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Edwards TL Harper JC Polsky R Lopez DM Wheeler DR Allen AC Brozik SM 《Biomicrofluidics》2011,5(4):44115-4411514
Herein is described the fabrication and use of a plastic multilayer 3-channel microfluidic fixture. Multilayer devices were produced by laser machining of plastic polymethylmethacrylate and polyethyleneterapthalate laminates by ablation. The fixture consisted of an array of nine individually addressable gold or gold/ITO working electrodes, and a resistive platinum heating element. Laser machining of both the fluidic pathways in the plastic laminates, and the stencil masks used for thermal evaporation to form electrode regions on the plastic laminates, enabled rapid and inexpensive implementation of design changes. Electrochemiluminescence reactions in the fixture were achieved and monitored through ITO electrodes. Electroaddressable aryl diazonium chemistry was employed to selectively pattern gold electrodes for electrochemical multianalyte DNA detection from double stranded DNA (dsDNA) samples. Electrochemical detection of dsDNA was achieved by melting of dsDNA molecules in solution with the integrated heater, allowing detection of DNA sequences specific to breast and colorectal cancers with a non-specific binding control. Following detection, the array surface could be renewed via high temperature (95 °C) stripping using the integrated heating element. This versatile and simple method for prototyping devices shows potential for further development of highly integrated, multi-functional bioanalytical devices. 相似文献
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Miller PR Gittard SD Edwards TL Lopez DM Xiao X Wheeler DR Monteiro-Riviere NA Brozik SM Polsky R Narayan RJ 《Biomicrofluidics》2011,5(1):13415
In this study, carbon fiber electrodes were incorporated within a hollow microneedle array, which was fabricated using a digital micromirror device-based stereolithography instrument. Cell proliferation on the acrylate-based polymer used in microneedle fabrication was examined with human dermal fibroblasts and neonatal human epidermal keratinocytes. Studies involving full-thickness cadaveric porcine skin and trypan blue dye demonstrated that the hollow microneedles remained intact after puncturing the outermost layer of cadaveric porcine skin. The carbon fibers underwent chemical modification in order to enable detection of hydrogen peroxide and ascorbic acid; electrochemical measurements were demonstrated using integrated electrode-hollow microneedle devices. 相似文献
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Nicole Megan Edwards 《Early education and development》2014,25(8):1219-1247
Research Findings: There is growing acknowledgment of the need for parenting interventions to address early-onset behavior and emotional concerns. Favorable child outcomes have been linked to parents’ responsiveness and positive expressiveness. Given the theoretical and empirical link between perceptions and actual behavior, Head Start mothers (n = 114) participated in an investigation to examine factors that may be associated with level of self-reported maternal expressiveness. Participants completed the Self-Expressiveness in the Family Questionnaire (Halberstadt, Cassidy, Stifter, Parke, & Fox, 1995). Analysis suggested that self-reported high negative expressiveness was linked with raising a preschooler with perceived internalizing and externalizing behaviors, high parenting stress, and obtaining a post–high school degree. A perceived view of being low in positive expressiveness, in contrast, correlated with different variables, including raising a preschooler with a diagnosed delay, not having any child receive specialized services, raising only 1 child, dropping out of high school, and not receiving behavior advice from Head Start staff. Practice or Policy: Implications for examining self-reported low positive and high negative expressiveness as separate constructs with possibly different intervention pathways are discussed. 相似文献
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Lana Edwards Santoro Michael D. Coyne Deborah C. Simmons 《Learning disabilities research & practice》2006,21(2):122-133
Abstract In this article, we describe the development and evaluation of a beginning spelling intervention for young children at risk of reading disability. We first summarize the literature that supports beginning spelling as an ideal method for strategically integrating the beginning reading big ideas of phonemic awareness and alphabetic understanding. We then summarize the literature on effective instructional principles for students at risk of reading disability. Next, we describe how instructional design was applied to the development of an intervention for young children at risk of reading disability, then summarize the findings of an experimental study supporting the effectiveness of this intervention. Finally, we provide selected examples from the spelling intervention to illustrate the findings' translation into instructional practice. 相似文献
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Ken Edwards 《Higher Education in Europe》2006,31(3):289-293
This paper describes briefly the academic tenure and its legislative terms in the UK, over the last twenty years. The author refers to the current wider situation/trends in the international higher education arena of two categories of academic staff – “established” staff (on permanent contract) and fixed‐term staff – and to how their levels of protection can have an impact on the overall health of the universities and their academic freedom. 相似文献
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