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排序方式: 共有238条查询结果,搜索用时 62 毫秒
51.
Ming-Cheng Lin Jiraporn Nawarak Tai-Yuan Chen Hsien-Yu Tsai Jung-Feng Hsieh Supachok Sinchaikul Shui-Tein Chen 《Biomicrofluidics》2009,3(3)
Rapid detection of brain natriuretic peptide (BNP) concentration can be used for the diagnosis of acute heart failure and for the evaluation of the effectiveness of a clinical therapy. We used the systematic evolution of ligands by exponential enrichment method to develop DNA aptamers for BNP whose sequences were determined by cloning method and consensus sequence analysis. A total of eight conserved sequences was identified. By combining the fluorescent-labeled aptamers with fast protein lab-on-chip analysis, we could achieve quantification of BNP concentrations with high speed, sensitivity, and specificity. 相似文献
52.
Jason S.-H. Tsai Chao-Lung Wei Shu-Mei Guo Leang S. Shieh Ce R. Liu 《Journal of The Franklin Institute》2008,345(5):508-535
An evolutionary programming-based adaptive observer is presented in this paper to improve the performance of state estimation of nonlinear time-varying sampled-data systems. Also, this paper presents a novel state-space adaptive tracker together with the proposed observer and estimation schemes for nonlinear time-varying sampled-data systems having actuator failures. For the class of slowly varying nonlinear time-varying systems, the proposed methodology is able to achieve the desired fault detection and performance recovery for the originally well-designed systems, as long as the controller having the high-gain property. For practical implementation, we utilize the advantages of digital redesign methodology to convert a well-designed high-gain analog controller/observer into its corresponding low-gain digital controller/observer. Illustrative examples are given to demonstrate the effectiveness of the proposed method. The developed digitally redesigned adaptive tracker with the proposed observer and estimator is suitable for implementation by using microprocessors. 相似文献
53.
Chia-Wen Chen Wen-Chung Wang Ming Ming Chiu Sage Ro 《Journal of Educational Measurement》2020,57(2):343-369
The use of computerized adaptive testing algorithms for ranking items (e.g., college preferences, career choices) involves two major challenges: unacceptably high computation times (selecting from a large item pool with many dimensions) and biased results (enhanced preferences or intensified examinee responses because of repeated statements across items). To address these issues, we introduce subpool partition strategies for item selection and within-person statement exposure control procedures. Simulations showed that the multinomial method reduces computation time while maintaining measurement precision. Both the freeze and revised Sympson-Hetter online (RSHO) methods controlled the statement exposure rate; RSHO sacrificed some measurement precision but increased pool use. Furthermore, preventing a statement's repetition on consecutive items neither hindered the effectiveness of the freeze or RSHO method nor reduced measurement precision. 相似文献
54.
This study aims to explore Taiwanese university students’ conceptions of learning biology as memorizing or as understanding, and their self-efficacy. To this end, two questionnaires were utilized to survey 293 Taiwanese university students with biology-related majors. A questionnaire for measuring students’ conceptions of memorizing and understanding was validated through an exploratory factor analysis of participants’ responses. As for the questionnaire regarding the students’ biology learning self-efficacy (BLSE), an exploratory factor analysis revealed a total of four factors including higher-order cognitive skills (BLSE-HC), everyday application (BLSE-EA), science communication (BLSE-SC), and practical works (BLSE-PW). The results of the cluster analysis according to the participants’ conceptions of learning biology indicated that students in the two major clusters either viewed learning biology as understanding or possessed mixed-conceptions of memorizing and understanding. The students in the third cluster mainly focused on memorizing in their learning while the students in the fourth cluster showed less agreement with both conceptions of memorizing and understanding. This study further revealed that the conception of learning as understanding was positively associated with the BLSE of university students with biology-related majors. However, the conception of learning as memorizing may foster students’ BLSE only when such a notion co-exists with the conception of learning with understanding. 相似文献
55.
Yuping Tsai 《Economics of Education Review》2010,29(4):606-617
Studies examining the wage effect of overeducation have generated very consistent results. Their findings suggest that, for workers with similar educational attainment, workers who are overeducated for the job suffer from significant wage penalties. However, most studies use cross-sectional data, implicitly assuming that workers are randomly assigned to being overeducated. Using data from the Panel Study of Income Dynamics for the period 1979–2005, this study conducts a panel analysis to account for time-constant individual characteristics. It uses a numerical approach to provide the wage effects in the presence of non-classical measurement error in the educational mismatch variables. The results provide evidence that overeducated status does not cause lower earnings. Instead, the significant wage differential found in previous studies is simply a result of ignoring the non-random assignment of workers to jobs. 相似文献
56.
