变，所有检查均双盲进行。结果：Thinprep液基细胞学检出100％（12／12）的鳞状细胞癌；93．2％（69／74）的鳞状上皮内高度病变，其中CIN396．8％（30／31），CIN290．7％（39／43）；72．4％（92／127）的鳞状上皮内低度病变。常规脱落细胞学检出100％（12／12）的鳞状细胞癌I90．2％（66／74）的鳞状上皮内高度病变，其中CIN396．8％（30／31），CIN286．7％（36／43）；71．1％（91／127）的鳞状上皮内低度病变。Thinprep液基细胞学对鳞状细胞癌和CIN3的分级准确率分别达100％和87．1％，常规脱落细胞学涂片对鳞状细胞癌和CIN3的分级准确率分别达100％和85．0％。结论：宫颈液基细胞学检测方法较比常规脱落细胞学检测敏感性高，尤其是对鳞状上皮内高度病变。 相似文献
2.
从石斑鱼弧菌病病原创伤弧菌中提取出粗制脂多糖（LPS）加热去毒后注射免疫石斑鱼，鱼体在较短时间（7d）内快速产生体液免疫应答，应答期在56d以上．攻毒感染后，免疫鱼显示出较强的保护功能．二次免疫效果较好 相似文献
3.
陈尧顺 《宁德师专学报(自然科学版)》1995,(2)
木毒蛾（Ｌｙｍａｒｔｒｉａｘｙｌｉｎａｗｉｎｂｏｅ）是福建省沿海木麻黄防护林的重要害虫，利用木毒蛾质型多角体病毒（ＬｘＣＰＶ）防治本毒娥，防治效果达７９．７％以上．同时用木毒蛾质型多角体病毒与木毒蛾核型多角体病毒混合感染木毒蛾幼虫，死亡率为１００％．用粗提纯病毒配制成乳剂，在室温下．存放７４０天，仍保持较强感染力．用ＬｘＣＰＶ对家兔、鸽、鸡、小白鼠、金鱼等五种动物做急性毒性和亚毒性试验，试验结果以上动物无异常．用ＬＸＣＰＶ降解的病毒粒子攻击组织培养的ＦＬ，２ＢＳＢＨＫ，Ｃ６／３６细胞，未发现细胞病变和病毒增殖。 相似文献
4.
用乳糖酸红霉素（2．94g／kg）、头孢氨苄（6．19g／kg）、盐酸林可霉素（2．02g／kg）、氨苄西林钠（5．5g／kg）,3次／天,连续10天灌服小鼠,分别于灌服后第2d、4d、6d、8d、10d、12d、14d、16d对直肠和盲肠粪便中杆菌和球菌的菌落数进行测定。结果表明：灌服抗生素后杆菌菌落数量减少,球菌菌落数量增加,取代杆菌成为直肠和盲肠中的优势菌群;停药后第6d,除盐酸林可霉素组外,其它灌服组杆菌和球菌数量均可逐渐恢复。结论：乳糖酸红霉素、头孢氨苄、盐酸林可霉素及氨苄西林钠均能导致小鼠肠道菌群失调,其中盐酸林可霉素对肠道杆菌的抑制作用较明显,诱导效果较稳定和持久。 相似文献
5.
目的：评价国产新福菌素对老年非小细胞肺癌（NSCLC）的疗效和不良反应。方法：选取老年NSCLC病例81例，随机分治疗组43例给予新福菌素6μg／kg（第1～4d）。卡铂（CBP）300mg／m^2（第1d），依托泊苷（VP-16）100mg（第1～4或5d）静脉滴注；对照组38例单纯使用CBP和VP—16，剂量和方法同治疗组。21～28d为一个疗程．2～3个疗程评价疗效，随访缓解期和生存期。结果：治疗组有效率为53．49％，对照组有效率为31.58％，差异有显著性（P〈0．05）；中位缓解期分别为6和4．5个月，中位生存期分别为9和7个月．一年生存率分别为39．53％（17／43）和26．32％（10／38）。结论：新福菌素联合CBP、VP—16治疗老年NSCLC有较好的临床疗效及应用价值。 相似文献
6.
