The nucleotide sequence of Shigella flexneri 1A: A common Indian isolate     

Suvidya H. Ranade  Ashraf Hossani  D. N. Deobagkar  D. D. Deobagkar  B. A. Chopade  Pramod S. Khandekar 《Indian journal of clinical biochemistry : IJCB》2009,24(2):142-149

We report complete sequences of 2 genes of S.flexneri 1a an Indian isolate for the first time. Shigella is causative agent of shigellosis or bacillary dysentery. Genomic library was constructed by shotgun approach. Sequencing was carried out using Big Dye terminator chemistry using ABI 3730 48 capillary DNA analyzer. 170 recombinants were subjected to nucleotide sequencing. Sequence data was analyzed, of these 2 clones showed presence of complete genes out of the total clones sequenced. Annotations were done using various bioinformatics tools. Gene Sfo676 on contig SF21B11, 513 bp long codes for a protein 170 aa long with molecular weight of 18836.5 daltons. The protein is 99 % identical to S. flexneri 2a 301 and not with any other strain of Shigella. It has 7 different sites for phosphorylation, myristoylation and glycosylation. Predicted cellular localization is cytoplasmic membrane. SF0368 is another full-length gene SF0368 on contig SF69C1 is a 312 nucleotide long. It is 103 aa long with molecular weight 11394.0 daltons. Protein is 100% identical to S. flexneri 2a 301 and 99% with S. sonnei strain 046. The gene shows difference when compared with S.sonnei in mono and dinucleotide frequency as well as amino acid composition.  相似文献   

浙江省高校学报现状、问题及发展对策   总被引：2，自引：0，他引：2  

钱觉寿  王薇 《科技通报》2009,25(2)

高校学报是我国期刊的重要组成部分,其中的自然科学类学报是我国科技创新和建设创新型国家的主要媒体之一.本文对浙江省50家高校学报的办刊情况等方面进行了调查,分析得出高校学报由于受传统办刊观念的影响,随着我国高等教育改革的不断深化,同样面临着巨大的挑战和机遇.针对目前高校学报普遍存在着办刊思想保守、脱离市场、缺少经营意识等问题,并由此提出了进一步提高浙江省高校学报办刊质量的对策和建议.  相似文献   

Micromixer based on viscoelastic flow instability at low Reynolds number   总被引：1，自引：0，他引：1  

Y. C. Lam  H. Y. Gan  N. T. Nguyen    H. Lie 《Biomicrofluidics》2009,3(1)

We exploited the viscoelasticity of biocompatible dilute polymeric solutions, namely, dilute poly(ethylene oxide) solutions, to significantly enhance mixing in microfluidic devices at a very small Reynolds number, i.e., Re≈0.023, but large Peclet and elasticity numbers. With an abrupt contraction microgeometry (8:1 contraction ratio), two different dilute poly(ethylene oxide) solutions were successfully mixed with a short flow length at a relatively fast mixing time of <10 μs. Microparticle image velocimetry was employed in our investigations to characterize the flow fields. The increase in velocity fluctuation with an increase in flow rate and Deborah number indicates the increase in viscoelastic flow instability. Mixing efficiency was characterized by fluorescent concentration measurements. Our results showed that enhanced mixing can be achieved through viscoelastic flow instability under situations where molecular-diffusion and inertia effects are negligible. This approach bypasses the laminar flow limitation, usually associated with a low Reynolds number, which is not conducive to mixing.  相似文献   

Single nanopore transport of synthetic and biological polyelectrolytes in three-dimensional hybrid microfluidic�Mnanofluidic devices     

Travis L. King  Enid N. Gatimu    Paul W. Bohn 《Biomicrofluidics》2009,3(1)

  相似文献   

Effect of vitamin E supplementation on oxidative stress in hemodialysis patients     

R. B. Bhogade  A. N. Suryakar  N. G. Joshi  R. Y. Patil 《Indian journal of clinical biochemistry : IJCB》2008,23(3):233-237

Hemodialysis represents a chronic stress status for its recipients. Many hypotheses state that this may be associated with oxidative stress. Thus, there may be deficiency of antioxidants like erythrocytic superoxide dismutase, catalase, vitamin E or increased generation of free radicals like superoxide anions. A study was carried out to investigate oxidant and antioxidant status in chronic renal failure patients undergoing hemodialysis and effect of vitamin E supplementation on these two status. Blood samples were collected from patients before and after hemodialysis and from controls. The samples were analyzed for quantitation of MDA as index of lipid peroxide, nitric oxide, vitamin E, vitamin C and enzymatic antioxidants namely erythrocyte SOD and catalase. As compared to controls, the levels of serum MDA were significantly increased and activities of erythrocyte SOD and catalase, levels of serum nitric oxide, serum vitamin E and plasma vitamin C were significantly decreased both before and after hemodialysis. The efficiency of vitamin E therapy in hemodialysis patients was assessed by re-evaluating oxidant and antioxidant status of same patients after supplementation of vitamin E. Vitamin E supplementation caused decrease in serum MDA and increase in levels of serum nitric oxide, vitamin E, vitamin C and activities of erythrocytic SOD and catalase. Our results suggest the presence of oxidative stress and the possible preventive role of vitamin E therapy in hemodialysis patients.  相似文献   

A comparative study on oxidative stress and antioxidant status in ischemic stroke patients with and without diabetes     