Neural Oscillation Reveals Deficits in Visuospatial Working Memory in Children With Developmental Coordination Disorder 下载免费PDF全文
The electroencephalographic (EEG) oscillations associated with visuospatial working memory (VSWM) were examined in children with developmental coordination disorder (DCD; 10–11 years; N = 29) and typically developing (TD) children (10–11 years; N = 29). Behaviorally, DCD showed poorer VSWM than TD, which coincided with the diminished ability of DCD in modulating neural oscillations. Furthermore, prestimulus oscillatory alpha activity was correlated with VSWM performance. The results suggest that children with DCD might have a reduced ability to encode and recognize new information, and in particular have difficulty in maintaining task‐relevant information, resulting in poorer VSWM. This study thus concludes that changes in oscillatory EEG activity reflect some of the problems leading to cognitive deficits in DCD. 相似文献
57.
Jon-Chao Hong Ming-Yueh Hwang Chow-Chin Lu Chi-Ruei Tsai 《Research in Science Education》2017,47(1):185-201
Microteaching has gained considerable attention for its effectiveness in rapid and contextual training in professional development programs. However, the interpretive quality of the teaching demonstration and peer feedback may influence individuals’ attribution and self-correction, leading to ineffective learning. In this study, a microteaching workshop in a professional development program for 78 elementary school science teachers was investigated. The results showed that the effectiveness of microteaching was negatively affected by participants’ perception bias due to social categorization. Moreover, it was indicated that the participants’ perception of the in-group and out-group, classified by the degree of the individuals’ science knowledge, fostered social categorization. Participants tended to experience perception conflicts caused by their inability to see personal faults, and a typical perception bias of “seeing one’s own strengths and seeing others’ shortcomings” was more frequently recognized in the out-group. These results converge to highlight the importance of social categorization in perception bias relevant to microteaching. 相似文献
58.
We report a new design of microfluidic chip (Multiple electric Field with Uniform Flow chip, MFUF chip) to create multiple electric field strengths (EFSs) while providing a uniform flow field simultaneously. MFUF chip was fabricated from poly-methyl methacrylates (PMMA) substrates by using CO2 laser micromachining. A microfluidic network with interconnecting segments was utilized to de-couple the flow field and the electric field (EF). Using our special design, different EFSs were obtained in channel segments that had an identical cross-section and therefore a uniform flow field. Four electric fields with EFS ratio of 7.9:2.8:1:0 were obtained with flow velocity variation of only 7.8% CV (coefficient of variation). Possible biological effect of shear force can therefore be avoided. Cell behavior under three EFSs and the control condition, where there is no EF, was observed in a single experiment. We validated MFUF chip performance using lung adenocarcinoma cell lines and then used the chip to study the electrotaxis of HSC-3, an oral squamous cell carcinoma cell line. The MFUF chip has high throughput capability for studying the EF-induced cell behavior under various EFSs, including the control condition (EFS = 0). 相似文献
59.
台湾准优秀乒乓球运动员的运动竞赛焦虑、特质性心理坚韧性和运动心理技能间的关系 总被引:2,自引:0,他引:2
Ling-Chun Chen Chia-Wen Hsien Chih-Man Chang Jen-Sen Wang Tsung-Min Hung 《体育科研》2005,26(3):63-66,72
运动员的心理技能在他们获取成功的过程中起着关键作用.目前已归纳出获得优异运动成绩所必需的心理品质.然而,结合乒乓球运动,对这些心理品质间的相互关系的研究却很少.因此,本文主要研究了对乒乓球运动员取得优异成绩起重要作用的几个心理品质间的相互关系.研究对象75名高中生和大学生乒乓球运动员,其中男运动员55名,女运动员20名.研究方法要求所有被试者填写运动竞赛量表(SCAT-A)、特质性心理坚韧性问卷(TMTIS)、运动心理技能问卷(APSI).对所测数据进行皮尔逊相关分析.研究结果TMTIS总量表分与APSI总量表分呈高度相关(r=0.866),而SCAT总分与它们不相关.除了注意力分量表外,APSI量表中的各分量表分均与TMTIS各分量表分呈正相关.APSI量表中的注意力与SCAT总分呈负相关. 相似文献
60.
Shau-Chun Wang Hsien-Hung Wei Hsiao-Ping Chen Min-Hsuan Tsai Chun-Ching Yu Hsueh-Chia Chang 《Biomicrofluidics》2008,2(1)
A transient 106-fold concentration of double-layer counterions by a high-intensity electric field is demonstrated at the exit pole of a millimeter-sized conducting nanoporous granule that permits ion permeation. The phenomenon is attributed to a unique counterion screening dynamics that transforms half of the surface field into a converging one toward the ejecting pole. The resulting surface conduction flux then funnels a large upstream electro-osmotic convective counterion flux into the injecting hemisphere toward the zero-dimensional gate of the ejecting hemisphere to produce the superconcentration. As the concentrated counterion is ejected into the electroneutral bulk electrolyte, it attracts co-ions and produce a corresponding concentration of the co-ions. This mechanism is also shown to trap and concentrate co-ion microcolloids of micron sizes too (macroions) and hence has potential application in bead-based molecular assays. 相似文献