Smartphone-interfaced lab-on-a-chip devices for field-deployable enzyme-linked immunosorbent assay     

Arnold Chen  Royal Wang  Candace R. S. Bever  Siyuan Xing  Bruce D. Hammock  Tingrui Pan 《Biomicrofluidics》2014,8(6)

The emerging technologies on mobile-based diagnosis and bioanalytical detection have enabled powerful laboratory assays such as enzyme-linked immunosorbent assay (ELISA) to be conducted in field-use lab-on-a-chip devices. In this paper, we present a low-cost universal serial bus (USB)-interfaced mobile platform to perform microfluidic ELISA operations in detecting the presence and concentrations of BDE-47 (2,2′,4,4′-tetrabromodiphenyl ether), an environmental contaminant found in our food supply with adverse health impact. Our point-of-care diagnostic device utilizes flexible interdigitated carbon black electrodes to convert electric current into a microfluidic pump via gas bubble expansion during electrolytic reaction. The micropump receives power from a mobile phone and transports BDE-47 analytes through the microfluidic device conducting competitive ELISA. Using variable domain of heavy chain antibodies (commonly referred to as single domain antibodies or Nanobodies), the proposed device is sensitive for a BDE-47 concentration range of 10⁻³–10⁴μg/l, with a comparable performance to that uses a standard competitive ELISA protocol. It is anticipated that the potential impact in mobile detection of health and environmental contaminants will prove beneficial to our community and low-resource environments.  相似文献   

Book reviews     

J. Bruce Francis  Michalina Vaughan  Colin Flood Page  Sally Tomlinson  R. A. Becher  Margaret C. Ives  John Urry  K. G. Collier  Meir Zadok  Charles Carter  Jürgen Schramm  Jean Barry  Graeme C. Moodie  Donald Bligh  R. A. King  D. G. Mulcahy  Peter Scott  F. R. Charmer  T. R. Bone  John A. Hughes  Roger Greenhough  Alec M. Ross  John Wakeford  Joyce Skinner  Duncan B. Nimmo  Cedric Cullingford  L. R. B. Elton 《Higher Education》1979,8(6):709-744

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<Emphasis Type="Bold">Battling Our Decline</Emphasis>     

Bruce S. Thornton 《Academic Questions》2017,30(4):514-518

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The Strange Birth of Santa Claus: From Artemis the Goddess and Nicholas the Saint     

Bruce Curtis 《Journal of American culture (Malden, Mass. : 2003)》1995,18(4):17-32

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Teacher Education and the erasmus Programme∗     

Mike Bruce 《欧洲师范教育杂志》1989,12(3):197-228

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Introducing Our Invited Essay Series; “Critical Engagements with the Politics of ‘Extremism’ and Schooling”     

Bruce Collet 《Diaspora, Indigenous, and Minority Education》2017,11(1)

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创业者的白金思维法     

Bruce D.Henderson 《科技创业月刊》2002,(4):33

任何一个复杂的问题都是近乎无数的事实和关系的组合。商业行为尤其受到方方面面的影响,包括过去的、非逻辑的和不可知的事物的影响。这种复杂性又因目标不同与服务于各种各样的顾客的需要而更加盘根错节,而顾客的许多目标不得不经过权衡后放弃。在这样复杂的条件下解决问题需要井井有条的、系统的方法以试图优化最后的决定。  相似文献   

Emotion discourse, social cognition, and social skills in children with and without developmental delays     

Fenning RM  Baker BL  Juvonen J 《Child development》2011,82(2):717-731

This study examined parent-child emotion discourse, children's independent social information processing, and social skills outcomes in 146 families of 8-year-olds with and without developmental delays. Children's emergent social-cognitive understanding (internal state understanding, perspective taking, and causal reasoning and problem solving) was coded in the context of parent-child conversations about emotion, and children were interviewed separately to assess social problem solving. Mothers, fathers, and teachers reported on children's social skills. The proposed strengths-based model partially accounted for social skills differences between typically developing children and children with delays. A multigroup analysis of the model linking emotion discourse to social skills through children's prosocial problem solving suggested that processes operated similarly for the two groups. Implications for ecologically focused prevention and intervention are discussed.  相似文献   

How do students define their roles and responsibilities in online learning group projects?     

Karen C. Williams  Kari Morgan  Bruce A. Cameron 《Distance Education》2011,32(1):49-62

The goal of this study was to explore the processes of group role formation in online class settings. Qualitative analysis was used to code chat logs and discussion threads in six undergraduate Family and Consumer Sciences online courses that required online group projects. Four themes related to the process of group role formation emerged: testing the waters, apologies as being nice, tag – you’re it, and struggling to find one’s role. Students created roles of leader, wannabe, spoiler, agreeable enabler, coat‐tails, and supportive worker as the group process evolved over the course of the semester. Results lend support for a balance between allowing students to create and experience roles on their own and faculty assignment of roles. Questions are raised related to faculty approaches toward directing and scaffolding the group process.  相似文献   

Use of dual-task methodology for skill assessment and development: examples from rugby league   总被引：2，自引：1，他引：1  

Gabbett T  Wake M  Abernethy B 《Journal of sports sciences》2011,29(1):7-18

We assessed the attentional demands of drawing and passing in rugby league players and investigated the effects of single-task and dual-task training on the acquisition, retention, and transfer of skill in these athletes. In Study 1, high-skilled and lesser-skilled rugby league players performed a standardized 2-on-1 drill under single-task (primary skill in isolation) and dual-task (primary skill while performing a secondary verbal tone recognition task) conditions. No differences were detected in primary task performance between groups, although the performance of the high-skilled players was more resistant to skill decrement under dual-task conditions. In Study 2, high-performance rugby league players were randomly allocated to either a single-task or dual-task training group. Each group underwent 8 weeks of training between the pre- and post-test sessions. While the mean improvement for draw and pass proficiency under dual-task conditions in the dual-task training group was greater than in the single-task training group (10.0% vs. 2.3%), the differences, while providing a moderate effect size (d = 0.57), were not statistically significant. These results suggest that the attentional demands of drawing and passing are reduced in high-skilled rugby league players compared with their lesser-skilled counterparts. In addition, compared with single-task training, dual-task training appears to improve the ability to perform dual-task draw and pass tasks (possibly through an improvement in time-sharing skills). Further studies are required to verify the efficacy of dual-task training as a training stimulus.  相似文献