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51.
Cryopreservation of human red blood cells (RBCs) in the presence of 40% glycerol allows a shelf-life of 10 years, as opposed to only 6 weeks for refrigerated RBCs. Nonetheless, cryopreserved blood is rarely used in clinical therapy, in part because of the requirement for a time-consuming (∼1 h) post-thaw wash process to remove glycerol before the product can be used for transfusion. The current deglycerolization process involves a series of saline washes in an automated centrifuge, which gradually removes glycerol from the cells in order to prevent osmotic damage. We recently demonstrated that glycerol can be extracted in as little as 3 min without excessive osmotic damage if the composition of the extracellular solution is precisely controlled. Here, we explore the potential for carrying out rapid glycerol extraction using a membrane-based microfluidic device, with the ultimate goal of enabling inline washing of cryopreserved blood. To assist in experimental design and device optimization, we developed a mass transfer model that allows prediction of glycerol removal, as well as the resulting cell volume changes. Experimental measurements of solution composition and hemolysis at the device outlet are in reasonable agreement with model predictions, and our results demonstrate that it is possible to reduce the glycerol concentration by more than 50% in a single device without excessive hemolysis. Based on these promising results, we present a design for a multistage process that is predicted to safely remove glycerol from cryopreserved blood in less than 3 min. 相似文献
52.
Chris Higgins 《Educational theory》2003,53(2):131-154
53.
Sheila Watt Elizabeth Horn & Cassie Higgins 《British Journal of Special Education》1997,24(3):115-123
This article examines the efficacy of policy and practice, within one Scottish region, of education about sexuality in secondary schools. The primary focus is on teaching sexuality to pupils with recorded learning difficulties, with a particular emphasis on the concepts of inclusion, normalisation and integration. The authors are Sheila Watt, Lecturer in the School of Education, University of Dundee; Elizabeth Horn, a teacher of pupils with special educational needs; and Cassie Higgins, a research assistant also based in the School of Education at the University of Dundee. 相似文献
54.
Collette Higgins 《English in Education》2002,36(1):25-37
This article is the result of working with several schools concerned with improving achievement in boys' writing. It begins by describing a range of effective teaching and learning strategies, observed in my role as a Literacy Consultant, which had a positive influence on writers especially boys. The article goes on to explore how a small group of boys, described by their teacher as ‘reluctant writers’, were encouraged by the use of film text to make the most of their preferences for action‐driven narratives to improve story writing. It suggests that ‘slowing down’ this action in the author's eye gives pupils, and boys in particular, an opportunity to consider detail to enhance composition in the same way that a film director uses camera angles to capture the viewer's attention. 相似文献
55.
The changing focus of public policy. 总被引:1,自引:0,他引:1
56.
Leslie C. Higgins 《Educational technology research and development : ETR & D》1979,27(4):265-280
It is important that children learn to draw inferences from pictures. But can that ability be taught? This study sought to develop the picture-interpretation strategies of fourth-grade children by providing training in the use of such verbal guidelines as “Help yourself to all the given information.” The program apparently helped the children produce more inferences, but did not enhance their skills in evaluating inferences at least not over the 16 30–40 minute sessions devoted to the training. 相似文献
57.
Stainton C Murphy B Higgins PG Neff JA Nyberg K Ritchie JA 《The Journal of perinatal education》1999,8(3):21-29
Health care reform has moved postbirth care into the community without assessment of how this may change parents' needs. Mother-father pairs (n = 117) in six sites in four countries were interviewed at 5 to 10 days postbirth and again between 6 and 8 weeks postbirth to determine their needs. Content analysis revealed both parents in all sites had a strong focus on self-needs throughout the postbirth period. Postpartum programs oriented solely to family-centered care may not meet the specific needs of contemporary parents. 相似文献
58.
59.
Sharon Shrock Norman Higgins 《Educational technology research and development : ETR & D》1990,38(3):77-80
The authors of this commentary take exception to several points made in an article that appeared in the most recent issue of this journal (ETR&D, Vol. 38, No. 2). Following this explanation of their concerns is a reply by the authors of the original article. 相似文献
60.
The authors introduce an innovative technique that can be included in marriage and family counselor education curricula. Feature films are discussed as an educational technique for the instruction and development of perceptual, conceptual, and executive counseling skills. They also outline the advantages for using film in counselor education, present various strategies for using them in the classroom, and offer examples of how they have integrated movies into introductory marriage and family courses. 相似文献