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排序方式: 共有534条查询结果,搜索用时 46 毫秒
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Stefanie Demming Gena Peterat Andreu Llobera Hannah Schmolke Alexander Bruns Michael Kohlstedt Ala‘aldeen Al-Halhouli Claus-Peter Klages Rainer Krull Stephanus Büttgenbach 《Biomicrofluidics》2012,6(3)
This paper presents a vertically positioned microfluidic system made of poly(dimethylsiloxane) (PDMS) and glass, which can be applied as a microbubble column (μBC) for biotechnological screening in suspension. In this μBC, microbubbles are produced in a cultivation chamber through an integrated nozzle structure. Thus, homogeneous suspension of biomass is achieved in the cultivation chamber without requiring additional mixing elements. Moreover, blockage due to produced carbon dioxide by the microorganisms—a problem predominant in common, horizontally positioned microbioreactors (MBRs)—is avoided, as the gas bubbles are released by buoyancy at the upper part of the microsystem. The patterned PDMS layer is based on an optimized two-lithographic process. Since the naturally hydrophobic PDMS causes problems for the sufficient production of microbubbles, a method based on polyelectrolyte multilayers is applied in order to allow continuous hydrophilization of the already bonded PDMS-glass-system. The μBC comprises various microelements, including stabilization of temperature, control of continuous bubble formation, and two optical configurations for measurement of optical density with two different sensitivities. In addition, the simple and robust application and handling of the μBC is achieved via a custom-made modular plug-in adapter. To validate the scalability from laboratory scale to microscale, and thus to demonstrate the successful application of the μBC as a screening instrument, a batch cultivation of Saccharomyces cerevisiae is performed in the μBC and compared to shake flask cultivation. Monitoring of the biomass growth in the μBC with the integrated online analytics resulted in a specific growth rate of 0.32 h−1, which is almost identical to the one achieved in the shake flask cultivation (0.31 h−1). Therefore, the validity of the μBC as an alternative screening tool compared to other conventional laboratory scale systems in bioprocess development is proven. In addition, vertically positioned microbioreactors show high potential in comparison to conventional screening tools, since they allow for high density of integrated online analytics and therefore minimize time and cost for screening and guarantee improved control and analysis of cultivation parameters. 相似文献
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Metacognition and Self-Regulated Learning Constructs 总被引:1,自引:0,他引:1
Rayne A. Sperling Bruce C. Howard Richard Staley Nelson DuBois 《Educational Research and Evaluation》2013,19(2):117-139
Demographic variables, findings from the Metacognitive Awareness Inventory (Schraw & Dennison, 1994), the Learning Strategies Survey (Kardash & Amlund, 1991), and the Motivated Strategies for Learning Questionnaire (Pintrich, Smith, Garcia, & McKeachie, 1991), as well as accuracy ratings of test performance, were examined in 2 studies. Findings indicated convergence of self-report measures of metacognition, significant correlations between metacognition and academic monitoring, negative correlations between self-reported metacognition and accuracy ratings, and positive correlations between metacognition and strategy use and metacognition and motivation. Limitations of the studies and implications for theory development and future research are discussed. 相似文献
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Matthew S. Nelson Alese Wooditch Shaun L. Gabbidon 《Journal of Criminal Justice Education》2013,24(1):16-33
There is a need to explore trends in data use by researchers in criminology to assess the limitations of the knowledge base in the discipline. The current study explores the use of data published in three top criminology journals over a 10-year period to review: (1) data types (primary, secondary, or both), (2) the age of the data, and (3) whether scholars using data at least a decade old mention it as a limitation. The study found that the time dimension of data varied by publication source. A heavy reliance on secondary data was observed across all journals. Studies using only secondary data tended to have older data on average when compared to other data types. A majority of articles using data at least a decade old did not mention it is a shortcoming. The paper concludes by discussing advantages and disadvantages of relying on secondary data within the discipline of criminology. 相似文献
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Tammy J. Rhine Dawna M. Milligan Lynne R. Nelson 《Community College Journal of Research & Practice》2013,37(6):443-453
Community college students transferring to a 4-year college or university face a variety of challenges. Social and academic issues can pose potential hurdles to graduating with a bachelor's degree for these students. Community colleges and 4-year institutions must work closely together to create more efficient and effective partnerships for students transitioning through the higher education system. This article makes suggestions for community college staff and faculty members to help transfer students successfully transition into university life and complete a bachelor's degree. 相似文献
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Nelson A. Reynoso 《Community College Journal of Research & Practice》2013,37(4-6):391-434
This study examined how Dominican English-language learners at Bronx Community College achieved academic resiliency despite facing personal, academic, and environmental adversities. Using individual interviews, data were analyzed from the perception of students. A resilient-based model emerged that sheds light on the personal barriers experienced by Dominican English-language learners in an urban community college and the facilitating factors which fostered educational resiliency. The author found seven resiliency factors that contributed to the academic achievement of Dominican English-language learners: (a) faculty support, (b) tutoring support, (c) counseling support, (d) peer support, (e) family support, (f) self-motivation, and (g) bi-cultural identity development. 相似文献