共查询到18条相似文献，搜索用时 234 毫秒
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垃圾渗出液对蚕豆根尖细胞的诱变效应 总被引：2，自引：0，他引：2
以不同浓度的垃圾渗出液为诱变剂,测定蚕豆根尖细胞的有丝分裂指数、微核率和染色体畸变率。结果表明:垃圾渗出液能够降低蚕豆根尖细胞有丝分裂指数,能诱发较高频率的微核和染色体畸变,产生染色体断片、滞后染色体和染色体桥。随着垃圾渗出液处理浓度的增加,有丝分裂指数降低,而微核率和染色体畸变率均升高;随着垃圾渗出液浓度的进一步增加,染色体畸变率却下降,但仍明显高于对照组。 相似文献
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用不同浓度（100－500mg/l）的硝酸锌溶液处理蒜小鳞茎,发现随着培养时间的延长和浓度的增加,毒害加重,外观表现为根尖生长受阻,内部表现为细胞有丝分裂指数下降,而分裂细胞异常率增高。分裂细胞异常主要表现为染色体粘连,染色体解体,微核及核解体等,但锌对蒜的毒害作用明显低于镉和铅。 相似文献
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用不同浓度（１００—５００ｍ ｇ／１）的硝酸锌溶液处理蒜小鳞茎,发现随着培养时间的延长和浓度的增加,毒害加重,外观表现为根尖生长受阻,内部表现为细胞有丝分裂指数下降,而分裂细胞异常率增高。分裂细胞异常主要表现为染色体粘连,染色体解体,微核及核解体等,但锌对蒜的毒害作用明显低于镉和铅 相似文献
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张胜 《商丘职业技术学院学报》2005,4(5):59-60
以蚕豆根尖为材料，用微核实验和染色体畸变试验方法，研究不同浓度的8-羟基喹啉与对苯二酚对蚕豆根尖细胞的有丝分裂指数、微核率和染色体畸变率的影响．结果表明：不同浓度的8-羟基喹啉与对苯二酚使蚕豆根尖细胞有丝分裂指数明显增加，诱发较高频率的微核，在一定浓度范围内，其微核率随处理浓度的升高而升高，但随着诱变剂浓度的升高而呈下降趋势．两种诱变剂还能诱导染色体产生多种类型的畸变，染色体畸变率随着处理浓度的增加而升高，随着浓度的进一步增加却呈现下降趋势，但均明显高于对照组． 相似文献
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用不同浓度的亚硒酸钠（se^4 ），分别处理洋葱根尖，结果表明随浓度的增加和染毒时间的延长，有丝分裂指数逐渐下降，且在此过程中还出现微核、染色体滞后、染色体桥等畸变现象。 相似文献
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以蚕豆根尖为材料,研究洗手液对蚕豆根尖细胞的致畸效应,采用蚕豆根尖细胞的微核试验方法和染色体畸变试验方法,以不同浓度的洗手液为诱变剂,测定蚕豆根尖细胞的微核率、有丝分裂指数和染色体畸变率．结果表明：不同浓度的洗手液能诱发较高频率的微核,还能诱导染色体产生多种类型的染色体畸变．结论是洗手液具有一定的遗传毒性． 相似文献
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王春侠 《南阳师范学院学报》2008,7(3):55-58
以蚕豆为实验材料,研究了铽的遗传毒性、细胞毒性.1.蚕豆微核试验、染色体畸变试验结果显示:硝酸铽能诱发蚕豆根尖细胞产生微核以及诱发染色体畸变,且在3～24μg/mL浓度范围内呈剂量-效应关系,说明稀土元素铽对蚕豆根尖具有一定的遗传毒性.2.有丝分裂指数(MI)试验结果显示:低浓度下(3～12μg/mL)的硝酸铽能促进蚕豆根尖细胞有丝分裂,MI升高;但随着浓度的升高,MI下降.说明稀土元素铽对蚕豆根尖具有一定的细胞毒性. 相似文献
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The mutagemc effects of microwave and chromium trioxide （CrO3） on Vicia faba root tip were studied. Micronucleus assay and chromosomal aberration assay were used to determine the mitotic index, the micronucleus frequency and chromosomal aberration frequency of Vicia faba root tip cells induced by microwave and CrO3. The results showed that the micronucleus frequency decreased, and that the mitotic index and chromosomal aberration frequency showed linear dose responses to CrO3, in treatment of microwave for 5 s. In microwave of 25 s, the mitotic index decreased, the micronucleus frequency and chromosomal aberration frequency increased with increase of CrO3 concentration. We concluded that microwave and CrO3 had antagonistic effect on the mitotic index of Vicia faba root tip cells, but had synergetic effect on micronucleus frequency and chromosomal aberration frequency of Viciafaba root tip cells. 相似文献
