Examination of the role of transient receptor potential vanilloid type 4 in endothelial responses to shear forces     

Sara Baratchi  Francisco J. Tovar-Lopez  Khashayar Khoshmanesh  Megan S. Grace  William Darby  Juhura Almazi  Arnan Mitchell  Peter McIntyre 《Biomicrofluidics》2014,8(4)

Shear stress is the major mechanical force applied on vascular endothelial cells by blood flow, and is a crucial factor in normal vascular physiology and in the development of some vascular pathologies. The exact mechanisms of cellular mechano-transduction in mammalian cells and tissues have not yet been elucidated, but it is known that mechanically sensitive receptors and ion channels play a crucial role. This paper describes the use of a novel and efficient microfluidic device to study mechanically-sensitive receptors and ion channels in vitro, which has three independent channels from which recordings can be made and has a small surface area such that fewer cells are required than for conventional flow chambers. The contoured channels of the device enabled examination of a range of shear stresses in one field of view, which is not possible with parallel plate flow chambers and other previously used devices, where one level of flow-induced shear stress is produced per fixed flow-rate. We exposed bovine aortic endothelial cells to different levels of shear stress, and measured the resulting change in intracellular calcium levels ([Ca²⁺]_i) using the fluorescent calcium sensitive dye Fluo-4AM. Shear stress caused an elevation of [Ca²⁺]_i that was proportional to the level of shear experienced. The response was temperature dependant such that at lower temperatures more shear stress was required to elicit a given level of calcium signal and the magnitude of influx was reduced. We demonstrated that shear stress-induced elevations in [Ca²⁺]_i are largely due to calcium influx through the transient receptor potential vanilloid type 4 ion channel.  相似文献   

Reactivity effects in video-based classroom research: an investigation using teacher and student questionnaires as well as teacher eye-tracking     

Anna-Katharina Praetorius  Nora A. McIntyre  Robert M. Klassen 《Zeitschrift für Erziehungswissenschaft》2017,20(1):49-74

One prominent problem of conducting observational assessments of teaching quality is the possibility of reactivity effects. To date, the issue of reactivity has received limited empirical attention. The present study, therefore, investigated reactivity in 447 students from 24 classes as well as their 12 teachers. We compared reactivity during lessons that were video-recorded with those that were not: according to t?test analyses of teacher ratings and MIMIC analyses of student ratings, no significant differences emerged in teaching quality or teaching practices. Significant differences were found in teacher and student emotions, as well as in student cognition and behavior. Supplementary eye-tracking analyses indicated reactivity depleted after 1?min 20?s. The results are discussed with respect to their relevance for future video studies on classroom instruction.  相似文献   

Person-Environment Fit and its Effects on University Students: A Response Surface Methodology Study     

Brad Gilbreath  Tae-Yeol Kim  Brooke Nichols 《Research in higher education》2011,52(1):47-62

The amount of time, effort, and money expended in pursuit of a college degree makes it important that students choose a university that is a good fit for them. Unfortunately students often determine whether a university is a fit for them through trial and error. This research investigated student-university fit and its relationship with satisfaction and well-being. We assessed student-university fit by developing 18 fit factors and measuring needs for, and supplies of, those factors. We tested our hypotheses using polynomial regression analysis and response surface methodology. Data from 228 students suggest that student-university fit is predictive of students?? satisfaction with their university and psychological well-being.  相似文献   

Correction to: The State of Current Reading Intervention Research for English Learners in Grades K–2: a Best-Evidence Synthesis     

Roberts  Garrett J.  Hall  Colby  Cho  Eunsoo  Coté  Brooke  Lee  Jihyun  Qi  Bingxin  Van Ooyik  Jacklyn 《Educational Psychology Review》2022,34(1):473-473

Educational Psychology Review -  相似文献   

The State of Current Reading Intervention Research for English Learners in Grades K–2: a Best-Evidence Synthesis     

Roberts  Garrett J.  Hall  Colby  Cho  Eunsoo  Cot&#;  Brooke  Lee  Jihyun  Qi  Bingxin  Van Ooyik  Jacklyn 《Educational Psychology Review》2022,34(1):335-361

Educational Psychology Review - This best-evidence synthesis reviews the past 20 years of rigorous reading intervention research to identify effective programs of instruction for Grade K–3...  相似文献   

Teacher gender: a predictor of special education referral?     