本试验测定了不同日龄青紫蓝兔对兔瘟病毒的易感性。试验选用不同日龄的青紫蓝兔54只，分成6组，9只／组，分别为30、40、50、60、90和120日龄组，分别对兔瘟脏器湿毒的敏感性进行人工感染测定。结果表明30、40、50、60、90和120日龄组青紫蓝兔对兔瘟病毒的发病率分别为33．33％、66．67％、100％、100％、100％和100％。其中30日龄和60日龄青紫蓝兔人工感染后，其发病率范围在33．33％∽100％。从试验结果中可以看出人工攻毒30∽60日龄青紫蓝兔可以被兔瘟病毒感染且发病，但不同日龄的青紫蓝兔对兔瘟病毒的感染存在有日龄上的差异。试验结果表明兔瘟病有向幼龄化发展的趋势。结果显示青紫蓝兔在40日龄左右用兔瘟组织灭活疫苗进行首次免疫，60日龄时进行二免，对兔瘟病的免疫预防具有明显保护作用。 相似文献
7.
8.
多杀性巴氏杆菌是巴氏杆菌病的病原菌。菌体短粗、呈卵园形或球形、革兰氏阴性,不形成芽胞,无鞭毛,不能运动。美兰染色,可见菌体两端浓染,呈两极染色性,新分离菌株多见有荚膜。我院动物室以架式笼养分三个饲养间共饲养家兔354只,其中供试验用青年兔140只,幼兔154只,孕兔及哺乳兔60只。于1985年5月10日至5月18日共9天的时间在 相似文献
9.
TCT、阴道镜在宫颈肿瘤筛查中的价值探讨 总被引：1，自引：0，他引：1
目的：探讨宫颈肿瘤筛查中液基细胞学检查（TCT）、阴道镜下活检联合应用的临床价值。方法：收集2002．12-2005．10月间张家口市及周边地区9250例已婚妇女进行常规细胞学检查，可疑宫颈病变3536例患者行液基细胞学检查，阴道镜检查及病理组织学检查。结果：液基细胞学筛查检出阳性涂片212例，占82．2％（212／258），其中不典型鳞状上皮细胞（ASCUS）占15．1％（32／212），鳞状上皮内低度病变（LAIL）占65．6％（144／212）；鳞状上皮内高度病变（HSIL）占79．8％（14／16），鳞状细胞癌（SCC）占100％（22／22）。阴道镜检查阳性例数为242例，占总数的93．8％（242／258）；经病理确诊为CIN及浸润癌者共197例，占TCT异常者92．9％（197／212），占阴道镜异常图象81.6％（197／242）。年龄分布上，以28～35岁发病者所占比例最大（48．5％）。结论：在宫颈病变的早期筛查中TCT联合阴道镜下活检具有重要的临床意义。 相似文献
10.
目的：了解2011年临床分离菌株对常用抗生素的耐药性。方法：采用API鉴定和ATB药敏板条及纸片扩散法对2011年临床分离1127株细菌进行药敏试验。结果：1127株细菌中革兰阴性菌占76．8％,革兰阳性菌占23．2％。革兰阴性菌中前3位为大肠埃希菌45．7％（395／865）、肺炎克雷伯菌10．6％（92／865）和铜绿假单胞菌9．0％（78／865）;革兰阳性菌中前3位为屎肠球菌27．1％（71／262）、金黄色葡萄球菌24．8％（65／262）和表皮葡萄球菌16．0％（42／262）。药敏试验结果显示泛耐药铜绿假单胞菌和不动杆菌检出率分别为19．2％（15／78）和32．8％（23／70）;MRSA和MRCNS分别占金黄色葡萄球菌和CNS的32．3％（21／65）和83．3％（40／48）。结论：定期进行耐药性监测有助于了解细菌耐药性的变迁,为临床用药提供依据。 相似文献
11.