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钱晓薇 《浙江大学学报(A卷英文版)》2004,5(12)
In this study on the mutagenic effects of different concentrations of chromium trioxide (CrO3) on Vicia faba root tip, micronucleus assay and chromosome aberration assay were used to determine the mitotic indexes, micronucleus rate and chromosome aberration rate of Viciafaba root tip cells. The results showed that the effects of CrO3 concentration on the mitotic indexes were complicated. CrO3 increases the micronucleus rate of Vicia faba root tip cells. It was found that within certain range of CrO3 concentration the micronucleus rate increased systematically with increased concentration of CrO3, but that the micronucleus rate decreased at higher level of CrO3 and that CrO3 also caused various types of chromosome aberration at a rate which increased systematically with increased concentration of CrO3. We concluded that CrO3 has significant mutagenic effect on Viciafaba root tip cells. 相似文献
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以豌豆根尖细胞为材料,用5种品牌的废旧干电池的混合液配制成体积比为5%、10%、15%、20%、25%、30%(V废旧电池液/V蒸馏水)和原液为诱变剂染色体压片技术,测定豌豆根尖细胞的微核率,染色体畸变率和有丝分裂指数等指标.结果表明:不同体积比的废旧电池液均能诱发豌豆根尖细胞较高的微核和染色体畸变,在5%-25%体积比内,微核率随电池液体积比的增加而升高,当体积比大于25%后,微核率逐渐下降;不同体积比的电池液均能降低根尖细胞有丝分裂指数.废旧电池液对豌豆根尖细胞有较强的遗传毒性. 相似文献
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In this study on the mutagenic effects of different concentrations of chromium trioxide (CrO3) on Vicia faba root tip, micronucleus assay and chromosome aberration assay were used to determine the mitotic indexes, micronucleus rate
and chromosome aberration rate of Vicia faba root tip cells. The results showed that the effects of CrO3 concentration on the mitotic indexes were complicated. CrO3 increases the micronucleus rate of Vicia faba root tip cells. It was found that within certain range of CrO3 concentration the micronucleus rate increased systematically with increased concentration of CrO3, but that the micronucleus rate decreased at higher level of CrO3 and that CrO3 also caused various types of chromosome aberration at a rate which increased systematically with increased concentration
of CrO3. We concluded that CrO3 has significant mutagenic effect on Vicia faba root tip cells.