L L McIntyre 《Journal of learning disabilities》1988,21(6):382-383

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Can ‘pooling teaching tips’ be more than ‘pooling teaching tips’?     

Colin O’Byrne  Gwynaeth McIntyre  Celia Lie  Sheena Townsend  Benjamin Schonthal 《Tertiary Education and Management》2013,19(4):351-361

Abstract

There is increasing interest in how academic development of various kinds influences university teaching and student learning. To date the focus has been on formal, expert-led opportunities to learn how to teach. Our institution has developed a less formal, participant-led forum for teaching staff that was initially established to share ideas on teaching techniques and skills. We report here on participant-led research that explores if and how this model of group learning works, and how it might relate to other models that have been applied to tertiary teaching development. Authors adopted a self-study research framework incorporating a collaborative autoethnography. The data emphasises how participants use this forum as a community of practice, as a means for deep engagement with learning about teaching, and as a means to rationally manage their learning against a backdrop of challenges associated with learning to teach in research-led higher education.  相似文献   

‘He's just enthusiastic. Is that such a bad thing?’ Experiences of parents of children with Attention Deficit Hyperactivity Disorder     

Ruth McIntyre  Eilis Hennessy 《Emotional and Behavioural Difficulties》2013,18(1):65-82

Parenting a child with Attention Deficit Hyperactivity Disorder (ADHD) is a challenging experience. The hyperactivity, impulsivity and inattention of a child with ADHD often put parenting skills to the test. The present study thus aimed to explore the experiences of parents of children with ADHD in Ireland. Eighteen parents of 7–12-year-old boys with a diagnosis of ADHD took part in open-ended interviews. Thematic analysis was carried out on the interview content. Six major themes were identified: (1) getting your head around ADHD; (2) the child takes over; (3) emotional impact; (4) inconsistency of structural supports; (5) ignorance and discrimination; and (6) it's not all bad. Results are discussed in terms of the need to implement family-centred supports for ADHD. The importance of educating the population at large about ADHD is also discussed. Finally, the need to take a more positive, strengths-based approach to ADHD is highlighted.  相似文献   

Why they sat still: the ideas and values of long‐serving teachers in challenging inner‐city schools in England     

Joanna McIntyre 《Teachers and Teaching》2013,19(5):595-614

Within the UK there are grave concerns about retention and attrition rates within the teaching profession, particularly in challenging schools. These are compounded by worries about the gap that will be left as long‐serving teachers reach retirement age. This article is about the working lives of long‐serving teachers in three high‐poverty urban schools in England. In a climate in which teaching is tightly controlled and suffering from problems of retention and recruitment, the teachers discuss intensely personal and emotional commitments to their work‐place. Qualitative in‐depth interviews with 20 long‐serving teachers, all of whom had management responsibilities, are used to explore their lives and careers. These histories evoke a strong sense of the ideas and values that make up their personal and professional identities. These are then contrasted with the ideas and values in officially mandated views of progression within the profession. Within the stories of their professional lives, the teachers talk about the emotional dimensions of their work and the emotional ties of their ‘work‐place’. The article concludes that recognition of the emotional dimensions within teachers’ work at an official level could go some way to helping with recruitment and retention in schools facing challenging circumstances.  相似文献   

‘Just turn to us’: the persuasive features of contract cheating websites     

Susan Rowland  Christine Slade  Kai-Sheng Wong  Brooke Whiting 《Assessment & Evaluation in Higher Education》2018,43(4):652-665

Academic integrity is important to universities and students must abide by codes of academic conduct around assessment. Students are, however, subject to multiple pressures around assessment, some of which can push them to cheat. Modern contract cheating websites are the fronts for sophisticated, commercial operations that offer individually written assessment items for a fee; to combat their use we need a better understanding of the tools they use to persuade students to become customers. In this study we examined the persuasive features of 11 highly-visible contract cheating websites and mapped these features to a previously used persuasiveness framework. We find that contract-cheating websites use a variety of credibility, interactive and informative features designed to persuade students to use their services. In addition, the sites offer low-cost, customisable products available in very short timeframes. We suggest ways in which educators can encourage academic integrity by talking with their students about how the websites promulgate their ‘just turn to us’ message.  相似文献