本试验测定了不同日龄青紫蓝兔对兔瘟病毒的易感性.试验选用不同日龄的青紫蓝兔54只,分成6组,9只/组,分别为30、40、50、60、90和120日龄组,分别对兔瘟脏器湿毒的敏感性进行人工感染测定.结果表明30、40、50、60、90和120日龄组青紫蓝兔对兔瘟病毒的发病率分别为33.33%、66.67%、100%、100%、100%和100%.其中30日龄和60日龄青紫蓝兔人工感染后,其发病率范围在33.33%～100%.从试验结果中可以看出人工攻毒30～60日龄青紫蓝兔可以被兔瘟病毒感染且发病,但不同日龄的青紫蓝兔对兔瘟病毒的感染存在有日龄上的差异.试验结果表明兔瘟病有向幼龄化发展的趋势.结果显示青紫蓝兔在40日龄左右用兔瘟组织灭活疫苗进行首次免疫,60日龄时进行二免,对兔瘟病的免疫预防具有明显保护作用. 相似文献
12.
Protection of Carassius auratus Gibelio against infection by Aeromonas hydrophila using specific immunoglobulins from hen egg yolk 总被引：3，自引：0，他引：3
Specific immunoglobulin （IgY） from egg yolk against Aeromonas hydrophila was produced by immunization of White Leghorn hens with formalin-killed whole cells of A. hydrophila. ELISA test using A. hydrophila as the coating antigen revealed that the specific antibody titer started to increase in the egg yolk at the 13th day post-immunization （P/N=2.18）, reached the peak at the 56th day （P/N= 13.82）, and remained at high level until day 133 （P/N=7.03）. The antibody was purified by saturated ammonium sulphate with a recovery rate of 63.5%. The specific IgY inhibited the growth ofA. hydrophila at a concentration of 1.0 mg/ml during the 18 h incubation. Pre-treatment of polyploid gibel carps Carassius auratus Gibelio with specific IgY had a protection rate of 60% （6/10） against challenge with A. hydrophila, while none of the fishes in the control groups receiving sterile phosphate buffered saline （PBS） or non-specific IgY survived the challenge. Treatment of fishes with the specific IgY 4 h after the challenge also had lower mortality （70%, 7/10）, a 30% reduction against the control PBS or non-specific IgY groups （10/10）. These results indicate that specific IgY antibodies could be obtained easily from hens immunized with an inactivated A. hydrophila and could provide a novel alternative approach to control of diseases in fishes caused by this organism. 相似文献
13.
Lei Tian Xiao-qian Dang Chun-sheng Wang Pei Yang Chen Zhang Kun-zheng Wang 《Journal of Zhejiang University. Science. B》2013,14(5):426-437
The aim of this study is to investigate the effects and possible mechanisms of sodium ferulate (SF) on anti-apoptosis in steroid-induced femoral head osteonecrosis in rabbits. Japanese white rabbits were randomly divided into three groups (control group, treatment group, and model group), each with 24 rabbits. The model and treatment groups were first injected with an intravenous dose of horse serum, 10 ml/kg, three weeks later with an intravenous dose of 7.5 ml/kg, and two weeks later with an intramuscular dose of methylprednisolone, 45 mg/kg, three times in order to establish rabbit models of osteonecrosis. Concurrently, the treatment group was injected with intravenous doses of SF 20 mg/kg for two weeks, once per day. Three time points, Weeks 2, 4, and 8, were selected after modeling was completed. Osteonecrosis was verified by histopathology with haematoxylin-eosin (HE) staining. The apoptosis rate of osteonecrosis was observed by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. The apoptosis expressions of caspase-3 and Bcl-2 were analyzed by immunohistochemistry and Western blot. The rabbit models of osteonecrosis were successfully established and observed by HE staining. SF was effective in intervening in apoptosis and decreasing the apoptosis rate in femoral head necrosis by the immunohistochemistry and TUNEL assay (P<0.01). Western blot analysis indicated that there were statistical significances in the protein levels of caspase-3 and Bcl-2 (P<0.01). SF has a protective effect by reducing the incidence of early steroid-induced femoral head necrosis in rabbits, effectively intervening in apoptosis through decreasing caspase-3 expression and up-regulating Bcl-2 expression. 相似文献
14.
