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1.
不同碳源对红芝液体发酵影响的研究 总被引：1，自引：0，他引：1
采用摇瓶发酵法,研究了不同碳源对红芝液体发酵的菌丝产量、多糖产量的影响.结果表明,所选8种碳源对红芝液体发酵影响不同,其中可溶性淀粉作为碳营养发酵效果最好,菌丝干重达5.2g/L、胞内粗多糖达0.437g/L、胞外粗多糖达1.469g/L,在工业化生产上,可选用可溶性淀粉作红芝液体发酵碳营养. 相似文献
2.
分离纯化获得一个产碱性蛋白酶的枯草芽孢杆菌（Bacillus subtilis）菌株,并研究了4种不同的碳源（葡萄糖,蔗糖,可溶性淀粉和甘露醇）,分别采用3种不同的浓度梯度（0.5%,1%,1.5%）对其产碱性蛋白酶活力的影响。采用不同碳源培养细菌,然后用folin-酚法测定分泌到胞外的碱性蛋白酶活性,发现培养基中以1%甘露醇为碳源培养时所产胞外碱性蛋白酶活性最高。 相似文献
3.
文章旨在探明不同碳源和氮源对菌核侧耳菌丝生长及胞外聚合物动态积累和化学成分组成影响.采用液体发酵培养研究结果表明:菌核侧耳(虎奶菇)菌丝体在以果糖为碳源的培养液中菌丝生长好于葡萄糖为碳源,生物转化率37.18 ～48.53％,菌丝(冻干)产量12.640.41 ～16.500.51g/L;以酵母粉为氮源的菌丝生长好于蛋白胨为氮源,生物转化率28.06 ～33.68％,菌丝产量9.540.79 ～11.450.73g/L.胞外聚合物含量在接种后的6 ～ 14d或6～ 20d均有一个平稳的高含量,在葡萄糖-酵母粉Gpy培养基中胞外聚合物含量(0.12 ～0.16g/L)明显高于以果糖-酵母粉Fpy培养基(0.11 ～0.12g/L).气相色谱法分析表明:菌核侧耳(虎奶菇)胞外聚合物的单糖组成主要为葡萄糖、甘露糖和半乳糖,而且胞外聚合物含量高于单糖组分的总和,是一种杂多糖.本研究确定可以利用果糖-酵母粉培养基进行菌体增量培养,同时利用葡萄-糖酵母粉培养基进行胞外聚合物的增量培养. 相似文献
4.
高产柠檬酸突变菌株以玉米粉为原料的生物反应器扩大生产研究 总被引：2，自引：0，他引：2
Wei HU Jing LIU Ji-hong CHEN Shu-yang WANG Dong LU Qing-hua WU Wen-jian LI 《Journal of Zhejiang University. Science. B》2014,15(11):1006-1010
研究目的：研究高产黑曲霉突变菌株以玉米粉为原料的生物反应器扩大发酵,以期获得适合于工业化生产柠檬酸的发酵工艺。
创新要点：以玉米粉为原料,系统地研究了筛选得到的高产菌株在50L生物反应器中不同糖浓度发酵生产柠檬酸的特性,最终优化出适合于工业化生产柠檬酸的发酵工艺。
研究方法：（1）利用淀粉酶对粉碎后的玉米进行液化,然后过滤,最终得上清液;（2）以50L生物反应器作为发酵设备,对筛选得到的高产柠檬酸菌株进行扩大培养;（3）通过测定不同培养时期中积累的柠檬酸含量和剩余的残总糖,最终优化出高效率生产柠檬酸的发酵工艺。
重要结论：以不同糖浓度的液化玉米粉上清液作为碳源,突变菌株H4002能积累177．7--196．0g,L的柠檬酸,效率能达到2．96～3．27g/（L·h）,尤其当糖浓度为210g／L,H4002菌株表现出最佳的柠檬酸生产水平,如柠檬酸积累187．5g/L,生产效率达3．13g／（L·h）。上述结果说明了突变菌株H4002拥有快速生产柠檬酸的能力。 相似文献
5.
