共查询到19条相似文献，搜索用时 234 毫秒
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水稻米香基因的初步定位 总被引：4，自引：0，他引：4
吴娟 《安徽技术师范学院学报》2005,19(3):1-5
香味是水稻重要食味品质性状之一，但稻米香味的遗传甚为复杂。早有报道水稻米香基因位于第八条染色体上且与RFIP标记RG28紧密连锁。本实验通过水稻籼粳亚种之间的杂交，利用SSR(Simple Sequence Repeat)分子标记的方法对水稻米香基因进行初步定位。结果表明：稻米香味性状受一对隐性基因控制，且位于第八条染色体SSR标记RM8264和RM1109之间，其遗传距离大约2．1cM。这一结果将对于米香基因的精细定位提供了重要依据。 相似文献
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方雪 《合肥师范学院学报》2013,31(3):33-36
水稻节间生长发育有着特定的时空模式,有多个基因参与调控。OEUI基因是水稻中控制倒一节伸长发育的一个关键基因,先前已将它定位在第1染色体上。采用集团分离分析法:提取水稻叶片基因组DNA,通过构建DNA池,依靠PCR技术,进行全基因组扫描,寻找到1号染色体短臂上的SSR标记RM3252与Oeui基因连锁。在RM3252附近发展新的标记,把水稻Oeui基因精细定位在RM3148和RSL0152/0153标记之间,相应的物理距离为876kb。 相似文献
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以IR36 × [(桂花黄×红光稻 )F5×国际大粒香稻 ]F4(香籼型 )复合杂交为组合方式 ,研究F2 、F3单粒稻米香味的遗传特点。结果表明 :杂种稻米 (F2 )无香味 :有香味符合 15∶1的遗传分离化。取用F2 的纯合香型单粒种植 ,经剥蘖繁殖得 4 8个F3样品 ,经分组取样分析 ,得F3香味程度分离符合数量遗传规律。结合有关研究提出 :水稻香味性状遗传为质量———数量遗传 ,其遗传控制主要受制于两对独立遗传的隐性主基因 ,同时有至少两对微效基因参与作用。介绍了稻米香味与食味的关系及稻米香味品质质量的评价 相似文献
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SSR和ISSR分子标记已广泛应用于研究遗传行为和基因定位。在对水稻早世代稳定遗传特性的研究中,选用分布于水稻12条染色体上的168对多态性较好的SSR引物,对28个稳定株系的F_1、F_2、F_3及同组合的分离株系进行了SSR标记分析；选用26个多态性较好的ISSR引物对5个组合中出现的8个稳定株系及同组合的分离株系进行了ISSR分子标记。结果显示：稳定株系的标记带型出现三种类型：(1)母本带出现,父本带消失；(2)父本带出现,母本带消失；(3)出现非父母标记带型。分离株系表现为杂合标记带。推测水稻早世代稳定是在遗传因子作用下,在杂交受精或合子早期发育中发生了双亲染色体重排,形成纯合的F_1单株。 相似文献
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很多遗传题需要首先对基因是位于常染色体上还是性染色体上、是连锁在1对同源染色体上还是分别位于不同对的同源染色体上作出正确判断,怎样分析题目信息将基因定位在染色体上呢?本文主要结合近几年的高考试题介绍几种有效的方法。一、用特殊杂交组合判断是否为伴性遗传XBXb×XBY、XbXb×XBY这2个杂交组合,由于后代性状表现与性别相关联,可以用来鉴别基因是位于x染色体上还是位于常染色体上。 相似文献
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Jiang-hua Shi Xi Hao Zhong-chang Wu Ping Wu 《Journal of Zhejiang University. Science. B》2009,10(10):777-783
Root gravitropism is one of the important factors to determine root architecture. To understand the mechanism un-derlying root gravitropism, we isolated a rice (Xiushui63) mutant defective in root gravitropism, designated as glsl. Vertical sections of root caps revealed that glsl mutant displayed normal distribution of amyloplast in the columella cells compared with the wild type. The glsl mutant was less sensitive to 2,4-dichlorophenoxyacetic acid (2,4-D) and a-naphthaleneacetic acid (NAA) than the wild type. Genetic analysis indicated that the phenotype of glsl mutant was caused by a single recessive mutation, which is mapped in a 255-kb region between RM16253 and CAPSI on the short arm of chromosome 4. 相似文献
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Plastids of nongreen tissues import carbon as a source of biosynthetic pathways and energy, and glucose 6-phosphate is the preferred hexose phosphate taken up by nongreen plastids. A cDNA clone encoding glucose 6-phosphate/phosphate translocator (GPT) was isolated from a cDNA library of immature seeds of rice and named as OsGPT. The cDNA has one uninterrupted open reading frame encoding a 42 kDa polypeptide possessing transit peptide consisting of 70 amino acid residues. The OsGPT gene maps on chromosome 8 of rice and is linked to the quantitative trait locus for 1000-grain weight. The expression of OsGPT is mainly restricted to heterotrophic tissues. These results suggest that glucose 6-phosphate imported via GPT can be used for starch biosynthesis in rice nongreen plastids. 相似文献
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INTRODUCTIONNongreenplastidsofheterotrophictissuesarecarbohydrate importingorganellesand ,inthecaseofamyloplastsofstoragetissues,thesiteofstarchsynthesis.Investigationofwholetissuesfromavarietyofstarchsynthesizingcropplantsindicatedthathexoseunitswereimpo… 相似文献
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Insertion mutagenesis has become one of the most popular methods for gene functions analysis. Here we report a two-element Ac/Ds transposon system containing enhancer trap and gene trap for gene tagging in rice. The excision of Ds element was examined by PCR amplification. The excision frequency of Ds element varied from 0% to 40% among 20 F2 populations derived from 11 different Ds parents. Southern blot analysis revealed that more than 70% of excised Ds elements reinserted into rice genome and above 70% of the reinserted Ds elements were located at different positions of the chromosome in rice. The result of histochemical GUS analysis indicated that 28% of enhancer trap and 22% of gene trap tagging plants displayed GUS activity in leaves, roots, flowers or seeds. The GUS positive lines will be useful for identifying gene function in rice. 相似文献
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INTRODUCTIONAfterthewholericegenomewassequencedsuccessfully,moreandmoregenescanbepredictedwithdifferentsoftwaresandannotationsystem.However,thefunctionsofpredictedgeneshavetobefurtheridentifiedbybiologicalanalysis.Oneofthemostpowerfulmethodsassigningfunctiontogeneisthroughinsertionmutagenesiswithtrans-posableelementasDNAsequencetag.Ac/Dstrans-posonsystemhasbecomeaverypopulartoolforgenetaggingandfunctionalgenomicsinvariousplantspecies(Altmannetal.,1995;Chinetal.,1999;Enokietal.,1999;Gre… 相似文献
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Insertion mutagenesis has become one of the most popular methods for gene functions analysis. Here we report a two-elementAc/Ds transposon system containing enhancer trap and gene trap for gene tagging in rice. The excision ofDs element was examined by PCR amplification. The excision frequency ofDs element varied from 0% to 40% among 20 F2 populations derived from 11 differentDs parents. Southern blot analysis revealed that more than 70% of excisedDs elements reinserted into rice genome and above 70% of the reinsertedDs elements were located at different positions of the chromosome in rice. The result of histochemical GUS analysis indicated
that 28% of enhancer trap and 22% of gene trap tagging plants displayed GUS activity in leaves, roots, flowers or seeds. The
GUS positive lines will be useful for identifying gene function in rice.
Project supported by the Hi-Tech Research and Development Program (863) of China (No. 2002AA2Z1003) 相似文献