Habib M Hussain I Fang WH Rajput ZI Yang ZZ Irshad H 《Journal of Zhejiang University. Science. B》2006,7(4):320-323
In this experiment conducted to study the inactivation dynamics of infectious bursal disease virus （IBDV） by binary ethylenimine （BEI） in comparison with formalin, IBDV was isolated from the bursa of infected chickens and its confirmation was done by agar gel precipitation test. Viral suspensions were subjected to inactivation with BEI and formalin for pre-set time in- tervals. BEI was employed at concentrations of 0.001 and 0.002 mol/L while formalin was used at 0.1% and 0.2%. Sampling was done at 6, 12, 24, 36 and 48 h of incubation and samples were tested for their inactivation status in 9-day-old embryonated eggs and 3-week-old broiler chickens. IBDV was completely inactivated by 0.001 and 0.002 mol/L BEI after 36 h of incubation at 37℃, whereas formalin at 0. 1% and 0.2% concentrations inactivated IBDV in 24 h. 相似文献
15.
目的:探讨以柚皮素(C15H12O5)为母核进行结构修饰改造的系列化合物对对乙酰氨基酚(acetaminophen,APAP)诱导的L02肝细胞损伤的预防和治疗作用。方法:设立溶剂对照组、APAP模型组、APAP+化合物组和化合物自身对照组,用CCK-8法检测细胞的存活率。通过存活率的高低,比较不同化合物对APAP诱导的L02肝细胞损伤的影响。结果:化合物预给药结果显示,与APAP模型组相比,25μmol/L的化合物3,4,5,6,7,8可显著提高细胞存活率(p<0.05),而化合物1(柚皮素)和化合物2(柚皮苷)组细胞存活率无显著变化(p>0.05)。化合物后给药结果显示,与APAP模型组相比,25μmol/L的化合物3,4,5,6,7,8可显著提高细胞存活率(p<0.05),而柚皮素和柚皮苷组细胞存活率无显著变化(p>0.05)。化合物保肝活性剂量依赖性实验结果显示,与模型组(APAP)相比,化合物3、8的5μM、10μM、25μM浓度组、化合物4、5的10、25μM浓度组和化合物6、7的25μM、50μM、100μM浓度组的细胞存活率均显著提高(p<0.05)。结论:本研究中,柚皮素结构修饰产物化合物对APAP诱导的肝细胞损伤不管是损伤前预给药,还是损伤后给药均具有拮抗作用,且其拮抗作用具有剂量依赖性。实验结果表明柚皮素经过结构改造后,其修饰产物保护肝细胞的作用明显提高。 相似文献
16.
目的观察川芎嗪酯类衍生物对H2O2引起的体外培养人脐静脉内皮细胞氧化性损伤的保护作用,并初步探讨川芎嗪双酯衍生物在家兔动脉内皮氧化损伤中的保护作用。方法H2O2引起体外培养人脐静脉内皮细胞及家兔动脉内皮氧化性损伤,观察川芎嗪双酯衍生物对内皮细胞氧化损伤的保护作用。结果在各试验浓度,大多数川芎嗪双酯衍生物对H2O2引起体外培养人脐静脉内皮细胞氧化性损伤有明显的保护及促增殖作用,A6在较高浓度对家兔动脉内皮的氧化损伤有保护作用。结论川芎嗪酯类衍生物对H2O2引起的体外培养人脐静脉内皮细胞氧化性损伤有明显保护作用,其中以A15效价最高,在其作用机制进一步研究中值得注意。 相似文献
17.