丙酮酸是一种重要的有机酸.为提高发酵法生产丙酮酸的转化率,研究了初始葡萄糖质量浓度、供氧、补糖方式和流加培养中氮的供给对光滑球拟酵母发酵生产丙酮酸的影响,发现葡萄糖质量浓度在116.2g/L左右较为适宜;实行分阶段供氧控制模式可实现高产量、高产率和高葡萄糖消耗速度;采用连续补糖方式可获得较高的丙酮酸产量;用氨水代替KOH控制pH值进行流加培养发酵过程中,细胞表现出很强的丙酮酸合成能力. 相似文献
6.
目的:研究不同有机碳源对灵芝液体发酵灵芝酸生产的影响。方法:研究不同浓度的玉米粉、小麦粉、荞麦粉下灵芝液体发酵菌丝体干重、灵芝酸含量及产量。结果:培养基玉米粉浓度4g/L时,菌丝体干重和灵芝酸产量分别达到最大值,较对照组提高了1.11和1.26倍;培养基小麦粉浓度10g/L时,干重和产量分别比对照组提高了0.73和0.87倍;培养基荞麦粉浓度7g/L时,干重和产量分别比对照组提高了0.97和1.15倍。培养基玉米粉浓度7g/L时,灵芝酸含量达到最大值23.538mg/g。培养基中玉米粉浓度为4、5、7、10g/L时,灵芝酸产量差异不显著。结论:从生产实际考虑,应使用4g/L的玉米粉优化培养基。 相似文献
7.
刘大翠 《四川职业技术学院学报》2005,15(4):106-108
本文对硫酸盐还原菌产亚硫酸盐还原酶的发酵工艺条件进行了初步研究,主要内容包括硫酸盐还原菌发酵条件：碳源、氮源、温度、起始pH、装液量及发酵周期等.实验研究表明,以尿素作为氮源,以可溶性淀粉为碳源,C：N比为1：3,在初始pH7.0的条件下,接种量为5%,装液量为1：3（v/v）,35℃摇床培养42h,酶活力可达到16.2U/mL. 相似文献
8.
测试了平菇、香菇、柳松茸等8种食用菌菌丝对不同处理的葡萄糖和可溶性淀粉的同化利用情况,并测定了不同处理葡萄糖和可溶性淀粉溶液的还原糖含量.结果表明平菇、滑菇、黑木耳、双孢蘑菇和大肥菇在以抽滤除菌的葡萄糖为唯一碳源的培养基上菌丝生长速度最快;香菇和柳松茸在以高温灭菌的可溶性淀粉为唯一碳源的培养基上菌丝生长速度最快;蛹虫草在以间歇灭菌的可溶性淀粉为唯一碳源的培养基上菌丝生长速度最快.葡萄糖经高温灭菌处理后,还原糖含量降低;而可溶性淀粉经高温灭菌后,还原糖含量略微升高. 相似文献
9.
叶友贤 《三明高等专科学校学报》2014,(6):87-92
对选育的抗营养阻遏产酶黄孢原毛平革菌pc R5305菌株的产酶营养基质与环境条件进行了优化。实验结果表明pc R5305在37℃、初始p H4.5条件下,以酒石酸铵（富氮2.2g/L）,葡萄糖（20g/L）,Mn^2＋（0.2g/L）,Tween 80（1g/L）为主要成分的优质培养基中培养24h后添加5.2mmol/L苯甲醇,pc R5305的产酶活性最高。上述条件下,该菌接种后静置培养6 d,Li P活力可达到2013.59U/L,Mn P活力达到353.42U/L,分别提高了23.41%和27.97%。 相似文献
10.
以高效液相色谱法分别测定了6种碳源和8种氮源对莽草酸发酵菌生产莽草酸能力的影响,结果表明:碳源中葡萄糖和果糖对莽草酸产量影响最大,产量分别达到1.43 mg/L和1.50 mg/L;氮源中蛋白胨对莽草酸的贡献最大,达134.58 mg/L.最终确定后续培养基的成分分别为:葡萄糖、蛋白胨、磷酸氢二钠、磷酸氢二钾、氯化钠. 相似文献
11.