Benedicta D’Souza  Vivian D’Souza  S. Sowmya  G. Seema  C. N. Hemalatha  M. Anu  G. Sajana 《Indian journal of clinical biochemistry : IJCB》2008,23(3):218-222

The study was conducted to find out the extent of lipid peroxidation and antioxidant status in ischemic stroke patients (ISPs) with and without diabetes. Malondialdehyde (MDA) was studied as a marker of lipid peroxidation. Glutathione (GSH), uric acid and ceruloplasmin were estimated to study the antioxidant potential of ISPs. Significantly higher levels of MDA were found in both the groups of ISPs and the increase in MDA was more in ISPs without diabetes. GSH levels were decreased significantly in both the groups of ISPs and maximum decline was found in ISPs with diabetes. Uric acid levels were significantly increased in both the groups of ISPs. Ceruloplasmin levels were increased significantly in ISPs without diabetes, whereas its levels were slightly decreased in ISPs with diabetes. A negative correlation was found between MDA and the antioxidants GSH, uric acid and ceruloplasmin in ISPs with diabetes. This study suggests that there is an association between ischemic stroke and increased oxidative stress and the antioxidant potential is impaired in both the groups of ISPs with and without diabetes.  相似文献   

Effect of Vitamin B<Subscript>12</Subscript> and Folate on Homocysteine levels in colorectal cancer     

Sunil Chandy  M. N. Sadananda Adiga  Girija Ramaswamy  C. Ramachandra  Lakshmi Krishnamoorthy 《Indian journal of clinical biochemistry : IJCB》2008,23(3):258-261

Folate and cobalamin (Vitamin B₁₂) are two essential micronutrients involved in one-carbon metabolism, which affects heart disease, neural tube defects and cancer. Methylenetetrahydrofolate reductase, the key enzyme involved in one carbon metabolism produces methyl tetrahydrofolate from methylene tetrahydrofolate, which in turn donates methyl group to homocysteine to generate methionine. There exist two common low function polymorphic variants of the methylenetetrahydrofolate reductase gene involving nucleotides 677 C→T and 1298 A→C, which are associated with hyperhomocysteinemia. These polymorphisms are also linked with increased risk for certain cancers such as breast cancer and at the same time providing a protective effect on colorectal cancer. In this case control study, we have evaluated levels of folic acid, vitamin B₁₂ and homocysteine in patients with colorectal cancer. Folate and homocysteine levels did not differ significantly between the two groups; however an increasing trend was noticed with increase in homocysteine levels. Vitamin B₁₂ levels were increased in cases compared to control group.  相似文献   

Lead toxicity as a result of herbal medication     

A. Ravi Raja  G. N. Vishal Babu  Geraldine Menezes  T. Venkatesh 《Indian journal of clinical biochemistry : IJCB》2008,23(2):200-203

Awareness about the toxic effects of non-essential metals is still lacking in developing countries. Lead is one among them, which ranks second in the Agency for Toxic Substances and Disease Registry’ s top 20 lists of toxic metals. Some of the herbal medicines prepared from certain roots and leaves are known to contain this toxic metal at alarming levels. We have a case of a person who suffered from the toxic effects of lead such as vomiting and colicky abdominal pain after consuming a herbal remedy for Jaundice treatment. This went unrecognized initially because of the presence of multiple problems like Malaria and Renal calculi. Lead poisoning as causative factor for anemia, vomiting and colic were confirmed only when blood lead concentration was estimated. A combination of chelation therapy and nutritional supplementation was found to be useful in reducing the body lead burden.  相似文献   

Association of hyperhomocysteinemia to alcohol withdrawal in chronic alcoholics     

K. Devika Rani  N. Suneetha  Shruti Mohanty  Pragna Rao 《Indian journal of clinical biochemistry : IJCB》2008,23(2):150-153

This study is conducted in chronic alcoholics to assess the association of an Individual’s level of serum homocysteine with the success of achieving alcohol deaddiction in the patient. The patients’ nutrition status is also assessed. 50 chronic alcoholics admitted to a deaddiction center were inducted into the study. Patients underwent an 8 weeks holistic program to promote rehabilitation from alcoholism. All the patients were addicted to alcohol for 8 – 10 years. Of the 50 patients enrolled, 39 of them completed the 8 weeks program including complete abstinence from alcohol during this period. Fasting blood samples were collected on admission and again after 8 weeks of alcohol abstinence for analyses of serum homocysteine and serum prealbumin, transferrin, total proteins and albumin, gammaglutamyl transferase (GGT) and alanine transaminase (ALT). Of the 50 patients enrolled in the study, 39 completed the 8 weeks rehabilitation program. 11 patients discontinued within 2 weeks of admission. During the 8 weeks of complete alcohol abstinence, patients were given a balanced diet and multivitamin supplements. A significant improvement in their nutritional status was noted by the elevation of serum levels of prealbumin, transferrin, total proteins and albumin. Serum homocysteine levels decreased significantly (p<0.002) to normal levels from previous hyperhomocytenemia. This was accompanied by decrease in serum GGT and ALT levels indicating improved liver functions. Serum estimation of homocysteine in chronic alcoholics is important to assess whether the patient will have a successful rehabilitation. Normal homocysteine levels are achieved after dietary changes and abstinence from alcohol. Timely correction of hyperhomocysteinemia also provides successful rehabilitation.  相似文献   

The paradoxical profession: Teaching at the turn of the century   总被引：1，自引：0，他引：1  

Andy Hargreaves  Leslie N. K. Lo 《Prospects》2000,30(2):167-180

  相似文献