Microfluidic impedance spectroscopy as a tool for quantitative biology and biotechnology     

Ahmet C. Sabuncu  Jie Zhuang  Juergen F. Kolb  Ali Beskok 《Biomicrofluidics》2012,6(3)

A microfluidic device that is able to perform dielectric spectroscopy is developed. The device consists of a measurement chamber that is 250 μm thick and 750 μm in radius. Around 1000 cells fit inside the chamber assuming average quantities for cell radius and volume fraction. This number is about 1000 folds lower than the capacity of conventional fixtures. A T-cell leukemia cell line Jurkat is tested using the microfluidic device. Measurements of deionized water and salt solutions are utilized to determine parasitic effects and geometric capacitance of the device. Physical models, including Maxwell-Wagner mixture and double shell models, are used to derive quantities for sub-cellular units. Clausius-Mossotti factor of Jurkat cells is extracted from the impedance spectrum. Effects of cellular heterogeneity are discussed and parameterized. Jurkat cells are also tested with a time domain reflectometry system for verification of the microfluidic device. Results indicate good agreement of values obtained with both techniques. The device can be used as a unique cell diagnostic tool to yield information on sub-cellular units.  相似文献   

浅谈力学教学中提升学生的创新能力与素质     

田阿利 《科教文汇》2012,(31):32-33

力学教学对当代大学生的创新能力和素质的培养有着重要的作用.该文结合教学实践和体会,通过力学史的渗透、多种教学手段相结合、“一题多解”的训练、使用力学知识解决生活实际问题和鼓励学生参加课程实践活动等方式,不断激发学生学习兴趣,提高思维积极性,培养创新精神,加强创新能力和科学素质.  相似文献   

Allies in learning: critical insights into the importance of staff–student interactions in university education     

Sarah Richardson  Ali Radloff 《Teaching in Higher Education》2013,18(6):603-615

Discussions of the quality of learning in university education often focus on curricula. Less attention is paid to the role of student–staff interactions. In a context in which a host of factors place pressure on the opportunities for students and staff to interact, it is important to use empirical insights to inform decisions about how to optimise learning. This paper uses data from a large survey of students and teaching staff in Australia to suggest that students and staff should be regarded as allies in learning. It investigates student reports to suggest that frequent interactions with those who teach them lead to higher levels of student engagement and satisfaction and lower attrition rates. The advantages do not only flow in one direction. Teaching staff gain insights into students' learning experiences, providing them with clues to better target their teaching.  相似文献   

Students' perception of university teaching behaviours     

Akhtar Ali  Riaz H. Tariq  J. Topping 《Teaching in Higher Education》2013,18(6):631-647

The purpose of the paper is to explore Students' perception of university teaching behaviours in Pakistan. Three hundred and fifty students from the six public sector Pakistani universities returned questionnaires. Assessment framework, learning activities and instructional strategies emerged from factor analysis as common factors. Students' views on five conceptual variables were also analysed: instructional design, active teaching, learning activities, questioning styles; and assessment framework. University teaching in Pakistan was found to be driven by assessment. This is a matter of concern for all stake holders, including the administration of the universities, teachers, students and the Higher Education Commission. A teaching behaviour paradigm shift is required, switching from knowledge-oriented teaching behaviour focused on successful passage through assessment, to teaching behaviour focused upon the development of transferable skills demanded by external markets.  相似文献   

Rorty's Approach to Cultural Difference: The Conflict between Solidarity and Procedural Liberalism     

Seyed Mohammad Ali Taghavi 《Culture, Theory & Critique》2013,54(2):123-138

Although Richard Rorty's controversial thought has been the subject of many works, there has not been much discussion about his approach to cultural difference. The purpose of this article is to examine how Rorty sees cultural differences philosophically and how he deals with them politically. For him, rationality, morality and even the scope of moral responsibility towards others are defined in terms of solidarity with a particular community and ‘ethnocentrism’, rather than in terms of a universal and metaphysically derived set of criteria. It can be argued that since language, language games and values are mainly components of culture, the latter is one of the most significant communities with which Rorty is concerned, though he is not explicit about it. Nevertheless, Rorty does not think that the moral and philosophical significance of communities, particularly cultural communities, should be reflected in politics. The main argument of the article is that there is an unresolved tension in Rorty's thought between philosophical, moral and emotional attribution of significance to communities and arguably cultural communities, and a disregard for them in the political sphere. This paves the way for outlining an approach to cultural difference that is more just and even more in harmony with Rorty's own philosophy.  相似文献   

Efficiency of antibiotics and gamma irradiation in eliminating Streptomyces strains isolated from paintings of ancient Egyptian tombs     

Mahmoud E.F. Abdel-Haliem  Mona F. Ali  Mohamed F. Ghaly  Akmal A. Sakr 《Journal of Cultural Heritage》2013,14(1):45-50

Forty-six Streptomyces strains were isolated from paintings and stone surfaces from Tell Basta and Tanis tombs (80 km south-east Cairo, Egypt). Eight of these strains were selected to determine their sensitivity against 13 antibiotics. In general, high levels of resistance could be observed. Gentamycin, spiramycin and doxycycline were the most effective antibiotics against the majority of strains under study. Due to the observed antibiotic resistances, gamma irradiation was studied as a possible alternative to inhibit microbial growth. Isolated bacteria were exposed to different doses of gamma irradiation (5, 10, 15, 20 and 25 kGy). The growth of all Streptomyces isolates except S. canarius was completely inhibited at 25 kGy. The applied doses of gamma irradiation did not cause any observable alterations or colour changes to pigments and binding media (arabic gum, animal glue and egg-yolk) used in the paintings.  相似文献   

Neural dynamic sliding mode control of nonlinear systems with both matched and mismatched uncertainties     