Robin E. Remsburg Karen A. Armacost Charlotte Radu Richard G. Bennett 《Educational gerontology》2013,39(3-4):261-280
The purpose of this study was to determine the impact of a progressive mobility restorative program on nursing home residents' ability to perform selected activities of daily living (ADLs) and the amount of staff assistance needed to help residents complete these ADLs. A one group repeated measures design study was conducted in a 255-bed university affiliated geriatrics center. A certified nursing assistant (CNA) specially trained in restorative mobility techniques worked with residents individually for at least 15 minutes per day, five days per week to achieve their individualized mobility goals. Items on ADL Self-Performance and ADL Support Provided from the Minimum Data Set (MDS) were collected at baseline, three months, and six months. Thirty-eight of 42 residents (90%) were included in the analyses and 31 residents (82%) completed at least 25 days of restorative activities. Some residents improved their ADL self-performance at three and six months (15% and 33%, respectively) and decreased in the amount of ADL support provided at three and six months (26% and 31%, respectively). Total ADL self-performance and support provided did not change significantly over the six-month period. Most residents maintained their baseline ADL self-performance and level of ADL support provided over the six-month study. A progressive mobility restorative program implemented by a certified nursing assistant specially trained in restorative techniques resulted in most residents maintaining their baseline ADL self-performance abilities and the level of assistance provided by staff. Studies are needed to determine if these types of programs can actually improve functional abilities and decrease the level of staff assistance needed to complete ADLs or if they are effective in maintaining or delaying functional loss and staff burden. 相似文献
18.
Dario Varin Cristina Riva Crugnola Paola Molina Chiara Ripamonti 《European Journal of Psychology of Education - EJPE》1996,11(2):215-229
This study investigated how the age of entry into regular group day care may influence, in the context of other relevant variables, some short-time outcomes of this experience. In particular the study focused on behaviours expressing well-being or discomfort in the day care environment during the third year. Subjects were 129 young children who were enrolled in group day care when they were 6–29 months old. During their third year their social behaviour was evaluated by caregivers with the Day Care Adaptation Scale. Researchers observed 54 of the children in reunion with their parents. The quality of care was rated with the ITERS scale. It was found that children who started day care in the intervals 6–12 months and 18–23 months displayed more frequently difficult reunions with their mothers, and were rated as less resistant to frustration. Children who started day care from 12–17 months showed lower relational distress. Some significant relationships were found between the ratings of ITERS and the Day Care Adaptation Scale. These findings are discussed with reference to theories of attachment. 相似文献
19.
Allison Frost Michelle Bosquet Enlow Ashley J. Malin Kristin Bernard Rosalind J. Wright 《Child development》2021,92(6):e1260-e1274
This study examined associations among early adversity, diurnal cortisol, child sex, and caregiver sensitivity at age 6 months in relation to wheezing in children (47% male) followed to 30 months. Analyses included 676 mother-child dyads, 393 of whom completed an observational caregiver sensitivity measure. Participants were primarily ethnic minorities (42.7% Black, 25.4% Hispanic); 22.1% of children had ≥ 1 wheezing episode. Higher adversity was associated with increased wheeze frequency and blunted diurnal cortisol slope. The indirect effect of adversity on wheezing through cortisol slope was significant for females, but not males. Higher caregiver sensitivity was protective against wheezing for males, but not females, with high cortisol. Findings suggest complex associations among adversity, cortisol, child sex, and caregiver sensitivity in predicting wheezing. 相似文献
20.
We extracted six Hong Kong brown seaweed species with hot water for their antiviral properties. The cytotoxicity and antiviral activity of these extracts were tested by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenlytetrezolium bromide] method, cytopathic effect reduction assay, and plaque reduction assay. The antiviral effect was further determined by flow cytometric analysis. The results showed that most of these extracts inhibited the propagation of herpes simplex virus types 1 and 2 (HSV-1 and HSV-2) standard strains with very low cytotoxicity to the host cells. The extracts ofHydroclathrus clathratus and Lobophora variegata showed more potential anti-HSV activities than the extracts of the other four seaweeds. They also had moderate anti-respiratory syncytial virus (RSV) activities but could not inhibit influenza A virus. Hydroclathrus clathratus was further extracted by diluted acid and alkali and the antiviral effects of the extracts were also detected. The result showed that the hot water extract contained the main carbohydrate components that exhibited the antiviral activities against various strains of HSV, including the acyclovir-resistant strain. HI-3, a compound fractionated from this hot water extract, showed a dose-dependent anti-HSV activity in flow cytometric analysis and plaque reduction assay. 相似文献