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101.
A menaquinone-7 (MK-7) high-producing strain needs to be isolated to increase MK-7 production, in order to meet a requirement of MK-7 given the low MK-7 content in food products. This article focuses on developing MK-7 high-producing strains via screening and mutagenesis by an atmospheric and room temperature plasma (ARTP) mutation breeding system. We isolated an MK-7-producing strain Y-2 and identified it as Bacillus amyloliquefaciens, which produced (7.1±0.5) mg/L of MK-7 with maize meal hydrolysate as carbon source. Then, an MK-7 highproducing strain B. amyloliquefaciens H.β.D.R.-5 with resistance to 1-hydroxy-2-naphthoic acid, β-2-thienylalanine, and diphenylamine was obtained from the mutation of the strain Y-2 using an ARTP mutation breeding system. Using strain H.β.D.R.-5, efficient production of MK-7 was achieved ((30.2±2.7) mg/L). In addition, the effects of nitrogen sources, prenyl alcohols, and MgSO4 on MK-7 production were investigated, suggesting that soymeal extract combined with yeast extract, isopentenol, and MgSO4 was beneficial. Under the optimized condition, the MK-7 production and biomass-specific yield reached (61.3±5.2) mg/L and 2.59 mg/L per OD600 unit respectively in a 7-L fermenter. These results demonstrated that strain H.β.D.R.-5 has the capacity to produce MK-7 from maize meal hydrolysate, which could reduce the substrate cost. 相似文献
102.
互联网对高校思想政治教育的影响 总被引:2,自引:0,他引:2
互联网的出现和发展,既给高校思想政治教育带来了机遇,也形成了严峻的挑战.本文对机遇和挑战进行具体分析,认为应该扬长避短,主动出击,采取相关对策,切实解决问题. 相似文献
103.
To assess the influence of cyclosporin A (CsA) and tacrolimus (FK506) on mycophenolic acid (MPA) and correlation analysis
of the pharmacokinetic parameters and patient characteristics, clinical outcome in Chinese kidney transplant recipients, the
pharmacokinetics of 1000 mg mycophenolate mofetil (MMF) twice daily was measured by high-performance liquid chromatography
(HPLC). PKS (Pharmaceutical Kinctics Software) 1.0.2 software package was used for the calculation of pharmacokinetic parameters.
The meanC
max,t
max, and AUC(0–12) were (21.88±10.52) μg/ml, (1.20±0.95) h, and (52.546±13.215) μg·h/ml, respectively. The level of AUC(0–12) in the FK506 group was significantly higher than that in the CsA group. MPA appeared not to be affected by renal function.
MPA AUC(0–12) showed statistically significant difference according to the patient's gender.
Project (No. 20040462) supported by the Foundation of Education Bureau of Zhejiang Province, China 相似文献
104.
The deviation of flow characteristics from the predictions of the conventional theory for microtubes was attributed to the change of fluid viscosity resulted from the interactions between the molecules on solid wall and in fluid. The degree of this departure is dependent on the microtubes materials. A concept of equivalent thickness with which conventional theory can be used to predict the flow in microtubes without modifying the fluid viscosity was put forward. The values of equivalent thickness for fused silica and stainless steel materials were determined as 1.8 μm and 1.5 μm, respectively, by repeated numerical simulation. 相似文献
105.
舒建中 《西南师范大学学报(人文社会科学版)》2006,32(1):159-163
作为对捷克斯洛伐克“二月事件”的反应,美国在关贸总协定机制下的对捷政策随即发生了根本性转变,其战略意图就是孤立和排斥捷克斯洛伐克,其主要目标是寻求中止对捷克斯洛伐克的关贸总协定义务。凭借美国在关贸总协定机制中占据主导地位的影响力并经过积极的外交努力,美国的战略意图和政策目标基本实现。因此,1948—1951年问美国在关贸总协定机制下的对捷政策再次表明,美国在一定意义上已经将1947年关贸总协定机制作为推行“冷战”战略和遏制政策的有力工具。 相似文献
106.
申屠青松 《南京师范大学文学院学报》2007,(4):75-79
明代宋诗选本现可考者约有十五家。它们虽是对时人过度轻视宋诗的反驳,但也没有超越崇唐抑宋的时代风气,在选诗标准上多持以唐存宋的态度。明代宋诗选本保留了不少珍贵的宋诗文献,成为清代宋诗选本的文献来源之一,对清代宋诗复兴起过重要作用,但作为文献又有诸多缺陷,给清代选本带来不好的影响。 相似文献
107.
Shan Li Yun-chao Zheng Hai-rui Cui Hao-wei Fu Qing-yao Shu Jian-zhong Huang 《Journal of Zhejiang University. Science. B》2016,17(12):905-915
Mutation breeding is based on the induction of genetic variations; hence knowledge of the frequency and type of induced mutations is of paramount importance for the design and implementation of a mutation breeding program. Although γ ray irradiation has been widely used since the 1960s in the breeding of about 200 economically important plant species, molecular elucidation of its genetic effects has so far been achieved largely by analysis of target genes or genomic regions. In the present study, the whole genomes of six γ-irradiated M2 rice plants were sequenced; a total of 144–188 million high-quality (Q>20) reads were generated for each M2 plant, resulting in genome coverage of >45 times for each plant. Single base substitution (SBS) and short insertion/deletion (Indel) mutations were detected at the average frequency of 7.5×10?6–9.8×10?6 in the six M2 rice plants (SBS being about 4 times more frequent than Indels). Structural and copy number variations, though less frequent than SBS and Indel, were also identified and validated. The mutations were scattered in all genomic regions across 12 rice chromosomes without apparent hotspots. The present study is the first genome-wide single-nucleotide resolution study on the feature and frequency of γ irradiation-induced mutations in a seed propagated crop; the findings are of practical importance for mutation breeding of rice and other crop species. 相似文献
108.
依据人力资源管理专业培养应用型管理人才目标的要求,提出在进行绩效管理理论教学的同时,加大实践教学环节,优化现有教学模式,提高学生绩效管理的操作能力。 相似文献
109.
钧釉最好的传承方式是创新,但钧釉的创新有三个前提:必须以铜、铁为主要着色剂;必须是高温还原烧成;必须是自然窑变。新钧釉的特点是:釉中除加有铁、铜等着色剂外,还加有其他的着色元素;窑变色彩、纹理、斑点、意境等变化更加丰富;适当地突出了缺陷美;具有时代气息和现代艺术美感。钧釉创新的方法,一是调整烧成方法,二是不断调整釉的配方。只要不断地试验、摸索、研究、总结,钧釉品种定能不断推陈出新。钧瓷的窑变永无止境,钧釉的创新亦永无止境。 相似文献
110.
提出了潜在狄利赫雷分布模型与自然语言信息处理相关技术相结合的一种挖掘Twitter中中文微博的热点主题词组的方法.选取了20923条中文Tweets作为样本,获取了相关热点的主题词组,与预期的效果基本吻合,表明该模型具有较好的热点识别效果和对主题进行描述的能力. 相似文献