INTRODUCTION The difficulties associated with large-scaleproduction of biotherapeutics provide a constantchallenge to the biotechnology industry. FDA hadadded “therapeutic DNA plasmid vectors” to the listof well-characterized biotechnology product (DoHHs,1996), and gene therapy has moved rapidly fromlaboratory scale to clinical trials. It is urgent to de-velop new protocols to obtain high-quality plasmidswith high yields and minimal or no contamination ofRNA and chromosomal D… 相似文献
12.
Yun Wu Yi-ping Xia Jia-ping Zhang Fang Du Lin Zhang Yi-di Ma Hong Zhou 《Journal of Zhejiang University. Science. B》2016,17(11):892-904
Bulblet development is a problem in global lily bulb production and carbohydrate metabolism is a crucial factor. Micropropagation acts as an efficient substitute for faster propagation and can provide a controllable condition to explore bulb growth. The present study was conducted to investigate the effects of humic acid (HA) on bulblet swelling and the carbohydrate metabolic pathway in Lilium Oriental Hybrids ‘Sorbonne’ under in vitro conditions. HA greatly promoted bulblet growth at 0.2, 2.0, and 20.0 mg/L, and pronounced increases in bulblet sucrose, total soluble sugar, and starch content were observed for higher HA concentrations (≥2.0 mg/L) within 45 d after transplanting (DAT). The activities of three major starch synthetic enzymes (including adenosine 5′-diphosphate glucose pyrophosphorylase, granule-bound starch synthase, and soluble starch synthase) were enhanced dramatically after HA application especially low concentration HA (LHA), indicating a quick response of starch metabolism. However, higher doses of HA also caused excessive aboveground biomass accumulation and inhibited root growth. Accordingly, an earlier carbon starvation emerged by observing evident starch degradation. Relative bulblet weight gradually decreased with increased HA doses and thereby broke the balance between the source and sink. A low HA concentration at 0.2 mg/L performed best in both root and bulblet growth. The number of roots and root length peaked at 14.5 and 5.75 cm, respectively. The fresh bulblet weight and diameter reached 468 mg (2.9 times that under the control treatment) and 11.68 mm, respectively. Further, sucrose/starch utilization and conversion were accelerated and carbon famine was delayed as a result with an average relative bulblet weight of 80.09%. To our knowledge, this is the first HA application and mechanism research into starch metabolism in both in vitro and in vivo condition in bulbous crops. 相似文献
13.
番茄灰霉病菌生物学性状研究 总被引：3，自引：0，他引：3
试验表明:番茄灰霉病菌菌丝在5-30℃均能生长,孢子在5-30℃均可萌发,菌丝生长和孢子萌发最适温度为22℃,菌丝生长和孢子萌发喜偏酸的环境,最适pH值分别为5和6,孢子萌发需较高的湿度(RH≥81%),在不同的培养基里菌丝生长以PDA最佳,对碳源的利用以果糖最佳,对氮源的利用以门冬酰胺最佳,葡萄糖对产孢最有利,谷氨酸、葡萄糖、果糖显著促进孢子萌发,淀粉、蔗糖、精氨酸显著抑制孢子葫发,病菌侵染果实最佳温度为16℃,孢子浓度在1×102-1×106个/ml范围内都能侵染番茄果实发病,浓度在1×105个/ml以上病斑发展速度明显加快. 相似文献
14.
目的：对毕赤酵母重组菌GS115-Ch—Glu菌种进行发酵脱毒条件的优化,为大规模发酵重组毕赤酵母获得高表达目的蛋白奠定基础。方法：以毕赤酵母重组菌为实验菌株,分别以葡萄糖、乳糖、玉米淀粉、蛋白胨、甘油、玉米淀粉＋甘油为碳源,研究不同碳源对菌株细胞生长的影响。结果：毕赤酵母重组菌的最适碳源为玉米淀粉,最适浓度为2．5％。 相似文献
15.
16.
不同碳源对蛹虫草菌丝及子实体生长状况的影响 总被引：1，自引：0，他引：1
目的：首次系统研究蛹虫草液体菌种制备及发菌、出草等人工栽培全过程中所必需的适宜碳源。方法：以葡萄糖、果糖、蔗糖、麦芽糖、淀粉等常见碳源为实验组,比较观察菌种质量、菌丝生长状况、发菌及出草情况。结果：各种碳源对蛹虫草菌丝体生长均有促进作用;液体菌种培养基中添加适量葡萄糖为佳;栽培培养基中添加适量蔗糖或葡萄糖为佳。结论：液体菌种培养基及栽培培养基中添加适量小分子碳源具有促进菌丝生长、提高出草品质的作用。 相似文献
17.