Ali Karami-Mollaee  Hamed Tirandaz  Oscar Barambones 《Journal of The Franklin Institute》2019,356(8):4577-4600

Mismatched uncertainty and chattering appear as two challenges in sliding mode control. To overcome the problem of mismatched uncertainty, multiple sliding surfaces with virtual inputs are proposed. Accordingly, we have proposed two new methods based on designed neural observer: sliding mode control (SMC) and dynamic sliding mode control (DSMC) methods. Although, the proposed SMC can significantly cope with the mismatched uncertainties, but it suffers from chattering phenomenon. The chattering problem can be removed in DSMC, because an integrator is placed before the system. This results in increased number of the system states. This new state can be identified with the proposed neural observer. Note that in both proposed approaches, the robust performance (invariance property) of system is reserved, even in the presence of mismatch uncertainties. Then, to have a valid comparison the proposed DSMC is also designed using loop transfer recovery observer (LTRO). This comparison shows the good performance of the DSMC based neural networks. Moreover, the upper bound of uncertainties is not used in SMC and DSMC controllers and also in the neural observer and LTRO, which is important in practical implementation. Finally, comparing the equations, one can see the simplicity of DSMC in concept and also in realization.  相似文献   

Observer based leader-following consensus of second-order multi-agent systems with nonuniform sampled position data     

Syed Ali Ajwad  Tomas Ménard  Emmanuel Moulay  Michael Defoort  Patrick Coirault 《Journal of The Franklin Institute》2019,356(16):10031-10057

This paper deals with the leader-following consensus problem of multi-agent systems with the consideration that each agent can only transmit its position state to the neighbors at irregular discrete sampling times. In the proposed algorithm, a continuous-discrete time observer is designed for the continuous estimation of both position and velocity from the discrete position information of the neighbors. These estimated states are then used for designing a continuous control law which solves the leader-following consensus problem. Moreover, the dynamics of the leader is not fixed and can be controlled through an external input. The stability analysis has been carried out by employing the Lyapunov approach which provides sufficient conditions to tune the parameters according to the maximum allowable sampling period. The developed algorithm has been simulated and then tested on an actual multi-robot system consisting of three differential drive wheeled robots. Both simulation and hardware results validate the effectiveness of the control algorithm.  相似文献   

Profile and Motivations of Registered Whole-Body Donors in Turkey: Istanbul University Experience     

İlke Ali Gürses  Ahmet Ertaş  Başak Gürtekin  Osman Coşkun  Mehmet Üzel  Özcan Gayretli  Mehmet Selman Demirci 《Anatomical sciences education》2019,12(4):370-385

Little is known regarding the profiles of whole body donors in Muslim majority countries where donation is scarce. Therefore, this study aims to profile registered donors in Turkey by means of a survey. The explored data could be used to improve ongoing campaign efforts and ethical practices such as commemoration services. Registered donors of the donation programs at the two faculties of medicine of Istanbul University were compared with the national population and a cluster analysis was performed to reveal any concealed sub-groups. Data from 188 respondents were analyzed. The majority of registered donors were married (42%), male (65.4%), aged over 50 years (76%), held a tertiary education degree (49.7%), and were irreligious (58.5%). Cluster analysis revealed two groups with significantly different educational levels, marital statuses, and religious choices. Regarding whether their bodies could be used for education or research, the majority (64.5%) of the respondents left the decision to the anatomy department. Similarly, 73.8% approved indefinite use of their organs, body parts and/or skeletons. The respondents were also willing to share their medical history (94.2%) and personal information (81.6%) if needed. Motivational themes for body donation including usefulness, impermanence, religion, awareness, and kinship were devised after a thematic analysis. Among the respondents, 56.5% were registered organ donors and 63.3% were frequent blood donors. The results of this study provide data that may help revising informed consent forms, developing and implementing thanksgiving ceremonies, and selecting additional targets for supporting body donation campaign activities such as organ and blood donation units.  相似文献   

Neural embedding-based indices for semantic search     

Fatemeh Lashkari  Ebrahim Bagheri  Ali A. Ghorbani 《Information processing & management》2019,56(3):733-755

Traditional information retrieval techniques that primarily rely on keyword-based linking of the query and document spaces face challenges such as the vocabulary mismatch problem where relevant documents to a given query might not be retrieved simply due to the use of different terminology for describing the same concepts. As such, semantic search techniques aim to address such limitations of keyword-based retrieval models by incorporating semantic information from standard knowledge bases such as Freebase and DBpedia. The literature has already shown that while the sole consideration of semantic information might not lead to improved retrieval performance over keyword-based search, their consideration enables the retrieval of a set of relevant documents that cannot be retrieved by keyword-based methods. As such, building indices that store and provide access to semantic information during the retrieval process is important. While the process for building and querying keyword-based indices is quite well understood, the incorporation of semantic information within search indices is still an open challenge. Existing work have proposed to build one unified index encompassing both textual and semantic information or to build separate yet integrated indices for each information type but they face limitations such as increased query process time. In this paper, we propose to use neural embeddings-based representations of term, semantic entity, semantic type and documents within the same embedding space to facilitate the development of a unified search index that would consist of these four information types. We perform experiments on standard and widely used document collections including Clueweb09-B and Robust04 to evaluate our proposed indexing strategy from both effectiveness and efficiency perspectives. Based on our experiments, we find that when neural embeddings are used to build inverted indices; hence relaxing the requirement to explicitly observe the posting list key in the indexed document: (a) retrieval efficiency will increase compared to a standard inverted index, hence reduces the index size and query processing time, and (b) while retrieval efficiency, which is the main objective of an efficient indexing mechanism improves using our proposed method, retrieval effectiveness also retains competitive performance compared to the baseline in terms of retrieving a reasonable number of relevant documents from the indexed corpus.  相似文献