Project supported by Wenzhou Technology Bureau (No. S2002A015) and Wenzhou “551” Talented People Fund of China 相似文献
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Effects of zinc on cadmium uptake by spring wheat （Triticum aestivum, L.）： long-time hydroponic study and short-time ^109Cd tracing study 总被引：2，自引：0，他引：2
To investigate effects of Zn on Cd uptake by spring wheat (Triticum aestivum, L.) in solution culture, long-time hydroponic experiment (1 month) (Experiment 1) and short-time Cd isotope (109Cd) tracing experiment (24 h) (Experiment 2) were conducted. In Experiment 1, spring wheat (cv. Brookton) was grown in nutrient solution at uniform cadmium concentration of 20μ mol/L and 10 zinc concentrations (0, 1, 5, 10, 20, 100, 200, 500, 1000, 2000 μ mol/L). In Experiment 2, spring wheat seedlings,pre-cultivated in complete nutrient solution, were treated with 109Cd of uniform activity and the same series of Zn concentrations as those in Experiment 1 for 24 h. Cd concentrations in shoots and roots in Experiment 1 increased marginally but not consistently with Zn increasing at Zn rates of 1～200 μmol/L, and then decreased significantly at high rates (＞200 μ mol/L). In Experiment 2, the response of 109Cd activities in shoots and roots to increasing Zn was greatly similar to the response of Cd concentrations to Zn increasing in Experiment 1. The results of the two experiments indicated that the short-time and long-time exposure of spring wheat to Zn had similar effects on Cd accumulation. 相似文献
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A hydroponic experiment carried out to study the effect of five Cd levels on growth and photosynthesis of two tomato cultivars showed that the addition of 0.1 μmol/L Cd induced a slight increase in plant height of Hezuo 903 and the SPAD (the Soil-Plant Analyses Development) value of the 2 cultivars. However, at higher Cd levels, i.e., 1 and 10 μmol/L, root length and volume, plant height, and SPAD value were all significantly reduced. On an average of the 2 cultivars, exposure to 1 and 10μmol/L Cd for 33 d reduced plant height by 18.9% and 46.4% and SPAD value by 11.2% and 31.6%, compared with control,respectively. Similarly, root length was reduced by 41.1% and 25.8% and root volume by 45.2% and 63.7%, respectively. The addition of Cd in the growth medium also had significant deleterious effect on net photosynthetic rate (Pn) and intracellular CO2concentration (Ci), with Pn being reduced by 27.2% and 62.1% at 1 μmol/L and 10 μmol/L Cd treatments compared to the control,respectively, while Ci increased correspondingly by 28.4% and 39.3%. 相似文献
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Jia Y Tang SR Ju XH Shu LN Tu SX Feng RW Giusti L 《Journal of Zhejiang University. Science. B》2011,12(4):313-325
This study was conducted to investigate the combined effects of elevated CO2 levels and cadmium (Cd) on the root morphological traits and Cd accumulation in Lolium multiflorum Lam. and Lolium perenne L. exposed to two CO2 levels (360 and 1 000 μl/L) and three Cd levels (0, 4, and 16 mg/L) under hydroponic conditions. The results show that elevated
levels of CO2 increased shoot biomass more, compared to root biomass, but decreased Cd concentrations in all plant tissues. Cd exposure
caused toxicity to both Lolium species, as shown by the restrictions of the root morphological parameters including root length, surface area, volume, and
tip numbers. These parameters were significantly higher under elevated levels of CO2 than under ambient CO2, especially for the number of fine roots. The increases in magnitudes of those parameters triggered by elevated levels of
CO2 under Cd stress were more than those under non-Cd stress, suggesting an ameliorated Cd stress under elevated levels of CO2. The total Cd uptake per pot, calculated on the basis of biomass, was significantly greater under elevated levels of CO2 than under ambient CO2. Ameliorated Cd toxicity, decreased Cd concentration, and altered root morphological traits in both Lolium species under elevated levels of CO2 may have implications in food safety and phytoremediation. 相似文献
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Turmeric has long been used as a spice and food colouring agent in Asia. In the present investigation, the antimutagenic potential of curcumin was evaluated in Allium cepa root meristem cells. So far there is no report on the biological properties of curcumin in plant test systems. The root tip cells were treated with sodium azide at 200 and 300 μg/ml for 3 h and curcumin was given at 5, 10 and 20 μg/ml for 16 h, prior to sodium azide treatment. The tips were squashed after colchicine treatment and the cells were analyzed for chromosome aberration and mitotic index. Curcumin induces chromosomal aberration in Allium cepa root tip cells in an insignificant manner, when compared with untreated control. Sodium azide alone induces chromosomal aberrations significantly with increasing concentrations. The total number of aberrations was significantly reduced in root tip cells pretreated with curcumin. The study reveals that curcumin has antimutagenic potential against sodium azide induced chromosomal aberrations in Allium cepa root meristem cells. In addition, it showed mild cytotoxicity by reducing the percentage of mitotic index in all curcumin treated groups, but the mechanism of action remains unknown. The antimutagenic potential of curcumin is effective at 5 μg/ml in Allium cepa root meristem cells. 相似文献