Wei Hu Ji-hong Chen Shu-yang Wang Jing Liu Yuan Song Qing-feng Wu Wen-jian Li 《Journal of Zhejiang University. Science. B》2016,17(4):262-270
The objective of this work was to produce citric acid from corn starch using a newly isolated mutant of Aspergillus niger, and to analyze the relationship between changes in the physiological properties of A. niger induced by carbon ion irradiation and citric acid accumulation. Our results showed that the physiological characteristics of conidia in A. niger were closely related to citric acid accumulation and that lower growth rate and viability of conidia may be beneficial to citric acid accumulation. Using corn starch as a raw material, a high-yielding citric acid mutant, named HW2, was obtained. In a 10-L bioreactor, HW2 can accumulate 118.9 g/L citric acid with a residual total sugar concentration of only 14.4 g/L. This represented an 18% increase in citric acid accumulation and a 12.5% decrease in sugar utilization compared with the original strain. 相似文献
18.
Raw corn starch granules were hydrolysized by glucoamylase in a chemostat. The hydrolysis of three different-sized granules shows that smaller granules undergo more hydrolyzation than larger ones. After 78 h, 97% of the granules was hydrolysized with diameter between 0.15 mm and 0.3 mm at 50℃. When corn starch concentration increased from 100 g/L to 250 g/L, the amount of reducing sugar produced was proportional to the initial substrate concentration and no substrate inhibition phenomenon appeared. In order to study the product inhibition exactly, the product from hydrolysis reaction itself was added into the hydrolysis system at the beginning of starch hydrolysis.Product inhibition with different quantities of product added were studied in the initial several hours,during which period enzyme inactivation could be neglected and product inhibition could be studied separately. The experiments indicate that product inhibition happens when the additional quantity exceeds 9.56 g/L. 相似文献
19.
Studies on optimization of nitrogen sources for astaxanthin production by <Emphasis Type="Italic">Phaffia rhodozyma</Emphasis> 总被引：2，自引：0，他引：2
Ni H Chen QH Ruan H Yang YF Li LJ Wu GB Hu Y He GQ 《Journal of Zhejiang University. Science. B》2007,8(5):365-370
Fermentation ofPhaffia rhodozyma is a major method for producing astaxanthin, an important pigment with industrial and pharmaceutical application. To improve astaxanthin productivity, single factor and mixture design experiments were used to investigate the effects of nitrogen source on Phaffia rhodozyma cultivation and astaxanthin production. Results of single factor experiments showed nitrogen source could significantly affect P. rhodozyma cultivation with respect to carbon source utilization, yeast growth and astaxanthin accumulation. Further studies of mixture design experiments using （NH4）2SO4, KNO3 and beef extract as nitrogen sources indicated that the proportion of three nitrogen sources was very important to astaxanthin production. Validation experiments showed that the optimal nitrogen source was composed of 0.28 g/L （NH4）2SO4, 0.49 g/L KNO3 and 1.19 g/L beef extract. The kinetic characteristics of batch cultivation were investigated in a 5-L pH-stat fermentor. The maximum amount of biomass and highest astaxanthin yield in terms of volume and in terms of biomass were 7.71 mg/L and 1.00 mg/g, respectively. 相似文献
20.
丙烯菊酯农药的微生物生物修复作用研究 总被引：3，自引：0，他引：3
通过富集驯化培养,获得了降解丙烯菊酯的混合微生物．研究结果表明,该混合微生物发挥最优降解力的温度是35℃,pH为7．0,培养时间为72h．丙烯菊酯降解速度和混合培养微生物生长速度对外加碳源有较强的依赖性．在培养液中添加1g／L葡萄糖时,72h丙烯菊酯去除率可达76％;而采用丙烯菊酯作为培养菌生长的唯一碳源、氮源和能源时,72h丙烯菊酯去除率为55％． 相似文献